LOCUS       pCMV-3UTR        4363 bp DNA     circular SYN 27-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-3UTR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Trial User hesko
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2023 from SnapGene Viewer 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..574
                     /label=P CMV
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        66..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     597..622
                     /label=NheI-pHuF
     RBS             603..611
                     /label=Kozak
                     /note="Kozak"
     sig_peptide     612..671
                     /label=Signal peptide IGKV3 family
                     /label=Signal peptide
                     /note="/dnas_title=Signal peptide
                     /vntifkey=4"
     misc_feature    672..688
                     /label=MCS
     3'UTR           689..892
                     /label=EF1alpha 3'UTR
                     /note="EF1alpha 3'UTR"
     primer_bind     complement(871..898)
                     /label=EF1UTR-PvuIR
     polyA_signal    1157..1278
                     /label=SV40 polyA
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1285..1740)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1767..1869
                     /gene="bla"
                     /label=P bla
     promoter        1873..2230
                     /label=P SV40
                     /note="SV40 enhancer and early promoter"
     CDS             2265..3059
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3060..3506
                     /label=HSV TK polyA
     misc_feature    3644..4311
                     /label=pUC origin
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgccgccac catggaagcc ccagcgcagc ttctcttcct cctgctactc tggctccctg
      661 ataccaccgg tactagtgaa ttctcgagaa tattatccct aatacgtgcc accccactct
      721 taatcagtgg tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag
      781 tagtaaaaga ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat
      841 gttttgtgga ccactttggt tttctttttt gcgtgtggca gttttaagtt atcgatcgcc
      901 cttcccaaca gttgcgcagc ctgaatggcg aatggagatc caatttttaa gtgtataatg
      961 tgttaaacta ctgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt
     1021 caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga
     1081 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1141 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1261 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     1321 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     1381 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     1441 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     1501 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     1561 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     1621 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     1681 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     1741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1861 aaaggaagaa tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     1921 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1981 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2041 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2101 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2161 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2221 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     2281 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     2341 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     2401 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     2461 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     2521 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     2581 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     2641 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     2701 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagaacat caggggctcg
     2761 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     2821 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     2881 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     2941 gtgatattgc tgaagaactt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     3001 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     3061 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     3121 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     3181 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     3241 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     3301 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     3361 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     3421 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     3481 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     3541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3781 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4261 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4321 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcc
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