LOCUS       AAV-P3-evoCjCas9-ABE8e_gRNA        7898 bp ds-DNA     circular     18-MAY-2023
DEFINITION  .
ACCESSION   urn.local...4z-g9pa7ei
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/lukas/Downloads
            Filename :: aav-cterm714-spcas-syn-pa (1).gb
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /standard_name="AAV2 ITR"
     regulatory      131..448
                     /note="Geneious type: promoter"
                     /standard_name="P3"
     intron          451..583
                     /note="Promega's chimeric intron"
                     /standard_name="chI"
     CDS             594..650
                     /standard_name="BPNLS"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
     CDS             651..1148
                     /standard_name="TadAmax10 (ABE8)"
     misc_feature    1149..1244
                     /note="Geneious type: Misc"
                     /standard_name="3xGGS-XTEN-3xGGS"
                     /note="Geneious type: CDS"
     CDS             1245..4193
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="evoCjCas9(D8A)"
     CDS             4194..4259
                     /standard_name="NLS"
     regulatory      4270..4493
                     /note="Geneious type: terminator"
                     /note="Sequence used for polyadenlyation and termination
                     of transcripts"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bGH PA"
     misc_feature    complement(4507..4586)
                     /standard_name="optimized CjCas scaffold"
     misc_feature    complement(4587..4709)
                     /standard_name="SPACER"
     regulatory      complement(4713..4958)
                     /note="Geneious type: promoter"
                     /standard_name="U6 promoter"
     repeat_region   4959..5088
                     /standard_name="AAV2 ITR"
     rep_origin      5265..5720
     regulatory      5746..5850
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
     CDS             5851..6711
                     /standard_name="AmpR"
     rep_origin      6882..7470
     protein_bind    7758..7779
                     /standard_name="CAP binding site"
     regulatory      7794..7824
                     /note="Geneious type: promoter"
                     /standard_name="lac promoter"
     protein_bind    7832..7848
                     /standard_name="lac operator"
ORIGIN      
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct accggcgcgc cgggggaggc tgctggtgaa tattaaccaa ggtcacccca
      181 gttatcggag gagcaaacag gggctaagtc cacacgcgtg gtaccgtctg tctgcacatt
      241 tcgtagagcg agtgttccga tactctaatc tccctaggca aggttcatat ttgtgtaggt
      301 tacttattct ccttttgttg actaagtcaa taatcagaat cagcaggttt ggagtcagct
      361 tggcagggat cagcagcctg ggttggaagg agggggtata aaagcccctt caccaggaga
      421 agccgtcaca cagatccaca agctcctggg gtaagtatca aggttacaag acaggtttaa
      481 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      541 cctattggtc ttactgacat ccactttgcc tttctctcca cagggccgcc accatgaaac
      601 ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc tctgaggtgg
      661 agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg gcacgggatg
      721 agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct
      781 ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg gccctgagac
      841 agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac gtgacattcg
      901 agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc gtggtgtttg
      961 gcgtgaggaa ctcaaaaaga ggcgccgcag gctccctgat gaacgtgctg aactaccccg
     1021 gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt gccgccctgc
     1081 tgtgcgattt ctatcggatg cctagacagg tgttcaatgc tcagaagaag gcccagagct
     1141 ccatcaactc cggaggatct agcggaggct cctctggctc tgagacacct ggcacaagcg
     1201 agagcgcaac acctgaaagc agcgggggca gcagcggggg gtcagcccgc atcctggcct
     1261 ttgccatcgg catcagcagc atcggatggg ccttcagcga gaatgacgag ctgaaggact
     1321 gcggtgtgag aatctttaca aaggtcgaga accccaagac cggcgaaagc ctggcgctgc
     1381 caagacggct ggcccggtct gctcggaaga gatacgcccg gagaaaagcc agactgaatc
     1441 acctgaaaca cctgatcgcc aacgagttca aactgaacta cgaggactac cagagcttcg
     1501 atgagagctt ggctaaggcc tacaaaggca gcctgatcag cccctacgag ctgagattca
     1561 gagctctgaa tgagctcctg agcaagcagg acttcgctag agtgatcctg cacatcgcca
     1621 agcgaagagg ctacgacgac atcaagaact ctgatgacaa ggagaagggc gccattctga
     1681 aagccatcaa gcagaacgaa gagaaactgg ccaattacca gagcgtgggc gagtacctgt
     1741 acaaggagta cttccagaag ttcaaagaaa atagcaaaga gttcaccaac gtgcggaaca
     1801 agaaggagtc ttatgaaaga tgtatcgccc agagcttcct gaaagacgag ctgaaactga
     1861 tcttcaagaa gcaaagagag tttggcttca gcttcagcaa aaaatttgaa gaagaggtgc
     1921 tgtctgtcgc cttctacaag agggctctga aggacttcag ccacctggtg ggcaattgca
     1981 gctttttcac agacgaaaag cgggccccta agaacagccc tctggccttc atgttcgtgg
     2041 ctctgaccag aatcatcaac ctgctgaaca acctgaaaaa taccgagggc atcctctata
     2101 ccaaggacga tctgaacgcc ctgctgaatg aggtgctcaa gaatggcacc ctgacctaca
     2161 agcaaacaaa aaaactgctg ggcctgtctg acgactacga gtttaaaggc gagaagggca
     2221 cctatttcat cgaatttaag aagtacaagg aattcatcaa ggcactgggc gaacacaacc
     2281 tgtctcagga cgacctgaac gagatcgcca aggacatcac cctgatcaag gatgagatca
     2341 agctgaaaaa agctctggcc aagtacgacc tgaatcaaaa ccagatcgac agcttaagca
     2401 agctggaatt taaggatcac ctgaacatct cctttaaggc cctgaagctg gtgaccccac
     2461 tgatgctgga aggaaagaag tacgacgaag catgcaacga gcttaacctg aaggttgcta
     2521 tcaacgagga taagaaggat ttcctgcctg cctttaacga gacatactac aaggatgagg
     2581 tgaccaaccc cgtggtgctg agagctatca aagagtacag aaaggtgctg aacgccctgc
     2641 tgaagaagta cggcaaggtc cacaagatca atattgagct ggcccgggag gttggaaaga
     2701 accactctca gagagcaaag atcgagaagg agcaaaacga gaactacaaa gcgaagaagg
     2761 acgccgaact ggagtgcgag aagcttggcc tgaagatcaa ctctaagaat atcctgaaac
     2821 tcagactttt caaagaacag aaggaattct gtgcctacag cggcgagaag atcaaaattt
     2881 ctgacctgca ggatgaaaag atgctggaga tcgaccacat ctacccttac tccagaagct
     2941 tcgacgacag ctatatgaac aaagtgctgg tgttcacaaa gcagaaccag gagaagctga
     3001 atcagacccc tttcgaggcc ttcggcaatg actccgccaa gtggcagaaa atcgaggtgc
     3061 tggccaaaaa cctgccaacc aagaaacaga agagaatcct cgacaagaac tacaaggaca
     3121 aggaacagaa gaacttcaag gatcggaacc tgaacgacac ccggtacatc gccaggctgg
     3181 tgttaaatta caccaaggac tacctggatt tcctgcccct gagcgacgac gagaacacca
     3241 agctgaacga cacacagaag ggcagcaagg tgcacgtgga agccaagagc ggcatgctga
     3301 ccagcgcact gcgccacact tggggcttca gcgctaagga ccggaacaac cacctgcatc
     3361 acgccatcga tgccgtgatc atagcctacg ccaacaactc aatcgtgaaa gctttcagtg
     3421 actttaagaa agaacaggag agcaactctg ccgaactgta cgccaagaaa attagcgagc
     3481 tggactacaa gaacaaacgg aagttcttcg aacctttctc aggatttaga cagaaggtgc
     3541 tggataagat cgatgaaatc ttcgtgtcca agcccgagag aaagaagcct agcggagccc
     3601 tgcacaagga aaccttcaga aaggaagaag agttctacca gtcttatggg ggcaaagaag
     3661 gcgtgctgaa ggccctggaa ctcggcaaga tcagaaaggt gaagggaaag attgtgaaga
     3721 acggcgacat gttcagagtg gacatcttca agcacaagaa gaccaacaag ttctatgccg
     3781 tgcctatcta cacaatggat ttcgccctga aagtgctgcc taacaaggct gtcgccagat
     3841 ctaagaaggg cgagatcaag gactggatcc tgatggacga aaactacgag ttctgcttca
     3901 gcctgtacaa ggacagcctg atcctgatcc aaacaaagaa gatgcaggag cctgaattcg
     3961 tgtactacaa cgccttcaca agcagcaccg tgagcctgat cgtgtctaaa catgataaca
     4021 agttcgaaac cctgtccaag aaccaaaaga tcctgttcaa gaacgccaac gagaaggaag
     4081 tgatcgccaa gggcatcggc attcagaatc tgaaggtgtt cgagaaatat atcgtgtccg
     4141 ctctgggaga ggttacaaag gccgagtttc ggcagagaga agattttaag aagtctggcg
     4201 gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg aaagtctaat
     4261 agatctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4321 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4381 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4441 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggctcgag
     4501 cggcccaaaa aaagcggttt taggggattg taaccccgca gagtcccgca aactctttat
     4561 tctagtccct tttcagggac tagaacagag acgtacaaaa aagagcaaga agctaaaaaa
     4621 gatttaaaaa ttatttttag cgcagttaat ggaacaggaa ctaaatttac cccaaaatgt
     4681 attttgtgtg ctcatttaca accgtctccg gtgtttcgtc ctttccacaa gatatataaa
     4741 gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat
     4801 aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact
     4861 aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt
     4921 atatgcaaat atgaaggaat catgggaaat aggccctcag gaacccctag tgatggagtt
     4981 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     5041 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc
     5101 taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5161 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5221 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5281 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5341 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5401 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5461 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5521 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5581 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5641 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5701 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5821 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5881 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5941 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6001 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6061 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6121 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6181 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6241 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6301 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6361 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6421 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6481 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6541 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6601 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6661 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6721 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6781 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6841 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6901 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6961 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7021 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7081 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7141 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7201 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7261 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7321 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7381 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7441 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7501 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7561 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7621 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7681 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7741 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7801 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7861 aacagctatg accatgatta cgccagattt aattaagg
//
