LOCUS       AAV-hSyn-evoCjCas9-ABE8e_gRNA        7915 bp ds-DNA     circular     18-MAY-2023
DEFINITION  .
ACCESSION   urn.local...ab-g9pdgkc
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/lukas/Downloads
            Filename :: aav-cterm714-spcas-syn-pa (1).gb
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /standard_name="AAV2 ITR"
     misc_feature    130
                     /standard_name="Xbal"
     regulatory      131..600
                     /note="Geneious type: promoter"
                     /standard_name="hSyn1 promoter-470"
     CDS             611..667
                     /standard_name="BPNLS"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
     CDS             668..1165
                     /standard_name="TadAmax10 (ABE8)"
     misc_feature    1166..1261
                     /note="Geneious type: Misc"
                     /standard_name="3xGGS-XTEN-3xGGS"
                     /note="Geneious type: CDS"
     CDS             1262..4210
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="evoCjCas9(D8A)"
     CDS             4211..4276
                     /standard_name="NLS"
     regulatory      4287..4510
                     /note="Geneious type: terminator"
                     /note="Sequence used for polyadenlyation and termination
                     of transcripts"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bGH PA"
     misc_feature    complement(4524..4603)
                     /standard_name="optimized CjCas scaffold"
     misc_feature    complement(4604..4726)
                     /standard_name="SPACER"
     regulatory      complement(4730..4975)
                     /note="Geneious type: promoter"
                     /standard_name="U6 promoter"
     repeat_region   4976..5105
                     /standard_name="AAV2 ITR"
     rep_origin      5282..5737
     regulatory      5763..5867
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
     CDS             5868..6728
                     /standard_name="AmpR"
     rep_origin      6899..7487
     protein_bind    7775..7796
                     /standard_name="CAP binding site"
     regulatory      7811..7841
                     /note="Geneious type: promoter"
                     /standard_name="lac promoter"
     protein_bind    7849..7865
                     /standard_name="lac operator"
ORIGIN      
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      181 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      241 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      301 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      361 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      421 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      481 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      541 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga
      601 ggccgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg
      661 gaaagtctct gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc
      721 caagagggca cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag
      781 agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga
      841 aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac
      901 cctgtacgtg acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat
      961 cggccgcgtg gtgtttggcg tgaggaactc aaaaagaggc gccgcaggct ccctgatgaa
     1021 cgtgctgaac taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga
     1081 tgaatgtgcc gccctgctgt gcgatttcta tcggatgcct agacaggtgt tcaatgctca
     1141 gaagaaggcc cagagctcca tcaactccgg aggatctagc ggaggctcct ctggctctga
     1201 gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc
     1261 agcccgcatc ctggcctttg ccatcggcat cagcagcatc ggatgggcct tcagcgagaa
     1321 tgacgagctg aaggactgcg gtgtgagaat ctttacaaag gtcgagaacc ccaagaccgg
     1381 cgaaagcctg gcgctgccaa gacggctggc ccggtctgct cggaagagat acgcccggag
     1441 aaaagccaga ctgaatcacc tgaaacacct gatcgccaac gagttcaaac tgaactacga
     1501 ggactaccag agcttcgatg agagcttggc taaggcctac aaaggcagcc tgatcagccc
     1561 ctacgagctg agattcagag ctctgaatga gctcctgagc aagcaggact tcgctagagt
     1621 gatcctgcac atcgccaagc gaagaggcta cgacgacatc aagaactctg atgacaagga
     1681 gaagggcgcc attctgaaag ccatcaagca gaacgaagag aaactggcca attaccagag
     1741 cgtgggcgag tacctgtaca aggagtactt ccagaagttc aaagaaaata gcaaagagtt
     1801 caccaacgtg cggaacaaga aggagtctta tgaaagatgt atcgcccaga gcttcctgaa
     1861 agacgagctg aaactgatct tcaagaagca aagagagttt ggcttcagct tcagcaaaaa
     1921 atttgaagaa gaggtgctgt ctgtcgcctt ctacaagagg gctctgaagg acttcagcca
     1981 cctggtgggc aattgcagct ttttcacaga cgaaaagcgg gcccctaaga acagccctct
     2041 ggccttcatg ttcgtggctc tgaccagaat catcaacctg ctgaacaacc tgaaaaatac
     2101 cgagggcatc ctctatacca aggacgatct gaacgccctg ctgaatgagg tgctcaagaa
     2161 tggcaccctg acctacaagc aaacaaaaaa actgctgggc ctgtctgacg actacgagtt
     2221 taaaggcgag aagggcacct atttcatcga atttaagaag tacaaggaat tcatcaaggc
     2281 actgggcgaa cacaacctgt ctcaggacga cctgaacgag atcgccaagg acatcaccct
     2341 gatcaaggat gagatcaagc tgaaaaaagc tctggccaag tacgacctga atcaaaacca
     2401 gatcgacagc ttaagcaagc tggaatttaa ggatcacctg aacatctcct ttaaggccct
     2461 gaagctggtg accccactga tgctggaagg aaagaagtac gacgaagcat gcaacgagct
     2521 taacctgaag gttgctatca acgaggataa gaaggatttc ctgcctgcct ttaacgagac
     2581 atactacaag gatgaggtga ccaaccccgt ggtgctgaga gctatcaaag agtacagaaa
     2641 ggtgctgaac gccctgctga agaagtacgg caaggtccac aagatcaata ttgagctggc
     2701 ccgggaggtt ggaaagaacc actctcagag agcaaagatc gagaaggagc aaaacgagaa
     2761 ctacaaagcg aagaaggacg ccgaactgga gtgcgagaag cttggcctga agatcaactc
     2821 taagaatatc ctgaaactca gacttttcaa agaacagaag gaattctgtg cctacagcgg
     2881 cgagaagatc aaaatttctg acctgcagga tgaaaagatg ctggagatcg accacatcta
     2941 cccttactcc agaagcttcg acgacagcta tatgaacaaa gtgctggtgt tcacaaagca
     3001 gaaccaggag aagctgaatc agaccccttt cgaggccttc ggcaatgact ccgccaagtg
     3061 gcagaaaatc gaggtgctgg ccaaaaacct gccaaccaag aaacagaaga gaatcctcga
     3121 caagaactac aaggacaagg aacagaagaa cttcaaggat cggaacctga acgacacccg
     3181 gtacatcgcc aggctggtgt taaattacac caaggactac ctggatttcc tgcccctgag
     3241 cgacgacgag aacaccaagc tgaacgacac acagaagggc agcaaggtgc acgtggaagc
     3301 caagagcggc atgctgacca gcgcactgcg ccacacttgg ggcttcagcg ctaaggaccg
     3361 gaacaaccac ctgcatcacg ccatcgatgc cgtgatcata gcctacgcca acaactcaat
     3421 cgtgaaagct ttcagtgact ttaagaaaga acaggagagc aactctgccg aactgtacgc
     3481 caagaaaatt agcgagctgg actacaagaa caaacggaag ttcttcgaac ctttctcagg
     3541 atttagacag aaggtgctgg ataagatcga tgaaatcttc gtgtccaagc ccgagagaaa
     3601 gaagcctagc ggagccctgc acaaggaaac cttcagaaag gaagaagagt tctaccagtc
     3661 ttatgggggc aaagaaggcg tgctgaaggc cctggaactc ggcaagatca gaaaggtgaa
     3721 gggaaagatt gtgaagaacg gcgacatgtt cagagtggac atcttcaagc acaagaagac
     3781 caacaagttc tatgccgtgc ctatctacac aatggatttc gccctgaaag tgctgcctaa
     3841 caaggctgtc gccagatcta agaagggcga gatcaaggac tggatcctga tggacgaaaa
     3901 ctacgagttc tgcttcagcc tgtacaagga cagcctgatc ctgatccaaa caaagaagat
     3961 gcaggagcct gaattcgtgt actacaacgc cttcacaagc agcaccgtga gcctgatcgt
     4021 gtctaaacat gataacaagt tcgaaaccct gtccaagaac caaaagatcc tgttcaagaa
     4081 cgccaacgag aaggaagtga tcgccaaggg catcggcatt cagaatctga aggtgttcga
     4141 gaaatatatc gtgtccgctc tgggagaggt tacaaaggcc gagtttcggc agagagaaga
     4201 ttttaagaag tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa
     4261 gaagaggaaa gtctaataga tctcgactgt gccttctagt tgccagccat ctgttgtttg
     4321 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4381 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4441 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4501 gggctctatg gctcgagcgg cccaaaaaaa gcggttttag gggattgtaa ccccgcagag
     4561 tcccgcaaac tctttattct agtccctttt cagggactag aacagagacg tacaaaaaag
     4621 agcaagaagc taaaaaagat ttaaaaatta tttttagcgc agttaatgga acaggaacta
     4681 aatttacccc aaaatgtatt ttgtgtgctc atttacaacc gtctccggtg tttcgtcctt
     4741 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
     4801 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
     4861 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
     4921 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcaggaa
     4981 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     5041 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     5101 cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5161 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5221 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5281 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5341 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5401 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5461 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5521 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5581 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5641 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5701 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5821 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7501 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7561 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7621 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7681 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7741 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7801 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7861 aacaatttca cacaggaaac agctatgacc atgattacgc cagatttaat taagg
//
