LOCUS       Exported               13439 bp ds-DNA     circular SYN 15-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pZG61
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   nkxugy07
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Donor vector: pDonr207(gen, 10mg/l)
            C i  (attB2) CG GAC CAC TTT GTA CAA GAA AGC TGG GTN - reverse 
            template-specific primer
            ORIGDB|GenBank
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..854
                     /label=35S
                     /note="/vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             864..2072
                     /codon_start=1
                     /label=dADARcd
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MYSPMVVPQKNVPLPIDDKSSSMELPQIHADTIGRLVLEKFMEVI
                     KGQEAYSRRKVLAGIVMTENMNFCEAKVISVSTGTKCVSGEHMSVNGAVLNDSHAEIVS
                     RRCLLKYLYAQLDLQCNQATAYQSIFVRNTDGQYPYKLKSGVHFHLYINTAPCGDARIF
                     SPHENDTGVDKHPNRKARGQLRTKIESGQGTIPVKSSDGIQTWDGVLQGQRLLTMSCSD
                     KIARWNIVGIQGSLLSSIIEPVYLHSIVLGSLLHPEHMYRAVCGRIEKSIQGLPPPYHL
                     NKPRLALVTSAEPRNQAKAPNFGINWTIGDTELEVVNSLTGRTIGGQVSRITKQAFFVK
                     YGFLMANLPGILVRKVTTDYGQTKANVKDYQIAKLELFSAFKREDLGSWLKKPIEQDEF
                     GLAE"
     misc_feature    2079..2126
                     /label=Linker
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    2135..2148
                     /label=LIC1
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     protein_bind    2154..2278
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: RIGHT"
     promoter        2303..2333
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
                     /note="This forward directional feature has 3 segments:
                        1: 2303 .. 2308 / #ffffff / -35
                        2: 2309 .. 2326 / #ffffff
                        3: 2327 .. 2333 / #ffffff / -10"
     CDS             2387..3046
                     /codon_start=1
                     /label=chl
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3388..3693
                     /codon_start=1
                     /label=ccdb
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3734..3858)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    3866..3879
                     /label=LIC2
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     terminator      3892..4534
                     /label=rbs
                     /note="/vntifkey=43"
                     /note="color: #3366cc"
     terminator      3893..4534
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
                     /note="color: #ffffff"
     primer_bind     complement(4542..4558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    4761..4785
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     misc_feature    4780..4805
                     /label=RB
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     CDS             6085..6714
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7148..8215
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8281..8475
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     misc_feature    8819..8959
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(9145..9733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9820..10614)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11039..11064
                     /label=LB
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    11039..11063
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     terminator      11131..11339
                     /label=CaMV 3'UTR (PolyA signal)
                     /label=CaMV\3'UTR\(PolyA\signal)
                     /note="/vntifkey=43"
                     /note="color: #3366cc"
     polyA_signal    11141..11315
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(11355..12380)
                     /codon_start=1
                     /label=HPT
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12416..13196)
                     /label=2*35S
                     /note="/vntifkey=30"
                     /note="color: #800000; direction: LEFT"
     promoter        complement(12448..13125)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    13316..13337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        13352..13382
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 13352 .. 13357 / #ffffff / -35
                        2: 13358 .. 13375 / #ffffff
                        3: 13376 .. 13382 / #ffffff / -10"
     protein_bind    13390..13406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     13414..13430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc accatgtact ctcctatggt cgtaccgcaa aaaaatgtgc
      901 ctctccctat agacgataaa tctagctcca tggagcttcc gcagatccac gcagacacta
      961 tcggtcgatt ggtgctggaa aagttcatgg aggttattaa aggtcaggag gcttatagcc
     1021 gtcgaaaagt tttggctgga atcgtaatga ccgaaaacat gaacttctgt gaggcaaaag
     1081 tcatttcagt gtccacgggc accaagtgtg tgtccggcga gcatatgtcc gtaaacggtg
     1141 cggtactcaa tgacagccac gccgagatag tgtcacgtcg ttgtctcctc aagtatctct
     1201 atgcccaact ggatcttcaa tgcaaccagg cgaccgctta tcagagcata tttgtgcgta
     1261 atactgacgg tcagtatccc tataaactga agtctggggt ccattttcat ctctatataa
     1321 acacggcgcc ctgcggcgat gcccgtattt tttcacctca cgagaatgac acgggagtag
     1381 ataaacatcc caaccgaaaa gcaagagggc agctccgaac caaaatcgaa tccggccagg
     1441 gcacaatccc agtgaaatct agcgacggca ttcaaacttg ggacggcgta ctacaaggcc
     1501 agcgtttgct tacgatgtcc tgctccgata aaattgctag gtggaacatc gtcggtatcc
     1561 agggctcctt gctctcttca attatagagc ccgtctatct gcattcaatt gtcttagggt
     1621 cactcttgca tcctgaacat atgtaccgag ccgtttgtgg gagaatagaa aagagtatcc
     1681 agggtctacc cccaccttac catttgaaca aacccagact ggcattggtc acatccgcag
     1741 aaccacgtaa ccaagccaag gccccgaatt ttggaataaa ctggactata ggtgacacgg
     1801 aactagaggt tgtgaactct ctcacaggca gaaccatagg agggcaagtc agccgtataa
     1861 cgaagcaggc ttttttcgta aagtatggtt tcttgatggc taatttgccc ggaatcctcg
     1921 tccgaaaggt aacgaccgat tacggtcaga caaaagcgaa tgtaaaggat taccagatcg
     1981 cgaagttgga attgttctca gccttcaaga gggaggatct tggatcatgg ctaaagaaac
     2041 caatagagca ggacgagttt ggattggctg aatctagagg aggtggctca tctggcggag
     2101 gtcagatctc gtacgcgtcc cggggcggta ccttcgacga caagaccggg cccacaagtt
     2161 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     2221 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc
     2281 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
     2341 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     2401 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     2461 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     2521 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     2581 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg
     2641 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     2701 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     2761 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     2821 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     2881 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     2941 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     3001 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg
     3061 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     3121 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     3181 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     3241 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     3301 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     3361 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     3421 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     3481 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
     3541 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     3601 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     3661 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     3721 tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     3781 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     3841 tttcttgtac aaagtggtag gggcccggct cttctcctca ctcgacctgc agagctttcg
     3901 ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca
     3961 caccagaatc ctactgagtt cgagtattat ggcattggga aacatgtttt tcttgtacca
     4021 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
     4081 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
     4141 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaaatataag agatatgcaa
     4201 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
     4261 aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt
     4321 cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca
     4381 tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt
     4441 ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt
     4501 tatgacttgc caattgattg acaacatgca tcaagcttgg cactggccgt cgttttacaa
     4561 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4621 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4681 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
     4741 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     4801 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     4861 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     4921 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     4981 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     5041 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     5101 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     5161 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     5221 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     5281 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     5341 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     5401 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     5461 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     5521 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     5581 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     5641 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     5701 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     5761 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     5821 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     5881 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     5941 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     6001 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     6061 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     6121 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     6181 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     6241 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     6301 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     6361 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     6421 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     6481 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     6541 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     6601 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     6661 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     6721 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     6781 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     6841 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     6901 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     6961 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     7021 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     7081 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     7141 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     7201 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     7261 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     7321 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     7381 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     7441 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     7501 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     7561 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     7621 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     7681 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     7741 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     7801 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     7861 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     7921 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     7981 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     8041 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     8101 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     8161 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     8221 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     8281 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     8341 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     8401 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     8461 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     8521 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     8581 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     8641 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     8701 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8761 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     8821 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     8881 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     8941 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9781 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     9841 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     9901 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     9961 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    10021 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    10081 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    10141 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    10201 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    10261 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    10321 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    10381 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    10441 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    10501 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    10561 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    10621 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
    10681 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    10741 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
    10801 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
    10861 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
    10921 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
    10981 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    11041 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    11101 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
    11161 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
    11221 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
    11281 tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc
    11341 ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
    11401 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
    11461 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
    11521 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
    11581 agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag
    11641 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
    11701 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
    11761 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
    11821 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
    11881 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
    11941 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
    12001 agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt
    12061 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc
    12121 aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
    12181 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
    12241 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
    12301 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
    12361 gtgagttcag gctttttcat atctcattgc cccccgggat ctgcgaaagc tcgagagaga
    12421 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc
    12481 ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
    12541 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    12601 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
    12661 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
    12721 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
    12781 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    12841 gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac
    12901 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    12961 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    13021 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    13081 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    13141 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
    13201 gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    13261 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    13321 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    13381 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattac
//
