LOCUS       Exported               13558 bp ds-DNA     circular SYN 15-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pZG71
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   nkxugy07
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..854
                     /label=35S
                     /note="/vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             870..2086
                     /codon_start=1
                     /label=dADARcd
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MYSPMVVPQKNVPLPIDDKSSSMELPQIHADTIGRLVLEKFMEVI
                     KGQEAYSRRKVLAGIVMTENMNFCEAKVISVSTGTKCVSGEHMSVNGAVLNDSHAEIVS
                     RRCLLKYLYAQLDLQCNQATAYQSIFVRNTDGQYPYKLKSGVHFHLYINTAPCGDARIF
                     SPHENDTGVDKHPNRKARGQLRTKIESGQGTIPVKSSDGIQTWDGVLQGQRLLTMSCSD
                     KIARWNIVGIQGSLLSSIIEPVYLHSIVLGSLLHPEHMYRAVCGRIEKSIQGLPPPYHL
                     NKPRLALVTSAEPRNQAKAPNFGINWTIGDTELEVVNSLTGRTIGGQVSRITKQAFFVK
                     YGFLMANLPGILVRKVTTDYGQTKANVKDYQIAKLELFSAFKREDLGSWLKKPIEQDEF
                     GLAEWS"
     misc_feature    2095..2108
                     /label=LIC1
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    complement(2114..2719)
                     /label=ccdB cassette
                     /label=ccdB\cassette
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: LEFT"
     CDS             complement(2137..2442)
                     /codon_start=1
                     /label=ccdB
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(2473..2575)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    2725..2738
                     /label=LIC2
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    2746..2790
                     /label=Linker
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     CDS             2797..4005
                     /codon_start=1
                     /label=dADARcd
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="YSPMVVPQKNVPLPIDDKSSSMELPQIHADTIGRLVLEKFMEVIK
                     GQEAYSRRKVLAGIVMTENMNFCEAKVISVSTGTKCVSGEHMSVNGAVLNDSHAEIVSR
                     RCLLKYLYAQLDLQCNQATAYQSIFVRNTDGQYPYKLKSGVHFHLYINTAPCGDARIFS
                     PHENDTGVDKHPNRKARGQLRTKIESGQGTIPVKSSDGIQTWDGVLQGQRLLTMSCSDK
                     IARWNIVGIQGSLLSSIIEPVYLHSIVLGSLLHPEHMYRAVCGRIEKSIQGLPPPYHLN
                     KPRLALVTSAEPRNQAKAPNFGINWTIGDTELEVVNSLTGRTIGGQVSRITKQAFFVKY
                     GFLMANLPGILVRKVTTDYGQTKANVKDYQIAKLELFSAFKREDLGSWLKKPIEQDEFG
                     LAE"
     terminator      4011..4653
                     /label=rbs
                     /note="/vntifkey=43"
                     /note="color: #3366cc"
     terminator      4012..4653
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
                     /note="color: #ffffff"
     primer_bind     complement(4661..4677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    4880..4904
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     misc_feature    4899..4924
                     /label=RB
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     CDS             6204..6833
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7267..8334
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8400..8594
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     misc_feature    8938..9078
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(9264..9852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9939..10733)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11158..11183
                     /label=LB
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    11158..11182
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     terminator      11250..11458
                     /label=CaMV 3UTR(PolyA signal)
                     /label=CaMV\3UTR(PolyA\signal)
                     /note="/vntifkey=43"
                     /note="color: #3366cc"
     polyA_signal    11260..11434
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(11474..12499)
                     /codon_start=1
                     /label=hptII
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     misc_feature    complement(12534..13314)
                     /label=2*35S
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: LEFT"
     promoter        complement(12567..13244)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    13435..13456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        13471..13501
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 13471 .. 13476 / #ffffff / -35
                        2: 13477 .. 13494 / #ffffff
                        3: 13495 .. 13501 / #ffffff / -10"
     protein_bind    13509..13525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     13533..13549
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc actagtacca tgtactctcc tatggtcgta ccgcaaaaaa
      901 atgtgcctct ccctatagac gataaatcta gctccatgga gcttccgcag atccacgcag
      961 acactatcgg tcgattggtg ctggaaaagt tcatggaggt tattaaaggt caggaggctt
     1021 atagccgtcg aaaagttttg gctggaatcg taatgaccga aaacatgaac ttctgtgagg
     1081 caaaagtcat ttcagtgtcc acgggcacca agtgtgtgtc cggcgagcat atgtccgtaa
     1141 acggtgcggt actcaatgac agccacgccg agatagtgtc acgtcgttgt ctcctcaagt
     1201 atctctatgc ccaactggat cttcaatgca accaggcgac cgcttatcag agcatatttg
     1261 tgcgtaatac tgacggtcag tatccctata aactgaagtc tggggtccat tttcatctct
     1321 atataaacac ggcgccctgc ggcgatgccc gtattttttc acctcacgag aatgacacgg
     1381 gagtagataa acatcccaac cgaaaagcaa gagggcagct ccgaaccaaa atcgaatccg
     1441 gccagggcac aatcccagtg aaatctagcg acggcattca aacttgggac ggcgtactac
     1501 aaggccagcg tttgcttacg atgtcctgct ccgataaaat tgctaggtgg aacatcgtcg
     1561 gtatccaggg ctccttgctc tcttcaatta tagagcccgt ctatctgcat tcaattgtct
     1621 tagggtcact cttgcatcct gaacatatgt accgagccgt ttgtgggaga atagaaaaga
     1681 gtatccaggg tctaccccca ccttaccatt tgaacaaacc cagactggca ttggtcacat
     1741 ccgcagaacc acgtaaccaa gccaaggccc cgaattttgg aataaactgg actataggtg
     1801 acacggaact agaggttgtg aactctctca caggcagaac cataggaggg caagtcagcc
     1861 gtataacgaa gcaggctttt ttcgtaaagt atggtttctt gatggctaat ttgcccggaa
     1921 tcctcgtccg aaaggtaacg accgattacg gtcagacaaa agcgaatgta aaggattacc
     1981 agatcgcgaa gttggaattg ttctcagcct tcaagaggga ggatcttgga tcatggctaa
     2041 agaaaccaat agagcaggac gagtttggat tggctgaatg gagctcggta ccttcgacga
     2101 caagaccggg cccctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
     2161 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     2221 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     2281 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
     2341 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     2401 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag tccctgttct
     2461 cgtcagcaaa agtttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2521 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     2581 tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac caggatttat
     2641 ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga
     2701 tgctgccaac ttagtcgagg ggcccggctc ttctcctcac tcgaccagat ctcgtacgcg
     2761 tcccggggcg gtggctcatc tggcggaggt ggatcctact ctcctatggt cgtaccgcaa
     2821 aaaaatgtgc ctctccctat agacgataaa tctagctcca tggagcttcc gcagatccac
     2881 gcagacacta tcggtcgatt ggtgctggaa aagttcatgg aggttattaa aggtcaggag
     2941 gcttatagcc gtcgaaaagt tttggctgga atcgtaatga ccgaaaacat gaacttctgt
     3001 gaggcaaaag tcatttcagt gtccacgggc accaagtgtg tgtccggcga gcatatgtcc
     3061 gtaaacggtg cggtactcaa tgacagccac gccgagatag tgtcacgtcg ttgtctcctc
     3121 aagtatctct atgcccaact ggatcttcaa tgcaaccagg cgaccgctta tcagagcata
     3181 tttgtgcgta atactgacgg tcagtatccc tataaactga agtctggggt ccattttcat
     3241 ctctatataa acacggcgcc ctgcggcgat gcccgtattt tttcacctca cgagaatgac
     3301 acgggagtag ataaacatcc caaccgaaaa gcaagagggc agctccgaac caaaatcgaa
     3361 tccggccagg gcacaatccc agtgaaatct agcgacggca ttcaaacttg ggacggcgta
     3421 ctacaaggcc agcgtttgct tacgatgtcc tgctccgata aaattgctag gtggaacatc
     3481 gtcggtatcc agggctcctt gctctcttca attatagagc ccgtctatct gcattcaatt
     3541 gtcttagggt cactcttgca tcctgaacat atgtaccgag ccgtttgtgg gagaatagaa
     3601 aagagtatcc agggtctacc cccaccttac catttgaaca aacccagact ggcattggtc
     3661 acatccgcag aaccacgtaa ccaagccaag gccccgaatt ttggaataaa ctggactata
     3721 ggtgacacgg aactagaggt tgtgaactct ctcacaggca gaaccatagg agggcaagtc
     3781 agccgtataa cgaagcaggc ttttttcgta aagtatggtt tcttgatggc taatttgccc
     3841 ggaatcctcg tccgaaaggt aacgaccgat tacggtcaga caaaagcgaa tgtaaaggat
     3901 taccagatcg cgaagttgga attgttctca gccttcaaga gggaggatct tggatcatgg
     3961 ctaaagaaac caatagagca ggacgagttt ggattggctg aatgactgca gagctttcgt
     4021 tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac
     4081 accagaatcc tactgagttc gagtattatg gcattgggaa acatgttttt cttgtaccat
     4141 ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg
     4201 gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata
     4261 ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aaatataaga gatatgcaaa
     4321 cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga
     4381 ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc
     4441 agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat
     4501 ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta
     4561 taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt
     4621 atgacttgcc aattgattga caacatgcat caagcttggc actggccgtc gttttacaac
     4681 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     4741 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4801 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc
     4861 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     4921 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     4981 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     5041 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     5101 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     5161 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     5221 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     5281 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     5341 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     5401 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     5461 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     5521 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     5581 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     5641 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     5701 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     5761 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     5821 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     5881 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     5941 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     6001 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     6061 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     6121 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     6181 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     6241 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     6301 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     6361 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     6421 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     6481 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     6541 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     6601 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     6661 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     6721 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     6781 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     6841 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     6901 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     6961 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     7021 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     7081 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     7141 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     7201 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     7261 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     7321 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     7381 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     7441 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     7501 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     7561 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     7621 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     7681 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     7741 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     7801 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     7861 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     7921 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     7981 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     8041 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     8101 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     8161 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     8221 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     8281 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     8341 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     8401 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     8461 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     8521 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     8581 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     8641 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     8701 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     8761 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     8821 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8881 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     8941 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     9001 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     9061 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9661 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9901 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     9961 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    10021 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    10081 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    10141 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    10201 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    10261 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    10321 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    10381 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    10441 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    10501 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    10561 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    10621 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    10681 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    10741 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    10801 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    10861 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    10921 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    10981 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    11041 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    11101 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    11161 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
    11221 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
    11281 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
    11341 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
    11401 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg
    11461 gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
    11521 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
    11581 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
    11641 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
    11701 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
    11761 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
    11821 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
    11881 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
    11941 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
    12001 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
    12061 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
    12121 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
    12181 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
    12241 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
    12301 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
    12361 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
    12421 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
    12481 tgagttcagg ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgagagagat
    12541 agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
    12601 tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
    12661 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    12721 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
    12781 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
    12841 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
    12901 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
    12961 agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg
    13021 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    13081 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    13141 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
    13201 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
    13261 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg
    13321 caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    13381 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    13441 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    13501 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattac
//
