LOCUS       Exported               13607 bp ds-DNA     circular SYN 15-MAY-2023
DEFINITION  Plant expression vector pER8, complete plasmid sequence.
ACCESSION   AF309825
VERSION     .
KEYWORDS    pZG161-X
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Zuo J, Niu QW, Chua NH.
  TITLE     Technical advance: An estrogen receptor-based transactivator XVE 
            mediates highly inducible gene expression in transgenic plants
  JOURNAL   Plant J. 24 (2), 265-273 (2000)
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Zuo J, Chua N-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2000) Laboratory of Plant Molecular Biology, The 
            Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
REFERENCE   3  (bases 1 to 13607)
  AUTHORS   Zuo J, Chua N-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2000) Laboratory of Plant Molecular Biology, The 
            Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
REFERENCE   4  (bases 1 to 13607)
  AUTHORS   nkxugy07
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     On Dec 22, 2000 this sequence version replaced gi:11527333.
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     promoter        33..275
                     /label=G10-90 promoter
                     /note="G10-90 promoter"
                     /note="color: #ffffff"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             291..1742
                     /codon_start=1
                     /transl_table=11
                     /product="XVE fusion protein"
                     /label=XVE fusion protein
                     /note="color: #993366"
                     /db_xref="GI:11527334"
                     /protein_id="AAG36984.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPSSAPPTDVSLGDELHLD
                     GEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGID
                     EYGGDPSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPT
                     RPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVW
                     RSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSII
                     LLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLIL
                     SHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLAT
                     AGSTSSHSLQKYYITGEAEGFPATV"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /note="color: #993366"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /note="color: #993366"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /note="color: #993366"
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      1882..2176
                     /label=pea rbcS E9 Ter
                     /note="pea rbcS E9"
                     /note="color: #ffffff"
     promoter        2192..2561
                     /label=NOS Promoter
                     /note="Nos"
                     /note="color: #ffffff"
     promoter        2294..2474
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        2327..2510
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2562..3143
                     /codon_start=1
                     /label=basta
                     /note="color: #cc99ff"
                     /translation="MKKPELTATSMSPERRPADIRRATEADMPAVCTIVNHYIETSTVN
                     FRTEPQEPQEWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVS
                     PRHQRTGLGSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGF
                     KHGNWHDVGFWQLDFSLPVPPRPVLPVTEI"
     CDS             2592..3143
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /note="color: #ccffcc"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3145..3397
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     terminator      3146..3421
                     /label=NOS Ter
                     /note="color: #ffffff"
     misc_feature    3432..3702
                     /label=LexA operator
                     /note="LexA operator"
                     /note="color: #a6acb3"
     promoter        3720..3766
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3724..3782
                     /label=35S Mini
                     /note="-46 to +12 of 35S promoter"
                     /note="color: #ffffff"
     CDS             3801..5009
                     /codon_start=1
                     /label=dADARcd
                     /note="color: #993366"
                     /translation="MYSPMVVPQKNVPLPIDDKSSSMELPQIHADTIGRLVLEKFMEVI
                     KGQEAYSRRKVLAGIVMTENMNFCEAKVISVSTGTKCVSGEHMSVNGAVLNDSHAEIVS
                     RRCLLKYLYAQLDLQCNQATAYQSIFVRNTDGQYPYKLKSGVHFHLYINTAPCGDARIF
                     SPHENDTGVDKHPNRKARGQLRTKIESGQGTIPVKSSDGIQTWDGVLQGQRLLTMSCSD
                     KIARWNIVGIQGSLLSSIIEPVYLHSIVLGSLLHPEHMYRAVCGRIEKSIQGLPPPYHL
                     NKPRLALVTSAEPRNQAKAPNFGINWTIGDTELEVVNSLTGRTIGGQVSRITKQAFFVK
                     YGFLMANLPGILVRKVTTDYGQTKANVKDYQIAKLELFSAFKREDLGSWLKKPIEQDEF
                     GLAE"
     misc_feature    5023..5036
                     /label=LIC1
                     /note="color: #a6acb3"
     misc_feature    5041..5054
                     /label=LIC2
                     /note="color: #a6acb3"
     misc_feature    5062..5106
                     /label=Linker
                     /note="color: #99ccff"
     CDS             5113..6321
                     /codon_start=1
                     /label=dADARcd
                     /note="color: #993366"
                     /translation="YSPMVVPQKNVPLPIDDKSSSMELPQIHADTIGRLVLEKFMEVIK
                     GQEAYSRRKVLAGIVMTENMNFCEAKVISVSTGTKCVSGEHMSVNGAVLNDSHAEIVSR
                     RCLLKYLYAQLDLQCNQATAYQSIFVRNTDGQYPYKLKSGVHFHLYINTAPCGDARIFS
                     PHENDTGVDKHPNRKARGQLRTKIESGQGTIPVKSSDGIQTWDGVLQGQRLLTMSCSDK
                     IARWNIVGIQGSLLSSIIEPVYLHSIVLGSLLHPEHMYRAVCGRIEKSIQGLPPPYHLN
                     KPRLALVTSAEPRNQAKAPNFGINWTIGDTELEVVNSLTGRTIGGQVSRITKQAFFVKY
                     GFLMANLPGILVRKVTTDYGQTKANVKDYQIAKLELFSAFKREDLGSWLKKPIEQDEFG
                     LAE"
     terminator      6333..6802
                     /label=pea 3A Ter
                     /note="pea 3A"
                     /note="color: #ffffff"
     primer_bind     complement(6835..6851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    7072..7097
                     /label=LB
                     /note="color: #a6acb3"
     misc_feature    7073..7097
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             7625..8416
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8660..9248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    9434..9574
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      9918..10112
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     CDS             complement(10178..11245)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(11679..12308)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    join(13588..13607,1..6)
                     /label=RB
                     /note="color: #a6acb3"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcgaattc ccagagatgt tagctgaaat catcactaat cagataccaa
     1801 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg
     1861 agtaagctag cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
     1921 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
     1981 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
     2041 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
     2101 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
     2161 gtaaaacatc ccaaacaagc ttggaaactg aaggcgggaa acgacaatct gatcatgagc
     2221 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
     2281 tttggaactg acagaaccgc aacgattgaa ggagccactc agccgcgggt ttctggagtt
     2341 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     2401 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     2461 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatccgc
     2521 tagaggatct cgacctgcaa gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
     2581 ccgcgacgtc tatgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
     2641 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
     2701 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
     2761 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggaccct
     2821 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
     2881 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
     2941 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
     3001 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
     3061 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
     3121 tcctgcccgt caccgagatc taacgatcgt tcaaacattt ggcaataaag tttcttaaga
     3181 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     3241 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     3301 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     3361 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg gaattgatcc cccctcgaca
     3421 gcttgcatgc cagcttgggc tgcaggtcga ggctaaaaaa ctaatcgcat tatcatcccc
     3481 tcgacgtact gtacatataa ccactggttt tatatacagc agtactgtac atataaccac
     3541 tggttttata tacagcagtc gacgtactgt acatataacc actggtttta tatacagcag
     3601 tactgtacat ataaccactg gttttatata cagcagtcga ggtaagatta gatatggata
     3661 tgtatatgga tatgtatatg gtggtaatgc catgtaatat gctcgactct aggatcttcg
     3721 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctgaagctag
     3781 tcgactctag cctcgagacc atgtactctc ctatggtcgt accgcaaaaa aatgtgcctc
     3841 tccctataga cgataaatct agctccatgg agcttccgca gatccacgca gacactatcg
     3901 gtcgattggt gctggaaaag ttcatggagg ttattaaagg tcaggaggct tatagccgtc
     3961 gaaaagtttt ggctggaatc gtaatgaccg aaaacatgaa cttctgtgag gcaaaagtca
     4021 tttcagtgtc cacgggcacc aagtgtgtgt ccggcgagca tatgtccgta aacggtgcgg
     4081 tactcaatga cagccacgcc gagatagtgt cacgtcgttg tctcctcaag tatctctatg
     4141 cccaactgga tcttcaatgc aaccaggcga ccgcttatca gagcatattt gtgcgtaata
     4201 ctgacggtca gtatccctat aaactgaagt ctggggtcca ttttcatctc tatataaaca
     4261 cggcgccctg cggcgatgcc cgtatttttt cacctcacga gaatgacacg ggagtagata
     4321 aacatcccaa ccgaaaagca agagggcagc tccgaaccaa aatcgaatcc ggccagggca
     4381 caatcccagt gaaatctagc gacggcattc aaacttggga cggcgtacta caaggccagc
     4441 gtttgcttac gatgtcctgc tccgataaaa ttgctaggtg gaacatcgtc ggtatccagg
     4501 gctccttgct ctcttcaatt atagagcccg tctatctgca ttcaattgtc ttagggtcac
     4561 tcttgcatcc tgaacatatg taccgagccg tttgtgggag aatagaaaag agtatccagg
     4621 gtctaccccc accttaccat ttgaacaaac ccagactggc attggtcaca tccgcagaac
     4681 cacgtaacca agccaaggcc ccgaattttg gaataaactg gactataggt gacacggaac
     4741 tagaggttgt gaactctctc acaggcagaa ccataggagg gcaagtcagc cgtataacga
     4801 agcaggcttt tttcgtaaag tatggtttct tgatggctaa tttgcccgga atcctcgtcc
     4861 gaaaggtaac gaccgattac ggtcagacaa aagcgaatgt aaaggattac cagatcgcga
     4921 agttggaatt gttctcagcc ttcaagaggg aggatcttgg atcatggcta aagaaaccaa
     4981 tagagcagga cgagtttgga ttggctgaag ctcgagtacc ttcgacgaca agaccgggcc
     5041 cggctcttct cctcactcga ccagatctcg tacgcgtccc ggggcggtgg ctcatctggc
     5101 ggaggtggat cctactctcc tatggtcgta ccgcaaaaaa atgtgcctct ccctatagac
     5161 gataaatcta gctccatgga gcttccgcag atccacgcag acactatcgg tcgattggtg
     5221 ctggaaaagt tcatggaggt tattaaaggt caggaggctt atagccgtcg aaaagttttg
     5281 gctggaatcg taatgaccga aaacatgaac ttctgtgagg caaaagtcat ttcagtgtcc
     5341 acgggcacca agtgtgtgtc cggcgagcat atgtccgtaa acggtgcggt actcaatgac
     5401 agccacgccg agatagtgtc acgtcgttgt ctcctcaagt atctctatgc ccaactggat
     5461 cttcaatgca accaggcgac cgcttatcag agcatatttg tgcgtaatac tgacggtcag
     5521 tatccctata aactgaagtc tggggtccat tttcatctct atataaacac ggcgccctgc
     5581 ggcgatgccc gtattttttc acctcacgag aatgacacgg gagtagataa acatcccaac
     5641 cgaaaagcaa gagggcagct ccgaaccaaa atcgaatccg gccagggcac aatcccagtg
     5701 aaatctagcg acggcattca aacttgggac ggcgtactac aaggccagcg tttgcttacg
     5761 atgtcctgct ccgataaaat tgctaggtgg aacatcgtcg gtatccaggg ctccttgctc
     5821 tcttcaatta tagagcccgt ctatctgcat tcaattgtct tagggtcact cttgcatcct
     5881 gaacatatgt accgagccgt ttgtgggaga atagaaaaga gtatccaggg tctaccccca
     5941 ccttaccatt tgaacaaacc cagactggca ttggtcacat ccgcagaacc acgtaaccaa
     6001 gccaaggccc cgaattttgg aataaactgg actataggtg acacggaact agaggttgtg
     6061 aactctctca caggcagaac cataggaggg caagtcagcc gtataacgaa gcaggctttt
     6121 ttcgtaaagt atggtttctt gatggctaat ttgcccggaa tcctcgtccg aaaggtaacg
     6181 accgattacg gtcagacaaa agcgaatgta aaggattacc agatcgcgaa gttggaattg
     6241 ttctcagcct tcaagaggga ggatcttgga tcatggctaa agaaaccaat agagcaggac
     6301 gagtttggat tggctgaatg aactagtcga tccaggcctc ccagctttcg tccgtatcat
     6361 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcccaca caccagaatc
     6421 ctactaagtt tgagtattat ggcattggaa aagctgtttt cttctatcat ttgttctgct
     6481 tgtaatttac tgtgttcttt cagtttttgt tttcggacat caaaatgcaa atggatggat
     6541 aagagttaat aaatgatatg gtccttttgt tcattctcaa attattatta tctgttgttt
     6601 ttactttaat gggttgaatt taagtaagaa aggaactaac agtgtgatat taaggtgcaa
     6661 tgttagacat ataaaacagt ctttcacctc tctttggtta tgtcttgaat tggtttgttt
     6721 cttcacttat ctgtgtaatc aagtttacta tgagtctatg atcaagtaat tatgcaatca
     6781 agttaagtac agtataggct ttttgtgtcg agggggtacc gagtcgagga attcactggc
     6841 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6901 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6961 ccaacagttg cgcagcctga atggcgggta ccgagctcga attcaattcg gcgttaattc
     7021 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     7081 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     7141 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     7201 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     7261 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     7321 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctcgtt cggttcgtaa
     7381 gctgtaatgc aagtagcgta actgccgtca cgcaactggt ccagaacctt gaccgaacgc
     7441 agcggtggta acggcgcagt ggcggttttc atggcttctt gttatgacat gtttttttgg
     7501 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     7561 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     7621 catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     7681 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     7741 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     7801 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     7861 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     7921 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     7981 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     8041 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     8101 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     8161 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     8221 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     8281 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     8341 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     8401 ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgccggcg
     8461 ttaactcaag cgattagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     8521 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     8581 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9301 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9361 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9421 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     9481 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     9541 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     9601 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     9661 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     9721 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     9781 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     9841 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     9901 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     9961 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
    10021 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
    10081 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
    10141 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
    10201 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
    10261 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
    10321 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
    10381 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
    10441 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
    10501 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
    10561 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
    10621 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
    10681 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
    10741 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
    10801 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
    10861 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
    10921 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
    10981 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
    11041 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
    11101 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
    11161 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
    11221 gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg
    11281 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    11341 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    11401 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    11461 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    11521 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    11581 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    11641 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    11701 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    11761 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    11821 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    11881 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    11941 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    12001 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    12061 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    12121 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    12181 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    12241 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    12301 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    12361 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    12421 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    12481 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    12541 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    12601 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    12661 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    12721 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    12781 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    12841 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    12901 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    12961 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    13021 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    13081 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    13141 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    13201 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    13261 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    13321 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    13381 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    13441 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    13501 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    13561 cgccctttta aatatccgtt attctaataa acgctctttt ctcttag
//
