LOCUS       Exported               12850 bp ds-DNA     circular SYN 15-MAY-2023
DEFINITION  Plant expression vector pER8, complete plasmid sequence.
ACCESSION   AF309825
VERSION     .
KEYWORDS    pER8-ApaI-dADARcd
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Zuo J, Niu QW, Chua NH.
  TITLE     Technical advance: An estrogen receptor-based transactivator XVE 
            mediates highly inducible gene expression in transgenic plants
  JOURNAL   Plant J. 24 (2), 265-273 (2000)
REFERENCE   2  (bases 1 to 12850)
  AUTHORS   Zuo J, Chua N-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2000) Laboratory of Plant Molecular Biology, The 
            Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
REFERENCE   3  (bases 1 to 12850)
  AUTHORS   Zuo J, Chua N-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2000) Laboratory of Plant Molecular Biology, The 
            Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
REFERENCE   4  (bases 1 to 12850)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     On Dec 22, 2000 this sequence version replaced gi:11527333.
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     promoter        33..275
                     /label=G10-90 promoter
                     /note="G10-90 promoter"
                     /note="color: #ffffff"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             291..1742
                     /codon_start=1
                     /transl_table=11
                     /product="XVE fusion protein"
                     /label=XVE fusion protein
                     /note="color: #993366"
                     /db_xref="GI:11527334"
                     /protein_id="AAG36984.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPSSAPPTDVSLGDELHLD
                     GEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGID
                     EYGGDPSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPT
                     RPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVW
                     RSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSII
                     LLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLIL
                     SHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLAT
                     AGSTSSHSLQKYYITGEAEGFPATV"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /note="color: #993366"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /note="color: #993366"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    828..1742
                     /label=ER (282-595)
                     /note="color: #a6acb3"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /note="color: #993366"
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     3'UTR           1762..1856
                     /label=rat glucocorticoid receptor 3'UTR_1
                     /note="rat glucocorticoid receptor"
                     /note="color: #a6acb3"
     terminator      1882..2176
                     /label=pea rbcS E9 terminator
                     /note="pea rbcS E9"
                     /note="color: #ffffff"
     promoter        2192..2561
                     /label=NOS Promoter
                     /note="Nos"
                     /note="color: #ffffff"
     promoter        2294..2474
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        2327..2510
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2519..2561
                     /label=extra bp difference of reported sequence
                     /note="color: #a6acb3"
     CDS             2562..3587
                     /codon_start=1
                     /label=hptII
                     /note="color: #99ccff"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3273..3296
                     /label=oWH22
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(3356..3379)
                     /label=oWH23
                     /note="color: #a020f0; direction: LEFT"
     terminator      3589..3841
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     terminator      3590..3865
                     /label=NOS
                     /note="color: #ffffff"
     misc_feature    3876..4146
                     /label=LexA operator
                     /note="LexA operator"
                     /note="color: #a6acb3"
     promoter        4164..4210
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4168..4226
                     /label=-46 to +12 of 35S minimal promoter
                     /note="-46 to +12 of 35S promoter"
                     /note="color: #ffffff"
     CDS             4251..5459
                     /codon_start=1
                     /label=dADARcd
                     /note="color: #993366"
                     /translation="MYSPMVVPQKNVPLPIDDKSSSMELPQIHADTIGRLVLEKFMEVI
                     KGQEAYSRRKVLAGIVMTENMNFCEAKVISVSTGTKCVSGEHMSVNGAVLNDSHAEIVS
                     RRCLLKYLYAQLDLQCNQATAYQSIFVRNTDGQYPYKLKSGVHFHLYINTAPCGDARIF
                     SPHENDTGVDKHPNRKARGQLRTKIESGQGTIPVKSSDGIQTWDGVLQGQRLLTMSCSD
                     KIARWNIVGIQGSLLSSIIEPVYLHSIVLGSLLHPEHMYRAVCGRIEKSIQGLPPPYHL
                     NKPRLALVTSAEPRNQAKAPNFGINWTIGDTELEVVNSLTGRTIGGQVSRITKQAFFVK
                     YGFLMANLPGILVRKVTTDYGQTKANVKDYQIAKLELFSAFKREDLGSWLKKPIEQDEF
                     GLAE"
     primer_bind     4895..4917
                     /label=oZG520
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(5020..5042)
                     /label=OZG521
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    5466..5513
                     /label=Linker
                     /note="color: #99ccff"
     misc_feature    5522..5535
                     /label=LIC1
                     /note="color: #c0c0c0"
     misc_feature    5540..5553
                     /label=LIC2
                     /note="color: #c0c0c0"
     terminator      5576..6045
                     /label=pea 3A terminator
                     /note="pea 3A"
                     /note="color: #ffffff"
     primer_bind     complement(6078..6094)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    6315..6340
                     /label=LB
                     /note="color: #a6acb3"
     misc_feature    6316..6340
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             6868..7659
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7903..8491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    8677..8817
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      9161..9355
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     CDS             complement(9421..10488)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10922..11551)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    join(12831..12850,1..6)
                     /label=RB
                     /note="color: #a6acb3"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcgaattc ccagagatgt tagctgaaat catcactaat cagataccaa
     1801 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg
     1861 agtaagctag cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
     1921 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
     1981 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
     2041 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
     2101 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
     2161 gtaaaacatc ccaaacaagc ttggaaactg aaggcgggaa acgacaatct gatcatgagc
     2221 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
     2281 tttggaactg acagaaccgc aacgattgaa ggagccactc agccgcgggt ttctggagtt
     2341 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     2401 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     2461 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatccgc
     2521 tagaggatct cgacctgcaa gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
     2581 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     2641 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     2701 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     2761 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     2821 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     2881 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     2941 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3001 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3061 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3121 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3181 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3241 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3301 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     3361 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     3421 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     3481 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     3541 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcga tcgttcaaac
     3601 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3661 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3721 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3781 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3841 cggggaattg atcccccctc gacagcttgc atgccagctt gggctgcagg tcgaggctaa
     3901 aaaactaatc gcattatcat cccctcgacg tactgtacat ataaccactg gttttatata
     3961 cagcagtact gtacatataa ccactggttt tatatacagc agtcgacgta ctgtacatat
     4021 aaccactggt tttatataca gcagtactgt acatataacc actggtttta tatacagcag
     4081 tcgaggtaag attagatatg gatatgtata tggatatgta tatggtggta atgccatgta
     4141 atatgctcga ctctaggatc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
     4201 ttggagagga cacgctgaag ctagtcgact ctagcctcga ggagctcacc atgtactctc
     4261 ctatggtcgt accgcaaaaa aatgtgcctc tccctataga cgataaatct agctccatgg
     4321 agcttccgca gatccacgca gacactatcg gtcgattggt gctggaaaag ttcatggagg
     4381 ttattaaagg tcaggaggct tatagccgtc gaaaagtttt ggctggaatc gtaatgaccg
     4441 aaaacatgaa cttctgtgag gcaaaagtca tttcagtgtc cacgggcacc aagtgtgtgt
     4501 ccggcgagca tatgtccgta aacggtgcgg tactcaatga cagccacgcc gagatagtgt
     4561 cacgtcgttg tctcctcaag tatctctatg cccaactgga tcttcaatgc aaccaggcga
     4621 ccgcttatca gagcatattt gtgcgtaata ctgacggtca gtatccctat aaactgaagt
     4681 ctggggtcca ttttcatctc tatataaaca cggcgccctg cggcgatgcc cgtatttttt
     4741 cacctcacga gaatgacacg ggagtagata aacatcccaa ccgaaaagca agagggcagc
     4801 tccgaaccaa aatcgaatcc ggccagggca caatcccagt gaaatctagc gacggcattc
     4861 aaacttggga cggcgtacta caaggccagc gtttgcttac gatgtcctgc tccgataaaa
     4921 ttgctaggtg gaacatcgtc ggtatccagg gctccttgct ctcttcaatt atagagcccg
     4981 tctatctgca ttcaattgtc ttagggtcac tcttgcatcc tgaacatatg taccgagccg
     5041 tttgtgggag aatagaaaag agtatccagg gtctaccccc accttaccat ttgaacaaac
     5101 ccagactggc attggtcaca tccgcagaac cacgtaacca agccaaggcc ccgaattttg
     5161 gaataaactg gactataggt gacacggaac tagaggttgt gaactctctc acaggcagaa
     5221 ccataggagg gcaagtcagc cgtataacga agcaggcttt tttcgtaaag tatggtttct
     5281 tgatggctaa tttgcccgga atcctcgtcc gaaaggtaac gaccgattac ggtcagacaa
     5341 aagcgaatgt aaaggattac cagatcgcga agttggaatt gttctcagcc ttcaagaggg
     5401 aggatcttgg atcatggcta aagaaaccaa tagagcagga cgagtttgga ttggctgaat
     5461 ctagaggagg tggctcatct ggcggaggtc agatctcgta cgcgtcccgg ggcggtacct
     5521 tcgacgacaa gaccgggccc ggctcttctc ctcactcgac ctgcactagt cgatccaggc
     5581 ctcccagctt tcgtccgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
     5641 ttcattgccc acacaccaga atcctactaa gtttgagtat tatggcattg gaaaagctgt
     5701 tttcttctat catttgttct gcttgtaatt tactgtgttc tttcagtttt tgttttcgga
     5761 catcaaaatg caaatggatg gataagagtt aataaatgat atggtccttt tgttcattct
     5821 caaattatta ttatctgttg tttttacttt aatgggttga atttaagtaa gaaaggaact
     5881 aacagtgtga tattaaggtg caatgttaga catataaaac agtctttcac ctctctttgg
     5941 ttatgtcttg aattggtttg tttcttcact tatctgtgta atcaagttta ctatgagtct
     6001 atgatcaagt aattatgcaa tcaagttaag tacagtatag gctttttgtg tcgagggggt
     6061 accgagtcga ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgg gtaccgagct
     6241 cgaattcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     6301 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     6361 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     6421 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     6481 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     6541 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     6601 cataagcctc gttcggttcg taagctgtaa tgcaagtagc gtaactgccg tcacgcaact
     6661 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     6721 cttgttatga catgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     6781 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     6841 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     6901 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     6961 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     7021 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     7081 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     7141 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     7201 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     7261 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     7321 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     7381 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     7441 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     7501 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     7561 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     7621 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     7681 aagccgacgc cgcttcgccg gcgttaactc aagcgattag atgcactaag cacataattg
     7741 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     7801 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     7861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8041 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8581 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8641 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     8701 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     8761 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     8821 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8881 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8941 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     9001 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     9061 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     9121 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     9181 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     9241 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     9301 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     9361 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     9421 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     9481 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     9541 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     9601 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     9661 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     9721 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     9781 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     9841 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     9901 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     9961 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
    10021 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
    10081 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
    10141 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
    10201 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
    10261 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
    10321 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
    10381 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
    10441 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgt cacgccgatt
    10501 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
    10561 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    10621 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    10681 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    10741 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    10801 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    10861 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    10921 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    10981 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    11041 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    11101 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    11161 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    11221 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    11281 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    11341 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    11401 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    11461 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    11521 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    11581 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    11641 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    11701 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    11761 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    11821 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    11881 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    11941 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    12001 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    12061 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    12121 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    12181 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    12241 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    12301 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    12361 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    12421 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    12481 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    12541 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    12601 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    12661 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    12721 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    12781 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gttattctaa taaacgctct
    12841 tttctcttag
//
