LOCUS       pJB37                   6522 bp ds-DNA     circular     14-MAY-2023
DEFINITION  .
KEYWORDS    "marker:SmR"
COMMENT     synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..6522
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: other DNA: organism: synthetic DNA construct"
     CDS             complement(248..1039)
                     /label="SmR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: aadA label: SmR note: confers resistance to spectinomycin and streptomycin product: aminoglycoside adenylyltransferase (Murphy, 1985) transl_table: 1 translation: MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     oriT            1517..1841
                     /label="oriT (F)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(2366..3316)
                     /label="Rep101"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: rep101 label: Rep101 product: RepA protein needed for replication with the pSC101 origin transl_table: 1 translation: MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
     rep_origin      3364..3586
                     /label="pSC101 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: pSC101 ori note: low-copy replication origin that requires the Rep101 protein"
     CDS             complement(4322..5200)
                     /label="araC"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: araC label: araC product: L-arabinose regulatory protein transl_table: 1 translation: MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        5227..5511
                     /label="araBAD promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: araBAD label: araBAD promoter note: promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)"
     gene            5554..6516
                     /label="RcaT-Sen2"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
ORIGIN
        1 agtaaagccc tcgctagatt ttaatgcgga tgttgcgatt acttcgccaa ctattgcgat
       61 aacaagaaaa agccagcctt tcatgatata tctcccaatt tgtgtagggc ttattatgca
      121 cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct
      181 tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattg
      241 ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
      301 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
      361 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
      421 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
      481 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
      541 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
      601 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
      661 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
      721 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
      781 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
      841 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
      901 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
      961 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     1021 cgatcaccgc ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
     1081 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     1141 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
     1201 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     1261 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
     1321 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     1381 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
     1441 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
     1501 gaagacctcg gccgccacct ctggtgactt tatccgtaaa taatttaacc cactccacaa
     1561 aaaggctcaa caggttggtg gttctcacca ccaaaagcac cacaccccac gcaaaaacaa
     1621 gtttttgctg atttttcttt ataaatagag tgttatgaaa aattagtttc tcttactctc
     1681 tttatgatat ttaaaaaagc ggtgtcggcg cggctacaac aacgcgccga caccgttttg
     1741 taggggtggt actgactatt tttataaaaa acattatttt atattagggg tgctgctagc
     1801 ggcgcggtgt gtttttttat aggataccgc taggggcgct gcattaattc ccacgggttt
     1861 tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc gcagatccgg
     1921 cttcaggttt gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac
     1981 gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg
     2041 tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc
     2101 atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc
     2161 atctgttaca ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa
     2221 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc
     2281 ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact
     2341 gatagataca agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta
     2401 gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt
     2461 gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat
     2521 cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta
     2581 ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc
     2641 tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca
     2701 atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa
     2761 gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc
     2821 taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat
     2881 aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga
     2941 atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt
     3001 cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat
     3061 tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag
     3121 cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc
     3181 gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta
     3241 gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa
     3301 caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga
     3361 gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat
     3421 tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta
     3481 gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag
     3541 aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag
     3601 ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt
     3661 aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt
     3721 ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg
     3781 ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat
     3841 gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg
     3901 gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat
     3961 tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc
     4021 cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtcgggaa ttcaattgat
     4081 atttgcttgc tcttccccat attttcctgc ttaccgccat tcagctggta gtacctgtcg
     4141 caaattcttt acagttttta aactaatgag acgaatctga tcgacgcaaa aagtccgtat
     4201 gcctactatt agctcacggt tataaactcg aggcataatg tgcctgtcaa atggacgaag
     4261 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca
     4321 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
     4381 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
     4441 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
     4501 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
     4561 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
     4621 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg
     4681 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
     4741 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
     4801 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa
     4861 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
     4921 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga
     4981 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct
     5041 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
     5101 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
     5161 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
     5221 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
     5281 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
     5341 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
     5401 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
     5461 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
     5521 tttgggctag cgaattcgag ctcggtaccc gggatgtttg aagaaactat taaaaaacaa
     5581 tttgaattgt tagacataag taacttcaat gttgatatat ctcatcgtct ccttttcgtg
     5641 tgtggtggca aagtcgatgt acgtgcacca attcccccca gttttaggga tagactactc
     5701 acttacacag caaaaaatgc ctctgaactt cacgaacatt tcattctagc tgagacattc
     5761 aaggattatt ttaaagagaa tgcctatccg gaccttctgg tgtttgaaga tgatatagcc
     5821 agtatttcat ctcttattat cattttttta gaaagcccag gatcattagt tgagttaggt
     5881 atattttgca acaaatcaga actatttaaa aaaatactga ttgttgcttc agcggaagaa
     5941 gtatacggtg aggactcatt catttattta ggcccactag agtatattaa gaagaaagtc
     6001 tcatcctctg ttgtaatcta tccttggccg gatcctgaag tcttaaaata cgataatgat
     6061 tttcttgatg atttatgtgt aaatataaaa gaaaaacttt catcaatccc aaaaacagaa
     6121 caattcagta aggataattc tggacatata gcacttctaa ttactgaaat tatatctctt
     6181 tgcgccccaa tacaacttag tgaaatagaa tcagccctaa actcattagg gattaatatt
     6241 tcaacaaaaa ttattaacag atcaatttat ttacttcaga aagttggttt tatagacgtt
     6301 ttatcatata gctcaaacaa atattacttt ccattaaaag agcgcaaatg ggtaaaattt
     6361 ggtaaaacta aagataataa actcatagat aatcaacaat taaaaatgaa ggttagacaa
     6421 tcttttgtaa cattaaccga tccattatca aaacgaagaa ttacagcgct gcgtcaaata
     6481 atagcaaaaa aagaaatggc tgaggagatt aattaaaagc tt
//