LOCUS       pcDNA3.1/Puro-CAG-JEDI- 8212 bp ds-DNA     circular     16-MAY-2023
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...4ko-3ky3ahq (modified)
VERSION     urn.local...4ko-3ky3ahq
COMMENT     Imported using the Genbank importer. File name:
            pCaggs-JEDI-1P-p2a-mCherry-Caax.gb
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label="SalI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Recognition_pattern: G^TCGAC annotation_group: GTCGAC note: Geneious type: restriction site"
     misc_feature    23..356
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created_by: User"
     misc_feature    252..257
                     /label="NdeI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Recognition_pattern: CA^TATG annotation_group: CATATG note: Geneious type: restriction site"
     misc_feature    357..362
                     /label="Eco105I site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created_by: User"
     misc_feature    357..411
                     /label="Appears part of CMV enhancer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="created_by: User"
     promoter        363..1723
                     /label="Actin promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created_by: User"
     misc_feature    1628..1633
                     /label="XbaI"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Recognition_pattern: T^CTAGA annotation_group: TCTAGA note: Geneious type: restriction site"
     misc_feature    1724..1729
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Recognition_pattern: G^AATTC annotation_group: GAATTC note: Geneious type: restriction site"
     misc_feature    1730..1735
                     /label="XhoI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Recognition_pattern: C^TCGAG annotation_group: CTCGAG note: Geneious type: restriction site"
     misc_feature    1736..1741
                     /label="EcoRI"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Recognition_pattern: G^AATTC annotation_group: GAATTC note: Geneious type: restriction site"
     misc_feature    1816..1821
                     /label="BglII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Recognition_pattern: A^GATCT annotation_group: AGATCT note: Geneious type: restriction site"
     misc_feature    1828..1833
                     /label="NheI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Recognition_pattern: G^CTAGC annotation_group: GCTAGC note: Geneious type: restriction site"
     misc_feature    1834..1840
                     /label="Kozak"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="created_by: User"
     CDS             1841..3121
                     /label="JEDI-1P"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="created_by: User Transferred_From: pCS2-Hygro-ChickenVSD(R154Q)-Mermaid(partial) Transferred_Similarity: 100.00% modified_by: User modified_by: User modified_by: User"
     CDS             1841..3121
                     /label="JEDI-1P"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User Transferred_From: pCS2-Hygro-ChickenVSD(R154Q)-Mermaid(partial) Transferred_Similarity: 100.00% modified_by: User modified_by: User modified_by: User"
                     /translation="METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQDSCSNTIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFFLVDVLMRVFVEGFKNYFRSKLVTLDAVIVVGTLLINMTYSFSDLAAFNSENVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNTDQMPHMVTLLRVLKIVILIRIFRLASQKKQLEVVT"
     gene            2285..3013
                     /label="cpsfGFP-OPT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created_by: User modified_by: User modified_by: User"
     misc_feature    2810..2815
                     /label="NdeI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Recognition_pattern: CA^TATG annotation_group: CATATG note: Geneious type: restriction site"
     misc_feature    3122..3127
                     /label="BamHI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Recognition_pattern: G^GATCC annotation_group: GGATCC note: Geneious type: restriction site"
     misc_feature    3122..3187
                     /label="P2A"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="created_by: User modified_by: User"
     CDS             3185..3187
                     /label="MacQ-mRuby3"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="created_by: User modified_by: User"
     CDS             3185..3187
                     /label="MacQ-mRuby3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User modified_by: User"
                     /translation="P"
     CDS             3188..3961
                     /label="mCherry-CaaX"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="created_by: User"
     CDS             3188..3961
                     /label="mCherry-CaaX"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKLKKMSKDGKKKKKKSKTKCVIM"
     misc_feature    3539..3544
                     /label="PstI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Recognition_pattern: CTGCA^G annotation_group: CTGCAG note: Geneious type: restriction site"
     misc_feature    3888..3893
                     /label="Bsp1407I"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Recognition_pattern: T^GTACA annotation_group: TGTACA note: Geneious type: restriction site"
     misc_feature    3893..3898
                     /label="HindIII"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Recognition_pattern: A^AGCTT annotation_group: AAGCTT note: Geneious type: restriction site"
     misc_feature    3896..3901
                     /label="Afl2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: green"
     misc_feature    3902..3961
                     /label="CAAX"
                     /ApEinfo_revcolor="#cb10c9"
                     /ApEinfo_fwdcolor="#cb10c9"
                     /note="ApEinfo_revcolor: green modified_by: User"
     misc_feature    3964..3965
                     /label="TGA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Original_Bases: TGA note: Geneious type: Editing History Deletion"
     misc_feature    3965..3970
                     /label="HindIII"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Recognition_pattern: A^AGCTT annotation_group: AAGCTT note: Geneious type: restriction site"
     misc_feature    3971..3976
                     /label="KpnI"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Recognition_pattern: GGTAC^C annotation_group: GGTACC note: Geneious type: restriction site"
     misc_feature    3983..3988
                     /label="BamHI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Recognition_pattern: G^GATCC annotation_group: GGATCC note: Geneious type: restriction site"
     misc_feature    4006..4011
                     /label="EcoRI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Recognition_pattern: G^AATTC annotation_group: GAATTC note: Geneious type: restriction site"
     misc_feature    4011..4016
                     /label="PstI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Recognition_pattern: CTGCA^G annotation_group: CTGCAG note: Geneious type: restriction site"
     misc_feature    4016..4021
                     /label="EcoRV, Eco32I"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Recognition_pattern: GAT^ATC annotation_group: GATATC note: Geneious type: restriction site"
     misc_feature    4016..4021
                     /label="EcoRV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Recognition_pattern: GAT^ATC annotation_group: GATATC note: Geneious type: restriction site"
     misc_feature    4032..4039
                     /label="NotI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Recognition_pattern: GC^GGCCGC annotation_group: GCGGCCGC note: Geneious type: restriction site"
     misc_feature    4039..4044
                     /label="XhoI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Recognition_pattern: C^TCGAG annotation_group: CTCGAG note: Geneious type: restriction site"
     misc_feature    4045..4050
                     /label="XbaI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Recognition_pattern: T^CTAGA annotation_group: TCTAGA note: Geneious type: restriction site"
     terminator      4079..4306
                     /label="bGH PA terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="created_by: User"
     rep_origin      4369..4675
                     /label="f1 origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created_by: User modified_by: User"
     misc_feature    complement(4795..5010)
                     /label="SV40 enhancer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="created_by: User"
     promoter        4807..5075
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User modified_by: User"
     rep_origin      4974..5051
                     /label="SV40 origin"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="created_by: User"
     CDS             5132..5728
                     /label="Puromycin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created_by: User modified_by: User"
     CDS             5132..5728
                     /label="Puromycin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User modified_by: User"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5181..5186
                     /label="SmaI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Recognition_pattern: CCC^GGG annotation_group: CCCGGG note: Geneious type: restriction site"
     misc_feature    5240..5245
                     /label="SalI"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Recognition_pattern: G^TCGAC annotation_group: GTCGAC note: Geneious type: restriction site"
     terminator      5894..6013
                     /label="SV40 PA terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created_by: User"
     misc_feature    6028..6033
                     /label="SalI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Recognition_pattern: G^TCGAC annotation_group: GTCGAC note: Geneious type: restriction site"
     promoter        complement(6110..6139)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="created_by: User"
     rep_origin      complement(6448..7064)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="created_by: User"
     CDS             complement(7219..8079)
                     /label="Ampicillin CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created_by: User"
     CDS             complement(7219..8079)
                     /label="Ampicillin CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(8121..8149)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created_by: User"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc tcgaggaatt
     1741 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     1801 tcacaaatac cactgagatc tgcatgagct agccgccacc atggagacga ctgtgaggta
     1861 tgaacagggg tcagagctca ctaaaacttc gagctctcca acagcagatg agcccacgat
     1921 aaagattgat gatggtcgtg atgagggtaa tgaacaagac agctgttcca ataccattag
     1981 gagaaaaatt tccccgtttg tgatgtcatt tggattcaga gtatttggag ttgtgcttat
     2041 cattgtagac atcatagtgg tgattgtgga tctggccatc agtgagaaga aaagaggcat
     2101 tagagagatt cttgaaggtg tttccctggc tatagcactc ttcttccttg ttgatgttct
     2161 catgagagtg tttgttgaag gcttcaagaa ctatttccgg tccaaactgg ttactttgga
     2221 tgcagtcata gtagtgggca ctctgctaat taatatgacc tactccttct ctgaccttgc
     2281 tgcctttaac agcgagaacg tgtatattac cgcggataaa cagaaaaacg gcattaaagc
     2341 gaactttacc gtgcgccata acgtggaaga tggcagcgtg cagctggcgg atcattatca
     2401 gcagaacacc ccgattggcg atggcccggt gctgctgccg gataaccatt atctgagcac
     2461 ccagaccgtg ctgagcaaag atccgaacga aaaacgcgat cacatggtgc tgctggaatt
     2521 tgtgaccgca gcgggcatta cacacggcat ggatgaactg tatggcggca ccggcggcag
     2581 cgcgagccag ggcgaagaac tgtttaccgg cgtggtgccg attctggtgg aactggatgg
     2641 cgatgtgaac ggccataaat ttagcgtgcg cggcgaaggc gaaggcgatg cgaccattgg
     2701 caaactgacc ctgaaattta tttgcaccac cggcaaacta ccggtgccgt ggccgaccct
     2761 ggtgaccacc ttaacctatg gcgtgcagtg ctttagccgc tatccggatc atatgaaacg
     2821 ccatgatttt tttaaaagcg cgatgccgga aggctatgtg caggaacgca ccattagctt
     2881 taaagatgat ggcaaatata aaacccgcgc ggtggtgaaa tttgaaggcg ataccctggt
     2941 gaaccgcatt gaactgaaag gcaccgattt taaagaagat ggcaacattc tggggcataa
     3001 actggaatat aacacagatc agatgccgca catggttact cttcttcgag ttctgaaaat
     3061 tgttatctta ataagaatat ttcgcctggc ttcacagaag aaacaacttg aagtggtaac
     3121 cggatccgga gccaccaact tcagcctgct gaagcaggca ggcgacgtgg aagagaaccc
     3181 tggccctatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     3241 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3301 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     3361 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     3421 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     3481 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     3541 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     3601 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     3661 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     3721 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     3781 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     3841 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagcttaa
     3901 gaagatgagc aaggacggca agaagaaaaa gaagaagtcc aagactaagt gcgtgatcat
     3961 gtaaaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
     4021 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     4081 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4141 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4201 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4261 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4321 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4561 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4681 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4801 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4861 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4921 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5041 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5101 ttggaggcct aggcttttgc aaaaagctcc catgaccgag tacaagccca cggtgcgcct
     5161 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     5221 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     5281 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5341 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5401 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5461 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5521 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5581 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5641 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5701 ctggtgcatg acccgcaagc ccggtgcctg attcgaaatg accgaccaag cgacgcccaa
     5761 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5821 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5881 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5941 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6001 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     6061 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     7021 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7081 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7141 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7201 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7261 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7321 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7381 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7441 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7501 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7561 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7621 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7681 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7741 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7801 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7861 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7921 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7981 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8041 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//