LOCUS       pSELIS-RamR             5966 bp ds-DNA     circular     17-MAY-2023
DEFINITION  .
ACCESSION   urn.local...1363478693202.151
VERSION     urn.local...1363478693202.151
COMMENT     Imported using the Genbank importer. File name: pPOS5 (1).gb
FEATURES             Location/Qualifiers
     misc_feature    complement(36..875)
                     /label="p15A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1234..1893
                     /label="CAT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     terminator      complement(2114..2161)
                     /label="LuxI terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2165..2539)
                     /label="EZeo"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(2165..2539)
                     /label="Translation 2539-2165"
                     /translation="MSKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD*"
     misc_feature    complement(2545..2565)
                     /label="RBS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Promoter        complement(2593..2641)
                     /label="Lambda promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2599..2604)
                     /label=-10
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2622..2627)
                     /label=-35
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      2667..2700
                     /label="BBa_B1001 Bidirectional Term"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2720..2778
                     /label="RamR Promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2732..2737
                     /label=-35
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2754..2759
                     /label=-10
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2757..2778
                     /label="RamR operator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2780..2799
                     /label="RBS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2806..3516
                     /label="cI Repressor"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2806..3516
                     /label="Translation 2806-3516"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVGKVIASQWPEETF*"
     terminator      3543..3605
                     /label="L3S2P00 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3634..3694
                     /label="RamR-promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3648..3653
                     /label=-35
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3670..3675
                     /label=-10
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3673..3694
                     /label="RamR operator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3695..3716
                     /label="RBS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3723..4466
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      4470..4598
                     /label="rrnb.TE Tandem Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4713..5407
                     /label="LacI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tttgaagaga taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct
       61 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
      121 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
      181 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
      241 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
      301 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
      361 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
      421 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      481 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
      541 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
      601 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
      661 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
      721 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
      781 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
      841 ccaacatagt aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt
      901 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
      961 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca
     1021 ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cannataaat aaatcctggt
     1081 gtccctgttg ataccgggaa gccctggcca actttggcga aaatgagacg tgatcggcac
     1141 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1201 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1261 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1321 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1381 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1441 catccggagt tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1501 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1561 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1621 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1681 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1741 gttttcacta tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1801 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1861 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1921 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1981 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
     2041 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
     2101 cctgaggttt cagaaataat aaaaaagccg gattaataat ctggcttttt atattctctc
     2161 tttaTTAATC TTGTTCTTCA GCAACAAAAT GAACGCAATT ACCAGCTGGA TCTCTCAAAG
     2221 CAAATTCTCT ACCCCAAGGT TGTTCACCAA TTTCGGTCAT AGCTGGACCA GAAGCATCTC
     2281 TAAAATTGGT AGAAACAACT TCGGACCATT CGGCATATAA TTCATCCAAA CCTCTAACCC
     2341 AAACCCAAGC CAAAGTATTA TCTGGAACAA CTTGATCTTG AACGGCGGAA ATGAACAAGG
     2401 TAACATCATC TCTAACAACA CCAGCGAAAT CATCTTCAAC GAAATCTCTG GAGAAACCCA
     2461 ATCTATCAGT CCAAAATTCA ACAGCACCAG CAACATCTCT AGCGGTCAAA ACTGGAACAG
     2521 CAGATGTCAA TTTAGACATa tgtcAACCTC CTCCAATAAC CGTCActgct gacgactctA
     2581 CCAttatcaC ttgcaaccat tatcaccgcc agaggtaaaa tagtcaacac gcacggtgtt
     2641 agtcttttcg ccggtgtatt tcttgcaaaa aaaaaccccg ccgaagcggg gttttttttt
     2701 ttacagggct ataaggtaga tccgggagct gTTGACGGCG TATCTTTGCT TTCTATAATG
     2761 AGTGCTTACT CACTCATAAT CAAGtactag agaaggagat ctagaatgag cacaaaaaag
     2821 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa
     2881 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag
     2941 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg
     3001 cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc
     3061 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct
     3121 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt
     3181 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag
     3241 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg
     3301 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt
     3361 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa
     3421 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa
     3481 gttatcgcta gtcagtggcc tgaagagacg tttTAAcgat aatgtcgggc aatcaggtgc
     3541 gactcggtac caaattccag aaaagagggg agcgggaaac cgctcccctt ttttcgtttt
     3601 ggtcccaatc tatcgattgt atgggctgtc gacCTTTGAA AAGTACCTTG ACGGCGTATC
     3661 TTTGCTTTCT ATAATGAGTG CTTACTCACT CATAGGGTTT GATTTAATAA GGAGGTatac
     3721 atatggcatc caagggcgag gagctcttta ctggcgtagt accaattctc gtagagctcg
     3781 atggcgatgt aaatggccat aagttttccg tacgcggcga gggcgagggc gatgcaacta
     3841 acggcaagct cactctcaag tttatttgta ctactggcaa gctcccagta ccatggccaa
     3901 ctctcgtaac tactctgacc tatggcgtac aatgtttttc ccgctatcca gatcacatga
     3961 agcaacatga tttttttaag tccgcaatgc cagagggcta tgtacaagag cgcactatta
     4021 gctttaagga tgatggcacc tataagactc gcgcagaggt aaagtttgag ggcgatactc
     4081 tcgtaaatcg cattgagctc aagggcattg attttaagga ggatggcaat attctcggcc
     4141 ataagctgga gtataatttc aattcccata atgtatatat taccgcagat aagcaaaaga
     4201 atggcattaa ggcgaatttt aagattcgcc ataatgtgga ggatggctcc gtacaactcg
     4261 cagatcatta tcaacaaaat actccaattg gcgatggccc agtactcctc ccagataatc
     4321 attatctctc cactcaatcc gtgctctcca aagatccaaa tgagaagcgc gatcacatgg
     4381 tactcctgga gtttgtaact gcagcaggca ttactcatgg catggatgag ctctataagc
     4441 tcgagcacca ccaccaccac cactAATAAc caggcatcaa ataaaacgaa aggctcagtc
     4501 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     4561 cactggctca ccttcgggtg ggcctttctg cgtttataat ttcctaatgc aggagtcgca
     4621 taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
     4681 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
     4741 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     4801 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     4861 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     4921 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     4981 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     5041 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     5101 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     5161 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     5221 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     5281 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     5341 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     5401 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     5461 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     5521 acatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
     5581 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     5641 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     5701 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5761 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5821 gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac
     5881 ccagtcagct ccttccggtg ggcgcggggc atgactaaca tgagaattac aacttatatc
     5941 gtatggggct gacttcaggt gctaca
//