LOCUS       pSB-CMV-MCS-Puro        7696 bp    DNA     circular     18-MAY-2023
SOURCE      
  ORGANISM  
COMMENT     izraeli csoporttol, eredeti nev: pT2-HB-CAG-AMAXA
            http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14802466|
COMMENT     VNTDBDATE|-14923433|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSB-CMV-MCS-Puro GEM-LTB4|
COMMENT     VNTAUTHORNAME|Borbély Csilla|
COMMENT     VNTOAUTHORNAME|Eszter&Tamas|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSB-CMV-MCS-Puro GEM-LTB4" 0 7696 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "Acc65I" "AflII" "AgeI" "ApaI" "ApaLI" "AseI" "AvaII" 
COMMENT         "BamHI" "BbsI" "BclI" "BglII" "BsaI" "BsmBI" "BspEI" "BsrGI" "BstBI" 
COMMENT         "BstXI" "Bsu36I" "ClaI" "DpnI" "EcoRI" "EcoRV" "HindIII" "KpnI" "MfeI" 
COMMENT         "MluI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "PacI" "PmeI" "PspOMI" "PstI" 
COMMENT         "PvuI" "PvuII" "SacI" "SalI" "SmaI" "SpeI" "XbaI" "XhoI") 
COMMENT        (CStringList "atg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            4538 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AflII" "cttaag" 2 0 4 684 0 2170 0 4173 0 7440 
COMMENT            0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 2714 0 4536 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 5250 0 6496 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 7 1716 0 2964 0 3474 0 3584 
COMMENT            0 3916 0 5967 0 6189 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 705 0 746 0 4501 0 7403 
COMMENT            0 0 "") #6=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 2728 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SpeI" "actagt" 2 0 3 25 0 711 0 752 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 652 1 3832 1 5890 1 0 
COMMENT             "") #11=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1769 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 1684 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 4 724 0 1254 0 2517 
COMMENT             0 2687 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 1 3954 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 4184 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 728 0 2665 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2677 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 2 0 687 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 4 697 0 1374 0 1784 0 4542 
COMMENT             0 0 "") 
COMMENT        #21=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 4090 0 7562 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 2459 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 386 0 764 0 3221 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 260 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 671 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2692 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 738 0 1484 0 2674 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 2990 0 4760 0 7273 0 0 
COMMENT             "") 
COMMENT        #29=(CRSite (CStringList) "SacI" "gagctc" 6 0 5 594 0 703 0 2657 0 3969 
COMMENT             0 7401 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 2252 0 3466 0 4517 0 0 
COMMENT             "") 
COMMENT        #31=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 742 0 3409 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1194 0 2704 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 3 2698 0 3962 0 4523 0 0 
COMMENT             "") #34=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 856 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 6199 0 7244 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "AseI" "attaat" 3 0 5 33 0 638 0 4707 0 4766 0 
COMMENT             6001 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 2930 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "DpnI" "gatc" 3 0 27 707 0 748 0 1709 0 1747 0 
COMMENT             2237 0 2730 0 3628 0 3960 0 4183 0 4193 0 4383 0 4503 0 5504 0 5579 
COMMENT             0 5590 0 5598 0 5676 0 5688 0 5793 0 6134 0 6152 0 6198 0 6456 0 
COMMENT             6473 0 6509 0 7243 0 7405 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 2 680 0 2719 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 2 2710 0 4532 0 0 "")) 
COMMENT       (CObList 
COMMENT        #43=(CFSignal (CObList) "ori" 33 0 0 4814 5694 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4814..5694") 
COMMENT        #44=(CFSignal (CObList) "Amp" 4 0 1 5758 6615 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5758..6615)") 
COMMENT        #45=(CFSignal (CObList) "F1 ori" 33 0 0 6746 7202 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6746..7202") 
COMMENT        #46=(CFSignal (CObList) "T3" 30 0 1 4554 4573 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4554..4573)") 
COMMENT        #47=(CFSignal (CObList) "T7" 30 0 0 7368 7388 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7368..7388") 
COMMENT        #48=(CFSignal (CObList) "IR-DR(L)" 44 0 1 7413 7639 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7413..7639)") 
COMMENT        #49=(CFSignal (CObList) "IR-DR(R)" 44 0 0 4267 4494 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4267..4494") 
COMMENT        #50=(CFSignal (CObList) "polyA" 25 0 0 4069 4074 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4069..4074") 
COMMENT        #51=(CFSignal (CObList) "BGH pA" 26 0 0 2740 2964 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1870000\"") 1 1 1 1 "2740..2964") 
COMMENT        #52=(CFSignal (CObList) "BGH reverse primer" 27 0 1 2734 2751 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1910000\"") 1 1 1 1 
COMMENT             "complement(2734..2751)") 
COMMENT        #53=(CFSignal (CObList) "CMV forward primer" 27 0 0 544 564 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1930000\"") 1 1 1 1 
COMMENT             "544..564") 
COMMENT        #54=(CFSignal (CObList) "CMV promoter" 29 0 0 7 594 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2180000\"") 1 1 1 1 "7..594") 
COMMENT        #55=(CFSignal (CObList) "T7 primer" 27 0 0 638 657 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2100000\"") 1 1 1 1 "638..657") 
COMMENT        #56=(CFSignal (CObList) "T7 promoter" 30 0 0 638 654 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 "638..654") 
COMMENT        #57=(CFSignal (CObList) "pBABE_3_primer" 21 0 1 2994 3014 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2994..3014)") 
COMMENT        #58=(CFSignal (CObList) "SV40_enhancer" 21 0 1 3000 3215 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3000..3215)") 
COMMENT        #59=(CFSignal (CObList) "SV40_promoter" 30 0 0 3012 3280 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3012..3280") 
COMMENT        #60=(CFSignal (CObList) "SV40_origin" 33 0 0 3179 3256 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3179..3256") 
COMMENT        #61=(CFSignal (CObList) "SV40_promoter" 30 0 0 3132 3334 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3132..3334") 
COMMENT        #62=(CFSignal (CObList) "SV40pro_F_primer" 21 0 0 3241 3260 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3241..3260") 
COMMENT        #63=(CFSignal (CObList) "puro(variant)" 4 0 0 3357 3956 0 (CStringList) 
COMMENT             (CStringList "/gene=\"puro(variant)\"") 1 1 1 1 "3357..3956") 
COMMENT        #64=(CFSignal (CObList) "GEM-LTB4" 21 0 0 765 2663 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pSB-CMV-MCS-Puro GEM-LTB4'" 1) 
COMMENT                     #69=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: Borb\351ly Csilla. Original author: Eszter&Tamas" 
COMMENT                          1) 
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COMMENT                          "Created: 02/23/23 10:01de." 1) 
COMMENT                     #71=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/18/23 11:08de." 1) 
COMMENT                     #72=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7696 bp" 1) 
COMMENT                     #73=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "Modification Date in the Original DB: 18-MAY-2023" 1) 
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COMMENT                          "Associated Genes:  puro(variant)" 1))) 
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COMMENT                          5 "CTTAAG" "GAATTC") 
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COMMENT                                        " N1: 856 " 1))))) "AgeI: 1 site") 1 5 
COMMENT                          "ACCGGT" "TGGCCA") 
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COMMENT                          "GGGCCC" "CCCGGG") 
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COMMENT                           12 "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                           "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                           "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                           "AGGCCT") 
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COMMENT                           1 5 "TGTACA" "ACATGT") 
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COMMENT                           2 4 "TTCGAA" "AAGCTT") 
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COMMENT                                     #151=(CLinePar 
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COMMENT                                           " N4: 2687 " 1))))) "BstXI: 4 sites") 
COMMENT                           8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                           2 5 "CCTNAGG" "GGANTCC") 
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COMMENT                                           " N1: 4184 " 1))))) "ClaI: 1 site") 2 
COMMENT                           4 "ATCGAT" "TAGCTA") 
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COMMENT                                           " N1: 707 " 1) 
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COMMENT                                           " N3: 1709 " 1) 
COMMENT                                     #163=(CLinePar 
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COMMENT                                           " N4: 1747 " 1) 
COMMENT                                     #164=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 2237 " 1) 
COMMENT                                     #165=(CLinePar 
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COMMENT                                           " N6: 2730 " 1) 
COMMENT                                     #166=(CLinePar 
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COMMENT                                     #167=(CLinePar 
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COMMENT                                           " N8: 3960 " 1) 
COMMENT                                     #168=(CLinePar 
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COMMENT                                           " N9: 4183 " 1) 
COMMENT                                     #169=(CLinePar 
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COMMENT                                           " N10: 4193 " 1) 
COMMENT                                     #170=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 11) 1 2 2 0 191) 
COMMENT                                           " N11: 4383 " 1) 
COMMENT                                     #171=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 12) 1 2 2 0 191) 
COMMENT                                           " N12: 4503 " 1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 13) 1 2 2 0 191) 
COMMENT                                           " N13: 5504 " 1) 
COMMENT                                     #173=(CLinePar 
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COMMENT                                           " N14: 5579 " 1) 
COMMENT                                     #174=(CLinePar 
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COMMENT                                           " N15: 5590 " 1) 
COMMENT                                     #175=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 16) 1 2 2 0 191) 
COMMENT                                           " N16: 5598 " 1) 
COMMENT                                     #176=(CLinePar 
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COMMENT                                           " N17: 5676 " 1) 
COMMENT                                     #177=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 18) 1 2 2 0 191) 
COMMENT                                           " N18: 5688 " 1) 
COMMENT                                     #178=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 19) 1 2 2 0 191) 
COMMENT                                           " N19: 5793 " 1) 
COMMENT                                     #179=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 20) 1 2 2 0 191) 
COMMENT                                           " N20: 6134 " 1) 
COMMENT                                     #180=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 21) 1 2 2 0 191) 
COMMENT                                           " N21: 6152 " 1) 
COMMENT                                     #181=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 22) 1 2 2 0 191) 
COMMENT                                           " N22: 6198 " 1) 
COMMENT                                     #182=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 23) 1 2 2 0 191) 
COMMENT                                           " N23: 6456 " 1) 
COMMENT                                     #183=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 24) 1 2 2 0 191) 
COMMENT                                           " N24: 6473 " 1) 
COMMENT                                     #184=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 25) 1 2 2 0 191) 
COMMENT                                           " N25: 6509 " 1) 
COMMENT                                     #185=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 26) 1 2 2 0 191) 
COMMENT                                           " N26: 7243 " 1) 
COMMENT                                     #186=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #39# 27) 1 2 2 0 191) 
COMMENT                                           " N27: 7405 " 1))))) "DpnI: 27 sites") 
COMMENT                           2 2 "GATC" "CTAG") 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 728 " 1) 
COMMENT                                     #190=(CLinePar 
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COMMENT                                           " N2: 2665 " 1))))) "EcoRI: 2 sites") 
COMMENT                           1 5 "GAATTC" "CTTAAG") 
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COMMENT                           3 3 "GATATC" "CTATAG") 
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COMMENT                                           " N1: 2 " 1) 
COMMENT                                     #197=(CLinePar 
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COMMENT                                           " N2: 687 " 1))))) "HindIII: 2 sites") 
COMMENT                           1 5 "AAGCTT" "TTCGAA") 
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COMMENT                             (CObjectList 
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COMMENT                                           " N1: 697 " 1) 
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COMMENT                                           " N2: 1374 " 1) 
COMMENT                                     #202=(CLinePar 
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COMMENT                                           " N3: 1784 " 1) 
COMMENT                                     #203=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #20# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 4542 " 1))))) "KpnI: 4 sites") 
COMMENT                           5 1 "GGTACC" "CCATGG") 
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COMMENT                            (CGroupPar (CParagraph 118771632 (8 0) 1 1 1 0 178) 
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COMMENT                                           " N1: 4090 " 1) 
COMMENT                                     #207=(CLinePar 
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COMMENT                                           " N2: 7562 " 1))))) "MfeI: 2 sites") 
COMMENT                           1 5 "CAATTG" "GTTAAC") 
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COMMENT                                    (CObjectList 
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COMMENT                           5 "ACGCGT" "TGCGCA") 
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COMMENT                                           " N1: 386 " 1) 
COMMENT                                     #214=(CLinePar 
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COMMENT                                           " N2: 764 " 1) 
COMMENT                                     #215=(CLinePar 
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COMMENT                                           " N3: 3221 " 1))))) "NcoI: 3 sites") 
COMMENT                           1 5 "CCATGG" "GGTACC") 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 260 " 1))))) "NdeI: 1 site") 2 
COMMENT                           4 "CATATG" "GTATAC") 
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COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 671 " 1))))) "NheI: 1 site") 1 
COMMENT                           5 "GCTAGC" "CGATCG") 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 2692 " 1))))) "NotI: 1 site") 2 
COMMENT                           6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118770416 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "NruI: 0 sites") 3 3 "TCGCGA" 
COMMENT                           "AGCGCT") 
COMMENT                     #226=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118773040 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                           "AATTAATT") 
COMMENT                     #227=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118773104 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #229=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 680 " 1) 
COMMENT                                     #230=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #41# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2719 " 1))))) "PmeI: 2 sites") 
COMMENT                           4 4 "GTTTAAAC" "CAAATTTG") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118773520 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 2710 " 1) 
COMMENT                                     #234=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #42# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 4532 " 1))))) "PspOMI: 2 sites") 
COMMENT                           1 5 "GGGCCC" "CCCGGG") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772688 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 738 " 1) 
COMMENT                                     #238=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #27# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 1484 " 1) 
COMMENT                                     #239=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #27# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2674 " 1))))) "PstI: 3 sites") 
COMMENT                           5 1 "CTGCAG" "GACGTC") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118773296 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #242=(CLinePar 
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COMMENT                                           " N1: 6199 " 1) 
COMMENT                                     #243=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #35# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 7244 " 1))))) "PvuI: 2 sites") 
COMMENT                           4 2 "CGATCG" "GCTAGC") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772720 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #245=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #28# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #246=(CLinePar 
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COMMENT                                           " N1: 2990 " 1) 
COMMENT                                     #247=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #28# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 4760 " 1) 
COMMENT                                     #248=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #28# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 7273 " 1))))) "PvuII: 3 sites") 
COMMENT                           3 3 "CAGCTG" "GTCGAC") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772592 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #250=(CGroupPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #251=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #29# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 594 " 1) 
COMMENT                                     #252=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #29# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 703 " 1) 
COMMENT                                     #253=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #29# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2657 " 1) 
COMMENT                                     #254=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #29# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 3969 " 1) 
COMMENT                                     #255=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #29# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 7401 " 1))))) "SacI: 5 sites") 
COMMENT                           5 1 "GAGCTC" "CTCGAG") 
COMMENT                     #256=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772496 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #257=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #30# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #258=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #30# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2252 " 1) 
COMMENT                                     #259=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #30# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3466 " 1) 
COMMENT                                     #260=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #30# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 4517 " 1))))) "SalI: 3 sites") 
COMMENT                           1 5 "GTCGAC" "CAGCTG") 
COMMENT                     #261=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772976 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #262=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #31# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #263=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #31# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 742 " 1) 
COMMENT                                     #264=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #31# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3409 " 1))))) "SmaI: 2 sites") 
COMMENT                           3 3 "CCCGGG" "GGGCCC") 
COMMENT                     #265=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118770448 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #266=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #267=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 25 " 1) 
COMMENT                                     #268=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 711 " 1) 
COMMENT                                     #269=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 752 " 1))))) "SpeI: 3 sites") 1 
COMMENT                           5 "ACTAGT" "TGATCA") 
COMMENT                     #270=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772560 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #271=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #32# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #272=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #32# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1194 " 1) 
COMMENT                                     #273=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #32# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2704 " 1))))) "XbaI: 2 sites") 
COMMENT                           1 5 "TCTAGA" "AGATCT") 
COMMENT                     #274=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 118772880 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #275=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #33# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #276=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #33# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2698 " 1) 
COMMENT                                     #277=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #33# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3962 " 1) 
COMMENT                                     #278=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #33# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 4523 " 1))))) "XhoI: 3 sites") 
COMMENT                           1 5 "CTCGAG" "GAGCTC"))) 
COMMENT                   "Restriction/Methylation Map") 
COMMENT              #279=(CIvgnResourcePar 
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FEATURES             Location/Qualifiers
     rep_origin      4814..5694
                     /vntifkey="33"
                     /label=ori
     CDS             complement(5758..6615)
                     /vntifkey="4"
                     /label=Amp
     rep_origin      6746..7202
                     /vntifkey="33"
                     /label=F1\ori
     promoter        complement(4554..4573)
                     /vntifkey="30"
                     /label=T3
     promoter        7368..7388
                     /vntifkey="30"
                     /label=T7
     transposon      complement(7413..7639)
                     /vntifkey="44"
                     /label=IR-DR(L)
     transposon      4267..4494
                     /vntifkey="44"
                     /label=IR-DR(R)
     polyA_signal    4069..4074
                     /vntifkey="25"
                     /label=polyA
     polyA_site      2740..2964
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(2734..2751)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     primer          544..564
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        7..594
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     primer          638..657
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        638..654
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    complement(2994..3014)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3000..3215)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3012..3280
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3179..3256
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        3132..3334
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    3241..3260
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3357..3956
                     /gene="puro(variant)"
                     /vntifkey="4"
                     /label=puro(variant)
     misc_feature    765..2663
                     /vntifkey="21"
                     /label=GEM-LTB4
BASE COUNT     1754 a      2118 c      2020 g      1804 t 
ORIGIN
        1 aagcttgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 
      661 cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt 
      721 gtggtggaat tcctgcagcc cgggggatcc actagttcgc caccatggag acagacacac 
      781 tcctgctatg ggtactgctg ctctgggttc caggttccac tggtgacacc agcctgtata 
      841 agaaggtggg aaccaccggt aacactacat cttctgccgc acccccctca ctaggtgtag 
      901 agttcatctc tctgctggct atcatcctgc tgtcagtggc gctggctgtg gggcttcccg 
      961 gcaacagctt tgtggtgtgg agtatcctga aaaggatgca gaagcgctct gtcactgccc 
     1021 tgatggtgct gaacctggcc ctggccgacc tggccgtatt gctcactgct ccctttttcc 
     1081 ttcacttcct ggcccaaggc acctggagtt ttggactggc tggttgccgc ctgtgtcact 
     1141 atgtctgcgg agtcagcatg tacgccagcg tcctgcttat cacggccatg agtctagacc 
     1201 gctcactggc ggtggcccgc ccctttgtgt cccagaagct acgcaccaag gcgatggccc 
     1261 ggcgggtgct ggcaggcatc tgggtgttgt cctttctgct ggccacaccc gtcctcgcgt 
     1321 accgcacagt agtgccctgg aaaacgaaca tgagcctgtg cttcccgcgg taccccagcg 
     1381 aagggcaccg ggccttccat ctaatcttcg aggctgtcac gggcttcctg ctgcccttcc 
     1441 tggctgtggt ggccagctac tcggacatag ggcgtcggct gcaggcccgg cgcagcagcc 
     1501 tgattaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca 
     1561 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc 
     1621 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac 
     1681 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 
     1741 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga 
     1801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 
     1861 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc 
     1921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 
     1981 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 
     2041 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg 
     2101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 
     2161 gcatcgagct taagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 
     2221 agtacaacaa ccacgatcag ttccgccgca gtcgacgcac cggccgcctg gtggtgctca 
     2281 tcatcctgac cttcgccgcc ttctggctgc cctaccacgt ggtgaacctg gctgaggcgg 
     2341 gccgcgcgct ggccggccag gccgccgggt tagggctcgt ggggaagcgg ctgagcctgg 
     2401 cccgcaacgt gctcatcgca ctcgccttcc tgagcagcag cgtgaacccc gtgctgtacg 
     2461 cgtgcgccgg cggcggcctg ctgcgctcgg cgggcgtggg cttcgtcgcc aagctgctgg 
     2521 agggcacggg ctccgaggcg tccagcacgc gccgcggggg cagcctgggc cagaccgcta 
     2581 ggagcggccc cgccgctctg gagcccggcc cttccgagag cctcactgcc tccagccctc 
     2641 tcaagttaaa cgagctcaac taagaattct gcagatatcc agcacagtgg cggccgctcg 
     2701 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 
     2761 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 
     2821 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 
     2881 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 
     2941 ctggggatgc ggtgggctct atggaccgga ctgaggcgga aagaaccagc tgtggaatgt 
     3001 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     3061 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 
     3121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     3181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     3241 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 
     3301 cttttttgga ggcctaggct tttgcaaaaa gctagatttt tctgacccta gccaccatga 
     3361 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca 
     3421 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc 
     3481 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 
     3541 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 
     3601 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 
     3661 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 
     3721 cgtggttcct ggccaccgtc ggcgtgtcgc ccgaccacca gggcaagggt ctgggcagcg 
     3781 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 
     3841 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 
     3901 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggat 
     3961 ctcgagctcg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 
     4021 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 
     4081 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 
     4141 taaagcaagt aaaacctcta caaatgtggt acttaagagg gatcgataag gatctagctt 
     4201 gtggaaggct actcgaaatg tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa 
     4261 tactaattga gtgtatgtaa acttctgacc cactgggaat gtgatgaaag aaataaaagc 
     4321 tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa ataaagtggt 
     4381 gatcctaact gacctaagac agggaatttt tactaggatt aaatgtcagg aattgtgaaa 
     4441 aagtgagttt aaatgtattt ggctaaggtg tatgtaaact tccgacttca actgtatagg 
     4501 gatcctctag ctagagtcga cctcgagggg gggcccggta cccagctttt gttcccttta 
     4561 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     4741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     4801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     4861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     4921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     4981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     5041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     5101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     5161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     5221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     5281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     5341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     5401 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     5461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     5521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     5581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     5641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     5701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     5761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     5821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     5881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     5941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     6001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     6061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     6121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     6181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     6241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     6301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     6361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     6421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     6481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     6541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     6601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     6661 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     6721 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 
     6781 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     6841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     6901 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     6961 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     7021 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     7081 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     7141 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 
     7201 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     7261 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 
     7321 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca 
     7381 ctatagggcg aattggagct cggatcccta tacagttgaa gtcggaagtt tacatacact 
     7441 taagttggag tcattaaaac tcgtttttca actactccac aaatttcttg ttaacaaaca 
     7501 atagttttgg caagtcagtt aggacatcta ctttgtgcat gacacaagtc atttttccaa 
     7561 caattgttta cagacagatt atttcactta taattcactg tatcacaatt ccagtgggtc 
     7621 agaagtttac atacactaag ttgactgtgc ctttaaacag cttggaaaat tccagaaaat 
     7681 gatgtcatgg ctttag 
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