LOCUS       WR9                     8155 bp ds-DNA     circular     18-MAY-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(7162..8019)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5174..6321)
                     /locus_tag="Ps ARS from Chsome1"
                     /label="Ps ARS from Chsome1"
                     /ApEinfo_label="Ps ARS from Chsome1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3053..4681
                     /locus_tag="PsCO CBG99"
                     /label="PsCO CBG99"
                     /ApEinfo_label="PsCO CBG99"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6344..7011
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1836..2211
                     /locus_tag="Act term"
                     /label="Act term"
                     /ApEinfo_label="Act term"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             777..1805
                     /locus_tag="PsCOhph"
                     /label="PsCOhph"
                     /ApEinfo_label="PsCOhph"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        45..770
                     /locus_tag="Ps TEF1 promoter"
                     /label="Ps TEF1 promoter"
                     /ApEinfo_label="Ps TEF1 promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2292..3046
                     /locus_tag="Ptdh3"
                     /label="Ptdh3"
                     /ApEinfo_label="Ptdh3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4687..5061
                     /locus_tag="Adh2term"
                     /label="Adh2term"
                     /ApEinfo_label="Adh2term"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CCAATGATAC CGGTGCGATC GTGGGCCCTA GTACTTCCCT CGAGCCGTAC ACTTATTGTT
       61 AACTATGAAA CATATACTTA TGGACAAGGT TCACTGATTT GGACTGAAAA GCAAAAACGA
      121 GAAATGAGTC AACGTCTGAG ATCAATCCCT AGACCACCCG GGAAGGTCAT GTGTTTCATG
      181 AAGTACGATA AGGTTGGTAA CCGATTGACT CATTGGTTCG TGGCGGAGAA GTACGCAGAG
      241 TAAAACCGGG GCCGATTCGT GGTAAATTCT GGAATGATCC AGAGGCGCGA CATTTATGCA
      301 GACAATTTGT GTTTTGTCGC AAACGATGTT ATAGCGAAAT TTTTCACTCT GTCAGATAAA
      361 TGGATTTTGT CAAAAGGGGG AAGTAGAAGG AGAATGGGCC CGAGATGTTC TGCCAAATTC
      421 TCAGTAGCAT AATGTGAAAG AAGCCCTTAC ATTGTCCAGC CTCTGGCATC ATTAAAAACC
      481 GTAGCGGAAA CCAATTGTCT CTGTTCTTCC CTGGCACACC CTGGTAGCCC CATCCAGTTG
      541 TAGTACATCT CACACGCTGG CAACTTGGGA CAATCAGCAA CTTTTTTTTC TTTTAATTTT
      601 TTCAGCGCGA CATTTTGCCT CTTCTGCGAG AACAGACTTT TTCACCTCCA TCTCACCCCC
      661 CTTTGCACTT ATATAAATTG GACCAGTTCC TCCCATTGTA GAAAAAATTT TGCTGGACCT
      721 TTTTCTCTTT TTTTTGTCCT TTAGTTTCAT ACAATCTAAG TCTATCTACA TGTACAATGG
      781 GTAAAAAGCC TGAACTCACC GCGACcTCTG TCGAGAAGTT TTTGATCGAA AAGTTCGACA
      841 GCGTCTCCGA CTTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG
      901 TAGGAGGGCG TGGATATGTC TTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC
      961 GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG
     1021 GGGAGTTCAG CGAGAGCTTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC
     1081 AAGACTTGCC TGAAACCGAA TTGCCCGCTG TTTTGCAGCC GGTCGCGGAG GCCATGGATG
     1141 CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA
     1201 TCGGTCAATA CACTACATGG CGTGATTTCA TTTGCGCGAT TGCTGATCCC CATGTGTATC
     1261 ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGT
     1321 TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT
     1381 CCAACAATGT CTTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA
     1441 TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
     1501 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCAC
     1561 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
     1621 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
     1681 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
     1741 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
     1801 AATAAGCGGC CGCtAACCAC TTGCAAAATC CTTTGTATTC TTGTTCTGCA AACATTTTGT
     1861 CCAATCTCTT ATCTTTCTAC GATGTTGAAG AACATACATT TTTGTTAGTC CAGCTTGTTA
     1921 TCCTTTTTTT ATTAAATAAA AAATTTTATT GTTAAAGTTG TTTTTCTTCT TTGTCTGTCC
     1981 TGTTTTAACT CATATATATT CTATATATTA CAGAAAGAAA GTAGATCATC TAAAATAAAC
     2041 ATTTATTTGA TGAGACAAAG TAGGTTTTTT AGAATACAAT GTAGCATCCT CTAATATACA
     2101 ATAGAGTTGT TTTATAAAAG CGATACATTT AATATTATGA CAGGACTGGT GAAGTGTATA
     2161 GAATTGTGCT AATCTGGCAA ACGAACCCAA GAAGACGTTA AAGCAAATAG TGCATTTTGT
     2221 ATTACAATCA AGTTCGTCGA CCATGCATTA GATCTTGCAT GCCTCTAGAA CCTAGGTGGC
     2281 GCCACAGCTG TGGATCCTCG TTAGTATTTC CGTGAAGGCA GCCATTGTGA AAATTGAACA
     2341 TCACCTGAGG TATTTTAGCC ACCAGAAGCG GCGGTACGGA AGAAAGTGTG TACAATGGTT
     2401 GGTGGTGGAA TTGCGTGCAT GCCTGATGGG GCAATATTAA TTAGATAGAG CTTTGGTGAT
     2461 ATTAGTGGAT AATAGAATTC ACAGAGAAGA CATCAGGAGC AATTTCCAAG AGCCATTGAT
     2521 GATGTAATTG CCCCAACAGC AAGATTCAGA TCTGACAATT GACCACCGTT TTGTAGAAGC
     2581 AAAAAATCGT AGATTATCAC CAAGAGGGTT TTTCACCGAA CCAGCAAATA GAAACTATTC
     2641 CGTAGAACTC GCCCAGGCTT TTTTGCTAGC ACTTTCCAGC AGTAGAACCG TCCAATTAAG
     2701 TCAACAGGAA CCATTGAGGT CGAGCCCAAC CACCTGAACC CCCTCACGGT CGTGTCCCTA
     2761 TTATTGATCC AGAGGGTGCC AGTTTCGGTA GCCAATATTG GTTCATGGGT TTCTATGGCC
     2821 CGGAGTGAGT TTGCAGGTTG GCCCCGGCGC CGTCTGCAGG ATGGGAGTTA TAGCGGCCAA
     2881 ACTTCACATT TCGAAATCCT GCTGCAGCCA ATCTGAAGAA TTAATATAAA TTCGTGTCGA
     2941 ATCGCCGTCT GTGAAATTTC AGTACTTGAT TTTCTTTTCT TCTTCTTTTT CTCTTTTGTT
     3001 TCTTCAGAAT CAATTCACAT TTTTTCTTCC CTATAAACAA TTCATCGGTA CCATGGTGAA
     3061 GCGTGAGAAA AATGTCATCT ATGGCCCTGA GCCTCTCCAT CCTTTGGAGG ATTTGACTGC
     3121 CGGCGAAATG TTGTTTCGTG CTCTCCGCAA GCACTCTCAT TTGCCTCAAG CCTTGGTCGA
     3181 TGTGGTCGGC GATGAATCTT TGAGCTACAA GGAGTTTTTT GAGGCAACCG TCTTGTTGGC
     3241 TCAGTCCCTC CACAATTGTG GCTACAAGAT GAACGACGTC GTTAGTATCT GTGCTGAAAA
     3301 CAATACCCGT TTCTTCATTC CAGTCATCGC CGCATGGTAT ATCGGTATGA TCGTGGCTCC
     3361 AGTCAACGAG AGCTACATTC CCGACGAATT GTGTAAAGTC ATGGGTATCT CTAAGCCACA
     3421 GATTGTCTTC ACCACTAAGA ATATTTTGAA CAAAGTCTTG GAAGTCCAAA GCCGCACCAA
     3481 CTTTATTAAG CGTATCATCA TCTTGGACAC TGTGGAGAAT ATTCACGGTT GCGAATCTTT
     3541 GCCTAATTTC ATCTCTCGCT ATTCAGACGG CAACATCGCA AACTTTAAAC CACTCCACTT
     3601 CGACCCTGTG GAACAAGTTG CAGCCATTTT GTGTAGCAGC GGTACTACTG GACTCCCAAA
     3661 GGGAGTCATG CAGACCCATC AAAACATTTG CGTGCGTTTG ATCCATGCTC TCGATCCACG
     3721 tGTGGGCACT CAGTTGATTC CTGGTGTCAC CGTCTTGGTC TACTTGCCTT TCTTCCATGC
     3781 TTTCGGCTTT AGCATTACTT TGGGTTACTT TATGGTCGGT CTCCGCGTGA TTATGTTCCG
     3841 CCGTTTTGAT CAGGAGGCTT TCTTGAAAGC CATCCAAGAT TATGAAGTCC GCAGTGTCAT
     3901 CAACGTGCCT AGCGTGATCT TGTTTTTGTC TAAGAGCCCA CTCGTGGACA AGTACGACTT
     3961 GTCTTCAtTG CGTGAATTGT GTTGCGGTGC CGCTCCAtTG GCTAAGGAGG TCGCTGAAGT
     4021 GGCCGCCAAA CGCTTGAATC TTCCAGGtAT TCGTTGTGGC TTCGGCCTCA CCGAATCTAC
     4081 CAGCGCTAAC ATTCACTCTC TCGGtGATGA GTTTAAGAGC GGCTCTTTGG GCCGTGTCAC
     4141 TCCACTCATG GCTGCTAAGA TCGCTGATCG CGAAACTGGT AAGGCTTTGG GtCCGAACCA
     4201 AGTGGGCGAG tTGTGTATCA AAGGCCCTAT GGTGAGCAAG GGTTATGTCA ATAACGTTGA
     4261 AGCTACCAAG GAGGCCATCG ACGACGACGG CTGGTTGCAT TCTGGTGATT TTGGATATTA
     4321 CGACGAAGAT GAGCATTTTT ACGTCGTGGA TCGTTACAAG GAGTTGATCA AATACAAGGG
     4381 TAGCCAGGTT GCTCCAGCTG AGTTGGAGGA GATTTTGTTG AAAAATCCAT GCATTCGCGA
     4441 TGTCGCTGTG GTCGGCATTC CTGATTTGGA GGCCGGCGAA TTGCCTTCTG CTTTCGTTGT
     4501 CAAGCAGCCT GGTAAAGAAA TTACCGCCAA AGAAGTGTAT GATTACTTGG CTGAACGTGT
     4561 GAGCCATACT AAGTACTTGC GTGGCGGCGT GCGTTTTGTT GACTCCATCC CTCGTAACGT
     4621 AACAGGCAAA ATTACCCGCA AGGAGTTGTT GAAACAATTG TTGGAGAAGG CCGGCGGTTA
     4681 GCTTAAGCAT TTTAGACAAG TGCCTATTTA TTCAGATTGA TTTTTTATAT TGAATATGGA
     4741 TGTTGATTAC GACTTGTGTT TGAAAAAGTG TTTACGAATA AAAGAACTTT TTTTGCAACC
     4801 GTTGCTAGTT GAAGTGTAGA CTCTGAATGC GAAGGGTTGC AAATGTGCCC AGTAGAAAAA
     4861 ATATTCCATA TTTTTCTGAA CTTAATTCAA TGAAATACGA GTATTACTAC AACTGCCAAT
     4921 ATCGGAACAA TTTAACGAGA GCAAGCTATA TAAAATGAAT GTTCATTTAC ACAAGCTAGA
     4981 ACCAAAACTT GAATTAAAAT TTAACATAAC TAAATGTAAC TAGATAAAAG AGAGACATAA
     5041 CCAAACGTTC AGAAGGCAGA AGAATTCAGT TAACTCCTGC AGGAATCGAT TGGCCGGCCT
     5101 CACGTGGACG CGTTATTAAT CGTTTAAACC TACGTAACCG CGGCTTAATT AAAGCTAGCC
     5161 AGCGCTTGAG CTCTCTGCGG TGTCTACAAG GTCAGAAGTA TTTTAACTCA TCCAAAAGCC
     5221 CCAGACACCG CATTATTTCC CGGTGCATGC ATTCTTCTCT GACAAGTACC TTCTCTCTCC
     5281 ATTGGCTGGA TAAAAGAATC TGTAGGTCGC CGACATCTTG TTTGTGTCTA CATGGCCAAC
     5341 ATACTTCTCT TACAATGTAT GTGCATATGC ATATGTAAGT GGAGCGTGGT GGGAGGCTTT
     5401 ATTGTAGAGC TATTGTTCTG ACGGAGTAGG TGCAAACGGA GTCTGTTTAG GTACAGTCAC
     5461 CATCTCTGCG TATCTGTACA TTTTCACTGG AGCTAGAAGG AGCTACTCTT CGACTTCGAG
     5521 AACAGGGGAG CAACCCAGTT TGTGTTCAAG GCGATTGTTA GTTGCTCCGG CAAGATAAGG
     5581 TTACATTATT CTCTACGCTT TAGCGTTTAG GGGTACTCGG ATAAATGCCA GATGAAAAAC
     5641 GCTAGGTGCA TACAGTACAA GTCGAGCCGA CATTGTTGGA CCGCCCCGAT AATTATTGTA
     5701 TGTTGTGTTT GCTTGTAAGA CCATGATTGA TATTTATTGG GTGGTTCAGC AATCGGACTC
     5761 GTTGATTATT ATTATTATTG ACCCTCATGA TATACAATGG ATTTTATTTC TGAGCAATCG
     5821 TAGCAAAATA TTCGACGCAA TGTAGCTTGA GTTTATGACC TTAGCGGACA TCACCAGCGC
     5881 AGCTTTACAT TTTCCATTCT TTGGATTTAT TTTTCTTATC CACTAGGAGA TTAGAAGTCT
     5941 AAGTATCATG TAAGAAAGGA GATAATTGTT GTCTGCTGGA TAATAAAATT TTGCTGAGTT
     6001 AGAAATGAGA TACGAATCAG CAAGATGTCT TTGGTTGCAG CCTGTAGAGA TTTAGATTTC
     6061 GACAATCTGA CTAAAATGTA CGAAGAGAAA AATAGACACA TTTAGTCAAT ACTTCAGCGT
     6121 TAAGGGTGCT ATAGACTGTG CAACTTCGTT TCACTGCTGA CATGAAATTC CAGAGACTTA
     6181 ACATATTACA TGATTATAAA TAATTTGCGT ATAATATGTT TGGCATAAAG CGATTTCTGG
     6241 AAAAGTTGCG AAAAATTCCG AATTACTACA ACTTACCAAA AAAACAGAGG CATATTTTGC
     6301 TAAATCACCA TATCCTATAC TGAGCTCCAT CGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
     6361 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     6421 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     6481 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     6541 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     6601 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     6661 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     6721 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     6781 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     6841 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     6901 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     6961 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     7021 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     7081 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     7141 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     7201 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     7261 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC
     7321 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     7381 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     7441 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     7501 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     7561 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     7621 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     7681 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     7741 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     7801 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     7861 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     7921 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     7981 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     8041 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     8101 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC
//
