LOCUS       Exported                4741 bp ds-DNA     circular SYN 08-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Jonathan Pacheco
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 18, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    629..678
                     /label=Lyn11
                     /note="Lyn11"
     misc_feature    679..689
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      691..700
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             697..1407
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(842..860)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1110..1129
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1657..2112
                     /label=f1
                     /note="f1"
     misc_feature    1684..2112
                     /label=f1
                     /note="f1"
     misc_feature    complement(1699..2112)
                     /label=f1
                     /note="f1"
     misc_feature    1789..2095
                     /label=f1( )
                     /note="f1( )"
     promoter        2174..2202
                     /label=Bacterial
                     /note="Bacterial"
     promoter        2286..2515
                     /label=SV40early
                     /note="SV40early"
     misc_feature    2453..2588
                     /label=SV40
                     /note="SV40"
     misc_feature    2637..3431
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    3667..3685
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    4016..4659
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attccggcca ccatgggatg tataaaatca
      661 aaagggaaag acagcgcgga tccaccggtc gccaccatgg tgagcaaggg cgaggaggat
      721 aacatggcca tcattaagga gttcatgcgc ttcaaggtgc acatggaggg gtccgtgaac
      781 ggccacgtgt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      841 gccaagctga aggtgaccaa gggcggcccc ctgcccttca cctgggacat cctgagccct
      901 cagttcatgt acggctccaa tgcctacgtg aagcaccccg ccgacatccc cgactacttt
      961 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaaattcga ggacggcggc
     1021 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     1081 ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc agaagaagac catgggctgg
     1141 gaggccctct ccgagcggat gtaccccgag gacggcgccc tgaagggcga ggtcaagccc
     1201 agagtgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     1261 aagaagcccg tgcagctgcc cggcgcctac aacgtcaacc gcaagctgga catcaccagc
     1321 cacaacgagg actacaccat cgtggagcag tacgagagag ccgagggccg ccactccacc
     1381 ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     1441 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1501 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1621 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1681 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1741 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1801 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1861 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1921 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1981 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2041 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2101 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2161 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2221 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2281 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2341 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2401 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2581 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2641 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2701 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2761 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     2821 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2881 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2941 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3001 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3061 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3121 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3181 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3241 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3301 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3361 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3421 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3481 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3541 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3601 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3661 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3721 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     3781 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     3841 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     3901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4021 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4081 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4141 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4201 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4261 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4321 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4381 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4441 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4501 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4561 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4621 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4681 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     4741 t
//
