LOCUS       Exported               10791 bp ds-DNA     circular SYN 12-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2023 from SnapGene 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    281..406
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    903..1136
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1108
                     /label=insertion
     misc_feature    1650..1767
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2303..2968
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             2993..3049
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3791..4495
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    4869
                     /label=deletion
     LTR             4953..5186
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5211..5229)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6017..6121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6122..6982
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7153..7741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7300..7301
                     /label=mutation
     promoter        7987..8316
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8167..8302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9450..9515
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9645..9665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10090..10224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    10299
                     /label=deletion
     LTR             10392..234
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
       61 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      241 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      301 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      361 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      421 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      481 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      541 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
      601 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
      661 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
      721 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
      781 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      841 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      901 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      961 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1021 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1081 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1141 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1201 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1261 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1321 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1381 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1441 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1501 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     1561 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     1621 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     1681 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     1741 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     1801 tggatcgata agcttgatat cgaattccgc ggcgatatca tcatccatgg ctgggactca
     1861 aggcgctaac tgcgcgtgcg ttctggggcc cggggtgccg cggcctgggc tggggcgaag
     1921 gcgggctcgg ccggaagggg tggggtcgcc gcggctcccg ggcgcttgcg cgcacttcct
     1981 gcccgagccg ctggccgccc gagggtgtgg ccgctgcgtg cgcgcgcgcc gacccggcgc
     2041 tgtttgaacc gggcggaggc ggggctggcg cccggttggg agggggttgg ggcctggctt
     2101 cctgccgcgc gccgcgggga cgcctccgac cagtgtttgc cttttatggt aataacgcgg
     2161 ccggcccggc ttcctttgtc cccaatctgg gcgcgcgccg gcgccccctg gcggcctaag
     2221 gactcggcgc gccggaagtg gccagggcgg gggcgacctc ggctcacagc gcgcccggct
     2281 attctcgcaa ctgacaggat ccatgcccgc catgaagatc gagtgccgca tcaccggcac
     2341 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcacccccg agcagggccg
     2401 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag
     2461 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc
     2521 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga
     2581 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga
     2641 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat
     2701 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag
     2761 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca
     2821 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc
     2881 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca
     2941 cgccttcaag acccccatcg ccttcgccag atctcgagtc cccgggagcg gagctactaa
     3001 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggacctg ccggctccgg
     3061 taccgatgat gatatcgcag cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg
     3121 cttcgcgggc gacgatgccc cccgggccgt cttcccctcc atcgtggggc gccccaggca
     3181 ccaggtaggg gagctggctg ggtggggcag ccccgggagc gggcgggagg caagggcgct
     3241 ttctctgcac aggagcctcc cggtttccgg ggtgggggct gcgcccgtgc tcagggcttc
     3301 ttgtcctttc cttcccaggg cgtgatggtg ggcatgggtc agaaggattc ctatgtgggc
     3361 gacgaggccc agagcaagag aggcatcctc accctgaagt accccatcgc catggatgat
     3421 gatatcgccg cgcctcgagg ggtagtcgta ctcgtcgtcg tggggtagct aggagttcca
     3481 ggactcagtt tcccctttga gcctccttta gcgactaaag cttgaagccc cacgcatctc
     3541 gactctcgcg cacaccgccc ttgttgggct caggggcggg gcgccgcccc cggaagtact
     3601 tccccttaaa ggctggggcc tgccggaaat ggcgcagcgg cagggagggg ctcttcaccc
     3661 agtccggcag ttgaagctcg gcgctcgggt tacccctgca gcgacgcccc ctggtcccac
     3721 agataccact gctgctcccg ccctttcgct cctcggccgc gcaatgggcg gatcgggtgg
     3781 gactagtggc gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     3841 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3901 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     3961 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     4021 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     4081 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4141 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4201 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4261 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4321 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4381 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4441 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggaac
     4501 cggtgctgga agtggtacac gcgacgacga gtacgactac ctctttaaag gtgaggccat
     4561 gggctctcgc actctacaca gtcctcgttc ggggacccgg gccactcccg gtggaccctc
     4621 gtgccggcca cccctgcact gatataggcc tccctcagcc cttccttttt gtgcggttcc
     4681 gtctcctacc cagctcagcc tcttctcccc cgctcagaca ggggtcccca tcacatgccg
     4741 ctctctgagc gacctctcca taggccttcg ctggcctcag agcccctccc tgcgtgtcct
     4801 tcccctggcg gactgccttc tcccacatcg tccacgacga cgagtacgac tacccatatg
     4861 tcgagggatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     4921 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4981 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5041 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5101 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5161 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     5221 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     5281 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     5341 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     5401 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     5461 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     5521 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     5581 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     5641 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     5701 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     5761 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5821 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5881 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5941 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6001 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6061 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6361 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7921 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7981 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8041 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8101 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8161 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8221 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8281 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     8341 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     8401 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     8461 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     8521 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     8581 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     8641 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     8701 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     8761 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     8821 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     8881 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     8941 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     9001 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     9061 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     9121 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     9181 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     9241 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     9301 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     9361 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     9421 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     9481 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     9541 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     9601 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     9661 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     9721 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     9781 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     9841 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     9901 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     9961 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
    10021 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    10081 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10141 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10201 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    10261 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    10321 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10381 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
    10441 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
    10501 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
    10561 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
    10621 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
    10681 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
    10741 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg g
//
