LOCUS       Exported                9377 bp ds-DNA     circular SYN 12-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2023 from SnapGene 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    136..137
                     /label=mutation
     promoter        823..1152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1003..1138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2286..2351
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2481..2501
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2926..3060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3135
                     /label=deletion
     LTR             3228..3861
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3908..4033
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4530..4763
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4735
                     /label=insertion
     misc_feature    5277..5394
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5586
                     /label=mutation
     CDS             5886..6548
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6573..6629
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7166..7399
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7424..7442)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        8230..8334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8335..9195
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
       61 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      601 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      661 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      721 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      781 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
      841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
      901 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      961 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1081 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1141 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     1201 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     1261 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     1321 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     1381 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     1441 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     1501 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     1561 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     1621 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     1681 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     1741 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     1801 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     1861 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     1921 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     1981 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     2041 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     2101 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     2161 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     2221 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     2281 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     2341 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     2401 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     2461 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     2521 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     2581 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     2641 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     2701 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     2761 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     2821 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2881 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2941 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3001 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     3061 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     3121 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     3181 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     3241 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     3301 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     3361 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     3421 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     3481 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     3541 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     3601 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     3661 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3721 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3781 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3841 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     3901 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     3961 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     4021 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     4081 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     4141 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     4201 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     4261 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     4321 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     4381 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4441 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4501 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4561 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     4621 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     4681 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     4741 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     4801 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     4861 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     4921 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     4981 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5041 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5101 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5161 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5221 attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat ccacaatttt
     5281 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     5341 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     5401 tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga attctggtaa
     5461 tgatggcttc aacatgggca tggcatagaa cggtgatgtc gggggtgggg gttcagaact
     5521 tccattatag aaggtaatga tttagaggag aaggtggttg agaatggtgc tagtggtagt
     5581 gaacaaatcc ttcccaggat ctaggtgggc tgaggatttt tgagtctgtg acactattgt
     5641 atatccagct ttagtttctg tttaccacct tacagcagca cctaatctcc tagaggactt
     5701 agcccgtgtc acacagcaca tatttgccac accctctgta aagccctggt ttataaggtt
     5761 ctttccaccg gaagctatga cagaggaaac gtgtgggtgg ggaggggtag tgggtgaggg
     5821 acccaggttc ctgacacaga cagactacac ccagggaatg aagagcaagc gccatgtccg
     5881 gatcccccgc catgaagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg
     5941 agctggtggg cggcggagag ggcacccccg agcagggccg catgaccaac aagatgaaga
     6001 gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct
     6061 tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca
     6121 acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga
     6181 gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg
     6241 gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg
     6301 agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc
     6361 tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca
     6421 tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc
     6481 tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg
     6541 ccttcgccag atctcgagtc cccgggagcg gagctactaa cttcagcctg ctgaagcagg
     6601 ctggagacgt ggaggagaac cctggaccta tgttgaagcc atcattacca ttcacatccc
     6661 tcttattcct gcagctgccc ctgctgggag tggggctgaa cacgacaatt ctgacgccca
     6721 atgggaatga agacaccaca gctggtggga aatctgggac tggagggggc tggtgagaag
     6781 ggtggctgtg ggaaggggcc gtacagagat ctggtgcctg ccactggcca ttacaatcat
     6841 gtgggcagaa ttgaaaagtg gagtgggaag ggcaaggggg agggttccct gcctcacgct
     6901 acttcttctt tctttcttgt ttgtttgttt ctttctttct tttgaggcag ggtctcacta
     6961 tgttgcctag gctggtctca aactcctggc ctctagtgat cctcctgcct cagcctttca
     7021 aagcaccagg attacagaca tgagccacca tgttgaagcc atcattacca cctcgaggga
     7081 attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7141 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7201 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7261 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7321 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7381 gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa
     7441 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
     7501 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
     7561 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
     7621 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
     7681 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     7741 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     7801 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     7861 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     7921 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
     7981 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8041 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8101 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     8161 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9361 tcttcttgag atccttt
//
