LOCUS       pATT-BsaI-Pmag-NmagHigh 3313 bp ds-DNA     circular     19-MAY-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     265..284
                     /label="pBR322ori-F"
     primer_bind     518..535
                     /label="L4440"
     promoter        634..652
                     /label="T7 promoter"
     primer_bind     634..653
                     /label="T7"
     RBS             684..706
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             726..761
                     /label="Translation 726-761"
                     /translation="GLASGSGGSSGS"
     misc_feature    762..1211
                     /label="pMag Khammash"
     CDS             762..1211
                     /label="Translation 762-1211"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQKDTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAIDRNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     misc_feature    1215..1241
                     /label="Strong RBS"
     CDS             1242..1244
                     /label="Translation 1242-1244"
                     /translation="M"
     misc_feature    1245..1694
                     /label="mMagHigh1 recode"
     CDS             1245..1694
                     /label="Translation 1245-1694"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQKDTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAIDRNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
     CDS             1695..1720
                     /label="Translation 1695-1720"
                     /translation="GGGGSASR"
     primer_bind     complement(1786..1804)
                     /label="T7 Term"
     terminator      1800..1847
                     /label="T7 terminator"
     promoter        1953..2057
                     /label="AmpR promoter"
     CDS             2058..2918
                     /label="AmpR"
     primer_bind     complement(2276..2295)
                     /label="Amp-R"
     rep_origin      3089..364
                     /label="ori"
ORIGIN
        1 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
       61 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      421 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      481 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      541 ggaagagcgc catcagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
      601 gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata gggacaccac
      661 aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata ccatcgtctc
      721 tgtccGGTCT CGCATCGggt tctggaggct caagcggatc aCACACTCTT TACGCCCCTG
      781 GAGGATACGA CATTATGGGA TATTTGCGGC AGATTAGGAA CCGCCCAAAC CCTCAGGTCG
      841 AACTGGGGCC TGTGGACACG TCATGTGCCC TGATCCTGTG CGATCTGAAG CAAAAGGACA
      901 CTCCGATCGT CTACGCCTCG GAAGCCTTCT TGTATATGAC CGGATACAGC AATGCAGAGG
      961 TGCTCGGCAG GAACTGCAGA TTCCTGCAGT CCCCCGACGG GATGGTGAAA CCAAAGTCGA
     1021 CTCGCAAATA TGTGGACTCG AACACGATCA ACACCATGCG GAAGGCCATC GACCGGAACG
     1081 CCGAGGTCCA GGTGGAGGTG GTCAACTTTA AGAAGAACGG CCAGCGGTTC GTGAACTTTC
     1141 TGACCATGAT TCCGGTCCGG GATGAAACCG GAGAGTACAG ATACTCCATG GGATTCCAGT
     1201 GCGAAACAGA Ataagaattc attaaagagg agaaaggtac cATGCATACA CTTTACGCTC
     1261 CTGGGGGCTA CGACATCATG GGCTATTTGG ATCAGATTGG CAATCGCCCG AATCCACAGG
     1321 TTGAATTAGG GCCAGTCGAT ACGTCGTGCG CACTGATTTT GTGTGATTTA AAGCAAAAGG
     1381 ATACCCCAAT TGTTTACGCG AGTGAGGCGT TTCTGTATAT GACGGGCTAC TCAAATGCGG
     1441 AGGTACTTGG CCGCAACTGT CGCTTCTTAC AATCGCCGGA CGGCATGGTA AAGCCTAAGT
     1501 CAACTCGTAA ATACGTTGAC TCCAACACTA TCAATACAAT TCGCAAAGCG ATCGATCGCA
     1561 ACGCAGAGGT CCAGGTGGAG GTTGTTAACT TTAAGAAGAA TGGGCAACGC TTCGTGAATT
     1621 TTCTTACGAT TATTCCGGTT CGTGACGAaA CCGGCGAATA TCGTTACTCT ATGGGGTTCC
     1681 AGTGTGAAAC CGAAggtggc ggaggtagcG CGTCGAGACC agcgagagac ggttaagatc
     1741 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     1801 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     1861 ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc aaggtttctt
     1921 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2281 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     2341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2581 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2761 ccggtgagcg tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3301 ctctgctaat cct
//