LOCUS       Exported                5952 bp ds-DNA     circular SYN 30-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   RWills
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    1
                     /label=GFP tag
                     /note="GFP tag"
     misc_feature    1
                     /label=Kanamycin
                     /note="Kanamycin"
     primer_bind     519..539
                     /label=CMV-F
     primer_bind     600..634
                     /label=tagBFP insert-F(2)
     CDS             613..1314
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     primer_bind     613..634
                     /label=TagBFP-FKBP-F
     primer_bind     616..639
                     /label=TagBFP fwd 1
     primer_bind     complement(715..735)
                     /label=BFP rev seq 1
     primer_bind     1203..1225
                     /label=BFP fwd seq 1
     primer_bind     complement(1292..1314)
                     /label=TagBFP rev 1
     primer_bind     complement(1293..1331)
                     /label=tagBFP insert-R
     misc_feature    1315..1354
                     /label=MCS
                     /note="MCS"
     primer_bind     1315..1340
                     /label=BFPinsert-R
     misc_feature    1324
                     /label=5'
                     /note="5'"
     primer_bind     1354..1383
                     /label=PIP4K2B_SalICut_F
     gene            1359..2610
                     /gene="PIP4K2B"
                     /gene_synonym="PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta;
                     PIP5P4KB"
                     /label=PIP4K2B
                     /note="phosphatidylinositol-5-phosphate 4-kinase type 2
                     beta"
                     /db_xref="GeneID:8396"
                     /db_xref="HGNC:HGNC:8998"
                     /db_xref="MIM:603261"
     CDS             1360..2610
                     /codon_start=1
                     /gene="PIP4K2B"
                     /gene_synonym="PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta;
                     PIP5P4KB"
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     beta"
                     /EC_number="2.7.1.149"
                     /label=PIP4K2B
                     /note="phosphatidylinositol-4-phosphate 5-kinase, type II,
                     beta; 1-phosphatidylinositol-4-phosphate kinase; 
                     PTDINS(4)P-5-kinase; PIP4KII-beta; PI(5)P 4-kinase type II 
                     beta; diphosphoinositide kinase 2-beta; ptdIns(5)P-4-kinase
                     isoform 2-beta; 1-phosphatidylinositol 5-phosphate 4-kinase
                     2-beta; phosphatidylinositol 5-phosphate 4-kinase type II 
                     beta"
                     /db_xref="CCDS:CCDS11329.1"
                     /db_xref="GeneID:8396"
                     /db_xref="HGNC:HGNC:8998"
                     /db_xref="MIM:603261"
                     /protein_id="NP_003550.1"
                     /translation="MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSV
                     LMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFR
                     NLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNI
                     LKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTV
                     AREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMDYSL
                     LVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGTPPDSPGNLLSFPRFFGPG
                     EFDPSVDVYAMKSHESSPKKEVYFMAIIDILTPYDTKKKAAHAAKTVKHGAGAEISTVN
                     PEQYSKRFNEFMSNILT"
     primer_bind     complement(2584..2616)
                     /label=PIP4K2B_BamHICut_R
     misc_feature    2612..2638
                     /label=MCS
                     /note="MCS"
     primer_bind     complement(2646..2665)
                     /label=SV40-pArev
     primer_bind     2699..2721
                     /label=A42
     misc_feature    2868..3323
                     /label=f1
                     /note="f1"
     promoter        3385..3413
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3497..3726
                     /label=SV40early
                     /note="SV40early"
     misc_feature    3664..3799
                     /label=SV40
                     /note="SV40"
     misc_feature    3848..4642
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    4878..4896
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5227..5870
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
      661 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
      721 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
      781 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
      841 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
      901 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
      961 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     1021 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     1081 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag
     1141 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     1201 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     1261 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga
     1321 ctcagatctc gagctcaagc ttcgaattct gcagtcgaca tgtcgtccaa ctgcaccagc
     1381 accacggcgg tggcggtggc gccgctcagc gccagcaaga ccaagaccaa gaagaagcat
     1441 ttcgtgtgcc agaaagtgaa gctattccgg gccagcgagc cgatcctcag cgtcctgatg
     1501 tggggggtga accacacgat caatgagctg agcaatgttc ctgttcctgt catgctaatg
     1561 ccagatgact tcaaagccta cagcaagatc aaggtggaca atcatctctt caataaggag
     1621 aacctgccca gccgctttaa gtttaaggag tattgcccca tggtgttccg aaaccttcgg
     1681 gagaggtttg gaattgatga tcaggattac cagaattcag tgacgcgcag cgcccccatc
     1741 aacagtgaca gccagggtcg gtgtggcacg cgtttcctca ccacctacga ccggcgcttt
     1801 gtcatcaaga ctgtgtccag cgaggacgtg gcggagatgc acaacatctt aaagaaatac
     1861 caccagttta tagtggagtg tcatggcaac acgcttttgc cacagttcct gggcatgtac
     1921 cgcctgaccg tggatggtgt ggaaacctac atggtggtta ccaggaacgt gttcagccat
     1981 cggctcactg tgcatcgcaa gtatgacctc aagggttcta cggttgccag agaagcgagc
     2041 gacaaggaga aggccaagga cttgccaaca ttcaaagaca atgacttcct caatgaaggg
     2101 cagaagctgc atgtgggaga ggagagtaaa aagaacttcc tggagaaact gaagcgggac
     2161 gttgagttct tggcacagct gaagatcatg gactacagcc tgctggtggg catccacgac
     2221 gtggaccggg cagagcagga ggagatggag gtggaggagc gggcagagga cgaggagtgt
     2281 gagaatgatg gggtgggtgg caacctactc tgctcctatg gcacacctcc ggacagccct
     2341 ggcaacctcc tcagctttcc tcggttcttt ggtcctgggg aattcgaccc ctctgttgac
     2401 gtctatgcca tgaaaagcca tgaaagttcc cccaagaagg aggtgtattt catggccatc
     2461 attgatatcc tcacgccata cgatacaaag aagaaagctg cacatgctgc caaaacggtg
     2521 aaacacgggg caggggccga gatctcgact gtgaaccctg agcagtactc caaacgcttc
     2581 aacgagttta tgtccaacat cctgacgtag ggatccaccg gatctagata actgatcata
     2641 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2701 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2821 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     2881 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2941 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3001 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3061 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3121 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3181 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3241 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3301 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3421 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3481 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3781 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3841 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3901 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3961 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4021 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4081 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4141 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4201 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4261 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4321 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4381 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4441 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4501 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4561 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4621 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4681 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4741 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4801 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4861 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     4921 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4981 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5041 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5101 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5161 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5221 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5281 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5341 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5401 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5461 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5521 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5581 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5641 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5701 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5761 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5821 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5881 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5941 accgccatgc at
//
