LOCUS       Exported                5978 bp ds-DNA     circular SYN 25-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Hammond Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    1
                     /label=Kanamycin
                     /note="Kanamycin"
     misc_feature    1
                     /label=GFP tag
                     /note="GFP tag"
     primer_bind     519..539
                     /label=CMV-F
     primer_bind     600..634
                     /label=tagBFP insert-F(2)
     CDS             613..1314
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     primer_bind     613..634
                     /label=TagBFP-FKBP-F
     primer_bind     616..639
                     /label=TagBFP fwd 1
     primer_bind     complement(715..735)
                     /label=BFP rev seq 1
     primer_bind     1203..1225
                     /label=BFP fwd seq 1
     primer_bind     complement(1292..1314)
                     /label=TagBFP rev 1
     primer_bind     complement(1293..1331)
                     /label=tagBFP insert-R
     misc_feature    1315..1368
                     /label=MCS
                     /note="MCS"
     primer_bind     1315..1340
                     /label=BFPinsert-R
     misc_feature    1324
                     /label=5'
                     /note="5'"
     CDS             1369..2634
                     /codon_start=1
                     /gene="PIP4K2C"
                     /gene_synonym="PIP5K2C"
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     gamma isoform a"
                     /EC_number="2.7.1.149"
                     /label=PIP4K2C
                     /note="isoform a is encoded by transcript variant 1; 
                     phosphatidylinositol-4-phosphate 5-kinase, type II, gamma; 
                     phosphatidylinositol 4-phosphate 5-kinase; PIP4KII-gamma; 
                     PI(5)P 4-kinase type II gamma; phosphatidylinositol 
                     5-phosphate 4-kinase type II gamma"
                     /db_xref="CCDS:CCDS8946.1"
                     /db_xref="GeneID:79837"
                     /db_xref="HGNC:HGNC:23786"
                     /db_xref="MIM:617104"
                     /protein_id="NP_079055.3"
                     /translation="MASSSVPPATVSAATAGPGPGFGFASKTKKKHFVQQKVKVFRAAD
                     PLVGVFLWGVAHSINELSQVPPPVMLLPDDFKASSKIKVNNHLFHRENLPSHFKFKEYC
                     PQVFRNLRDRFGIDDQDYLVSLTRNPPSESEGSDGRFLISYDRTLVIKEVSSEDIADMH
                     SNLSNYHQYIVKCHGNTLLPQFLGMYRVSVDNEDSYMLVMRNMFSHRLPVHRKYDLKGS
                     LVSREASDKEKVKELPTLKDMDFLNKNQKVYIGEEEKKIFLEKLKRDVEFLVQLKIMKY
                     SLLLGIHDIIRGSEPEEEAPVREDESEVDGDCSLTGPPALVGSYGTSPEGIGGYIHSHR
                     PLGPGEFESFIDVYAIRSAEGAPQKEVYFMGLIDILTQYDAKKKAAHAAKTVKHGAGAE
                     ISTVHPEQYAKRFLDFITNIFA"
     primer_bind     1369..1391
                     /label=PIP4K2C_KpnICut_F
     primer_bind     complement(2181..2205)
                     /label=PIP4K2C_D280_R
     primer_bind     2206..2232
                     /label=PIP4K2C_D280K_F
     CDS             2206..2208
                     /codon_start=1
                     /label=D280K
                     /translation="K"
     primer_bind     complement(2607..2634)
                     /label=PIP4K2C_BamHICut_R
     misc_feature    2635..2664
                     /label=MCS
                     /note="MCS"
     primer_bind     2635..2653
                     /label=HsPIP5K1C_C1Swap_F
     primer_bind     complement(2672..2691)
                     /label=SV40-pArev
     primer_bind     2725..2747
                     /label=A42
     misc_feature    2894..3349
                     /label=f1
                     /note="f1"
     promoter        3411..3439
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3523..3752
                     /label=SV40early
                     /note="SV40early"
     misc_feature    3690..3825
                     /label=SV40
                     /note="SV40"
     misc_feature    3874..4668
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    4904..4922
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5253..5896
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
      661 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
      721 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
      781 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
      841 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
      901 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
      961 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     1021 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     1081 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag
     1141 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     1201 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     1261 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga
     1321 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcgat ggcgtcctcc
     1381 tcggtcccac cagccacggt atcggcggcg acagcaggcc ccggcccagg tttcggcttc
     1441 gcctccaaga ccaagaagaa gcatttcgtg cagcagaagg tgaaggtgtt ccgggcggcc
     1501 gacccgctgg tgggtgtgtt cctgtggggc gtagcccact cgatcaatga gctcagccag
     1561 gtgcctcccc cggtgatgct gctgccagat gactttaagg ccagctccaa gatcaaggtc
     1621 aacaatcacc ttttccacag ggaaaatctg cccagtcatt tcaagttcaa ggagtattgt
     1681 ccccaggtct tcaggaacct ccgtgatcga tttggcattg atgaccaaga ttacttggtg
     1741 tcccttaccc gaaacccccc cagcgaaagt gaaggcagtg atggtcgctt ccttatctcc
     1801 tacgatcgga ctctggtcat caaagaagta tccagtgagg acattgctga catgcatagc
     1861 aacctctcca actatcacca gtacattgtg aagtgccatg gcaacacgct tctgccccag
     1921 ttcctgggga tgtaccgagt cagtgtggac aacgaagaca gctacatgct tgtgatgcgc
     1981 aatatgttta gccaccgtct tcctgtgcac aggaagtatg acctcaaggg ttccctagtg
     2041 tcccgggaag ccagcgataa ggaaaaggtt aaagaattgc ccacccttaa ggatatggac
     2101 tttctcaaca agaaccagaa agtatatatt ggtgaagagg agaagaaaat atttctggag
     2161 aagctgaaga gagatgtgga gtttctagtg cagctgaaga tcatgaaata cagccttctg
     2221 ctaggcatcc acgacatcat tcggggctct gaaccagagg aggaagcgcc cgtgcgggag
     2281 gatgagtcag aggtggatgg ggactgcagc ctgactggac ctcctgctct ggtgggctcc
     2341 tatggcacct ccccagaggg tatcggaggc tacatccatt cccatcggcc cctgggccca
     2401 ggagagtttg agtccttcat tgatgtctat gccatccgga gtgctgaagg agccccccag
     2461 aaggaggtct acttcatggg cctcattgat atccttacac agtatgatgc caagaagaaa
     2521 gcagctcatg cagccaaaac tgtcaagcat ggggctgggg cagagatctc tactgtccat
     2581 ccggagcagt atgctaagcg attcctggat tttattacca acatctttgc ctaacgggat
     2641 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     2701 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2761 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2881 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2941 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3001 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3061 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3121 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3181 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3241 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3301 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3361 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3421 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3481 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3661 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3721 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3781 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     3841 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3901 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3961 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4021 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4081 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4141 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4201 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4261 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4321 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4381 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4441 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4501 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4561 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4621 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4681 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4741 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4801 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     4861 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4921 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4981 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5041 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5101 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5161 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5221 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5401 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5941 cccctgattc tgtggataac cgtattaccg ccatgcat
//
