LOCUS       Exported                6357 bp ds-DNA     circular SYN 16-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   RWills
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    613..1320
                     /label=mCherry
                     /note="mCherry"
     misc_feature    1330
                     /label=5'
                     /note="5'"
     misc_feature    1342..1368
                     /label=AAAGAGGAA
     misc_feature    1384..1701
                     /label=FKBP
                     /note="FKBP"
     misc_feature    1708..1761
                     /label=GGSAx4GG
     misc_feature    1772..1789
                     /label=MCS
                     /note="3'"
     primer_bind     complement(1774..1799)
                     /label=INPPL1_V_R
     primer_bind     1789..1818
                     /label=PIP4K2A_SalICut_F
     CDS             1795..3015
                     /codon_start=1
                     /gene="PIP4K2A"
                     /gene_synonym="PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA;
                     PIPK"
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     alpha isoform 1"
                     /EC_number="2.7.1.149"
                     /label=PIP4K2A
                     /note="isoform 1 is encoded by transcript variant 1; 
                     phosphatidylinositol-4-phosphate 5-kinase, type II, alpha; 
                     PIP5KIIalpha; PtdIns(4)P-5-kinase B isoform; type II 
                     phosphatidylinositol-4-phosphate 5-kinase 53 K isoform; 
                     1-phosphatidylinositol-4-phosphate kinase; 
                     1-phosphatidylinositol-4-phosphate-5-kinase; PIP5KIII; 
                     PIP4KII-alpha; PI(5)P 4-kinase type II alpha; 
                     ptdIns(4)P-5-kinase C isoform; diphosphoinositide kinase 
                     2-alpha; ptdIns(5)P-4-kinase isoform 2-alpha; 
                     1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; 
                     phosphatidylinositol 5-phosphate 4-kinase type II alpha"
                     /db_xref="CCDS:CCDS7141.1"
                     /db_xref="GeneID:5305"
                     /db_xref="HGNC:HGNC:8997"
                     /db_xref="MIM:603140"
                     /protein_id="NP_005019.2"
                     /translation="MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGV
                     NHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRER
                     FGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYH
                     QYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREAS
                     DKEKAKELPTLKDNDFINEGQKIYIDDNSKKVFLEKLKKDVEFLAQLKLMKYSLLVGIH
                     DVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYG
                     IKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLD
                     FIGHILT"
     primer_bind     1795..1812
                     /label=PIP4KA_Insert_F
     primer_bind     complement(1942..1969)
                     /label=PIP4K2A_VD_R
     primer_bind     1988..2019
                     /label=PIP4K2A_VD_F
     CDS             2425..2427
                     /codon_start=1
                     /label=D211
                     /translation="D"
     primer_bind     2428..2452
                     /label=PIP4K2A_D211_F
     primer_bind     complement(2592..2610)
                     /label=PIP4K2A_D273_R
     primer_bind     2611..2638
                     /label=PIP4K2A_D273K_F
     CDS             2611..2613
                     /codon_start=1
                     /label=D273K
                     /translation="K"
     primer_bind     complement(2869..2904)
                     /label=AtoE_PIP4K2Aswitch_REV
     primer_bind     2908..2938
                     /label=AtoE_PIP4K2Aswitch_FWD
     primer_bind     complement(2989..3021)
                     /label=PIP4K2A_BamHICut_R
     primer_bind     complement(2998..3015)
                     /label=PIP4KA_Insert_R
     misc_feature    3017..3052
                     /label=MCS
                     /note="3'"
     misc_feature    3273..3728
                     /label=f1
                     /note="f1"
     promoter        3790..3818
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3902..4131
                     /label=SV40early
                     /note="SV40early"
     misc_feature    4069..4204
                     /label=SV40
                     /note="SV40"
     misc_feature    4253..5047
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5283..5301
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5632..6275
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgaag cgcggccgcg ggagcaggag gagcagctcg agcggcgctg
     1381 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
     1441 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
     1501 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     1561 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     1621 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     1681 gtggagcttc taaaactgga aagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     1741 gctggtggta gtgctggtgg tccgcgagct caagcttcga attctgcagt cgacatggcg
     1801 acccccggca acctagggtc ctctgtcctg gcgagcaaga ccaagaccaa gaagaagcac
     1861 ttcgtagcgc agaaagtgaa gctgtttcgg gccagcgacc cgctgctcag cgtcctcatg
     1921 tggggggtaa accactcgat caatgaactg agccatgttc aaatccctgt tatgttgatg
     1981 ccagatgact tcaaagccta ttcaaaaata aaggtggaca atcacctttt taacaaagaa
     2041 aacatgccga gccatttcaa gtttaaggaa tactgcccga tggtcttccg taacctgcgg
     2101 gagaggtttg gaattgatga tcaagatttc cagaattccc tgaccaggag cgcacccctc
     2161 cccaacgact cccaggcccg cagtggagct cgttttcaca cttcctacga caaaagatac
     2221 atcatcaaga ctattaccag tgaagacgtg gccgaaatgc acaacatcct gaagaaatac
     2281 caccagtaca tagtggaatg tcatgggatc acccttcttc cccagttctt gggcatgtac
     2341 cggcttaatg ttgatggagt tgaaatatat gtgatagtta caagaaatgt attcagccac
     2401 cgtttgtctg tgtataggaa atacgactta aagggctcta cagtggctag agaagctagt
     2461 gacaaagaaa aggccaaaga actgccaact ctgaaagata atgatttcat taatgagggc
     2521 caaaagattt atattgatga caacagcaag aaggtcttcc tggaaaaact aaaaaaggat
     2581 gttgagtttc tggcccagct gaagctcatg aaatacagtc tgctggtggg aattcatgat
     2641 gtggagagag ccgaacagga ggaagtggag tgtgaggaga acgatgggga ggaggagggc
     2701 gagagcgatg gcacccaccc ggtgggaacc cccccagata gccccgggaa tacactgaac
     2761 agctcaccac ccctggctcc cggggagttc gatccgaaca tcgacgtcta tggaattaag
     2821 tgccatgaaa actcgcctag gaaggaggtg tacttcatgg caattattga catccttact
     2881 cattatgatg caaaaaagaa agctgcccat gctgcaaaaa ctgttaaaca tggcgctggc
     2941 gcggagatct ccaccgtgaa cccagaacag tattcaaagc gctttttgga ctttattggc
     3001 cacatcttga cgtaaggatc caccggatct agataactga tcataatcag ccataccaca
     3061 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3241 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3301 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3361 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3421 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3481 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3541 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3601 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3661 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3721 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3841 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3901 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3961 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4021 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4201 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4261 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4321 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4381 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4441 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4501 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4561 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4621 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4681 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4741 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4801 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4861 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4921 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4981 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5041 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5101 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5161 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5221 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5281 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5341 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5401 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5461 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5521 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5641 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5701 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5761 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5821 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5881 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5941 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6001 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6061 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6121 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6181 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6241 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6301 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
