LOCUS       Exported                6387 bp ds-DNA     circular SYN 03-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Hammond Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     primer_bind     519..539
                     /label=CMV-F
     primer_bind     complement(597..612)
                     /label=PLD-VR
     misc_feature    613..1320
                     /label=mCherry
                     /note="mCherry"
     primer_bind     613..630
                     /label=FPi-F
                     /note="Amplify fluorescent protein from eGFP/CFP etc pr 
                     mCherry etc"
     primer_bind     613..630
                     /label=PLD-mCh-F
     primer_bind     complement(616..633)
                     /label=FPv-R
                     /note="Amplify vector without eGFP/CFP etc pr mCherry etc. 
                     Use to exchange with FPi primers."
     primer_bind     616..633
                     /label=FPi-F2.0
     primer_bind     complement(663..681)
                     /label=mRFP-N1 (-)
     primer_bind     1175..1193
                     /label=AC41
     primer_bind     1253..1273
                     /label=mRFP-C1(+)
     primer_bind     complement(1300..1320)
                     /label=RepGFPr
     primer_bind     1301..1320
                     /label=FPv-F
                     /note="Amplify vector without eGFP/CFP etc pr mCherry etc. 
                     Use to exchange with FPi primers."
     primer_bind     complement(1301..1320)
                     /label=FPi-R
                     /note="Amplify fluorescent protein from eGFP/CFP etc pr 
                     mCherry etc"
     misc_feature    1330
                     /label=5'
                     /note="5'"
     misc_feature    1342..1368
                     /label=AAAGAGGAA
     misc_feature    1384..1701
                     /label=FKBP
                     /note="FKBP"
     misc_feature    1708..1761
                     /label=GGSAx4GG
     misc_feature    1772..1789
                     /label=MCS
                     /note="3'"
     primer_bind     1789..1818
                     /label=PIP4K2B_SalICut_F
     gene            1794..2625
                     /gene="PIP4K2B"
                     /gene_synonym="PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta;
                     PIP5P4KB"
                     /label=PIP4K2B
                     /note="phosphatidylinositol-5-phosphate 4-kinase type 2
                     beta"
                     /db_xref="GeneID:8396"
                     /db_xref="HGNC:HGNC:8998"
                     /db_xref="MIM:603261"
     CDS             1795..3045
                     /codon_start=1
                     /gene="PIP4K2B"
                     /gene_synonym="PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta;
                     PIP5P4KB"
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     beta"
                     /EC_number="2.7.1.149"
                     /label=PIP4K2B
                     /note="phosphatidylinositol-4-phosphate 5-kinase, type II,
                     beta; 1-phosphatidylinositol-4-phosphate kinase; 
                     PTDINS(4)P-5-kinase; PIP4KII-beta; PI(5)P 4-kinase type II 
                     beta; diphosphoinositide kinase 2-beta; ptdIns(5)P-4-kinase
                     isoform 2-beta; 1-phosphatidylinositol 5-phosphate 4-kinase
                     2-beta; phosphatidylinositol 5-phosphate 4-kinase type II 
                     beta"
                     /db_xref="CCDS:CCDS11329.1"
                     /db_xref="GeneID:8396"
                     /db_xref="HGNC:HGNC:8998"
                     /db_xref="MIM:603261"
                     /protein_id="NP_003550.1"
                     /translation="MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSV
                     LMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFR
                     NLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNI
                     LKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTV
                     AREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMKYSL
                     LVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGTPPDSPGNLLSFPRFFGPG
                     EFDPSVDVYAMKSHESSPKKEVYFMAIIDILTPYDTKKKAAHAAKTVKHGAGAEISTVN
                     PEQYSKRFNEFMSNILT"
     primer_bind     complement(2607..2625)
                     /label=PIP4K2B_D278_R
     primer_bind     2626..2646
                     /label=PIP4K2B_D278K_F
     gene            2629..3045
                     /gene="PIP4K2B"
                     /gene_synonym="PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta;
                     PIP5P4KB"
                     /label=PIP4K2B
                     /note="phosphatidylinositol-5-phosphate 4-kinase type 2
                     beta"
                     /db_xref="GeneID:8396"
                     /db_xref="HGNC:HGNC:8998"
                     /db_xref="MIM:603261"
     primer_bind     complement(3019..3051)
                     /label=PIP4K2B_BamHICut_R
     primer_bind     3045..3062
                     /label=HsPIP5K1C_C1Swap_F
     misc_feature    3047..3073
                     /label=MCS
                     /note="MCS"
     primer_bind     complement(3081..3100)
                     /label=SV40-pArev
     primer_bind     3134..3156
                     /label=A42
     misc_feature    3303..3758
                     /label=f1
                     /note="f1"
     promoter        3820..3848
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3932..4161
                     /label=SV40early
                     /note="SV40early"
     misc_feature    4099..4234
                     /label=SV40
                     /note="SV40"
     misc_feature    4283..5077
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5313..5331
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5662..6305
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgaag cgcggccgcg ggagcaggag gagcagctcg agcggcgctg
     1381 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
     1441 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
     1501 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     1561 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     1621 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     1681 gtggagcttc taaaactgga aagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     1741 gctggtggta gtgctggtgg tccgcgagct caagcttcga attctgcagt cgacatgtcg
     1801 tccaactgca ccagcaccac ggcggtggcg gtggcgccgc tcagcgccag caagaccaag
     1861 accaagaaga agcatttcgt gtgccagaaa gtgaagctat tccgggccag cgagccgatc
     1921 ctcagcgtcc tgatgtgggg ggtgaaccac acgatcaatg agctgagcaa tgttcctgtt
     1981 cctgtcatgc taatgccaga tgacttcaaa gcctacagca agatcaaggt ggacaatcat
     2041 ctcttcaata aggagaacct gcccagccgc tttaagttta aggagtattg ccccatggtg
     2101 ttccgaaacc ttcgggagag gtttggaatt gatgatcagg attaccagaa ttcagtgacg
     2161 cgcagcgccc ccatcaacag tgacagccag ggtcggtgtg gcacgcgttt cctcaccacc
     2221 tacgaccggc gctttgtcat caagactgtg tccagcgagg acgtggcgga gatgcacaac
     2281 atcttaaaga aataccacca gtttatagtg gagtgtcatg gcaacacgct tttgccacag
     2341 ttcctgggca tgtaccgcct gaccgtggat ggtgtggaaa cctacatggt ggttaccagg
     2401 aacgtgttca gccatcggct cactgtgcat cgcaagtatg acctcaaggg ttctacggtt
     2461 gccagagaag cgagcgacaa ggagaaggcc aaggacttgc caacattcaa agacaatgac
     2521 ttcctcaatg aagggcagaa gctgcatgtg ggagaggaga gtaaaaagaa cttcctggag
     2581 aaactgaagc gggacgttga gttcttggca cagctgaaga tcatgaaata cagcctgctg
     2641 gtgggcatcc acgacgtgga ccgggcagag caggaggaga tggaggtgga ggagcgggca
     2701 gaggacgagg agtgtgagaa tgatggggtg ggtggcaacc tactctgctc ctatggcaca
     2761 cctccggaca gccctggcaa cctcctcagc tttcctcggt tctttggtcc tggggaattc
     2821 gacccctctg ttgacgtcta tgccatgaaa agccatgaaa gttcccccaa gaaggaggtg
     2881 tatttcatgg ccatcattga tatcctcacg ccatacgata caaagaagaa agctgcacat
     2941 gctgccaaaa cggtgaaaca cggggcaggg gccgagatct cgactgtgaa ccctgagcag
     3001 tactccaaac gcttcaacga gtttatgtcc aacatcctga cgtagggatc caccggatct
     3061 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3121 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     3301 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3361 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3421 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3481 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3541 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3601 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3661 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3721 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3841 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3901 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3961 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4081 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4201 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     4261 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4321 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4381 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4441 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4501 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4561 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4621 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4681 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4741 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4801 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4861 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4921 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4981 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5041 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5101 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5161 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5221 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     5281 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     5341 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5401 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5461 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5521 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5581 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5641 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5701 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5761 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5821 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5881 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5941 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6001 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6061 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6121 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6181 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6241 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6301 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6361 gtggataacc gtattaccgc catgcat
//
