LOCUS       Exported                6413 bp ds-DNA     circular SYN 03-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Hammond Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     primer_bind     519..539
                     /label=CMV-F
     primer_bind     complement(597..612)
                     /label=PLD-VR
     misc_feature    613..1320
                     /label=mCherry
                     /note="mCherry"
     primer_bind     613..630
                     /label=FPi-F
                     /note="Amplify fluorescent protein from eGFP/CFP etc pr 
                     mCherry etc"
     primer_bind     613..630
                     /label=PLD-mCh-F
     primer_bind     complement(616..633)
                     /label=FPv-R
                     /note="Amplify vector without eGFP/CFP etc pr mCherry etc. 
                     Use to exchange with FPi primers."
     primer_bind     616..633
                     /label=FPi-F2.0
     primer_bind     complement(663..681)
                     /label=mRFP-N1 (-)
     primer_bind     1175..1193
                     /label=AC41
     primer_bind     1253..1273
                     /label=mRFP-C1(+)
     primer_bind     complement(1300..1320)
                     /label=RepGFPr
     primer_bind     1301..1320
                     /label=FPv-F
                     /note="Amplify vector without eGFP/CFP etc pr mCherry etc. 
                     Use to exchange with FPi primers."
     primer_bind     complement(1301..1320)
                     /label=FPi-R
                     /note="Amplify fluorescent protein from eGFP/CFP etc pr 
                     mCherry etc"
     misc_feature    1330
                     /label=5'
                     /note="5'"
     misc_feature    1342..1368
                     /label=AAAGAGGAA
     misc_feature    1384..1701
                     /label=FKBP
                     /note="FKBP"
     misc_feature    1708..1761
                     /label=GGSAx4GG
     misc_feature    1772..1789
                     /label=MCS
                     /note="3'"
     misc_feature    1790..1803
                     /label=MCS
                     /note="MCS"
     CDS             1804..3069
                     /codon_start=1
                     /gene="PIP4K2C"
                     /gene_synonym="PIP5K2C"
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     gamma isoform a"
                     /EC_number="2.7.1.149"
                     /label=PIP4K2C
                     /note="isoform a is encoded by transcript variant 1; 
                     phosphatidylinositol-4-phosphate 5-kinase, type II, gamma; 
                     phosphatidylinositol 4-phosphate 5-kinase; PIP4KII-gamma; 
                     PI(5)P 4-kinase type II gamma; phosphatidylinositol 
                     5-phosphate 4-kinase type II gamma"
                     /db_xref="CCDS:CCDS8946.1"
                     /db_xref="GeneID:79837"
                     /db_xref="HGNC:HGNC:23786"
                     /db_xref="MIM:617104"
                     /protein_id="NP_079055.3"
                     /translation="MASSSVPPATVSAATAGPGPGFGFASKTKKKHFVQQKVKVFRAAD
                     PLVGVFLWGVAHSINELSQVPPPVMLLPDDFKASSKIKVNNHLFHRENLPSHFKFKEYC
                     PQVFRNLRDRFGIDDQDYLVSLTRNPPSESEGSDGRFLISYDRTLVIKEVSSEDIADMH
                     SNLSNYHQYIVKCHGNTLLPQFLGMYRVSVDNEDSYMLVMRNMFSHRLPVHRKYDLKGS
                     LVSREASDKEKVKELPTLKDMDFLNKNQKVYIGEEEKKIFLEKLKRDVEFLVQLKIMKY
                     SLLLGIHDIIRGSEPEEEAPVREDESEVDGDCSLTGPPALVGSYGTSPEGIGGYIHSHR
                     PLGPGEFESFIDVYAIRSAEGAPQKEVYFMGLIDILTQYDAKKKAAHAAKTVKHGAGAE
                     ISTVHPEQYAKRFLDFITNIFA"
     primer_bind     1804..1826
                     /label=PIP4K2C_KpnICut_F
     primer_bind     complement(2616..2640)
                     /label=PIP4K2C_D280_R
     primer_bind     2641..2667
                     /label=PIP4K2C_D280K_F
     CDS             2641..2643
                     /codon_start=1
                     /label=D280K
                     /translation="K"
     primer_bind     complement(3042..3069)
                     /label=PIP4K2C_BamHICut_R
     misc_feature    3070..3099
                     /label=MCS
                     /note="MCS"
     primer_bind     3070..3088
                     /label=HsPIP5K1C_C1Swap_F
     primer_bind     complement(3107..3126)
                     /label=SV40-pArev
     primer_bind     3160..3182
                     /label=A42
     misc_feature    3329..3784
                     /label=f1
                     /note="f1"
     promoter        3846..3874
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3958..4187
                     /label=SV40early
                     /note="SV40early"
     misc_feature    4125..4260
                     /label=SV40
                     /note="SV40"
     misc_feature    4309..5103
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5339..5357
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5688..6331
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgaag cgcggccgcg ggagcaggag gagcagctcg agcggcgctg
     1381 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
     1441 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
     1501 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     1561 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     1621 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     1681 gtggagcttc taaaactgga aagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     1741 gctggtggta gtgctggtgg tccgcgagct caagcttcga attctgcagt cgacggtacc
     1801 gcgatggcgt cctcctcggt cccaccagcc acggtatcgg cggcgacagc aggccccggc
     1861 ccaggtttcg gcttcgcctc caagaccaag aagaagcatt tcgtgcagca gaaggtgaag
     1921 gtgttccggg cggccgaccc gctggtgggt gtgttcctgt ggggcgtagc ccactcgatc
     1981 aatgagctca gccaggtgcc tcccccggtg atgctgctgc cagatgactt taaggccagc
     2041 tccaagatca aggtcaacaa tcaccttttc cacagggaaa atctgcccag tcatttcaag
     2101 ttcaaggagt attgtcccca ggtcttcagg aacctccgtg atcgatttgg cattgatgac
     2161 caagattact tggtgtccct tacccgaaac ccccccagcg aaagtgaagg cagtgatggt
     2221 cgcttcctta tctcctacga tcggactctg gtcatcaaag aagtatccag tgaggacatt
     2281 gctgacatgc atagcaacct ctccaactat caccagtaca ttgtgaagtg ccatggcaac
     2341 acgcttctgc cccagttcct ggggatgtac cgagtcagtg tggacaacga agacagctac
     2401 atgcttgtga tgcgcaatat gtttagccac cgtcttcctg tgcacaggaa gtatgacctc
     2461 aagggttccc tagtgtcccg ggaagccagc gataaggaaa aggttaaaga attgcccacc
     2521 cttaaggata tggactttct caacaagaac cagaaagtat atattggtga agaggagaag
     2581 aaaatatttc tggagaagct gaagagagat gtggagtttc tagtgcagct gaagatcatg
     2641 aaatacagcc ttctgctagg catccacgac atcattcggg gctctgaacc agaggaggaa
     2701 gcgcccgtgc gggaggatga gtcagaggtg gatggggact gcagcctgac tggacctcct
     2761 gctctggtgg gctcctatgg cacctcccca gagggtatcg gaggctacat ccattcccat
     2821 cggcccctgg gcccaggaga gtttgagtcc ttcattgatg tctatgccat ccggagtgct
     2881 gaaggagccc cccagaagga ggtctacttc atgggcctca ttgatatcct tacacagtat
     2941 gatgccaaga agaaagcagc tcatgcagcc aaaactgtca agcatggggc tggggcagag
     3001 atctctactg tccatccgga gcagtatgct aagcgattcc tggattttat taccaacatc
     3061 tttgcctaac gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     3121 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3181 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3301 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     3361 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3421 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3481 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3541 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3601 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3661 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3721 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3781 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3901 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4021 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4081 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4201 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4261 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4321 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4381 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4441 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4501 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4561 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4621 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4681 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4741 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4801 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4861 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4921 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4981 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5041 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5101 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5161 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5221 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     5281 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     5341 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5401 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5461 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5521 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5821 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
