LOCUS       Exported                6252 bp ds-DNA     circular SYN 15-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   RWills
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     primer_bind     complement(597..636)
                     /label=Cherry delrev 1
     CDS             616..1314
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     616..639
                     /label=Fragment.FOR
     primer_bind     616..639
                     /label=TagBFP fwd 1
     primer_bind     complement(1292..1314)
                     /label=Fragment.REV
     primer_bind     complement(1292..1314)
                     /label=TagBFP rev 1
     primer_bind     1297..1335
                     /label=Cherry delfwd 1
     misc_feature    1324
                     /label=5'
                     /note="5'"
     misc_feature    1336..1362
                     /label=AAAGAGGAA
     misc_feature    1378..1695
                     /label=FKBP
                     /note="FKBP"
     primer_bind     1658..1680
                     /label=FKBP-F
     misc_feature    1702..1755
                     /label=GGSAx4GG
     primer_bind     complement(1765..1786)
                     /label=BFP-FKBP_vR
     misc_feature    1766..1809
                     /label=MCS
                     /note="3'"
     CDS             1810..2907
                     /codon_start=1
                     /label=PIPK
                     /translation="LKGAIQLGIGYTVGHLSSKPERRVLMQDFYVVESIFFPSEGSNLT
                     PAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSLFYVTSD
                     DEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNI
                     LPRVVKMHLKFDLKGSTYKRRASKKEKEKSFPTYKDLDFMQDMPEGLLLDADTFSALVK
                     TLQDDCLVLESFKIMKYSLLLGVHNIDQHERERQAQGAQSTSDEKRPVGQKALYSTAME
                     SIQGGAARGEAIESDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVH
                     DGDTVSVHRPSFYAERFFKFMSNTVF"
     misc_feature    1810..2907
                     /label=PIP5K1C
                     /note="untitled_14"
     primer_bind     1810..1830
                     /label=PIPK-F
     primer_bind     2503..2541
                     /label=PIP5K-D316K-F
     primer_bind     complement(2503..2541)
                     /label=PIP5K-D316K-R
     primer_bind     complement(2889..2911)
                     /label=PIPK-R
     primer_bind     2894..2927
                     /label=BFP-FKBP_vF-Kinase
     misc_feature    2911..2947
                     /label=MCS
                     /note="3'"
     primer_bind     2911..2928
                     /label=BFP-FKBP_vF
     primer_bind     2911..2927
                     /label=BFP-FKBP_vF-CTerm
     primer_bind     complement(3077..3096)
                     /label=GeneWiz-SV40pA-R
     misc_feature    3168..3623
                     /label=f1
                     /note="f1"
     promoter        3685..3713
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3797..4026
                     /label=SV40early
                     /note="SV40early"
     misc_feature    3964..4099
                     /label=SV40
                     /note="SV40"
     misc_feature    4148..4942
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5178..5196
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5527..6170
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
      661 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
      721 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
      781 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
      841 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
      901 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
      961 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     1021 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     1081 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag
     1141 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     1201 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     1261 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga
     1321 ctcagatctc gaagcgcggc cgcgggagca ggaggagcag ctcgagcggc gctgggagtg
     1381 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc
     1441 gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga
     1501 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg
     1561 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat
     1621 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag
     1681 cttctaaaac tggaaagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt
     1741 ggtagtgctg gtggtccgcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     1801 ccgggatccc tgaagggtgc catccagctg ggcatcggct acaccgtggg ccacctgagc
     1861 tccaagcccg aacgccgcgt gctcatgcag gacttctacg tggtggagag catcttcttc
     1921 cccagcgaag gcagcaacct cacccccgcc caccacttcc aggacttccg cttcaagacc
     1981 tatgcacctg tcgccttccg ctacttccgg gagctctttg ggatccggcc agatgattac
     2041 ttgtactccc tgtgcaatga gccgctgatc gagctgtcca acccgggcgc cagtggctcc
     2101 ctcttctacg tcaccagcga cgacgagttc atcatcaaga ccgtcatgca caaggaggcc
     2161 gagttcctgc agaagctgct ccctggctac tacatgaacc tcaaccagaa cccgcggacg
     2221 ctgctgccca agttctatgg gctgtactgc gtgcagtcgg ggggcaagaa catccgcgtc
     2281 gtggtcatga acaacatcct gccccgcgtg gtcaagatgc acctcaagtt cgacctcaag
     2341 ggctccacct acaagcggcg cgccagcaag aaggagaagg agaagagctt ccccacctac
     2401 aaggacctgg acttcatgca ggacatgccc gaggggctcc tgctggacgc cgacaccttc
     2461 agcgccctgg tcaagacgct gcaggacgac tgcctggtcc tggaaagttt caagatcatg
     2521 aaatacagcc tgctgctggg cgtgcacaac atcgaccagc acgagcgcga gcggcaggcg
     2581 cagggcgccc agagcacctc agatgagaag cggcctgtgg gccagaaggc gctctactcc
     2641 acggccatgg agtccatcca gggtggcgcc gcgcgcgggg aggccatcga atcggatgac
     2701 acgatgggcg ggatccccgc tgtgaacggc cgcggggagc ggctgctgct gcacattggc
     2761 atcatcgaca tcctgcagtc ctacaggttc atcaagaaac tggagcacac ctggaaggcc
     2821 ctcgtccacg atggggacac ggtgtccgtc caccgcccca gcttctatgc cgagcgcttt
     2881 ttcaagttca tgagcaacac ggtcttttaa ggatccaccg gatctagata actgatcata
     2941 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3001 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3061 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3121 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     3181 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3241 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3301 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3361 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3421 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3481 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3541 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3601 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3721 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3781 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3841 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3901 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3961 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4021 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4081 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4141 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4201 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4261 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4321 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4381 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4441 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4501 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4561 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4621 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4681 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4741 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4801 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4861 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4921 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4981 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5041 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5101 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5161 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5221 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5281 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5341 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5401 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5461 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5521 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5881 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5941 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6001 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6061 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6181 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6241 accgccatgc at
//
