LOCUS       P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2       11289 bp    DNA     circular UNA 23-FEB-2024
DEFINITION  RGC-iN - NEUROG2 - p2A- ATOH7 - p2A - ISLET1 - p2A - POU4F2 /
            BRN3B.
ACCESSION   urn.local...7i-gaig1vo
VERSION     urn.local...7i-gaig1vo
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    5..100
                     /note="Geneious type: U6 Promoter"
                     /standard_name="dominant control element"
     misc_feature    183..208
                     /note="Geneious type: U6 Promoter"
                     /standard_name="Pol II control element"
     misc_feature    243..262
                     /note="Geneious type: gRNA target"
                     /standard_name="CLYBLgRNAT1"
     misc_feature    263..354
                     /note="Geneious type: guide RNA scaffold"
                     /standard_name="gRNA scaffold V3"
     misc_feature    264..302
                     /standard_name="stem loop 1"
     misc_feature    275..279
                     /note="Geneious type: motif"
                     /standard_name="+5F"
     misc_feature    284..288
                     /note="Geneious type: motif"
                     /standard_name="-5R"
     misc_feature    314
                     /note="Geneious type: misc"
                     /standard_name="+48"
     misc_feature    321..332
                     /standard_name="stem loop 2"
     misc_feature    323
                     /note="Geneious type: misc"
                     /standard_name="+54"
     misc_feature    333
                     /note="Geneious type: misc"
                     /standard_name="+54"
     misc_feature    334..348
                     /standard_name="stem loop3"
     misc_feature    351
                     /note="Geneious type: misc"
                     /standard_name="+85"
     misc_feature    358..361
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    366..403
                     /note="Geneious type: recombination site"
                     /standard_name="attP"
     misc_feature    408..425
                     /note="Geneious type: endonuclease cute site"
                     /standard_name="Endonuclease cute site"
     rep_origin      complement(620..1208)
     misc_RNA        complement(620..1172)
                     /note="RNAII transcript (complementary strand)"
                     /standard_name="RNA II"
     misc_RNA        1062..1169
                     /note="Geneious type: misc_rna"
                     /note="RNAI transcript"
                     /standard_name="RNAI transcript"
     misc_feature    complement(1379..2239)
                     /note="Geneious type: ORF"
                     /standard_name="ORF 3 (frame 3)"
     sig_peptide     complement(2171..2239)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
                     /standard_name="bla signal peptide"
     regulatory      complement(2240..2331)
                     /note="Geneious type: promoter"
                     /gene="<html><body><i>bla</i></body></html>"
                     /standard_name="AmpR promoter"
     regulatory      complement(2281..2309)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    2584..2601
                     /note="Geneious type: endonuclease cute site"
                     /standard_name="Endonuclease cute site"
     misc_feature    2606..2643
                     /note="Geneious type: GFP"
                     /standard_name="attB"
     misc_feature    2693..3417
                     /note="Geneious type: Homology Arm"
                     /standard_name="CLYBL Left arm"
     misc_feature    3394..3395
                     /note="Geneious type: SNP"
                     /standard_name="SNP"
     regulatory      3418..4096
                     /standard_name="CBX3-UCOE"
     misc_feature    4097..4102
                     /note="Geneious type: endonuclease cute site"
                     /standard_name="MluI site"
     regulatory      4109..4390
                     /note="Geneious type: enhancer"
                     /standard_name="CAG enhancer"
     regulatory      4109..4864
                     /note="Geneious type: promoter"
                     /standard_name="CBh promoter"
     regulatory      4401..4632
                     /note="Geneious type: promoter"
                     /standard_name="cBA prom"
     misc_feature    4871..5242
                     /note="Geneious type: Marker"
                     /standard_name="bleo marker"
     misc_feature    4871..6073
                     /note="Geneious type: ORF"
                     /standard_name="ORF 5 (frame 2)"
     misc_feature    5243..5308
                     /note="Geneious type: P2A"
                     /standard_name="P2A"
     misc_feature    5309..6016
                     /note="Geneious type: BFP"
                     /standard_name="mTagBFP"
     misc_feature    5816..5818
                     /note="Geneious type: mutation"
                     /standard_name="G316W"
     misc_feature    6017..6064
                     /note="Geneious type: HA tag"
                     /standard_name="nucleoplasmin bipartite cNLS"
     polyA_site      6081..6210
     protein_bind    6288..6306
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b; tet operator"
                     /standard_name="tetO protein bind"
     protein_bind    6324..6342
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b; tet operator"
                     /standard_name="tetO protein bind"
     protein_bind    6360..6378
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b; tet operator"
                     /standard_name="tetO protein bind"
     protein_bind    6396..6414
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b; tet operator"
                     /standard_name="tetO protein bind"
     protein_bind    6432..6450
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b; tet operator"
                     /standard_name="tetO protein bind"
     protein_bind    6468..6486
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b; tet operator"
                     /standard_name="tetO protein bind"
     regulatory      6475..6555
                     /note="Geneious type: Promoter"
                     /standard_name="CMV prom"
     misc_feature    6556..6561
                     /note="Geneious type: kozack"
                     /standard_name="Kozak"
     CDS             6562..7377
                     /standard_name="NEUROG2 CDS"
     misc_feature    6562..10311
                     /note="Geneious type: ORF"
                     /standard_name="ORF 2 (frame 1)"
     misc_feature    7378..7443
                     /note="Geneious type: P2A"
                     /standard_name="P2A_a"
     CDS             7444..7899
                     /standard_name="ATOH7 CDS"
     misc_feature    7900..7965
                     /note="Geneious type: P2A"
                     /standard_name="P2A_b"
     CDS             7966..9012
                     /standard_name="ISL1 CDS"
     misc_feature    9013..9078
                     /note="Geneious type: P2A"
                     /standard_name="P2A_c"
     CDS             9079..10308
                     /standard_name="BRN3B CDS"
     regulatory      10343..10570
                     /note="Geneious type: Terminator"
                     /standard_name="bGH_PA term"
     misc_feature    10571..11161
                     /note="Geneious type: Homology Arm"
                     /note="Derived using Geneious Prime 2023.1.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CLYBL_Right arm"
     misc_feature    complement(11162..11184)
                     /note="Geneious type: gRNA target"
                     /standard_name="CLYBLgRNA_T1"
     misc_feature    11205..11245
                     /note="Geneious type: DTS"
                     /standard_name="eHRE"
     misc_feature    11269..11272
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      11288..242
                     /note="Geneious type: promoter"
                     /standard_name="U6 promoter (Kunkel, 1998)"
ORIGIN      
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cggaccatac tatctagaaa tagtttaaga gctaagctgg aaacagcata gcaagtttaa
      301 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttggcggc
      361 cgcgacccca actggggtaa cctttgagtt ctctcagttg gggtccatag ggataacagg
      421 gtaatttgat atcgcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      481 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      661 gacgcacaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1381 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1441 ttgcctgact cccggtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1501 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1561 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1621 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1681 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1741 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1801 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1861 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1921 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1981 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2041 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2101 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2161 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2221 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2281 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2341 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     2401 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2461 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2521 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2581 tcgtagggat aacagggtaa ttcatgggtg ccagggcgtg cccttgggct ccccgggcgc
     2641 gtaaagcttc gatgtacggg ccagatatag gaccatacta tctagaaata tggagaccag
     2701 caggagaatc atgccaatgg gccaatatac attctgaccc acagtttcat aataaaataa
     2761 aatggttgtg gttgtaagcc actatgtttc agagtggttt gttacacagc aataaataac
     2821 taatatagta ggcataccat caagtccaaa gtaggtagag aagaatgtaa atagcagagc
     2881 aaaacagcat gactggtggc tgggaggctt aaaactggga caggatcaga gtcatgaaag
     2941 aagtcaaaga aatggttcag aagtaaggct gagactgact tacaaaagct gaaagtccct
     3001 ttaagttggt gtttggtgca ttggcagggg caggtatggt gacttaaaag agccatgctc
     3061 aacaagatca agcacaacac aatcacgggt caccccagca gaccttagcg agtctagcca
     3121 tttctttggt ggtggtcaca gtcatgcttc agcccagttt ccacttggac aaatggtaca
     3181 tattttcaat gagatgaaaa ttaagataca atccatgtgc tcagagagtg atcacagctc
     3241 tgactaaaca ctgtgcccca aagtgttgag gaattgggaa aacctagctg agttagtggt
     3301 ctcttttctg ttacaataaa gctcataatg aaaattagcc ttctttgttc ttccccaagt
     3361 ctttctttct agacgaaact actttcaact gttggaactt ccttactgtt aacttccccc
     3421 gggaggtggt ccctgcagtt acgccaatga taacccccgc cagaaaaatc ttagtagcct
     3481 tccctttttg ttttccgtgc cccaactcgg cggattgact cggccccttc cggaaacacc
     3541 cgaatcaact tctagtcaaa ttattgttca cgccgcaatg acccacccct ggcccgcgtc
     3601 tgtggaactg acccctggtg tacaggagag ttcgctgctg aaagtggtcc caaaggggta
     3661 ctagttttta agctcccaac tccccctccc ccagcgtctg gaggattcca caccctcgca
     3721 ccgcaggggc gaggaagtgg gcggagtccg gttttggcgc cagccgctga ggctgccaag
     3781 cagaaaagcc accgctgagg agactccggt cactgtcctc gccccgcctc ccccttccct
     3841 ccccttgggg accaccgggc gccacgccgc gaacggtaag tgccgcggtc gtcggcgcct
     3901 ccgccctccc cctagggccc caattcccag cgggcgcggc gcgcggcccc tccccccgcc
     3961 gggcgcgcgc ccgctgcccc gcccttcgtg gccgcccggc gtgggcggtg ccacccctcc
     4021 ccccggcggc cccgcgcgca gctcccggct ccctccccct tcggatgtgg cttgagctgt
     4081 aggcgcggag ggccggacgc gtgctctaga ggtacccgtt acataactta cggtaaatgg
     4141 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaatagtaa cgccaatagg
     4201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4321 ctggcattgt gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4381 attattcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     4441 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     4501 gatgggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     4561 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     4621 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     4681 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     4741 gcccttctcc tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg
     4801 ttggtggggt attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg
     4861 ttgggccgcc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     4921 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     4981 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     5041 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     5101 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     5161 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     5221 cttcgtggcc gaggagcagg acggaagtgg agctactaac ttcagcctac ttaagcaggc
     5281 tggagacgtg gaagagaacc ctggacctgt gtctaagggc gaagagctga ttaaggagaa
     5341 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     5401 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     5461 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     5521 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     5581 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     5641 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     5701 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     5761 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggctggag
     5821 ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa
     5881 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     5941 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     6001 ggggcacaag cttaataaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     6061 aaaggaattc taactagagc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6121 caaagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6181 tccaaactca tcaatgtatc ttatcatgtc ggggattctc ctaaatcaag atagaatgtg
     6241 gcttgagctg taggcgcgga gggccggacg cgtgctctag agtttactcc ctatcagtga
     6301 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
     6361 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
     6421 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
     6481 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
     6541 gtgaaccgtc agatcgccac catgttcgtc aaatccgaga ccttggagtt gaaggaggaa
     6601 gaggacgtgt tagtgctgct cggatcggcc tcccccgcct tggcggccct gaccccgctg
     6661 tcatccagcg ccgacgaaga agaggaggag gagccgggcg cgtcaggcgg ggcgcgtcgg
     6721 cagcgcgggg ctgaggccgg gcagggggcg cggggcggcg tggctgcggg tgcggagggc
     6781 tgccggcccg cacggctgct gggtctggta cacgattgca aacggcgccc ttcccgggcg
     6841 cgggccgtct cccgaggcgc caagacggcc gagacggtgc agcgcatcaa gaagacccgt
     6901 agactgaagg ccaacaaccg cgagcgaaac cgcatgcaca acctcaacgc ggcactggac
     6961 gcgctgcgcg aggtgctccc cacgttcccc gaggacgcca agctcaccaa gatcgagacc
     7021 ctgcgcttcg cccacaacta catctgggca ctcaccgaga ccctgcgcct ggcggatcac
     7081 tgcgggggcg gcggcggggg cctgccgggg gcgctcttct ccgaggcagt gttgctgagc
     7141 ccgggaggcg ccagcgccgc cctgagcagc agcggagaca gcccctcgcc cgcctccacg
     7201 tggagttgca ccaacagccc cgcgccgtcc tcctccgtgt cctccaattc cacctccccc
     7261 tacagctgca ctttatcgcc cgccagcccg gccgggtcag acatggacta ttggcagccc
     7321 ccacctcccg acaagcaccg ctatgcacct cacctcccca tagccaggga ttgtatcggt
     7381 agcggtgcca ccaacttcag cctgctgaag caggctggag atgtggagga gaaccctgga
     7441 cctatgaagt cctgcaagcc cagcggcccg ccggcgggag cgcgcgttgc acccccgtgc
     7501 gcgggcggca ccgagtgcgc gggcacgtgc gccggggccg ggcggctgga gagcgcggcg
     7561 cgcaggcgcc tggcggccaa cgcgcgcgag cgccgccgca tgcaggggct caacactgcc
     7621 ttcgaccgct tacgcagggt ggttccccag tggggccagg ataaaaagct gtccaagtac
     7681 gagaccctgc agatggccct gagctacatc atggctctga cccggatcct ggccgaggcc
     7741 gagcgattcg gctcggagcg ggactgggtg ggtctccact gtgagcactt cggccgcgac
     7801 cactacctcc cgttcccggg cgcgaagctg ccgggcgaga gcgagctgta cagccagaga
     7861 ctcttcggct tccagcccga gcccttccag atggccaccg gaagcggagc tactaacttc
     7921 agcctgctga agcaggccgg agacgttgaa gagaatccag gacctatggg agacatggga
     7981 gatccaccaa aaaaaaaacg tctgatttcc ctatgtgttg gttgcggcaa tcagattcac
     8041 gatcagtata ttctgagggt ttctccggat ttggaatggc atgcggcatg tttgaaatgt
     8101 gcggagtgta atcagtattt ggacgagagc tgtacatgct ttgttaggga tgggaaaacc
     8161 tactgtaaaa gagattatat caggttgtac gggatcaaat gcgccaagtg cagcatcggc
     8221 ttcagcaaga acgacttcgt gatgcgtgcc cgctccaagg tgtatcacat cgagtgtttc
     8281 cgctgtgtgg cctgcagccg ccagctcatc cctggggacg aatttgcgct tcgggaggac
     8341 ggtctcttct gccgagcaga ccacgatgtg gtggagaggg ccagtctagg cgctggcgac
     8401 ccgctcagtc ccctgcatcc agcgcggcca ctgcaaatgg cagcggagcc catctccgcc
     8461 aggcagccag ccctgcggcc ccacgtccac aagcagccgg agaagaccac ccgcgtgcgg
     8521 actgtgctga acgagaagca gctgcacacc ttgcggacct gctacgccgc aaacccgcgg
     8581 ccagatgcgc tcatgaagga gcaactggta gagatgacgg gcctcagtcc ccgtgtgatc
     8641 cgggtctggt ttcaaaacaa gcggtgcaag gacaagaagc gaagcatcat gatgaagcaa
     8701 ctccagcagc agcagcccaa tgacaaaact aatatccagg ggatgacagg aactcccatg
     8761 gtggctgcca gtccagagag acacgacggt ggcttacagg ctaacccagt ggaagtacaa
     8821 agttaccagc caccttggaa agtactgagc gacttcgcct tgcagagtga catagatcag
     8881 cctgcttttc agcaactggt caatttttca gaaggaggac cgggctctaa ttccactggc
     8941 agtgaagtag catcaatgtc ctctcaactt ccagatacac ctaacagcat ggtagccagt
     9001 cctattgagg caggaagcgg agctactaat tttagtctgc tgaagcaggc tggtgacgtg
     9061 gaagagaacc ctggacctat gatgatgatg tccctgaaca gcaagcaggc gtttagcatg
     9121 ccgcacggcg gcagcctgca cgtggagccc aagtactcgg cactgcacag cacctcgccg
     9181 ggctcctcgg ctcccatcgc gccctcggcc agctccccca gcagctcgag caacgctggt
     9241 ggtggcggcg gcggcggcgg cggcggcggc ggcggcggcg gaggccgaag cagcagctcc
     9301 agcagcagtg gcagcagcgg cggcgggggc tcggaggcta tgcggagagc ctgtcttcca
     9361 accccaccga gcaatatatt cggcgggctg gatgagagtc tgctggcccg cgccgaggct
     9421 ctggcagccg tggacatcgt ctcccagagc aagagccacc accaccatcc accccaccac
     9481 agccccttca aaccggacgc cacctaccac actatgaata ccatcccgtg cacgtcggcc
     9541 gcctcttctt catcggtgcc catctcgcac ccttccgcgt tggcgggcac gcaccaccac
     9601 caccaccatc accaccacca ccaccaccaa ccgcaccagg cgctggaggg cgagctgctg
     9661 gagcacctga gtcccgggct ggccctgggc gctatggcgg gccccgacgg cgctgtggtg
     9721 tccacgccgg ctcacgcgcc gcacatggcc accatgaacc ccatgcacca agcagcgctc
     9781 agcatggccc acgcgcacgg gctgccgtcg cacatgggct gcatgagcga cgtggacgcc
     9841 gacccgcggg acctggaggc attcgccgag cgcttcaagc agcgacgcat caagctgggg
     9901 gtgacccagg cagatgtggg ctccgcgctg gccaacctca agatccccgg cgtgggctcg
     9961 cttagccaga gcaccatctg caggttcgag tccctcacac tgtcccacaa taatatgatc
    10021 gcgctcaaac ccatcctgca ggcatggctc gaggaggccg agaagtccca ccgcgagaag
    10081 ctcaccaagc ctgaactctt caatggcgcg gagaagaagc gcaagcgcac gtccatcgct
    10141 gcgccagaga agcgctcgct cgaagcctac tttgccattc agcctcggcc ctcctctgaa
    10201 aagatcgccg ccatcgcgga gaagctggac ctgaagaaaa acgtggtgcg cgtctggttc
    10261 tgcaaccaga ggcagaaaca gaaaagaatg aaatattccg ccggcattta ggaattctaa
    10321 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    10381 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    10441 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    10501 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    10561 ggctctatgg ctagatagta tggtcagact gttatttctt gacttttaaa tgtaagatat
    10621 tatctactga cttccttcta tgtaagatga ggattgagct ctcttaccct tctcccattt
    10681 cctcatcctt ccaacataaa tatattttgg gattatatca acattcaatg ttacttaaag
    10741 tgaccttgta aatattttca caactgagcc atgtttgatt tgtatactta tgtttacttt
    10801 actgtttttc ctgaagttaa taattgcctt gaatttattt atttctttaa aaatgtttca
    10861 ttactcagga ctgtagttta cattacgatt ctttgtgtta tacagttgat gggtttcttt
    10921 tctttcttaa tttctttaaa aaatagagat ggggtcttac tatattaccc aggctggtct
    10981 tgaagtcctg ggctcaagtg atcttcctgt ctcagcctac caagtagctg agactatagg
    11041 tgcaaaaaag ccactatacc tggctagttt acaggtttta acaaatgcat tatgccacgt
    11101 atccattatt acaggatcac acaagatatt ttcattaccc tgaaagcatc cctgtgttcc
    11161 accatatttc tagatagtat ggtctttggt accaagcttt gcgcggatgt atgtgtgtgc
    11221 gtgtacgtgt gtatgtgaag ataccgccta ggcgcctagg gaattcgcgg ccgccttttg
    11281 ctcacatgt
//
