LOCUS       WR23                    7936 bp ds-DNA     circular     22-MAY-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     ARS confirmed orientation
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(6943..7800)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2834..4462
                     /locus_tag="PsCO CBG99"
                     /label="PsCO CBG99"
                     /ApEinfo_label="PsCO CBG99"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6125..6792
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1617..1992
                     /locus_tag="Act term"
                     /label="Act term"
                     /ApEinfo_label="Act term"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2073..2827
                     /locus_tag="Ptdh3"
                     /label="Ptdh3"
                     /ApEinfo_label="Ptdh3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4468..4842
                     /locus_tag="Adh2term"
                     /label="Adh2term"
                     /ApEinfo_label="Adh2term"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        45..770
                     /locus_tag="Ps TEF1 promoter"
                     /label="Ps TEF1 promoter"
                     /ApEinfo_label="Ps TEF1 promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             777..1583
                     /locus_tag="PsCOkanMX"
                     /label="PsCOkanMX"
                     /ApEinfo_label="PsCOkanMX"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4955..6102)
                     /locus_tag="Ps ARS from Chsome1"
                     /label="Ps ARS from Chsome1"
                     /ApEinfo_label="Ps ARS from Chsome1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CCAATGATAC CGGTGCGATC GTGGGCCCTA GTACTTCCCT CGAGCCGTAC ACTTATTGTT
       61 AACTATGAAA CATATACTTA TGGACAAGGT TCACTGATTT GGACTGAAAA GCAAAAACGA
      121 GAAATGAGTC AACGTCTGAG ATCAATCCCT AGACCACCCG GGAAGGTCAT GTGTTTCATG
      181 AAGTACGATA AGGTTGGTAA CCGATTGACT CATTGGTTCG TGGCGGAGAA GTACGCAGAG
      241 TAAAACCGGG GCCGATTCGT GGTAAATTCT GGAATGATCC AGAGGCGCGA CATTTATGCA
      301 GACAATTTGT GTTTTGTCGC AAACGATGTT ATAGCGAAAT TTTTCACTCT GTCAGATAAA
      361 TGGATTTTGT CAAAAGGGGG AAGTAGAAGG AGAATGGGCC CGAGATGTTC TGCCAAATTC
      421 TCAGTAGCAT AATGTGAAAG AAGCCCTTAC ATTGTCCAGC CTCTGGCATC ATTAAAAACC
      481 GTAGCGGAAA CCAATTGTCT CTGTTCTTCC CTGGCACACC CTGGTAGCCC CATCCAGTTG
      541 TAGTACATCT CACACGCTGG CAACTTGGGA CAATCAGCAA CTTTTTTTTC TTTTAATTTT
      601 TTCAGCGCGA CATTTTGCCT CTTCTGCGAG AACAGACTTT TTCACCTCCA TCTCACCCCC
      661 CTTTGCACTT ATATAAATTG GACCAGTTCC TCCCATTGTA GAAAAAATTT TGCTGGACCT
      721 TTTTCTCTTT TTTTTGTCCT TTAGTTTCAT ACAATCTAAG TCTATCTACA TGTACAATGG
      781 GTAAGGAAAA GACTCACGTT TCGAGGCCGa gaTTAAATTC CAACATGGAT GCTGATTTAT
      841 ATGGGTATAA ATGGGCTaga GATAATGTCG GGCAATCAGG TGCGACAATC TATagaTTGT
      901 ATGGGAAGCC CGATGCGCCA GAGTTGTTTt TGAAACATGG CAAAGGTAGC GTTGCCAATG
      961 ATGTTACAGA TGAGATGGTC AGACTAAACT GGtTGACGGA ATTTATGCCT CTTCCGACCA
     1021 TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGCA
     1081 AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGt
     1141 TGGCAGTGTT CtTGagaaga TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG
     1201 ATagaGTATT TCGTCTCGCT CAGGCGCAAT CAagaATGAA TAACGGTTTG GTTGATGCGA
     1261 GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA
     1321 AGttgTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC
     1381 TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGAagaGTC GGAATCGCAG
     1441 ACagaTACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC
     1501 AGAAAagaCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC
     1561 ATTTGATGCT CGATGAGTTT TTCTAAGCGG CCGCtAACCA CTTGCAAAAT CCTTTGTATT
     1621 CTTGTTCTGC AAACATTTTG TCCAATCTCT TATCTTTCTA CGATGTTGAA GAACATACAT
     1681 TTTTGTTAGT CCAGCTTGTT ATCCTTTTTT TATTAAATAA AAAATTTTAT TGTTAAAGTT
     1741 GTTTTTCTTC TTTGTCTGTC CTGTTTTAAC TCATATATAT TCTATATATT ACAGAAAGAA
     1801 AGTAGATCAT CTAAAATAAA CATTTATTTG ATGAGACAAA GTAGGTTTTT TAGAATACAA
     1861 TGTAGCATCC TCTAATATAC AATAGAGTTG TTTTATAAAA GCGATACATT TAATATTATG
     1921 ACAGGACTGG TGAAGTGTAT AGAATTGTGC TAATCTGGCA AACGAACCCA AGAAGACGTT
     1981 AAAGCAAATA GTGCATTTTG TATTACAATC AAGTTCGTCG ACCATGCATT AGATCTTGCA
     2041 TGCCTCTAGA ACCTAGGTGG CGCCACAGCT GTGGATCCTC GTTAGTATTT CCGTGAAGGC
     2101 AGCCATTGTG AAAATTGAAC ATCACCTGAG GTATTTTAGC CACCAGAAGC GGCGGTACGG
     2161 AAGAAAGTGT GTACAATGGT TGGTGGTGGA ATTGCGTGCA TGCCTGATGG GGCAATATTA
     2221 ATTAGATAGA GCTTTGGTGA TATTAGTGGA TAATAGAATT CACAGAGAAG ACATCAGGAG
     2281 CAATTTCCAA GAGCCATTGA TGATGTAATT GCCCCAACAG CAAGATTCAG ATCTGACAAT
     2341 TGACCACCGT TTTGTAGAAG CAAAAAATCG TAGATTATCA CCAAGAGGGT TTTTCACCGA
     2401 ACCAGCAAAT AGAAACTATT CCGTAGAACT CGCCCAGGCT TTTTTGCTAG CACTTTCCAG
     2461 CAGTAGAACC GTCCAATTAA GTCAACAGGA ACCATTGAGG TCGAGCCCAA CCACCTGAAC
     2521 CCCCTCACGG TCGTGTCCCT ATTATTGATC CAGAGGGTGC CAGTTTCGGT AGCCAATATT
     2581 GGTTCATGGG TTTCTATGGC CCGGAGTGAG TTTGCAGGTT GGCCCCGGCG CCGTCTGCAG
     2641 GATGGGAGTT ATAGCGGCCA AACTTCACAT TTCGAAATCC TGCTGCAGCC AATCTGAAGA
     2701 ATTAATATAA ATTCGTGTCG AATCGCCGTC TGTGAAATTT CAGTACTTGA TTTTCTTTTC
     2761 TTCTTCTTTT TCTCTTTTGT TTCTTCAGAA TCAATTCACA TTTTTTCTTC CCTATAAACA
     2821 ATTCATCGGT ACCATGGTGA AGCGTGAGAA AAATGTCATC TATGGCCCTG AGCCTCTCCA
     2881 TCCTTTGGAG GATTTGACTG CCGGCGAAAT GTTGTTTCGT GCTCTCCGCA AGCACTCTCA
     2941 TTTGCCTCAA GCCTTGGTCG ATGTGGTCGG CGATGAATCT TTGAGCTACA AGGAGTTTTT
     3001 TGAGGCAACC GTCTTGTTGG CTCAGTCCCT CCACAATTGT GGCTACAAGA TGAACGACGT
     3061 CGTTAGTATC TGTGCTGAAA ACAATACCCG TTTCTTCATT CCAGTCATCG CCGCATGGTA
     3121 TATCGGTATG ATCGTGGCTC CAGTCAACGA GAGCTACATT CCCGACGAAT TGTGTAAAGT
     3181 CATGGGTATC TCTAAGCCAC AGATTGTCTT CACCACTAAG AATATTTTGA ACAAAGTCTT
     3241 GGAAGTCCAA AGCCGCACCA ACTTTATTAA GCGTATCATC ATCTTGGACA CTGTGGAGAA
     3301 TATTCACGGT TGCGAATCTT TGCCTAATTT CATCTCTCGC TATTCAGACG GCAACATCGC
     3361 AAACTTTAAA CCACTCCACT TCGACCCTGT GGAACAAGTT GCAGCCATTT TGTGTAGCAG
     3421 CGGTACTACT GGACTCCCAA AGGGAGTCAT GCAGACCCAT CAAAACATTT GCGTGCGTTT
     3481 GATCCATGCT CTCGATCCAC GtGTGGGCAC TCAGTTGATT CCTGGTGTCA CCGTCTTGGT
     3541 CTACTTGCCT TTCTTCCATG CTTTCGGCTT TAGCATTACT TTGGGTTACT TTATGGTCGG
     3601 TCTCCGCGTG ATTATGTTCC GCCGTTTTGA TCAGGAGGCT TTCTTGAAAG CCATCCAAGA
     3661 TTATGAAGTC CGCAGTGTCA TCAACGTGCC TAGCGTGATC TTGTTTTTGT CTAAGAGCCC
     3721 ACTCGTGGAC AAGTACGACT TGTCTTCAtT GCGTGAATTG TGTTGCGGTG CCGCTCCAtT
     3781 GGCTAAGGAG GTCGCTGAAG TGGCCGCCAA ACGCTTGAAT CTTCCAGGtA TTCGTTGTGG
     3841 CTTCGGCCTC ACCGAATCTA CCAGCGCTAA CATTCACTCT CTCGGtGATG AGTTTAAGAG
     3901 CGGCTCTTTG GGCCGTGTCA CTCCACTCAT GGCTGCTAAG ATCGCTGATC GCGAAACTGG
     3961 TAAGGCTTTG GGtCCGAACC AAGTGGGCGA GtTGTGTATC AAAGGCCCTA TGGTGAGCAA
     4021 GGGTTATGTC AATAACGTTG AAGCTACCAA GGAGGCCATC GACGACGACG GCTGGTTGCA
     4081 TTCTGGTGAT TTTGGATATT ACGACGAAGA TGAGCATTTT TACGTCGTGG ATCGTTACAA
     4141 GGAGTTGATC AAATACAAGG GTAGCCAGGT TGCTCCAGCT GAGTTGGAGG AGATTTTGTT
     4201 GAAAAATCCA TGCATTCGCG ATGTCGCTGT GGTCGGCATT CCTGATTTGG AGGCCGGCGA
     4261 ATTGCCTTCT GCTTTCGTTG TCAAGCAGCC TGGTAAAGAA ATTACCGCCA AAGAAGTGTA
     4321 TGATTACTTG GCTGAACGTG TGAGCCATAC TAAGTACTTG CGTGGCGGCG TGCGTTTTGT
     4381 TGACTCCATC CCTCGTAACG TAACAGGCAA AATTACCCGC AAGGAGTTGT TGAAACAATT
     4441 GTTGGAGAAG GCCGGCGGTT AGCTTAAGCA TTTTAGACAA GTGCCTATTT ATTCAGATTG
     4501 ATTTTTTATA TTGAATATGG ATGTTGATTA CGACTTGTGT TTGAAAAAGT GTTTACGAAT
     4561 AAAAGAACTT TTTTTGCAAC CGTTGCTAGT TGAAGTGTAG ACTCTGAATG CGAAGGGTTG
     4621 CAAATGTGCC CAGTAGAAAA AATATTCCAT ATTTTTCTGA ACTTAATTCA ATGAAATACG
     4681 AGTATTACTA CAACTGCCAA TATCGGAACA ATTTAACGAG AGCAAGCTAT ATAAAATGAA
     4741 TGTTCATTTA CACAAGCTAG AACCAAAACT TGAATTAAAA TTTAACATAA CTAAATGTAA
     4801 CTAGATAAAA GAGAGACATA ACCAAACGTT CAGAAGGCAG AAGAATTCAG TTAACTCCTG
     4861 CAGGAATCGA TTGGCCGGCC TCACGTGGAC GCGTTATTAA TCGTTTAAAC CTACGTAACC
     4921 GCGGCTTAAT TAAAGCTAGC CAGCGCTTGA GCTCTCTGCG GTGTCTACAA GGTCAGAAGT
     4981 ATTTTAACTC ATCCAAAAGC CCCAGACACC GCATTATTTC CCGGTGCATG CATTCTTCTC
     5041 TGACAAGTAC CTTCTCTCTC CATTGGCTGG ATAAAAGAAT CTGTAGGTCG CCGACATCTT
     5101 GTTTGTGTCT ACATGGCCAA CATACTTCTC TTACAATGTA TGTGCATATG CATATGTAAG
     5161 TGGAGCGTGG TGGGAGGCTT TATTGTAGAG CTATTGTTCT GACGGAGTAG GTGCAAACGG
     5221 AGTCTGTTTA GGTACAGTCA CCATCTCTGC GTATCTGTAC ATTTTCACTG GAGCTAGAAG
     5281 GAGCTACTCT TCGACTTCGA GAACAGGGGA GCAACCCAGT TTGTGTTCAA GGCGATTGTT
     5341 AGTTGCTCCG GCAAGATAAG GTTACATTAT TCTCTACGCT TTAGCGTTTA GGGGTACTCG
     5401 GATAAATGCC AGATGAAAAA CGCTAGGTGC ATACAGTACA AGTCGAGCCG ACATTGTTGG
     5461 ACCGCCCCGA TAATTATTGT ATGTTGTGTT TGCTTGTAAG ACCATGATTG ATATTTATTG
     5521 GGTGGTTCAG CAATCGGACT CGTTGATTAT TATTATTATT GACCCTCATG ATATACAATG
     5581 GATTTTATTT CTGAGCAATC GTAGCAAAAT ATTCGACGCA ATGTAGCTTG AGTTTATGAC
     5641 CTTAGCGGAC ATCACCAGCG CAGCTTTACA TTTTCCATTC TTTGGATTTA TTTTTCTTAT
     5701 CCACTAGGAG ATTAGAAGTC TAAGTATCAT GTAAGAAAGG AGATAATTGT TGTCTGCTGG
     5761 ATAATAAAAT TTTGCTGAGT TAGAAATGAG ATACGAATCA GCAAGATGTC TTTGGTTGCA
     5821 GCCTGTAGAG ATTTAGATTT CGACAATCTG ACTAAAATGT ACGAAGAGAA AAATAGACAC
     5881 ATTTAGTCAA TACTTCAGCG TTAAGGGTGC TATAGACTGT GCAACTTCGT TTCACTGCTG
     5941 ACATGAAATT CCAGAGACTT AACATATTAC ATGATTATAA ATAATTTGCG TATAATATGT
     6001 TTGGCATAAA GCGATTTCTG GAAAAGTTGC GAAAAATTCC GAATTACTAC AACTTACCAA
     6061 AAAAACAGAG GCATATTTTG CTAAATCACC ATATCCTATA CTGAGCTCCA TCGGAAAGAA
     6121 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     6181 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     6241 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     6301 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     6361 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     6421 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     6481 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     6541 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     6601 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     6661 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     6721 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     6781 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     6841 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     6901 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     6961 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     7021 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     7081 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     7141 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     7201 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     7261 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     7321 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     7381 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     7441 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     7501 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     7561 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     7621 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     7681 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     7741 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     7801 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     7861 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     7921 AGTGCCACCT GACGTC    
//
