LOCUS       WR24_pAllet_w_Pt        7702 bp ds-DNA     circular     22-MAY-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     ARS confirmed orientation
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(6709..7566)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2600..4228
                     /locus_tag="PsCO CBG99"
                     /label="PsCO CBG99"
                     /ApEinfo_label="PsCO CBG99"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5891..6558
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1383..1758
                     /locus_tag="Act term"
                     /label="Act term"
                     /ApEinfo_label="Act term"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        45..156
                     /locus_tag="Ps TEF1 promoter"
                     /label="Ps TEF1 promoter"
                     /ApEinfo_label="Ps TEF1 promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1839..2593
                     /locus_tag="Ptdh3"
                     /label="Ptdh3"
                     /ApEinfo_label="Ptdh3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4234..4608
                     /locus_tag="Adh2term"
                     /label="Adh2term"
                     /ApEinfo_label="Adh2term"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        157..770
                     /locus_tag="Ps TEF1 promoter(1)"
                     /label="Ps TEF1 promoter(1)"
                     /ApEinfo_label="Ps TEF1 promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             777..1352
                     /locus_tag="PsCOnat"
                     /label="PsCOnat"
                     /ApEinfo_label="PsCOnat"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4721..5868)
                     /locus_tag="Ps ARS from Chsome1"
                     /label="Ps ARS from Chsome1"
                     /ApEinfo_label="Ps ARS from Chsome1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5846..5868)
                     /locus_tag="PsARS5(SacI)"
                     /label="PsARS5(SacI)"
                     /ApEinfo_label="PsARS5(SacI)"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4721..4740
                     /locus_tag="PsARS3(SacI)"
                     /label="PsARS3(SacI)"
                     /ApEinfo_label="PsARS3(SacI)"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CCAATGATAC CGGTGCGATC GTGGGCCCTA GTACTTCCCT CGAGCCGTAC ACTTATTGTT
       61 AACTATGAAA CATATACTTA TGGACAAGGT TCACTGATTT GGACTGAAAA GCAAAAACGA
      121 GAAATGAGTC AACGTCTGAG ATCAATCCCT AGACCACCCG GGAAGGTCAT GTGTTTCATG
      181 AAGTACGATA AGGTTGGTAA CCGATTGACT CATTGGTTCG TGGCGGAGAA GTACGCAGAG
      241 TAAAACCGGG GCCGATTCGT GGTAAATTCT GGAATGATCC AGAGGCGCGA CATTTATGCA
      301 GACAATTTGT GTTTTGTCGC AAACGATGTT ATAGCGAAAT TTTTCACTCT GTCAGATAAA
      361 TGGATTTTGT CAAAAGGGGG AAGTAGAAGG AGAATGGGCC CGAGATGTTC TGCCAAATTC
      421 TCAGTAGCAT AATGTGAAAG AAGCCCTTAC ATTGTCCAGC CTCTGGCATC ATTAAAAACC
      481 GTAGCGGAAA CCAATTGTCT CTGTTCTTCC CTGGCACACC CTGGTAGCCC CATCCAGTTG
      541 TAGTACATCT CACACGCTGG CAACTTGGGA CAATCAGCAA CTTTTTTTTC TTTTAATTTT
      601 TTCAGCGCGA CATTTTGCCT CTTCTGCGAG AACAGACTTT TTCACCTCCA TCTCACCCCC
      661 CTTTGCACTT ATATAAATTG GACCAGTTCC TCCCATTGTA GAAAAAATTT TGCTGGACCT
      721 TTTTCTCTTT TTTTTGTCCT TTAGTTTCAT ACAATCTAAG TCTATCTACA TGTACAATGG
      781 GTACtACTCT TGACGACACG GCTTACCGtT ACagaACCAG TGTCCCaGGG GACGCCGAGG
      841 CCATCGAGGC AttgGATGGG TCCTTCACCA CCGACACCGT CTTCagaGTC ACCGCCACCG
      901 GGGACGGCTT CACCtTGaga GAGGTGCCGG TGGACCCGCC CttgACCAAG GTGTTCCCCG
      961 ACGACGAATC GGACGACGAA TCGGACGACG GGGAGGACGG CGACCCGGAC TCCagaACGT
     1021 TCGTCGCGTA CGGGGACGAC GGCGACttgG CGGGCTTCGT GGTCATCTCG TACTCGGCGT
     1081 GGAACagaag aCTtACCGTC GAGGACATCG AGGTCGCCCC GGAGCACaga GGGCACGGGG
     1141 TCGGGagaGC GTTGATGGGG CTCGCGACGG AGTTCGCCGG CGAGagaGGC GCCGGGCACC
     1201 TCTGGttgGA GGTCACCAAC GTCAACGCAC CGGCGATCCA CGCtTACaga agaATGGGGT
     1261 TCACCCTCTG CGGCttgGAC ACCGCCCTtT ACGACGGCAC CGCCTCGGAC GGCGAGagaC
     1321 AGGCGCTCTA CATGAGCATG CCCTGCCCCT AAGCGGCCGC tAACCACTTG CAAAATCCTT
     1381 TGTATTCTTG TTCTGCAAAC ATTTTGTCCA ATCTCTTATC TTTCTACGAT GTTGAAGAAC
     1441 ATACATTTTT GTTAGTCCAG CTTGTTATCC TTTTTTTATT AAATAAAAAA TTTTATTGTT
     1501 AAAGTTGTTT TTCTTCTTTG TCTGTCCTGT TTTAACTCAT ATATATTCTA TATATTACAG
     1561 AAAGAAAGTA GATCATCTAA AATAAACATT TATTTGATGA GACAAAGTAG GTTTTTTAGA
     1621 ATACAATGTA GCATCCTCTA ATATACAATA GAGTTGTTTT ATAAAAGCGA TACATTTAAT
     1681 ATTATGACAG GACTGGTGAA GTGTATAGAA TTGTGCTAAT CTGGCAAACG AACCCAAGAA
     1741 GACGTTAAAG CAAATAGTGC ATTTTGTATT ACAATCAAGT TCGTCGACCA TGCATTAGAT
     1801 CTTGCATGCC TCTAGAACCT AGGTGGCGCC ACAGCTGTGG ATCCTCGTTA GTATTTCCGT
     1861 GAAGGCAGCC ATTGTGAAAA TTGAACATCA CCTGAGGTAT TTTAGCCACC AGAAGCGGCG
     1921 GTACGGAAGA AAGTGTGTAC AATGGTTGGT GGTGGAATTG CGTGCATGCC TGATGGGGCA
     1981 ATATTAATTA GATAGAGCTT TGGTGATATT AGTGGATAAT AGAATTCACA GAGAAGACAT
     2041 CAGGAGCAAT TTCCAAGAGC CATTGATGAT GTAATTGCCC CAACAGCAAG ATTCAGATCT
     2101 GACAATTGAC CACCGTTTTG TAGAAGCAAA AAATCGTAGA TTATCACCAA GAGGGTTTTT
     2161 CACCGAACCA GCAAATAGAA ACTATTCCGT AGAACTCGCC CAGGCTTTTT TGCTAGCACT
     2221 TTCCAGCAGT AGAACCGTCC AATTAAGTCA ACAGGAACCA TTGAGGTCGA GCCCAACCAC
     2281 CTGAACCCCC TCACGGTCGT GTCCCTATTA TTGATCCAGA GGGTGCCAGT TTCGGTAGCC
     2341 AATATTGGTT CATGGGTTTC TATGGCCCGG AGTGAGTTTG CAGGTTGGCC CCGGCGCCGT
     2401 CTGCAGGATG GGAGTTATAG CGGCCAAACT TCACATTTCG AAATCCTGCT GCAGCCAATC
     2461 TGAAGAATTA ATATAAATTC GTGTCGAATC GCCGTCTGTG AAATTTCAGT ACTTGATTTT
     2521 CTTTTCTTCT TCTTTTTCTC TTTTGTTTCT TCAGAATCAA TTCACATTTT TTCTTCCCTA
     2581 TAAACAATTC ATCGGTACCA TGGTGAAGCG TGAGAAAAAT GTCATCTATG GCCCTGAGCC
     2641 TCTCCATCCT TTGGAGGATT TGACTGCCGG CGAAATGTTG TTTCGTGCTC TCCGCAAGCA
     2701 CTCTCATTTG CCTCAAGCCT TGGTCGATGT GGTCGGCGAT GAATCTTTGA GCTACAAGGA
     2761 GTTTTTTGAG GCAACCGTCT TGTTGGCTCA GTCCCTCCAC AATTGTGGCT ACAAGATGAA
     2821 CGACGTCGTT AGTATCTGTG CTGAAAACAA TACCCGTTTC TTCATTCCAG TCATCGCCGC
     2881 ATGGTATATC GGTATGATCG TGGCTCCAGT CAACGAGAGC TACATTCCCG ACGAATTGTG
     2941 TAAAGTCATG GGTATCTCTA AGCCACAGAT TGTCTTCACC ACTAAGAATA TTTTGAACAA
     3001 AGTCTTGGAA GTCCAAAGCC GCACCAACTT TATTAAGCGT ATCATCATCT TGGACACTGT
     3061 GGAGAATATT CACGGTTGCG AATCTTTGCC TAATTTCATC TCTCGCTATT CAGACGGCAA
     3121 CATCGCAAAC TTTAAACCAC TCCACTTCGA CCCTGTGGAA CAAGTTGCAG CCATTTTGTG
     3181 TAGCAGCGGT ACTACTGGAC TCCCAAAGGG AGTCATGCAG ACCCATCAAA ACATTTGCGT
     3241 GCGTTTGATC CATGCTCTCG ATCCACGtGT GGGCACTCAG TTGATTCCTG GTGTCACCGT
     3301 CTTGGTCTAC TTGCCTTTCT TCCATGCTTT CGGCTTTAGC ATTACTTTGG GTTACTTTAT
     3361 GGTCGGTCTC CGCGTGATTA TGTTCCGCCG TTTTGATCAG GAGGCTTTCT TGAAAGCCAT
     3421 CCAAGATTAT GAAGTCCGCA GTGTCATCAA CGTGCCTAGC GTGATCTTGT TTTTGTCTAA
     3481 GAGCCCACTC GTGGACAAGT ACGACTTGTC TTCAtTGCGT GAATTGTGTT GCGGTGCCGC
     3541 TCCAtTGGCT AAGGAGGTCG CTGAAGTGGC CGCCAAACGC TTGAATCTTC CAGGtATTCG
     3601 TTGTGGCTTC GGCCTCACCG AATCTACCAG CGCTAACATT CACTCTCTCG GtGATGAGTT
     3661 TAAGAGCGGC TCTTTGGGCC GTGTCACTCC ACTCATGGCT GCTAAGATCG CTGATCGCGA
     3721 AACTGGTAAG GCTTTGGGtC CGAACCAAGT GGGCGAGtTG TGTATCAAAG GCCCTATGGT
     3781 GAGCAAGGGT TATGTCAATA ACGTTGAAGC TACCAAGGAG GCCATCGACG ACGACGGCTG
     3841 GTTGCATTCT GGTGATTTTG GATATTACGA CGAAGATGAG CATTTTTACG TCGTGGATCG
     3901 TTACAAGGAG TTGATCAAAT ACAAGGGTAG CCAGGTTGCT CCAGCTGAGT TGGAGGAGAT
     3961 TTTGTTGAAA AATCCATGCA TTCGCGATGT CGCTGTGGTC GGCATTCCTG ATTTGGAGGC
     4021 CGGCGAATTG CCTTCTGCTT TCGTTGTCAA GCAGCCTGGT AAAGAAATTA CCGCCAAAGA
     4081 AGTGTATGAT TACTTGGCTG AACGTGTGAG CCATACTAAG TACTTGCGTG GCGGCGTGCG
     4141 TTTTGTTGAC TCCATCCCTC GTAACGTAAC AGGCAAAATT ACCCGCAAGG AGTTGTTGAA
     4201 ACAATTGTTG GAGAAGGCCG GCGGTTAGCT TAAGCATTTT AGACAAGTGC CTATTTATTC
     4261 AGATTGATTT TTTATATTGA ATATGGATGT TGATTACGAC TTGTGTTTGA AAAAGTGTTT
     4321 ACGAATAAAA GAACTTTTTT TGCAACCGTT GCTAGTTGAA GTGTAGACTC TGAATGCGAA
     4381 GGGTTGCAAA TGTGCCCAGT AGAAAAAATA TTCCATATTT TTCTGAACTT AATTCAATGA
     4441 AATACGAGTA TTACTACAAC TGCCAATATC GGAACAATTT AACGAGAGCA AGCTATATAA
     4501 AATGAATGTT CATTTACACA AGCTAGAACC AAAACTTGAA TTAAAATTTA ACATAACTAA
     4561 ATGTAACTAG ATAAAAGAGA GACATAACCA AACGTTCAGA AGGCAGAAGA ATTCAGTTAA
     4621 CTCCTGCAGG AATCGATTGG CCGGCCTCAC GTGGACGCGT TATTAATCGT TTAAACCTAC
     4681 GTAACCGCGG CTTAATTAAA GCTAGCCAGC GCTTGAGCTC TCTGCGGTGT CTACAAGGTC
     4741 AGAAGTATTT TAACTCATCC AAAAGCCCCA GACACCGCAT TATTTCCCGG TGCATGCATT
     4801 CTTCTCTGAC AAGTACCTTC TCTCTCCATT GGCTGGATAA AAGAATCTGT AGGTCGCCGA
     4861 CATCTTGTTT GTGTCTACAT GGCCAACATA CTTCTCTTAC AATGTATGTG CATATGCATA
     4921 TGTAAGTGGA GCGTGGTGGG AGGCTTTATT GTAGAGCTAT TGTTCTGACG GAGTAGGTGC
     4981 AAACGGAGTC TGTTTAGGTA CAGTCACCAT CTCTGCGTAT CTGTACATTT TCACTGGAGC
     5041 TAGAAGGAGC TACTCTTCGA CTTCGAGAAC AGGGGAGCAA CCCAGTTTGT GTTCAAGGCG
     5101 ATTGTTAGTT GCTCCGGCAA GATAAGGTTA CATTATTCTC TACGCTTTAG CGTTTAGGGG
     5161 TACTCGGATA AATGCCAGAT GAAAAACGCT AGGTGCATAC AGTACAAGTC GAGCCGACAT
     5221 TGTTGGACCG CCCCGATAAT TATTGTATGT TGTGTTTGCT TGTAAGACCA TGATTGATAT
     5281 TTATTGGGTG GTTCAGCAAT CGGACTCGTT GATTATTATT ATTATTGACC CTCATGATAT
     5341 ACAATGGATT TTATTTCTGA GCAATCGTAG CAAAATATTC GACGCAATGT AGCTTGAGTT
     5401 TATGACCTTA GCGGACATCA CCAGCGCAGC TTTACATTTT CCATTCTTTG GATTTATTTT
     5461 TCTTATCCAC TAGGAGATTA GAAGTCTAAG TATCATGTAA GAAAGGAGAT AATTGTTGTC
     5521 TGCTGGATAA TAAAATTTTG CTGAGTTAGA AATGAGATAC GAATCAGCAA GATGTCTTTG
     5581 GTTGCAGCCT GTAGAGATTT AGATTTCGAC AATCTGACTA AAATGTACGA AGAGAAAAAT
     5641 AGACACATTT AGTCAATACT TCAGCGTTAA GGGTGCTATA GACTGTGCAA CTTCGTTTCA
     5701 CTGCTGACAT GAAATTCCAG AGACTTAACA TATTACATGA TTATAAATAA TTTGCGTATA
     5761 ATATGTTTGG CATAAAGCGA TTTCTGGAAA AGTTGCGAAA AATTCCGAAT TACTACAACT
     5821 TACCAAAAAA ACAGAGGCAT ATTTTGCTAA ATCACCATAT CCTATACTGA GCTCCATCGG
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     6721 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     6781 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     6841 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     6901 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     6961 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     7021 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     7081 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     7141 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     7201 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     7261 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     7321 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     7381 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     7441 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     7501 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     7561 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     7621 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC
     7681 CCGAAAAGTG CCACCTGACG TC   
//
