LOCUS       WR76                    7237 bp ds-DNA     circular     22-MAY-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(6244..7101)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3053..3763
                     /locus_tag="coCherry"
                     /label="coCherry"
                     /ApEinfo_label="coCherry"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5426..6093
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1836..2211
                     /locus_tag="T-ACT1"
                     /label="T-ACT1"
                     /ApEinfo_label="T-ACT1"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             777..1805
                     /locus_tag="PsCOhph"
                     /label="PsCOhph"
                     /ApEinfo_label="PsCOhph"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4256..5403)
                     /locus_tag="Ps ARS from Chsome1"
                     /label="Ps ARS from Chsome1"
                     /ApEinfo_label="Ps ARS from Chsome1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        45..770
                     /locus_tag="P-TEF1"
                     /label="P-TEF1"
                     /ApEinfo_label="P-TEF1"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2298..3046
                     /locus_tag="P-TDH3"
                     /label="P-TDH3"
                     /ApEinfo_label="P-TDH3"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      3770..4143
                     /locus_tag="T-ADH2"
                     /label="T-ADH2"
                     /ApEinfo_label="T-ADH2"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2883..2901
                     /locus_tag="PtdhEndSEQ5"
                     /label="PtdhEndSEQ5"
                     /ApEinfo_label="PtdhEndSEQ5"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3893..3912)
                     /locus_tag="Tadh2SEQ3"
                     /label="Tadh2SEQ3"
                     /ApEinfo_label="Tadh2SEQ3"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CCAATGATAC CGGTGCGATC GTGGGCCCTA GTACTTCCCT CGAGCCGTAC ACTTATTGTT
       61 AACTATGAAA CATATACTTA TGGACAAGGT TCACTGATTT GGACTGAAAA GCAAAAACGA
      121 GAAATGAGTC AACGTCTGAG ATCAATCCCT AGACCACCCG GGAAGGTCAT GTGTTTCATG
      181 AAGTACGATA AGGTTGGTAA CCGATTGACT CATTGGTTCG TGGCGGAGAA GTACGCAGAG
      241 TAAAACCGGG GCCGATTCGT GGTAAATTCT GGAATGATCC AGAGGCGCGA CATTTATGCA
      301 GACAATTTGT GTTTTGTCGC AAACGATGTT ATAGCGAAAT TTTTCACTCT GTCAGATAAA
      361 TGGATTTTGT CAAAAGGGGG AAGTAGAAGG AGAATGGGCC CGAGATGTTC TGCCAAATTC
      421 TCAGTAGCAT AATGTGAAAG AAGCCCTTAC ATTGTCCAGC CTCTGGCATC ATTAAAAACC
      481 GTAGCGGAAA CCAATTGTCT CTGTTCTTCC CTGGCACACC CTGGTAGCCC CATCCAGTTG
      541 TAGTACATCT CACACGCTGG CAACTTGGGA CAATCAGCAA CTTTTTTTTC TTTTAATTTT
      601 TTCAGCGCGA CATTTTGCCT CTTCTGCGAG AACAGACTTT TTCACCTCCA TCTCACCCCC
      661 CTTTGCACTT ATATAAATTG GACCAGTTCC TCCCATTGTA GAAAAAATTT TGCTGGACCT
      721 TTTTCTCTTT TTTTTGTCCT TTAGTTTCAT ACAATCTAAG TCTATCTACA TGTACAATGG
      781 GTAAAAAGCC TGAACTCACC GCGACcTCTG TCGAGAAGTT TTTGATCGAA AAGTTCGACA
      841 GCGTCTCCGA CTTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG
      901 TAGGAGGGCG TGGATATGTC TTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC
      961 GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG
     1021 GGGAGTTCAG CGAGAGCTTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC
     1081 AAGACTTGCC TGAAACCGAA TTGCCCGCTG TTTTGCAGCC GGTCGCGGAG GCCATGGATG
     1141 CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA
     1201 TCGGTCAATA CACTACATGG CGTGATTTCA TTTGCGCGAT TGCTGATCCC CATGTGTATC
     1261 ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGT
     1321 TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT
     1381 CCAACAATGT CTTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA
     1441 TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
     1501 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCAC
     1561 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
     1621 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
     1681 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
     1741 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
     1801 AATAAGCGGC CGCtAACCAC TTGCAAAATC CTTTGTATTC TTGTTCTGCA AACATTTTGT
     1861 CCAATCTCTT ATCTTTCTAC GATGTTGAAG AACATACATT TTTGTTAGTC CAGCTTGTTA
     1921 TCCTTTTTTT ATTAAATAAA AAATTTTATT GTTAAAGTTG TTTTTCTTCT TTGTCTGTCC
     1981 TGTTTTAACT CATATATATT CTATATATTA CAGAAAGAAA GTAGATCATC TAAAATAAAC
     2041 ATTTATTTGA TGAGACAAAG TAGGTTTTTT AGAATACAAT GTAGCATCCT CTAATATACA
     2101 ATAGAGTTGT TTTATAAAAG CGATACATTT AATATTATGA CAGGACTGGT GAAGTGTATA
     2161 GAATTGTGCT AATCTGGCAA ACGAACCCAA GAAGACGTTA AAGCAAATAG TGCATTTTGT
     2221 ATTACAATCA AGTTCGTCGA CCATGCATTA GATCTTGCAT GCCTCTAGAA CCTAGGTGGC
     2281 GCCACAGCTG TGGATCCTCG TTAGTATTTC CGTGAAGGCA GCCATTGTGA AAATTGAACA
     2341 TCACCTGAGG TATTTTAGCC ACCAGAAGCG GCGGTACGGA AGAAAGTGTG TACAATGGTT
     2401 GGTGGTGGAA TTGCGTGCAT GCCTGATGGG GCAATATTAA TTAGATAGAG CTTTGGTGAT
     2461 ATTAGTGGAT AATAGAATTC ACAGAGAAGA CATCAGGAGC AATTTCCAAG AGCCATTGAT
     2521 GATGTAATTG CCCCAACAGC AAGATTCAGA TCTGACAATT GACCACCGTT TTGTAGAAGC
     2581 AAAAAATCGT AGATTATCAC CAAGAGGGTT TTTCACCGAA CCAGCAAATA GAAACTATTC
     2641 CGTAGAACTC GCCCAGGCTT TTTTGCTAGC ACTTTCCAGC AGTAGAACCG TCCAATTAAG
     2701 TCAACAGGAA CCATTGAGGT CGAGCCCAAC CACCTGAACC CCCTCACGGT CGTGTCCCTA
     2761 TTATTGATCC AGAGGGTGCC AGTTTCGGTA GCCAATATTG GTTCATGGGT TTCTATGGCC
     2821 CGGAGTGAGT TTGCAGGTTG GCCCCGGCGC CGTCTGCAGG ATGGGAGTTA TAGCGGCCAA
     2881 ACTTCACATT TCGAAATCCT GCTGCAGCCA ATCTGAAGAA TTAATATAAA TTCGTGTCGA
     2941 ATCGCCGTCT GTGAAATTTC AGTACTTGAT TTTCTTTTCT TCTTCTTTTT CTCTTTTGTT
     3001 TCTTCAGAAT CAATTCACAT TTTTTCTTCC CTATAAACAA TTCATCGGTA CCATGGTTTC
     3061 AAAAGGTGAA GAAGATAATA TGGCTATTAT TAAAGAATTT ATGAGATTTA AAGTTCATAT
     3121 GGAAGGTTCA GTTAATGGTC ATGAATTTGA AATTGAAGGT GAAGGTGAAG GTAGACCATA
     3181 TGAAGGTACT CAAACTGCTA AATTGAAAGT TACTAAAGGT GGTCCATTAC CATTTGCTTG
     3241 GGATATTTTG TCACCACAAT TTATGTATGG TTCAAAAGCT TATGTTAAAC ATCCAGCTGA
     3301 TATTCCAGAT TATTTAAAAT TGTCATTTCC AGAAGGTTTT AAATGGGAAA GAGTTATGAA
     3361 TTTTGAAGAT GGTGGTGTTG TTACTGTTAC TCAAGATTCA TCATTACAAG ATGGTGAATT
     3421 TATTTATAAA GTTAAATTGA GAGGTACTAA TTTTCCATCA GATGGTCCAG TTATGCAAAA
     3481 AAAAACTATG GGTTGGGAAG CTTCATCAGA AAGAATGTAT CCAGAAGATG GTGCTTTAAA
     3541 AGGTGAAATT AAACAAAGAT TGAAATTAAA AGATGGTGGT CATTATGATG CTGAAGTTAA
     3601 AACTACTTAT AAAGCTAAAA AACCAGTTCA ATTACCAGGT GCTTATAATG TTAATATTAA
     3661 ATTGGATATT ACTTCACATA ATGAAGATTA TACTATTGTT GAACAATATG AAAGAGCTGA
     3721 AGGTAGACAT TCAACTGGTG GTATGGATGA ATTATATAAA TAACTTAAGC ATTTTAGACA
     3781 AGTGCCTATT TATTCAGATT GATTTTTTAT ATTGAATATG GATGTTGATT ACGACTTGTG
     3841 TTTGAAAAAG TGTTTACGAA TAAAAGAACT TTTTTTGCAA CCGTTGCTAG TTGAAGTGTA
     3901 GACTCTGAAT GCGAAGGGTT GCAAATGTGC CCAGTAGAAA AAATATTCCA TATTTTTCTG
     3961 AACTTAATTC AATGAAATAC GAGTATTACT ACAACTGCCA ATATCGGAAC AATTTAACGA
     4021 GAGCAAGCTA TATAAAATGA ATGTTCATTT ACACAAGCTA GAACCAAAAC TTGAATTAAA
     4081 ATTTAACATA ACTAAATGTA ACTAGATAAA AGAGAGACAT AACCAAACGT TCAGAAGGCA
     4141 GAAGAATTCA GTTAACTCCT GCAGGAATCG ATTGGCCGGC CTCACGTGGA CGCGTTATTA
     4201 ATCGTTTAAA CCTACGTAAC CGCGGCTTAA TTAAAGCTAG CCAGCGCTTG AGCTCTCTGC
     4261 GGTGTCTACA AGGTCAGAAG TATTTTAACT CATCCAAAAG CCCCAGACAC CGCATTATTT
     4321 CCCGGTGCAT GCATTCTTCT CTGACAAGTA CCTTCTCTCT CCATTGGCTG GATAAAAGAA
     4381 TCTGTAGGTC GCCGACATCT TGTTTGTGTC TACATGGCCA ACATACTTCT CTTACAATGT
     4441 ATGTGCATAT GCATATGTAA GTGGAGCGTG GTGGGAGGCT TTATTGTAGA GCTATTGTTC
     4501 TGACGGAGTA GGTGCAAACG GAGTCTGTTT AGGTACAGTC ACCATCTCTG CGTATCTGTA
     4561 CATTTTCACT GGAGCTAGAA GGAGCTACTC TTCGACTTCG AGAACAGGGG AGCAACCCAG
     4621 TTTGTGTTCA AGGCGATTGT TAGTTGCTCC GGCAAGATAA GGTTACATTA TTCTCTACGC
     4681 TTTAGCGTTT AGGGGTACTC GGATAAATGC CAGATGAAAA ACGCTAGGTG CATACAGTAC
     4741 AAGTCGAGCC GACATTGTTG GACCGCCCCG ATAATTATTG TATGTTGTGT TTGCTTGTAA
     4801 GACCATGATT GATATTTATT GGGTGGTTCA GCAATCGGAC TCGTTGATTA TTATTATTAT
     4861 TGACCCTCAT GATATACAAT GGATTTTATT TCTGAGCAAT CGTAGCAAAA TATTCGACGC
     4921 AATGTAGCTT GAGTTTATGA CCTTAGCGGA CATCACCAGC GCAGCTTTAC ATTTTCCATT
     4981 CTTTGGATTT ATTTTTCTTA TCCACTAGGA GATTAGAAGT CTAAGTATCA TGTAAGAAAG
     5041 GAGATAATTG TTGTCTGCTG GATAATAAAA TTTTGCTGAG TTAGAAATGA GATACGAATC
     5101 AGCAAGATGT CTTTGGTTGC AGCCTGTAGA GATTTAGATT TCGACAATCT GACTAAAATG
     5161 TACGAAGAGA AAAATAGACA CATTTAGTCA ATACTTCAGC GTTAAGGGTG CTATAGACTG
     5221 TGCAACTTCG TTTCACTGCT GACATGAAAT TCCAGAGACT TAACATATTA CATGATTATA
     5281 AATAATTTGC GTATAATATG TTTGGCATAA AGCGATTTCT GGAAAAGTTG CGAAAAATTC
     5341 CGAATTACTA CAACTTACCA AAAAAACAGA GGCATATTTT GCTAAATCAC CATATCCTAT
     5401 ACTGAGCTCC ATCGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     6241 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     6301 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     6361 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     6421 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     6481 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     6541 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     6601 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     6661 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     6721 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     6781 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     6841 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     6901 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     6961 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     7021 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     7081 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     7141 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     7201 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC  
//
