LOCUS       mCherry-CDMPR        6721 bp DNA     circular SYN 22-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6721
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     CDS             complement(213..1073)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1074..1178)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1237..1289
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     enhancer        1444..1823
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1824..2027
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2023..2056
                     /label=5' LTR (truncated) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=18.8"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: RIGHT"
     promoter        2072..2090
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2163..2276
                     /codon_start=1
                     /label=STIM1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.5"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGA"
     CDS             2286..2762
                     /codon_start=1
                     /label=SAV_STRAV (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=86.9"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="DPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYES
                     AVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARI
                     NTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ"
     intron          2855..3084
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
                     /note="color: #------; direction: RIGHT"
     misc_feature    3267..3729
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: RIGHT"
     sig_peptide     3756..3814
                     /label=IL-2 signal sequence
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.3"
                     /note="/other=sig_peptide"
                     /note="color: #cc99b2; direction: RIGHT"
     CDS             3816..3926
                     /label=SBP
                     /note="color: #993366; direction: RIGHT"
     CDS             3936..4640
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             4653..5405
                     /codon_start=1
                     /label=Human M6PR without first 26 amino acids
                     /label=M6PR (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.6"
                     /note="/match_length=90.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="TEEKTCDLVGEKGRESEKELALVKRLKPLFNKSFESTVGQGSDTY
                     IYIFRVCREAGNHTSGAGLVQINKSNGKETVVGRLNETHIFNGSNWIMLIYKGGDEYDN
                     HCGKEQRRAVVMISCNRHTLADNFNPVSEERGKVQDCFYLFEMDSSLACSPEISHLSVG
                     SILLVTFASLVAVYVVGGFLYQRLVVGAKGMEQFPHLAFWQDLGNLVADGCDFVCRSKP
                     RNVPAAYRGVGDDQLGEESEERDDHLLPM"
     misc_feature    4692..4694
                     /label=AAA (K) to AGA (R) substitution
                     /label=AGA (R) to AAA (K) substitution
                     /note="color: #a6acb3"
     CDS             5443..5527
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="*S*SAIPHL*RFYLL*KTSHTSP*T*NI"
     polyA_signal    5550..5671
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    5682..5733
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5797..5813
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        5821..5851
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    5866..5887
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     CDS             complement(5900..5992)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(join(6175..6721,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     1261 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     1321 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     1381 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     1441 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1501 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1561 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1621 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1681 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1741 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1801 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     1861 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     1921 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     1981 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     2041 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     2101 cttggtaccg agctcggatc gatatctgcg gcctagctag cgcttaaggc ctgttaaccg
     2161 gtatggatgt atgcgtccgt cttgccctgt ggctcctctg gggactcctc ctgcaccagg
     2221 gccagagcct cagccatagt cacagtgaga aggcgacagg aaccagctcg ggcgccggtt
     2281 taaacgaccc tagcaaagac tcaaaagctc aggtgtccgc tgccgaggct ggcattactg
     2341 gaacatggta caatcagctc gggagcacct ttattgtgac tgctggagcc gatggagccc
     2401 tcaccggaac atacgaatct gctgtgggaa acgccgaatc acggtacgtc ctcactggcc
     2461 gatacgatag tgcccctgcc accgacggat ctgggactgc cctgggatgg actgtcgctt
     2521 ggaaaaacaa ctaccggaat gctcattctg ccacaacatg gagtggacag tacgtgggag
     2581 gcgctgaggc tagaatcaat acacagtggc tgctcacatc tggcacaacc gaggcaaatg
     2641 cttggaaatc caccctggtg ggacatgaca cattcaccaa agtgaaaccc tccgccgctt
     2701 caatcgatgc cgccaaaaaa gccggagtca acaacggcaa tcctctggat gccgtccagc
     2761 agctcggacc gaaagatgaa ctgtgagcgg ccgcatagat aactgatcca gtgtgctgga
     2821 attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc
     2881 atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
     2941 cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg
     3001 ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca
     3061 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg
     3121 catctagggc ggccaattcc gcccctctcc ccccccccct ctccctcccc cccccctaac
     3181 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3241 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3301 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3361 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3421 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3481 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3541 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3601 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3661 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3721 cgatgataag cttgccacaa cccgggaggc gcgccatgta caggatgcaa ctcctgtctt
     3781 gcattgcact aagtcttgca cttgtcacga attccgacga gaagaccact ggttggcgag
     3841 gtggacacgt tgttgaagga ctggctgggg aacttgaaca acttcgtgca cgactggagc
     3901 atcacccaca aggtcaacgt gaaccacctg caggtgtgag caagggcgag gaggataaca
     3961 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     4021 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     4081 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     4141 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     4201 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     4261 tgaccgtgac ccaggactcc tccctacagg acggcgagtt catctacaag gtgaagctgc
     4321 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4381 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     4441 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     4501 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     4561 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     4621 gcatggacga gctgtacaag ggccggccag acacagaaga aaaaacttgc gacttggtag
     4681 gagaaaaggg tagagagtca gagaaagagt tggctctagt gaagaggctg aaaccactgt
     4741 ttaataaaag ctttgagagc actgtgggcc agggttcaga cacatacatc tacatcttca
     4801 gggtgtgccg ggaagctggc aaccacactt ctggggcagg cctggtgcaa atcaacaaaa
     4861 gtaatgggaa ggagacagtg gtagggagac tcaacgagac tcacatcttc aacggaagta
     4921 attggatcat gctgatctat aaagggggtg atgaatatga caaccactgt ggcaaggagc
     4981 agcgtcgtgc agtggtgatg atctcctgca atcgacacac cctagcggac aattttaacc
     5041 ctgtgtctga ggagcgtggc aaagtccaag attgtttcta cctctttgag atggatagca
     5101 gcctggcctg ttcaccagag atctcccacc tcagtgtggg ttccatctta cttgtcacgt
     5161 ttgcatcact ggttgctgtt tatgttgttg gggggttcct ataccagcga ctggtagtgg
     5221 gagccaaagg aatggagcag tttccccact tagccttctg gcaggatctt ggcaacctgg
     5281 tagcagatgg ctgtgacttt gtctgccgtt ctaaacctcg aaatgtgcct gcagcatatc
     5341 gtggtgtggg ggatgaccag ctgggggagg agtcagaaga aagggatgac catttattac
     5401 caatgtaggg cctcgagggc caattaatta actctagata actgatcata atcagccata
     5461 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5521 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5581 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5641 gtggtttgtc caaactcatc aatgtatctt aacgcgtcga gtgcattcta gttgtggttt
     5701 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5761 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5821 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5881 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5941 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6001 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6061 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6601 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6721 t
//
