LOCUS       FLAG-GGGGS-RPS28        5362 bp    DNA     circular UNA 13-DEC-2022
DEFINITION  FLAG-GGGGS-RPS28 PCR Product inserted into pcDNA5/FRT/TO (modified)
            (bases 1032 to 1000).
ACCESSION   urn.local...6n-fhqcyzb
VERSION     urn.local...6n-fhqcyzb
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          order(1..>1000,<1257..5362)
                     /organism="pcDNA5/FRT/TO"
                     /mol_type="other DNA"
                     /label="source pcDNA5/FRT/TO"
     misc_feature    206..211
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI"
                     /note="Geneious type: restriction site"
     promoter        236..812
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     primer_bind     743..762
                     /Sequence="ATGTCGTAACAACTCCGCCC"
                     /Hairpin_Tm="39.3"
                     /Tm="61.1"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="p5_CMVseq_CJB"
                     /Transferred_Similarity="100.00%"
                     /label="p5_CMVseq_CJB"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     primer_bind     769..789
                     /created_by="primer3"
                     /annotation_group="5183167055486500564b6a49-190d-4a05-8a3f
                     -2628deb7e402"
                     /%GC="66.7"
                     /Tm="68.1"
                     /Hairpin_Tm="52.4"
                     /Self_Dimer_Tm="3.5"
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="CMV-Forward"
                     /Transferred_Similarity="100.00%"
                     /label="CMV-Forward"
     promoter        770..893
                     /label="CMV_promoter"
     promoter        782..955
                     /label="CMV2_promoter"
     misc_feature    820..859
                     /label="tetO"
     misc_feature    869..893
                     /label="LNCX_primer"
     primer_bind     928..947
                     /Sequence="ATAGAAGACACCGGGACCGA"
                     /Hairpin_Tm="42.8"
                     /Tm="61.0"
                     /Self_Dimer_Tm="13.1"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="p5_ins_seqF"
     misc_feature    968..975
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI"
                     /note="Geneious type: restriction site"
     misc_feature    975..980
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     misc_feature    978..983
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    984..989
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    984..989
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Asp718I"
                     /note="Geneious type: restriction site"
     ligation        997..1000
                     /label="Ligation"
     misc_feature    1001..1256
                     /Source="referenceToDocument: urn:local:.:66-fhqcy3a"
                     /label="FLAG-GGGGS-RPS28 PCR Product"
                     /note="Geneious type: Inserted sequence"
     primer_bind     1002..1065
                     /Sequence="ATGGATTACAAGGACGACGATGACAAGGGUGGCGGUGGCTCTATGGA
                     CACCAGCCGTGTGCAGC"
                     /Extension="ACTGGGATCC"
                     /modified_by="User"
                     /label="RPS_pcD_F"
     misc_feature    1002..1004
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Affinity_tag    1005..1028
                     /created_by="User"
                     /label="FLAG"
     misc_feature    1029..1253
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             1044..1253
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="RPS28"
     misc_feature    complement(1233..1253)
                     /Sequence="CTAGCGCAACCTCCGGGCTTC"
                     /Hairpin_Tm="49.3"
                     /Tm="67.0"
                     /Self_Dimer_Tm="7.0"
                     /%GC="66.7"
                     /created_by="primer3"
                     /Extension="ACTGGATATC"
                     /label="RPS_pcD_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1259..1270
                     /Recognition_pattern="CCANNNNN^NTGG"
                     /annotation_group="CCANNNNNNTGG"
                     /label="BstXI"
                     /note="Geneious type: restriction site"
     misc_feature    1270..1277
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    1277..1282
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    1277..1282
                     /Recognition_pattern="C^YCGRG"
                     /annotation_group="CYCGRG"
                     /label="AvaI"
                     /note="Geneious type: restriction site"
     misc_feature    1288..1294
                     /Recognition_pattern="RG^GNCCY"
                     /annotation_group="RGGNCCY"
                     /label="EcoO109I"
                     /note="Geneious type: restriction site"
     misc_feature    1289..1294
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    1295..1302
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1314..1331)
                     /label="BGH_rev_primer"
     primer_bind     complement(1314..1331)
                     /created_by="primer3"
                     /annotation_group="5183155530197600bf0dbc35-0a3f-4d3a-aa85
                     -9555e56d40c8"
                     /%GC="55.6"
                     /Tm="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="BGHR"
                     /Transferred_Similarity="100.00%"
                     /label="BGHR"
     terminator      1317..1544
                     /label="bGH_PA_terminator"
     misc_feature    complement(1336..1355)
                     /Sequence="GGCAAACAACAGATGGCTGG"
                     /Hairpin_Tm="46.6"
                     /Tm="60.8"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="p5_ins_seqR"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1516..1521
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    1828..1875
                     /label="FRT"
     CDS             complement(1856..2638)
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFGQGSSYTF*"
                     /label="ORF frame 1"
     misc_feature    1878..1883
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     gene            1895..2902
                     /gene="hygroB"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFGQGSSYTF*"
                     /label="hygroB"
     CDS             1955..2905
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="ORF frame 2"
     terminator      3038..3157
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="SV40_PA_terminator"
     primer_bind     complement(3072..3091)
                     /created_by="primer3"
                     /annotation_group="58594628542739001efe2885-6483-4256-ba6a
                     -adf941c615db"
                     /%GC="35.0"
                     /Tm="51.1"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAAATTTGTGATGCTATTGC"
                     /Transferred_From="SV40pA-R"
                     /Transferred_Similarity="100.00%"
                     /label="SV40pA-R"
     misc_feature    3126..3145
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="EBV_rev_primer"
     promoter        complement(3201..3219)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="M13_reverse_primer"
     primer_bind     complement(3205..3221)
                     /created_by="primer3"
                     /annotation_group="135473983514354300085f78ef4-0452-4fae-a
                     bba-93b35302c171"
                     /%GC="47.1"
                     /Tm="49.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="M13R"
                     /Transferred_Similarity="100.00%"
                     /label="M13R"
     misc_feature    complement(3218..3240)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="M13_pUC_rev_primer"
     primer_bind     complement(3218..3241)
                     /created_by="primer3"
                     /annotation_group="1354739835389783000b8ada402-17c3-46ef-9
                     adc-177f91dab407"
                     /%GC="45.8"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13-48REV"
                     /Transferred_Similarity="100.00%"
                     /label="M13-48REV"
     promoter        complement(3254..3283)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="lac_promoter"
     primer_bind     complement(3488..3507)
                     /created_by="primer3"
                     /annotation_group="13547385713896020002e37130e-0600-4795-b
                     4cf-db36738ae7a4"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /modified_by="User"
                     /Transferred_From="VR"
                     /Transferred_Similarity="100.00%"
                     /label="VR"
     primer_bind     complement(3594..3613)
                     /Sequence="ATGGAAAAACGCCAGCAACG"
                     /Hairpin_Tm="39.1"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /modified_by="User"
                     /Transferred_From="pUC_ori_sqF"
                     /Transferred_Similarity="100.00%"
                     /label="pUC_ori_sqF"
     gene            complement(4366..5226)
                     /gene="Ampicillin"
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="Ampicillin"
     CDS             complement(4366..5226)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 2"
     primer_bind     complement(4383..4402)
                     /Sequence="CGCTGAGATAGGTGCCTCAC"
                     /Hairpin_Tm="35.7"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /modified_by="User"
                     /Transferred_From="AmpR_3p_sq2"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_3p_sq2"
     primer_bind     5206..5226
                     /created_by="primer3"
                     /annotation_group="5686154009162200c2d23fdc-053e-4e7d-9764
                     -036a2028c4ac"
                     /%GC="33.3"
                     /Tm="53.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACGGAAATGTTGAATACTCAT"
                     /Transferred_From="AmpR_5p_SqR"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_5p_SqR"
     promoter        complement(5268..5296)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catggattac aaggacgacg
     1021 atgacaaggg tggcggtggc tctatggaca ccagccgtgt gcagcctatc aagctggcca
     1081 gggtcaccaa ggtcctgggc aggaccggtt ctcagggaca gtgcacgcag gtgcgcgtgg
     1141 aattcatgga cgacacgagc cgatccatca tccgcaatgt aaaaggcccc gtgcgcgagg
     1201 gcgacgtgct cacccttttg gagtcagagc gagaagcccg gaggttgcgc taggatatcc
     1261 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     1321 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1381 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1441 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1501 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     1561 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     1621 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1681 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1741 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     1801 tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga
     1861 aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     1921 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     1981 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     2041 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     2101 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     2161 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     2221 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     2281 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     2341 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     2401 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     2461 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     2521 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     2581 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     2641 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     2701 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     2761 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     2821 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     2881 cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt
     2941 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3001 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     3061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3121 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     3181 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3241 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3301 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3361 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3421 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3481 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4021 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5341 ccgaaaagtg ccacctgacg tc
//
