LOCUS       RPS28_(modified)        6456 bp    DNA     circular UNA 01-AUG-2022
DEFINITION  RPS28 (modified) PCR Product inserted into psiCHECK-2_miR-27
            (modified) (bases 1785 to 1647).
ACCESSION   urn.local...76-f4q6una
VERSION     urn.local...76-f4q6una
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     misc_feature    15..20
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    54..59
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     promoter        62..419
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /modified_by="User"
                     /label="SV40 promoter"
     misc_feature    311..316
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    350..362
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /annotation_group="GGCCNNNNNGGCC"
                     /label="SfiI"
                     /note="Geneious type: restriction site"
     misc_feature    351..361
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    401..406
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     intron          489..621
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
                     /modified_by="User"
                     /label="chimeric intron"
     promoter        666..684
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /modified_by="User"
                     /label="T7 promoter"
     misc_feature    688..698
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    692..697
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     CDS             694..1629
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /modified_by="User"
                     /label="hRluc"
     misc_feature    1177..1182
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     misc_feature    1353..1358
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI"
                     /note="Geneious type: restriction site"
     misc_feature    1387..1392
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     ligation        1644..1647
                     /label="Ligation"
     misc_feature    1648..1857
                     /Source="referenceToDocument: urn:local:.:br-ej176fj"
                     /label="RPS28 (modified) PCR Product"
                     /note="Geneious type: Inserted sequence"
     gene            <1649..>1855
                     /gene="RPS28"
                     /gene_synonym="DBA15; eS28; S28"
                     /note="ribosomal protein S28"
                     /db_xref="GeneID:6234"
                     /db_xref="HGNC:HGNC:10418"
                     /db_xref="MIM:603685"
                     /label="RPS28 gene"
     source          <1649..>1855
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome=19
                     /map="19p13.2"
                     /label="source Homo sapiens"
     exon            <1649..1664
                     /gene="RPS28"
                     /gene_synonym="DBA15; eS28; S28"
                     /inference="alignment:Splign:2.1.0"
                     /label="RPS28 exon"
     misc_feature    1649..1855
                     /created_by="User"
                     /label="UTR test"
     exon            1665..>1855
                     /gene="RPS28"
                     /gene_synonym="DBA15; eS28; S28"
                     /inference="alignment:Splign:2.1.0"
                     /label="RPS28 exon"
     misc_feature    complement(1694..1726)
                     /created_by="User"
                     /label="RPS_SCR1-2R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1705..1775
                     /created_by="User"
                     /modified_by="User"
                     /label="RNAstructure input (DuplexFold only?)"
     binding_site    complement(1714..1726)
                     /created_by="User"
                     /modified_by="User"
                     /label="Seed 2 SCR"
     primer_bind     1714..1746
                     /created_by="User"
                     /label="RPS_SCR1-2F"
     misc_feature    1715..1726
                     /Original_Bases="AGTCTGCTCCTT"
                     /label="AGTCTGCTCCTT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(1727..1761)
                     /created_by="User"
                     /modified_by="User"
                     /label="RPS_SCR1-1R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1727..1770)
                     /created_by="User"
                     /label="RPS_SCR1-1R2"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1742..1786
                     /created_by="User"
                     /modified_by="User"
                     /label="RPS_SCR1-1F2"
     misc_feature    1747..1760
                     /Original_Bases="ACTGAGATGCTCCT"
                     /label="ACTGAGATGCTCCT"
                     /note="Geneious type: Editing History Replacement"
     binding_site    complement(1747..1761)
                     /created_by="User"
                     /label="Seed 1 SCR"
     primer_bind     1747..1786
                     /created_by="User"
                     /label="RPS_SCR1-1F "
     regulatory      1764..1769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS28"
                     /gene_synonym="DBA15; eS28; S28"
                     /note="hexamer: AATAAA"
                     /label="RPS28 regulatory"
     polyA_site      1789
                     /gene="RPS28"
                     /gene_synonym="DBA15; eS28; S28"
                     /note="major polyA site"
                     /label="RPS28 polyA site"
     misc_feature    1813..1837
                     /created_by="User"
                     /label="rnahybrid binding"
     polyA_site      1815
                     /gene="RPS28"
                     /gene_synonym="DBA15; eS28; S28"
                     /label="RPS28 polyA site"
     ligation        1858..1861
                     /label="Ligation"
     polyA_signal    1871..1919
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="User"
                     /label="poly(A) signal"
     promoter        1928..2679
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /modified_by="User"
                     /label="HSV TK promoter"
     misc_feature    2202..2207
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    2202..2207
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    2212..2222
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    2215..2225
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    2250..2255
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    2569..2574
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    2709..2719
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    2713..2718
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     CDS             2715..4367
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /modified_by="User"
                     /label="Fluc"
     misc_feature    4113..4123
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    4288..4298
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    4372..4377
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     polyA_signal    complement(4411..4532)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="User"
                     /label="SV40 poly(A) signal"
     misc_feature    4530..4535
                     /Recognition_pattern="GTT^AAC"
                     /annotation_group="GTTAAC"
                     /label="HpaI"
                     /note="Geneious type: restriction site"
     misc_feature    4530..4535
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     CDS             complement(4555..4623)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
                     /modified_by="User"
                     /label="large T antigen (fragment)"
     misc_feature    4626..4631
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     misc_feature    4634..4639
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     promoter        4665..4769
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /modified_by="User"
                     /label="AmpR promoter"
     CDS             join(4770..4884,4885..5630)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /modified_by="User"
                     /label="AmpR"
     misc_feature    5433..5443
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     rep_origin      5801..6389
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /modified_by="User"
                     /label="ori"
ORIGIN      
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgaggc ttggctgctc gctgggtctt ggatgtcggg
     1681 ttcgaccact tggccgatgg gaatggtctg tcaccttatt tgcccgtttt ttgtccgcca
     1741 cacgtacctt cggtgaacta ttaaataaag cgtttgtgtt tcaagttaac tcaggttctt
     1801 gtctgggtta tacgactagg gtttctccag gtttcttgag tggctcccag gcggtgcggc
     1861 cgctggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     1921 ggatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
     1981 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
     2041 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
     2101 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
     2161 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
     2221 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
     2281 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
     2341 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
     2401 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
     2461 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     2521 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     2581 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
     2641 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttggcat tccggtactg
     2701 ttggtaaagc caccatggcc gatgctaaga acattaagaa gggccctgct cccttctacc
     2761 ctctggagga tggcaccgct ggcgagcagc tgcacaaggc catgaagagg tatgccctgg
     2821 tgcctggcac cattgccttc accgatgccc acattgaggt ggacatcacc tatgccgagt
     2881 acttcgagat gtctgtgcgc ctggccgagg ccatgaagag gtacggcctg aacaccaacc
     2941 accgcatcgt ggtgtgctct gagaactctc tgcagttctt catgccagtg ctgggcgccc
     3001 tgttcatcgg agtggccgtg gcccctgcta acgacattta caacgagcgc gagctgctga
     3061 acagcatggg catttctcag cctaccgtgg tgttcgtgtc taagaagggc ctgcagaaga
     3121 tcctgaacgt gcagaagaag ctgcctatca tccagaagat catcatcatg gactctaaga
     3181 ccgactacca gggcttccag agcatgtaca cattcgtgac atctcatctg cctcctggct
     3241 tcaacgagta cgacttcgtg ccagagtctt tcgacaggga caaaaccatt gccctgatca
     3301 tgaacagctc tgggtctacc ggcctgccta agggcgtggc cctgcctcat cgcaccgcct
     3361 gtgtgcgctt ctctcacgcc cgcgacccta ttttcggcaa ccagatcatc cccgacaccg
     3421 ctattctgag cgtggtgcca ttccaccacg gcttcggcat gttcaccacc ctgggctacc
     3481 tgatttgcgg ctttcgggtg gtgctgatgt accgcttcga ggaggagctg ttcctgcgca
     3541 gcctgcaaga ctacaaaatt cagtctgccc tgctggtgcc aaccctgttc agcttcttcg
     3601 ctaagagcac cctgatcgac aagtacgacc tgtctaacct gcacgagatt gcctctggcg
     3661 gcgccccact gtctaaggag gtgggcgaag ccgtggccaa gcgctttcat ctgccaggca
     3721 tccgccaggg ctacggcctg accgagacaa ccagcgccat tctgattacc ccagagggcg
     3781 acgacaagcc tggcgccgtg ggcaaggtgg tgccattctt cgaggccaag gtggtggacc
     3841 tggacaccgg caagaccctg ggagtgaacc agcgcggcga gctgtgtgtg cgcggcccta
     3901 tgattatgtc cggctacgtg aataaccctg aggccacaaa cgccctgatc gacaaggacg
     3961 gctggctgca ctctggcgac attgcctact gggacgagga cgagcacttc ttcatcgtgg
     4021 accgcctgaa gtctctgatc aagtacaagg gctaccaggt ggccccagcc gagctggagt
     4081 ctatcctgct gcagcaccct aacattttcg acgccggagt ggccggcctg cccgacgacg
     4141 atgccggcga gctgcctgcc gccgtcgtcg tgctggaaca cggcaagacc atgaccgaga
     4201 aggagatcgt ggactatgtg gccagccagg tgacaaccgc caagaagctg cgcggcggag
     4261 tggtgttcgt ggacgaggtg cccaagggcc tgaccggcaa gctggacgcc cgcaagatcc
     4321 gcgagatcct gatcaaggct aagaaaggcg gcaagatcgc cgtgtaataa ttctagagtc
     4381 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     4441 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4501 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4561 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4621 gtaaaatcga taaggatcca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4921 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4981 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5281 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5941 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6181 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6421 tggccttttg ctggcctttt gctcacatgg ctcgac
//
