LOCUS       Exported                3236 bp ds-DNA     circular SYN 23-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 24, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    188..501
                     
     misc_feature    762..1002
                     
     primer_bind     complement(1015..1031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1039..1055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1063..1093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1108..1129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1417..2005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2176..3036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3037..3141)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggcac
       61 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      121 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
      181 ccatatgttt aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata
      241 ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc
      301 cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta
      361 taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa
      421 ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt attatatata
      481 tattttcttg ttatagatat cgtcacgcgt agcgagtcag tgagcgagga agcggaagag
      541 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      601 ctcgaggaca ggtttcccga ctggaaagcg ggcagcgagt cagtgagcga ggaagcggaa
      661 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      721 cacgacaggt ttcccgactg gaaaggtcga ccggggaaag ttttgttact ttatagaaga
      781 aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa
      841 tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa
      901 taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta
      961 acgtacgtca caatatgatt atctttctag ggttaaaagc ttggcgtaat catggtcata
     1021 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1081 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1141 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1201 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1621 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1681 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1741 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1801 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1861 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1921 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1981 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2041 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2101 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2161 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2221 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2281 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2341 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2401 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2461 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2521 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2581 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2641 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2701 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2761 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2821 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2881 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2941 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3001 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3061 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3121 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3181 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
