LOCUS       pAAV-CMV-CasRx-VEGFA_ar 7310 bp ds-DNA     circular     29-MAY-2023
DEFINITION  .
KEYWORDS    "accession:VB201208-1189rrh" "marker:5' ITR, 3' ITR"
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="5' ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        169..409
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        169..1270
                     /label="U6 VEGF sgRNA array-CMV"
                     /ApEinfo_revcolor="#c54b7c"
                     /ApEinfo_fwdcolor="#c54b7c"
     misc_RNA        419..454
                     /label="CasRx DR (full)"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_RNA        complement(455..484)
                     /label="30nt VEGFA-1 presgRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_RNA        485..520
                     /label="CasRx DR (full)"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_RNA        complement(521..550)
                     /label="30nt VEGFA-2 presgRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_RNA        551..586
                     /label="CasRx DR (full)"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_RNA        complement(587..616)
                     /label="30nt VEGFA-3 presgRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_RNA        617..652
                     /label="CasRx DR (full)"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     promoter        682..1270
                     /label="CMV"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1295..1300
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1301..4243
                     /label="CasRx"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             1301..4303
                     /label="CasRx"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1301..4303
                     /label="Translation 1301-4303"
                     /translation="MSPKKKRKVEASIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSIRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEKRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVYTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIINYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKDMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVINLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAGRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFFKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDENGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQKKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRENAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEEKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKAEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHAYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVPFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNSGSGPKKKRKVAAAYPYDVPDYA*"
     CDS             4244..4264
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4274..4300
                     /label="HA"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     polyA_signal    4358..4565
                     /label="BGH pA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4573..4713)
                     /label="3' ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5630..6490
                     /label="AmpiciIIin"
                     /ApEinfo_revcolor="#da3bb5"
                     /ApEinfo_fwdcolor="#da3bb5"
     rep_origin      6661..7249
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgga gggcctattt
      181 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
      241 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      301 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      361 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgca
      421 agtaaacccc taccaactgg tcggggtttg aaactatgtg ctggccttgg tgaggtttga
      481 tccgcaagta aacccctacc aactggtcgg ggtttgaaac cacttcgtga tgattctgcc
      541 ctcctccttc caagtaaacc cctaccaact ggtcggggtt tgaaactctg cattcacatt
      601 tgttgtgctg taggaacaag taaaccccta ccaactggtc ggggtttgaa actttttttg
      661 aattctgatg cggtgaagct ttagttatta atagtaatca attacggggt cattagttca
      721 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      781 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      841 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      901 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      961 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1021 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1081 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1141 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1201 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     1261 ccgtcagatc caagtttgta caaaaaagca ggctgccacc atgagcccca agaagaagag
     1321 aaaggtggag gccagcatcg aaaaaaaaaa gtccttcgcc aagggcatgg gcgtgaagtc
     1381 cacactcgtg tccggctcca aagtgtacat gacaaccttc gccgaaggca gcgacgccag
     1441 gctggaaaag atcgtggagg gcgacagcat caggagcgtg aatgagggcg aggccttcag
     1501 cgctgaaatg gccgataaaa acgccggcta taagatcggc aacgccaaat tcagccatcc
     1561 taagggctac gccgtggtgg ctaacaaccc tctgtataca ggacccgtcc agcaggatat
     1621 gctcggcctg aaggaaactc tggaaaagag gtacttcggc gagagcgctg atggcaatga
     1681 caatatttgt atccaggtga tccataacat cctggacatt gaaaaaatcc tcgccgaata
     1741 cattaccaac gccgcctacg ccgtcaacaa tatctccggc ctggataagg acattattgg
     1801 attcggcaag ttctccacag tgtataccta cgacgaattc aaagaccccg agcaccatag
     1861 ggccgctttc aacaataacg ataagctcat caacgccatc aaggcccagt atgacgagtt
     1921 cgacaacttc ctcgataacc ccagactcgg ctatttcggc caggcctttt tcagcaagga
     1981 gggcagaaat tacatcatca attacggcaa cgaatgctat gacattctgg ccctcctgag
     2041 cggactgagg cactgggtgg tccataacaa cgaagaagag tccaggatct ccaggacctg
     2101 gctctacaac ctcgataaga acctcgacaa cgaatacatc tccaccctca actacctcta
     2161 cgacaggatc accaatgagc tgaccaactc cttctccaag aactccgccg ccaacgtgaa
     2221 ctatattgcc gaaactctgg gaatcaaccc tgccgaattc gccgaacaat atttcagatt
     2281 cagcattatg aaagagcaga aaaacctcgg attcaatatc accaagctca gggaagtgat
     2341 gctggacagg aaggatatgt ccgagatcag gaaaaatcat aaggtgttcg actccatcag
     2401 gaccaaggtc tacaccatga tggactttgt gatttatagg tattacatcg aagaggatgc
     2461 caaggtggct gccgccaata agtccctccc cgataatgag aagtccctga gcgagaagga
     2521 tatctttgtg attaacctga ggggctcctt caacgacgac cagaaggatg ccctctacta
     2581 cgatgaagct aatagaattt ggagaaagct cgaaaatatc atgcacaaca tcaaggaatt
     2641 taggggaaac aagacaagag agtataagaa gaaggacgcc cctagactgc ccagaatcct
     2701 gcccgctggc cgtgatgttt ccgccttcag caaactcatg tatgccctga ccatgttcct
     2761 ggatggcaag gagatcaacg acctcctgac caccctgatt aataaattcg ataacatcca
     2821 gagcttcctg aaggtgatgc ctctcatcgg agtcaacgct aagttcgtgg aggaatacgc
     2881 ctttttcaaa gactccgcca agatcgccga tgagctgagg ctgatcaagt ccttcgctag
     2941 aatgggagaa cctattgccg atgccaggag ggccatgtat atcgacgcca tccgtatttt
     3001 aggaaccaac ctgtcctatg atgagctcaa ggccctcgcc gacacctttt ccctggacga
     3061 gaacggaaac aagctcaaga aaggcaagca cggcatgaga aatttcatta ttaataacgt
     3121 gatcagcaat aaaaggttcc actacctgat cagatacggt gatcctgccc acctccatga
     3181 gatcgccaaa aacgaggccg tggtgaagtt cgtgctcggc aggatcgctg acatccagaa
     3241 aaaacagggc cagaacggca agaaccagat cgacaggtac tacgaaactt gtatcggaaa
     3301 ggataagggc aagagcgtga gcgaaaaggt ggacgctctc acaaagatca tcaccggaat
     3361 gaactacgac caattcgaca agaaaaggag cgtcattgag gacaccggca gggaaaacgc
     3421 cgagagggag aagtttaaaa agatcatcag cctgtacctc accgtgatct accacatcct
     3481 caagaatatt gtcaatatca acgccaggta cgtcatcgga ttccattgcg tcgagcgtga
     3541 tgctcaactg tacaaggaga aaggctacga catcaatctc aagaaactgg aagagaaggg
     3601 attcagctcc gtcaccaagc tctgcgctgg cattgatgaa actgcccccg ataagagaaa
     3661 ggacgtggaa aaggagatgg ctgaaagagc caaggagagc attgacagcc tcgagagcgc
     3721 caaccccaag ctgtatgcca attacatcaa atacagcgac gagaagaaag ccgaggagtt
     3781 caccaggcag attaacaggg agaaggccaa aaccgccctg aacgcctacc tgaggaacac
     3841 caagtggaat gtgatcatca gggaggacct cctgagaatt gacaacaaga catgtaccct
     3901 gttcagaaac aaggccgtcc acctggaagt ggccaggtat gtccacgcct atatcaacga
     3961 cattgccgag gtcaattcct acttccaact gtaccattac atcatgcaga gaattatcat
     4021 gaatgagagg tacgagaaaa gcagcggaaa ggtgtccgag tacttcgacg ctgtgaatga
     4081 cgagaagaag tacaacgata ggctcctgaa actgctgtgt gtgcctttcg gctactgtat
     4141 ccccaggttt aagaacctga gcatcgaggc cctgttcgat aggaacgagg ccgccaagtt
     4201 cgacaaggag aaaaagaagg tgtccggcaa ttccggatcc ggacctaaga aaaagaggaa
     4261 ggtggcggcc gcttacccat acgatgttcc agattacgct taaacccagc tttcttgtac
     4321 aaagtgggaa ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     4381 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4441 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4501 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct
     4561 ggggagggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4621 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4681 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4741 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4801 cggcgcatta agcgcggcgg gggtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4861 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4921 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4981 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     5041 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5101 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     5161 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5221 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     5281 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5341 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5401 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5461 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5521 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5581 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5641 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5701 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5761 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5821 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5881 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5941 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6001 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6061 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6121 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6181 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6241 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6301 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
     6361 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6421 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6481 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//