LOCUS       pAAV-CMV-Cas13X.1_(IDT) 6710 bp ds-DNA     circular     05-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="5' ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        169..409
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        169..1270
                     /label="U6 VEGF sgRNA array-CMV"
                     /ApEinfo_revcolor="#c54b7c"
                     /ApEinfo_fwdcolor="#c54b7c"
     misc_feature    419..454
                     /label="CasX.1 DR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_RNA        complement(455..484)
                     /label="30nt VEGFA-1 presgRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    485..520
                     /label="CasX.1 DR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_RNA        complement(521..550)
                     /label="30nt VEGFA-2 presgRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    551..586
                     /label="CasX.1 DR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_RNA        complement(587..616)
                     /label="30nt VEGFA-3 presgRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    617..652
                     /label="CasX.1 DR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    653..659
                     /label="Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        682..1270
                     /label="CMV"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1295..1300
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1301..3703
                     /label="Cas13X.1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1301..3703
                     /label="Translation 1301-3703"
                     /translation="MPKKKRKVAQVSKQTSKKRELSIDEYQGARKWCFTIAFNKALVNRDKNDGLFVESLLRHEKYSKHDWYDEDTRALIKCSTQAANAKAEALRNYFSHYRHSPGCLTFTAEDELRTIMERAYERAIFECRRRETEVIIEFPSLFEGDRITTAGVVFFVSFFVERRVLDRLYGAVSGLKKNEGQYKLTRKALSMYCLKDSRFTKAWDKRVLLFRDILAQLGRIPAEAYEYYHGEQGDKKRANDNEGTNPKRHKDKFIEFALHYLEAQHSEICFGRRHIVREEAGAGDEHKKHRTKGKVVVDFSKKDEDQSYYISKNNVIVRIDKNAGPRSYRMGLNELKYLVLLSLQGKGDDAIAKLYRYRQHVENILDVVKVTDKDNHVFLPRFVLEQHGIGRKAFKQRIDGRVKHVRGVWEKKKAATNEMTLHEKARDILQYVNENCTRSFNPGEYNRLLVCLVGKDVENFQAGLKRLQLAERIDGRVYSIFAQTSTINEMHQVVCDQILNRLCRIGDQKLYDYVGLGKKDEIDYKQKVAWFKEHISIRRGFLRKKFWYDSKKGFAKLVEEHLESGGGQRDVGLDKKYYHIDAIGRFEGANPALYETLARDRLCLMMAQYFLGSVRKELGNKIVWSNDSIELPVEGSVGNEKSIVFSVSDYGKLYVLDDAEFLGRICEYFMPHEKGKIRYHTVYEKGFRAYNDLQKKCVEAVLAFEEKVVKAKKMSEKEGAHYIDFREILAQTMCKEAEKTAVNKVRRAFFHHHLKFVIDEFGLFSDVMKKYGIEKEWKFPVKPKKKRKVSSYPYDVPDYA*"
     misc_feature    1304..1324
                     /label="NLS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3647..3667
                     /label="NLS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3674..3700
                     /label="HA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     polyA_signal    3758..3965
                     /label="BGH pA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3973..4113)
                     /label="3' ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5030..5890
                     /label="AmpiciIIin"
                     /ApEinfo_revcolor="#da3bb5"
                     /ApEinfo_fwdcolor="#da3bb5"
     rep_origin      6061..6649
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgga gggcctattt
      181 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
      241 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      301 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      361 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgGC
      421 TGGAGCAGCC CCCGATTTGT GGGGTGATTA CAGCtatgtg ctggccttgg tgaggtttga
      481 tccgGCTGGA GCAGCCCCCG ATTTGTGGGG TGATTACAGC cacttcgtga tgattctgcc
      541 ctcctccttc GCTGGAGCAG CCCCCGATTT GTGGGGTGAT TACAGCtctg cattcacatt
      601 tgttgtgctg taggaaGCTG GAGCAGCCCC CGATTTGTGG GGTGATTACA GCtttttttg
      661 aattctgatg cggtgaagct ttagttatta atagtaatca attacggggt cattagttca
      721 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      781 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      841 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      901 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      961 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1021 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1081 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1141 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1201 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     1261 ccgtcagatc caagtttgta caaaaaagca ggctgccacc ATGCCAAAGA AGAAACGGAA
     1321 GGTCGCTCAG GTCTCTAAGC AGACATCCAA GAAGAGGGAA CTTTCCATTG ATGAATATCA
     1381 AGGTGCCAGA AAATGGTGTT TTACAATCGC CTTTAATAAG GCCTTGGTCA ATCGGGACAA
     1441 GAATGACGGA CTTTTCGTTG AATCCCTTCT CCGCCACGAA AAATACTCCA AGCATGATTG
     1501 GTACGATGAG GACACCAGGG CATTGATCAA ATGTAGCACA CAAGCCGCAA ATGCTAAGGC
     1561 AGAGGCTCTT CGAAATTACT TCTCACATTA CAGGCACAGT CCAGGGTGCT TGACCTTCAC
     1621 CGCAGAGGAC GAGTTGCGGA CTATTATGGA GCGGGCATAC GAAAGGGCCA TATTCGAATG
     1681 TAGACGACGC GAAACAGAAG TCATTATTGA GTTCCCCTCA CTGTTCGAGG GCGATAGGAT
     1741 AACTACAGCT GGGGTAGTCT TTTTTGTCTC TTTCTTCGTT GAAAGACGAG TGCTCGACCG
     1801 ACTCTACGGA GCCGTGAGCG GTCTGAAAAA GAATGAGGGC CAATACAAAC TCACCCGAAA
     1861 GGCACTCTCC ATGTACTGTC TTAAGGATTC CCGATTTACC AAGGCATGGG ACAAAAGGGT
     1921 ATTGTTGTTC AGGGATATCC TGGCACAGTT GGGGCGAATT CCCGCCGAAG CATATGAATA
     1981 CTACCACGGC GAACAGGGTG ATAAAAAACG CGCTAATGAT AACGAGGGCA CAAATCCAAA
     2041 GCGCCATAAA GACAAATTCA TTGAATTCGC ATTGCATTAT CTTGAAGCCC AGCACAGCGA
     2101 AATCTGCTTC GGTAGGCGCC ACATTGTACG AGAAGAGGCT GGTGCTGGAG ATGAACATAA
     2161 GAAGCACCGC ACCAAGGGGA AGGTAGTTGT CGATTTTAGT AAGAAAGATG AAGACCAGTC
     2221 ATATTACATA TCCAAGAATA ACGTAATTGT GCGCATTGAT AAGAACGCCG GACCACGCTC
     2281 TTATAGAATG GGGCTGAATG AGCTCAAGTA CTTGGTCTTG CTTAGCCTCC AGGGAAAAGG
     2341 GGATGACGCT ATTGCTAAGC TCTATCGCTA TAGACAACAC GTTGAGAATA TCCTCGACGT
     2401 TGTGAAAGTT ACAGATAAGG ATAATCATGT ATTCCTGCCT AGATTTGTGT TGGAACAGCA
     2461 CGGCATTGGT AGGAAGGCAT TCAAGCAAAG GATCGATGGA AGAGTCAAGC ATGTGCGCGG
     2521 AGTGTGGGAA AAAAAAAAAG CAGCCACTAA CGAGATGACC CTGCACGAAA AAGCTAGGGA
     2581 TATCCTTCAG TATGTAAACG AAAACTGTAC TCGCTCCTTT AATCCTGGAG AATACAATAG
     2641 ACTGCTTGTG TGCCTTGTTG GGAAGGATGT AGAGAACTTT CAAGCCGGCC TTAAGCGACT
     2701 CCAGTTGGCT GAACGGATAG ATGGACGAGT GTATAGTATA TTCGCACAGA CTTCTACAAT
     2761 CAATGAAATG CATCAAGTCG TTTGTGACCA AATTTTGAAC AGGCTTTGCA GGATCGGTGA
     2821 TCAAAAGCTC TATGACTATG TCGGGCTGGG TAAGAAGGAT GAAATAGATT ACAAACAGAA
     2881 GGTCGCCTGG TTTAAGGAGC ACATTTCCAT CAGACGGGGG TTTTTGCGGA AGAAATTTTG
     2941 GTACGATTCT AAAAAAGGAT TTGCAAAGCT GGTGGAAGAA CATTTGGAGA GCGGAGGCGG
     3001 GCAGCGCGAC GTAGGTCTTG ATAAGAAATA CTACCACATA GACGCAATTG GGCGGTTCGA
     3061 GGGAGCAAAC CCTGCCTTGT ATGAAACCCT CGCCCGAGAT AGATTGTGCT TGATGATGGC
     3121 ACAATATTTT CTGGGTTCAG TACGGAAGGA ACTTGGTAAC AAGATTGTTT GGAGCAATGA
     3181 CAGCATCGAA TTGCCTGTTG AAGGGAGTGT CGGTAATGAA AAGTCCATTG TATTTTCCGT
     3241 GAGTGACTAT GGCAAGTTGT ATGTTCTTGA CGATGCAGAA TTCCTTGGGC GAATCTGCGA
     3301 GTACTTCATG CCCCACGAAA AGGGTAAAAT ACGCTATCAT ACAGTTTATG AGAAAGGCTT
     3361 TCGAGCATAT AATGACCTGC AAAAGAAGTG TGTCGAAGCA GTCCTTGCTT TCGAAGAAAA
     3421 GGTCGTAAAA GCAAAGAAGA TGTCCGAAAA AGAGGGCGCC CATTACATTG ACTTCAGAGA
     3481 GATCCTTGCC CAGACAATGT GTAAGGAAGC CGAGAAAACT GCTGTGAATA AGGTAAGGAG
     3541 AGCCTTTTTC CATCACCACC TCAAGTTCGT CATAGACGAA TTTGGATTGT TTTCTGATGT
     3601 GATGAAAAAA TATGGGATTG AGAAAGAATG GAAGTTTCCA GTGAAGCCCA AGAAAAAAAG
     3661 AAAAGTATCC TCCtacccat acgatgttcc agattacgct TGAacccagc tttcttgtac
     3721 aaagtgggaa ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     3781 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3841 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3901 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct
     3961 ggggagggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4021 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4081 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4141 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4201 cggcgcatta agcgcggcgg gggtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4261 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4381 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4501 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     4561 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4621 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4681 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4741 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4801 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4861 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4921 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4981 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5581 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5641 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
     5761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5881 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6061 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6181 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//