LOCUS       pAG416GAL10-WNV_        6668 bp DNA     circular SYN 29-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2023 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             771..864
                     /locus_tag="lacI"
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
     protein_bind    complement(877..898)
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     promoter        913..943
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(947..969)
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     promoter        1012..1030
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        complement(1101..1753)
                     /locus_tag="GAL10 promoter"
                     /label=GAL10 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=97.444"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1765..1789
                     /locus_tag="YPet"
                     /label=YPet
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5161744022503516"
                     /note="/other=CDS"
     misc_feature    1827..1851
                     /locus_tag="attB1"
                     /label=attB1
     misc_feature    1857..2597
                     /locus_tag="WNV NS2B-GS-NS3"
                     /label=WNV NS2B-GS-NS3
     misc_feature    1860..2015
                     /locus_tag="NS2B 45-96"
                     /label=NS2B 45-96
     misc_feature    2016..2042
                     /locus_tag="GS linker"
                     /label=GS linker
     misc_feature    2043..2594
                     /locus_tag="NS3 1-184"
                     /label=NS3 1-184
     misc_feature    2599..2623
                     /locus_tag="attB2"
                     /label=attB2
     misc_feature    2627..2674
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=45.28301886792453"
                     /note="/other=misc feature"
     terminator      2675..2922
                     /locus_tag="CYC1 terminator"
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     promoter        complement(2941..2959)
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2967..3125
                     /locus_tag="lacZ"
                     /label=lacZ
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.170731707317073"
                     /note="/other=CDS"
     rep_origin      3126..3581
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(3582..3634)
                     /locus_tag="bom"
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.1294964028777"
                     /note="/other=misc feature"
     CDS             complement(3712..4515)
                     /locus_tag="URA3"
                     /label=URA3
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(4516..4731)
                     /locus_tag="URA3 promoter"
                     /label=URA3 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(4739..4788)
                     /locus_tag="bom(1)"
                     /label=bom
                     /label=bom(1)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.97122302158273"
                     /note="/other=misc feature"
     CDS             complement(4890..4925)
                     /locus_tag="rop"
                     /label=rop
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.047619047619047"
                     /note="/other=CDS"
     misc_feature    4993..5496
                     /locus_tag="CEN/ARS"
                     /label=CEN/ARS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.802"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     promoter        5533..5637
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5638..6498
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcta tgaccatgat tacgccaagc ggggtgtgtt
     1081 gttggtgggt tgggtgtata gttttttctc cttgacgtta aagtatagag gtatattaac
     1141 aattttttgt tgatactttt atgacatttg aataagaagt aatacaaact gaaaatgttg
     1201 aaagtattag ttaaagtggt tatgcagctt ttccatttat atatctgtta atagatcaaa
     1261 aatcatcgct tcgctgatta attaccccag aaataaggct aaaaaactaa tcgcattatc
     1321 atcctatggt tgttaatttg attcgttaat ttgaaggttt gtggggccag gttactgcca
     1381 atttttcctc ttcataacca taaaagctag tattgtagaa tctttattgt tcggagcagt
     1441 gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg acgcacggag
     1501 gagagtcttc cgtcggaggg ctgtcgcccg ctcggcggct tctaatccgt acttcaatat
     1561 agcaatgagc agttaagcgt attactgaaa gttccaaaga gaaggttttt ttaggctaag
     1621 ataatggggc tctttacatt tccacaacat ataagtaaga ttagatatgg atatgtatat
     1681 ggtggtaatg ccatgtaata tgattattaa acttctttgc gtccatccaa aaaaaaagta
     1741 agaatttttg aaaattcaat ataaatgtct aaaggtgaag aattattcag aattccagct
     1801 gaccaccgac tagtggatcc cccatcacaa gtttgtacaa aaaagcaggc tacaaaatga
     1861 tctctggaaa gtcaacagac atgtggattg agaggacggc tgacattact tgggagagtg
     1921 atgctgaaat cacaggctct agcgaaagag tagatgtgag gctggatgat gatggaaatt
     1981 ttcaactgat gaatgacccc ggggcaccat ggaaaggagg tggaggttca ggggggggtg
     2041 gaggaggtgt tctttgggac acaccatcac ccaaggagta caagaagggt gataccacca
     2101 ctggcgttta cagaatcatg actcgaggtc tgcttggcag ttaccaagct ggagccggag
     2161 tgatggtaga gggggtgttc cacacactat ggcacaccac taagggagct gctctcatga
     2221 gtggtgaggg acgtctggat ccctactggg ggagcgtgaa agaggaccga ctttgctatg
     2281 gggggccatg gaaactccaa cataaatgga atggacatga tgaggtccaa atgattgtcg
     2341 tggagccagg gaaaaatgtg aaaaacgtcc agaccaagcc cggagtgttt aagacaccag
     2401 aaggagaaat tggggcagtt acgctagact atcctaccgg aacgtcaggt tcccccattg
     2461 tagacaaaaa tggagatgtg attggattgt atgggaacgg cgtcatcatg cctaatggtt
     2521 catacataag cgccattgtg caaggagaga gaatggaaga accggcacca gctggcttcg
     2581 aacctgaaat gttgtaaaac ccagctttct tgtactaagt ggtgatgggc tgcaggaatt
     2641 cgatatcaag cttatcgata ccgtcgacct cgagtcatgt aattagttat gtcacgctta
     2701 cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa
     2761 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
     2821 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
     2881 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggta cccaattcgc
     2941 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     3001 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3061 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3121 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3541 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     3601 cttacgcatc tgtgcggtat ttcacaccgc atagggtaat aactgatata attaaattga
     3661 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
     3721 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
     3781 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
     3841 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
     3901 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
     3961 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
     4021 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
     4081 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
     4141 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
     4201 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
     4261 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
     4321 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
     4381 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
     4441 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
     4501 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
     4561 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
     4621 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
     4681 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta
     4741 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4801 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4861 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4921 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4981 gtttcttaga cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga
     5041 ataatttggg aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa
     5101 agtaaataaa gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa
     5161 aaaatttcaa caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa
     5221 atagatatac attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg
     5281 tcttctacac agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag
     5341 ataaaaggta gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa
     5401 actatttttt ctttaatttc tttttttact ttctattttt aatttatata tttatattaa
     5461 aaaatttaaa ttataattat ttttatagca cgtgatgaaa aggacccagg tggcactttt
     5521 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5581 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5641 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5701 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5761 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5821 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5881 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5941 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6001 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6061 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6121 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6181 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6241 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6301 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6361 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6421 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6481 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6541 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6601 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6661 ggatcttc
//
