LOCUS       FB312                   7902 bp ds-DNA     linear       30-MAY-2023
DEFINITION  (GB_UD_2B0D,GB_UD_CB06,GB_UD_CB1D)pDGB3_alpha1R.
ACCESSION   GB_UA_6DA4
VERSION     GB_UA_6DA4
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     domest_feature  complement(1..274)
                     /locus_tag="Ttub"
                     /label="Ttub"
                     /ApEinfo_label="Ttub"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     domest_feature  complement(279..794)
                     /locus_tag="Nluc CDS"
                     /label="Nluc CDS"
                     /ApEinfo_label="Nluc CDS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(796..1523)
                     /locus_tag="PgpdA"
                     /label="PgpdA"
                     /ApEinfo_label="PgpdA"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1544..1568
                     /vntifkey="21"
                     /locus_tag="RB"
                     /label="RB"
                     /ApEinfo_label="RB"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7853..7878
                     /vntifkey="21"
                     /locus_tag="LB"
                     /label="LB"
                     /ApEinfo_label="LB"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6608..7420)
                     /vntifkey="4"
                     /note="kanamycin resistance gene"
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6033..6313)
                     /vntifkey="33"
                     /note="pBR322 origin of replication"
                     /locus_tag="pBR322\ori"
                     /label="pBR322\ori"
                     /ApEinfo_label="pBR322\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5633..5893)
                     /vntifkey="21"
                     /note="bom site from pBR322"
                     /locus_tag="pBR322"
                     /label="pBR322"
                     /ApEinfo_label="pBR322"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2630..3630)
                     /vntifkey="21"
                     /note="STA region from pVS1 plasmid"
                     /locus_tag="STA\region\from\pVS1\plasmid"
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4223..5223)
                     /vntifkey="21"
                     /note="pVS1-REP; replication origin from pVS1"
                     /locus_tag="pVS1-Rep\ori"
                     /label="pVS1-Rep\ori"
                     /ApEinfo_label="pVS1-Rep\ori"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcatcatgca acatgcatgt actgtctgat gtattaagag tataggggtc aacaactaga
       61 gtgtctgaca ggtatatcta gcagtttgat ttgaccattc ttttattaaa acactgtaac
      121 ccgcaacgaa gaactcctcc gcttcaagtc aagaatgcgc atatgtgcgt aaaatcgaca
      181 agcccggcgg cattccaccg agcagggtat tgcctttggc actttacgtg cgcagaggag
      241 cctgaatgtt gagtggaatg atggtggaat gatgaagctt atgcgagaat acgttcgcaa
      301 aggcgccaac cagtaactcc gttaatggta acacgaaaca gcaaggagcc atcagggttg
      361 atgaggcgct catcgataat cttgtttcca ttccacaaag taccggtcac agtaatcttc
      421 ttaccgtcga acacagcgat accctcgtag ggtcggccga agtagtcaat catgttgggg
      481 gtgacgccat cgatcacaag agtaccgtag tgcaggataa ctttaaagtg atgatcgtcg
      541 actggataaa cgaccttgaa aattttctcg atctgtccca tttgatcgcc agagaggccc
      601 tcgtatggga taatgacgtg gatgtcaatc ttaagaccgt tctctccgct caacacaata
      661 cgctggatag gagtaacgga aacgccgaga ttctggaaca aggaagagac tcctccctgc
      721 tccagcacct ggtcaaggtt atatccggcg gtttggcgcc agtcgccgac gaaatcttca
      781 agggtgaaaa ccattttaaa ggttcttgga tgggaagatg aatatactga agatgggaaa
      841 agaaagagaa aagaaaagag cagctggtgg ggagagcagg aaaatatggc aacaaatgtt
      901 ggactgacgc aacgaccttg tcaaccccgc cgacacaccg ggcggacaga cggggcaaag
      961 ctgcctacca gggactgagg gacctcagca ggtcgagtgc agagcaccgg atgggtcgac
     1021 tgccagcttg tgttcccggt ctgcgccgct ggccagctcc tgagcggcct ttccggtttc
     1081 atacaccggg caaagcagga gaggcacgat atttggacgc cctacagatg ccggatgggc
     1141 caattaggga gcttacgcgc cgggtactcg ctctacctac ttcggagaag gtactatctc
     1201 gtgaatcttt taccagatcg gaagcaattg gacttctgta cctaggttaa tggcatgcta
     1261 tttcgccgac ggctatacac ccctggcttc acattctcct tcgcttactg ccggtgattc
     1321 gatgaagctc catattctcc gatgatgcaa tagattcttg gtcaacgagg ggcacaccag
     1381 cctttccact tcggggcgga ggggcggccg gtcccggatt aataatcatc cactgcacct
     1441 cagagccgcc agagctgtct ggcgcagtgg cgcttattac tcagcccttc tctctgcgtc
     1501 cgtccgtctg tccgcatgcg ccactccgtc atgagacgga tcctgacagg atatattggc
     1561 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     1621 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     1681 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     1741 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     1801 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     1861 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     1921 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     1981 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     2041 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     2101 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     2161 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     2221 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     2281 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     2341 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     2401 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     2461 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     2521 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     2581 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     2641 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc
     2701 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     2761 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     2821 tgcgtatatg atccgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     2881 gctgtactta accagaaagg cgggtcaggc aagacgacca tcggaaccca tctagcccgc
     2941 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     3001 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     3061 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     3121 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     3181 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     3241 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     3301 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     3361 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     3421 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     3481 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     3541 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     3601 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     3661 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tagatcgcgc
     3721 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     3781 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     3841 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     3901 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat
     3961 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     4021 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     4081 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     4141 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     4201 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     4261 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     4321 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     4381 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     4441 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     4501 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     4561 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     4621 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     4681 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     4741 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     4801 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     4861 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     4921 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     4981 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     5041 aaaaggtcga aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     5101 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     5161 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     5221 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     5281 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     5341 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     5401 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     5461 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga
     5521 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5581 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     5641 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     5701 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     5761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6361 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     6601 gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     6661 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     6721 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     6781 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     6841 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     6901 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     6961 attgcgcctg agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     7021 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     7081 gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg
     7141 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     7201 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     7261 gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc
     7321 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     7381 gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacagaactt attatttcct
     7441 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     7501 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     7561 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     7621 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     7681 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     7741 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     7801 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     7861 tatattgtgg tgtaaacata acggatccgt ctcaggagag cg 
//
