LOCUS       pPSt                    4534 bp ds-DNA     circular     29-OCT-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..17
                     /label="M13 fwd"
     promoter        27..45
                     /label="T7 promoter"
     primer_bind     71..87
                     /label="KS primer"
     misc_feature    109..148
                     /label="BbsI cassette for enhancer+promoter+UTR+bc"
     misc_feature    145..182
                     /label="BsaI cassette for GFP"
     misc_feature    179..216
                     /label="Esp3I cassette for terminator"
     promoter        complement(283..301)
                     /label="T3 promoter"
     primer_bind     complement(322..338)
                     /label="M13 rev"
     protein_bind    346..362
                     /label="lac operator"
     promoter        complement(370..400)
                     /label="lac promoter"
     protein_bind    415..436
                     /label="CAP binding site"
     misc_feature    675..699
                     /label="RB T-DNA repeat"
     rep_origin      complement(790..1378)
                     /label="ori"
     CDS             complement(1549..2337)
                     /label="SmR"
     rep_origin      2628..3063
                     /label="pSa ori"
     misc_feature    3198..3220
                     /label="LB T-DNA repeat"
     terminator      3244..3496
                     /label="NOS terminator"
     CDS             complement(3516..4067)
                     /label="BlpR"
     promoter        complement(4109..4288)
                     /label="NOS promoter"
ORIGIN
        1 GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG
       61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCGC TGCCGTCTTC
      121 AGTGCCTGCA GGCATAGAAG ACCCGTGACG AGACCACAAG GCGCGCCTAG TGGTCTCCTA
      181 AGGGAGACGG GATCCTACAG CTAGCCGTCT CGAGGTGTTC CACTTCTGTA TAGGAGCCAA
      241 TTGATTGACA ACATGCATCA ATCGGAGCTC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT
      301 TGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
      361 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
      421 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
      481 GTGCCAGCTG CATTAATGAA TCGGCCAACG CTCGGGGAGA GGCGGTTTGC GTATTGGGCG
      541 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
      601 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
      661 GAACATGAAG GCCTTGACAG GATATATTGG CGGGTAAACT AAGTCGCTGT ATGTGTTTGT
      721 TTGAGATCTC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
      781 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
      841 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
      901 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
      961 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1021 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1081 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1141 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1201 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1261 GCCAGTTACC TTCGGAAGAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1321 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1381 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     1441 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     1501 AAGTTTTAAA TCAATCTAAA GTATATATGT GTAACATTGG TCTAGTGATT ATTTGCCGAC
     1561 TACCTTGGTG ATCTCGCCTT TCACGTAGTG AACAAATTCT TCCAACTGAT CTGCGCGCGA
     1621 GGCCAAGCGA TCTTCTTGTC CAAGATAAGC CTGCCTAGCT TCAAGTATGA CGGGCTGATA
     1681 CTGGGCCGGC AGGCGCTCCA TTGCCCAGTC GGCAGCGACA TCCTTCGGCG CGATTTTGCC
     1741 GGTTACTGCG CTGTACCAAA TGCGGGACAA CGTAAGCACT ACATTTCGCT CATCGCCAGC
     1801 CCAGTCGGGC GGCGAGTTCC ATAGCGTTAA GGTTTCATTT AGCGCCTCAA ATAGATCCTG
     1861 TTCAGGAACC GGATCAAAGA GTTCCTCCGC CGCTGGACCT ACCAAGGCAA CGCTATGTTC
     1921 TCTTGCTTTT GTCAGCAAGA TAGCCAGATC AATGTCGATC GTGGCTGGCT CGAAGATACC
     1981 TGCAAGAATG TCATTGCGCT GCCATTCTCC AAATTGCAGT TCGCGCTTAG CTGGATAACG
     2041 CCACGGAATG ATGTCGTCGT GCACAACAAT GGTGACTTCT ACAGCGCGGA GAATCTCGCT
     2101 CTCTCCAGGG GAAGCCGAAG TTTCCAAAAG GTCGTTGATC AAAGCTCGCC GCGTTGTTTC
     2161 ATCAAGCCTT ACGGTCACCG TAACCAGCAA ATCAATATCA CTGTGTGGCT TCAGGCCGCC
     2221 ATCCACTGCG GAGCCGTACA AATGTACGGC CAGCAACGTC GGTTCGAGAT GGCGCTCGAT
     2281 GACGCCAACT ACCTCTGATA GTTGAGTCGA TACTTCGGCG ATCACCGCTT CCCTCATAAC
     2341 ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGATG ATATATTTTT
     2401 ATCTTGTGCA ATGTAACATC AGAGATTTTG AGACACAACG TGGCTTTGTT GAATAAATCG
     2461 AACTTTTGCT GAGTTGAAGG ATCAGATCAC GCATCTTCCC GACAACGCAG ACCGTTCCGT
     2521 GGCAAAGCAA AAGTTCAAAA TCACCAACTG GTCCACCTAC AACAAAGCTC TCATCAACCG
     2581 TGGCTCCCTC ACTTTCTGGC TGGATGATGG GGCGATTCAG GCGATCCCCA TCCAACAGCC
     2641 CGCCGTCGAG CGGGCTTTTT TATCCCCGGA AGCCTGTGGA TAGAGGGTAG TTATCCACGT
     2701 GAAACCGCTA ATGCCCCGCA AAGCCTTGAT TCACGGGGCT TTCCGGCCCG CTCCAAAAAC
     2761 TATCCACGTG AAATCGCTAA TCAGGGTACG TGAAATCGCT AATCGGAGTA CGTGAAATCG
     2821 CTAATAAGGT CACGTGAAAT CGCTAATCAA AAAGGCACGT GAGAACGCTA ATAGCCCTTT
     2881 CAGATCAACA GCTTGCAAAC ACCCCTCGCT CCGGCAAGTA GTTACAGCAA GTAGTATGTT
     2941 CAATTAGCTT TTCAATTATG AATATATATA TCAATTATTG GTCGCCCTTG GCTTGTGGAC
     3001 AATGCGCTAC GCGCACCGGC TCCGCCCGTG GACAACCGCA AGCGGTTGCC CACCGTCGAG
     3061 CGCCAGCGCC TTTGCCCACA ACCCGGCGGC CGGCCGCAAC AGATCGTTTT ATAAATTTTT
     3121 TTTTTTGAAA AAGAAAAAGC CCGAAAGGCG GCAACCTCTC GGGCTTCTGG ATTTCCGATC
     3181 CCCGGAATTA GAGATCTTGG CAGGATATAT TGTGGTGTAA CGTTATCAGC TTGCATGCCG
     3241 GTCGATCTAG TAACATAGAT GACACCGCGC GCGATAATTT ATCCTAGTTT GCGCGCTATA
     3301 TTTTGTTTTC TATCGCGTAT TAAATGTATA ATTGCGGGAC TCTAATCATA AAAACCCATC
     3361 TCATAAATAA CGTCATGCAT TACATGTTAA TTATTACATG CTTAACGTAA TTCAACAGAA
     3421 ATTATATGAT AATCATCGCA AGACCGGCAA CAGGATTCAA TCTTAAGAAA CTTTATTGCC
     3481 AAATGTTTGA ACGATCTGCT TGACTCTAGG GGTCATCAGA TTTCGGTGAC GGGCAGGACC
     3541 GGACGGGGCG GCACCGGCAG GCTGAAGTCC AGCTGCCAGA AACCCACGTC ATGCCAGTTC
     3601 CCGTGCTTGA AGCCGGCCGC CCGCAGCATG CCGCGGGGGG CATATCCGAG CGCCTCGTGC
     3661 ATGCGCACGC TCGGGTCGTT GGGCAGCCCG ATGACAGCGA CCACGCTCTT GAAGCCCTGT
     3721 GCCTCCAGGG ACTTCAGCAG GTGGGTGTAG AGCGTGGAGC CCAGTCCCGT CCGCTGGTGG
     3781 CGTGGAGATA CGTACACGGT GGACTCGGCC GTCCAGTCGT AGGCGTTGCG TGCCTTCCAG
     3841 GGACCCGCGT AGGCGATGCC GGCGACCTCG CCGTCCACCT CGGCGACGAG CCAGGGATAG
     3901 CGCTCCCGCA GACGGACGAG GTCGTCCGTC CACTCCTGCG GTTCCTGCGG CTCGGTACGG
     3961 AAGTTGACCG TGCTTGTCTC GATGTAGTGG TTGACGATGG TGCAGACCGC CGGCATGTCC
     4021 GCCTCGGTGG CACGGCGGAT GTCGGCCGGG CGTCGTTCTG GGCTCATGGT AGATCCCCTC
     4081 GATCGAGTTG AGAGTGAATA TGAGACTCTA ATTGGATACC GAGGGGAATT TATGGAACGT
     4141 CAGTGGAGCA TTTTTGACAA GAAATATTTG CTAGCTTATA GTGACCTTAG GCGACTTTTG
     4201 AACGCGCAAT AATGGTTTCT GACGTATGTG CTTAGCTCAT TAAACTCCAG AAACCCGCGG
     4261 CTCAGTGGCT CCTTCAACGT TGCGGTTCTG TCAGTTCCAA ACGTAAAACG GCTTGTCCCG
     4321 CGTCATCGGC GGGGGTCATA ACGTGACTCC CTTAATTCTC CGCTCATGAT CGATAACATT
     4381 AACGCTTACA ATTTCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG
     4441 CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT
     4501 TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTT
//