LOCUS       pPSt_35Spr-GFP-t35S 5672 bp ds-DNA     circular     21-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..17
                     /label="M13 fwd"
     promoter        27..45
                     /label="T7 promoter"
     primer_bind     71..87
                     /label="KS primer"
     misc_feature    117..286
                     /label="35S enhancer (optimized for Golden Gate)"
     misc_feature    291..341
                     /label="35S min promoter (-46 - +5)"
     misc_feature    342..409
                     /label="Maize 5'-UTR (new TSS annotation)"
     primer          380..412
                     /label="TJ1237"
                     /note="sequence: CCTCGGCCTCCCTGTCCCCAGCCTTCCCCGATG"
     primer          complement(380..412)
                     /label="TJ1240"
                     /note="sequence: CATCGGGGAAGGCTGGGGACAGGGAGGCCGAGG"
     misc_feature    410..412
                     /label="start codon"
     misc_feature    413..430
                     /label="barcode"
     primer          431..463
                     /label="TJ1168"
                     /note="sequence: GTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTG"
     primer          complement(431..463)
                     /label="TJ1167"
                     /note="sequence: CACCCCGGTGAACAGCTCCTCGCCCTTGCTCAC"
     CDS             431..1147
                     /label="EGFP"
     primer          1145..1167
                     /label="SG0012"
                     /note="sequence: GGACGACGTCGTCTCCTAAGCTCTAGCTAGAGTCGATCG"
     misc_feature    1149..1352
                     /label="35S_terminator"
     primer          complement(1328..1356)
                     /label="SG0013"
                     /note="sequence: GTACGACGTCGTCTCGACCTATCTGGATTTTAGTACTGGATTTTG"
     promoter        complement(1421..1439)
                     /label="T3 promoter"
     primer_bind     complement(1460..1476)
                     /label="M13 rev"
     protein_bind    1484..1500
                     /label="lac operator"
     promoter        complement(1508..1538)
                     /label="lac promoter"
     protein_bind    1553..1574
                     /label="CAP binding site"
     misc_feature    1813..1837
                     /label="RB T-DNA repeat"
     rep_origin      complement(1928..2516)
                     /label="ori"
     CDS             complement(2687..3475)
                     /label="SmR"
     rep_origin      3766..4201
                     /label="pSa ori"
     misc_feature    4336..4358
                     /label="LB T-DNA repeat"
     terminator      4382..4634
                     /label="NOS terminator"
     CDS             complement(4654..5205)
                     /label="BlpR"
     promoter        complement(5247..5426)
                     /label="NOS promoter"
ORIGIN
        1 GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG
       61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCGC TGACTCGGAA
      121 AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGGTGGACCC CCACCCACGA
      181 GGAGCATCGT GGAAAACGAA GTCGTTCCAA CCACGGCTTC AAAGCAAGTG GATGGATGTG
      241 ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCCTGT GCAAGACCCT
      301 TCCTCTATAT AAGGAAGTTC ATTTCATTTG GAGAGGACAC GCCCGTCCGA ACTCCGAACC
      361 CCAGAACAGA GCAAAGCCTC CTCGGCCTCC CTGTCCCCAG CCTTCCCCGA TGANNANNGN
      421 NGNNANNGNN GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
      481 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
      541 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
      601 GCCCACCCTC GTGACCACCT TCACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
      661 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
      721 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
      781 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
      841 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
      901 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
      961 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
     1021 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
     1081 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CACGGCATGG ACGAGCTGTA
     1141 CAAGTAAGCT CTAGCTAGAG TCGATCGACA AGCTCGAGTT TCTCCATAAT AATGTGTGAG
     1201 TAGTTCCCAG ATAAGGGAAT TAGGGTTCCT ATAGGGTTTC GCTCATGTGT TGAGCATATA
     1261 AGAAACCCTT AGTATGTATT TGTATTTGTA AAATACTTCT ATCAATAAAA TTTCTAATTC
     1321 CTAAAACCAA AATCCAGTAC TAAAATCCAG ATAGGTGTTC CACTTCTGTA TAGGAGCCAA
     1381 TGATGACAAC ATGCATCAAT CGGAGCTCCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTG
     1441 CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
     1501 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
     1561 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
     1621 GCCAGCTGCA TTAATGAATC GGCCAACGCT CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT
     1681 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
     1741 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
     1801 ACATGAAGGC CTTGACAGGA TATATTGGCG GGTAAACTAA GTCGCTGTAT GTGTTTGTTT
     1861 GAGATCTCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
     1921 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     1981 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     2041 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     2101 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     2161 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     2221 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     2281 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     2341 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     2401 CAGTTACCTT CGGAAGAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     2461 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     2521 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
     2581 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
     2641 GTTTTAAATC AATCTAAAGT ATATATGTGT AACATTGGTC TAGTGATTAT TTGCCGACTA
     2701 CCTTGGTGAT CTCGCCTTTC ACGTAGTGAA CAAATTCTTC CAACTGATCT GCGCGCGAGG
     2761 CCAAGCGATC TTCTTGTCCA AGATAAGCCT GCCTAGCTTC AAGTATGACG GGCTGATACT
     2821 GGGCCGGCAG GCGCTCCATT GCCCAGTCGG CAGCGACATC CTTCGGCGCG ATTTTGCCGG
     2881 TTACTGCGCT GTACCAAATG CGGGACAACG TAAGCACTAC ATTTCGCTCA TCGCCAGCCC
     2941 AGTCGGGCGG CGAGTTCCAT AGCGTTAAGG TTTCATTTAG CGCCTCAAAT AGATCCTGTT
     3001 CAGGAACCGG ATCAAAGAGT TCCTCCGCCG CTGGACCTAC CAAGGCAACG CTATGTTCTC
     3061 TTGCTTTTGT CAGCAAGATA GCCAGATCAA TGTCGATCGT GGCTGGCTCG AAGATACCTG
     3121 CAAGAATGTC ATTGCGCTGC CATTCTCCAA ATTGCAGTTC GCGCTTAGCT GGATAACGCC
     3181 ACGGAATGAT GTCGTCGTGC ACAACAATGG TGACTTCTAC AGCGCGGAGA ATCTCGCTCT
     3241 CTCCAGGGGA AGCCGAAGTT TCCAAAAGGT CGTTGATCAA AGCTCGCCGC GTTGTTTCAT
     3301 CAAGCCTTAC GGTCACCGTA ACCAGCAAAT CAATATCACT GTGTGGCTTC AGGCCGCCAT
     3361 CCACTGCGGA GCCGTACAAA TGTACGGCCA GCAACGTCGG TTCGAGATGG CGCTCGATGA
     3421 CGCCAACTAC CTCTGATAGT TGAGTCGATA CTTCGGCGAT CACCGCTTCC CTCATAACAC
     3481 CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGATGAT ATATTTTTAT
     3541 CTTGTGCAAT GTAACATCAG AGATTTTGAG ACACAACGTG GCTTTGTTGA ATAAATCGAA
     3601 CTTTTGCTGA GTTGAAGGAT CAGATCACGC ATCTTCCCGA CAACGCAGAC CGTTCCGTGG
     3661 CAAAGCAAAA GTTCAAAATC ACCAACTGGT CCACCTACAA CAAAGCTCTC ATCAACCGTG
     3721 GCTCCCTCAC TTTCTGGCTG GATGATGGGG CGATTCAGGC GATCCCCATC CAACAGCCCG
     3781 CCGTCGAGCG GGCTTTTTTA TCCCCGGAAG CCTGTGGATA GAGGGTAGTT ATCCACGTGA
     3841 AACCGCTAAT GCCCCGCAAA GCCTTGATTC ACGGGGCTTT CCGGCCCGCT CCAAAAACTA
     3901 TCCACGTGAA ATCGCTAATC AGGGTACGTG AAATCGCTAA TCGGAGTACG TGAAATCGCT
     3961 AATAAGGTCA CGTGAAATCG CTAATCAAAA AGGCACGTGA GAACGCTAAT AGCCCTTTCA
     4021 GATCAACAGC TTGCAAACAC CCCTCGCTCC GGCAAGTAGT TACAGCAAGT AGTATGTTCA
     4081 ATTAGCTTTT CAATTATGAA TATATATATC AATTATTGGT CGCCCTTGGC TTGTGGACAA
     4141 TGCGCTACGC GCACCGGCTC CGCCCGTGGA CAACCGCAAG CGGTTGCCCA CCGTCGAGCG
     4201 CCAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT AAATTTTTTT
     4261 TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT TTCCGATCCC
     4321 CGGAATTAGA GATCTTGGCA GGATATATTG TGGTGTAACG TTATCAGCTT GCATGCCGGT
     4381 CGATCTAGTA ACATAGATGA CACCGCGCGC GATAATTTAT CCTAGTTTGC GCGCTATATT
     4441 TTGTTTTCTA TCGCGTATTA AATGTATAAT TGCGGGACTC TAATCATAAA AACCCATCTC
     4501 ATAAATAACG TCATGCATTA CATGTTAATT ATTACATGCT TAACGTAATT CAACAGAAAT
     4561 TATATGATAA TCATCGCAAG ACCGGCAACA GGATTCAATC TTAAGAAACT TTATTGCCAA
     4621 ATGTTTGAAC GATCTGCTTG ACTCTAGGGG TCATCAGATT TCGGTGACGG GCAGGACCGG
     4681 ACGGGGCGGC ACCGGCAGGC TGAAGTCCAG CTGCCAGAAA CCCACGTCAT GCCAGTTCCC
     4741 GTGCTTGAAG CCGGCCGCCC GCAGCATGCC GCGGGGGGCA TATCCGAGCG CCTCGTGCAT
     4801 GCGCACGCTC GGGTCGTTGG GCAGCCCGAT GACAGCGACC ACGCTCTTGA AGCCCTGTGC
     4861 CTCCAGGGAC TTCAGCAGGT GGGTGTAGAG CGTGGAGCCC AGTCCCGTCC GCTGGTGGCG
     4921 TGGAGATACG TACACGGTGG ACTCGGCCGT CCAGTCGTAG GCGTTGCGTG CCTTCCAGGG
     4981 ACCCGCGTAG GCGATGCCGG CGACCTCGCC GTCCACCTCG GCGACGAGCC AGGGATAGCG
     5041 CTCCCGCAGA CGGACGAGGT CGTCCGTCCA CTCCTGCGGT TCCTGCGGCT CGGTACGGAA
     5101 GTTGACCGTG CTTGTCTCGA TGTAGTGGTT GACGATGGTG CAGACCGCCG GCATGTCCGC
     5161 CTCGGTGGCA CGGCGGATGT CGGCCGGGCG TCGTTCTGGG CTCATGGTAG ATCCCCTCGA
     5221 TCGAGTTGAG AGTGAATATG AGACTCTAAT TGGATACCGA GGGGAATTTA TGGAACGTCA
     5281 GTGGAGCATT TTTGACAAGA AATATTTGCT AGCTTATAGT GACCTTAGGC GACTTTTGAA
     5341 CGCGCAATAA TGGTTTCTGA CGTATGTGCT TAGCTCATTA AACTCCAGAA ACCCGCGGCT
     5401 CAGTGGCTCC TTCAACGTTG CGGTTCTGTC AGTTCCAAAC GTAAAACGGC TTGTCCCGCG
     5461 TCATCGGCGG GGGTCATAAC GTGACTCCCT TAATTCTCCG CTCATGATCG ATAACATTAA
     5521 CGCTTACAAT TTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG
     5581 GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG
     5641 GGTAACGCCA GGGTTTTCCC AGTCACGACG TT
//