LOCUS       pUC57-NHa08_H-eg        5126 bp DNA     circular SYN 18-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Adamson
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1220..1242
                     /chromosome="5"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     source          2023..2049
                     /chromosome="5"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     CDS             complement(146..412)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="VPSSNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
                     RPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     390..431
                     /label=Fragment 1.FOR
     misc_feature    414..1219
                     /label=5'h
     primer_bind     870..887
                     /label=Seq_1
     gene            1005..1250
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /label=NKX2-5
                     /note="NK2 homeobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1005..1250
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant X1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 ESTs, 1 Protein, and 100% 
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 9 samples with support for all 
                     annotated introns
                     /transcript_id=XM_017009071.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1120..1250
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_004387.4"
                     /db_xref="Ensembl:ENST00000329198.5"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1120..1250
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 3"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166176.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1120..1250
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 2"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166175.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     primer_bind     complement(1199..1252)
                     /label=Fragment 1 REV
     primer_bind     1211..1260
                     /label=Fragment 2.FOR
     misc_feature    1214..1216
                     /label=MUTATED PAM
     misc_feature    1217..1236
                     /label=g550 (mutated PAM)
     mRNA            1220..1242
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant X1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 ESTs, 1 Protein, and 100% 
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 9 samples with support for all 
                     annotated introns
                     /transcript_id=XM_017009071.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1220..1242
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 2"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166175.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1220..1242
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 3"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166176.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            1220..1242
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_004387.4"
                     /db_xref="Ensembl:ENST00000329198.5"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     gene            1220..1242
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /label=NKX2-5
                     /note="NK2 homeobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     CDS             1243..1959
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1246..1959
                     /label=GFP
                     /note="GFP"
     primer_bind     1307..1323
                     /label=Seq_2
     primer_bind     complement(1307..1323)
                     /label=Seq2_R
     primer_bind     1782..1798
                     /label=Seq_3
     primer_bind     complement(1939..2040)
                     /label=Frag 2 Rev NEW
     misc_feature    1960..2022
                     /label=T2A
                     /note="T2A"
     CDS             1969..2022
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     2014..2080
                     /label=Fragment 3 F JAN21
     gene            2023..2049
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /label=NKX2-5
                     /note="NK2 homeobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            2023..2049
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_004387.4"
                     /db_xref="Ensembl:ENST00000329198.5"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            2023..2049
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 3"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166176.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            2023..2049
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 2"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166175.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     CDS             2023..2049
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform 1"
                     /label=NKX2-5
                     /note="isoform 1 is encoded by transcript variant 1;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS4387.1"
                     /db_xref="Ensembl:ENSP00000327758.4"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="NP_004378.1"
                     /translation="MFPSPALTP"
     CDS             2023..2049
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform 3"
                     /label=NKX2-5
                     /note="isoform 3 is encoded by transcript variant 3;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS54949.1"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="NP_001159648.1"
                     /translation="MFPSPALTP"
     CDS             2023..2049
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform 2"
                     /label=NKX2-5
                     /note="isoform 2 is encoded by transcript variant 2;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS54950.1"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="NP_001159647.1"
                     /translation="MFPSPALTP"
     mRNA            2023..2049
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant X1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 ESTs, 1 Protein, and 100% 
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 9 samples with support for all 
                     annotated introns
                     /transcript_id=XM_017009071.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     CDS             2023..2049
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform X1"
                     /label=NKX2-5
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="XP_016864560.1"
                     /translation="MFPSPALTP"
     misc_feature    2050..2069
                     /label=Mutated g542
     misc_feature    2053..2850
                     /label=3'H
     gene            2053..2850
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /label=NKX2-5
                     /note="NK2 homeobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            2053..2356
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_004387.4"
                     /db_xref="Ensembl:ENST00000329198.5"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            2053..2356
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 3"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166176.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     mRNA            2053..2356
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant 2"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_001166175.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     CDS             2053..2356
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform 1"
                     /label=NKX2-5
                     /note="isoform 1 is encoded by transcript variant 1;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS4387.1"
                     /db_xref="Ensembl:ENSP00000327758.4"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="NP_004378.1"
                     /translation="PFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEA
                     YAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKK"
     CDS             2053..2356
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform 3"
                     /label=NKX2-5
                     /note="isoform 3 is encoded by transcript variant 3;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS54949.1"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="NP_001159648.1"
                     /translation="PFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEA
                     YAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKK"
     CDS             2053..2356
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform 2"
                     /label=NKX2-5
                     /note="isoform 2 is encoded by transcript variant 2;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS54950.1"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="NP_001159647.1"
                     /translation="PFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEA
                     YAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKK"
     mRNA            2053..2356
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="NK2 homeobox 5, transcript variant X1"
                     /label=NKX2-5 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 ESTs, 1 Protein, and 100% 
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 9 samples with support for all 
                     annotated introns
                     /transcript_id=XM_017009071.2"
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
     CDS             2053..2356
                     /codon_start=1
                     /gene="NKX2-5"
                     /gene_synonym="CHNG5; CSX; CSX1; HLHS2; NKX2.5; 
                     NKX2E;NKX4-1; VSD3"
                     /product="homeobox protein Nkx-2.5 isoform X1"
                     /label=NKX2-5
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:1482"
                     /db_xref="HGNC:HGNC:2488"
                     /db_xref="MIM:600584"
                     /protein_id="XP_016864560.1"
                     /translation="PFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEA
                     YAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKK"
     misc_feature    2085
                     /label=SNP in MAN13?
     primer_bind     2253..2269
                     /label=Seq_4
     primer_bind     complement(2836..2870)
                     /label=Fragment 3.REV
     CDS             complement(2856..2909)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHAGLCSRRAR"
     primer_bind     complement(2905..2921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2929..2945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2953..2983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2998..3019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3307..3895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4066..4926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4927..5031)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctggcagt
      421 gactgatgcc ccctggagtt gtgctgtgca taacactact gtaggaggca gcaactccta
      481 ccccacctgg ccatcactca ccttgccctt actttcgttg actcgcccag aagcacccag
      541 agcctgcggc atgactgacc ctgtaggcca agccaaacca aacccccgaa ttgtccagaa
      601 tctcgccctg ctgctatccc caaagccagc cctgtcctcc aggcttctct tcctattcag
      661 actctccctc atcccaccac cttcagtaat aaagccttcc tcaaatcact ctcctcccca
      721 ccgcttccca ccccatcctt ctctctccca tgctggtttg ggtgctgagg aatatttctt
      781 caaacccaca cccatccagc cctgcccaga ggcctgactc tgcatgcctc tggtaggctt
      841 ttcaggctca cattagggag caaacgcagg ctgcaggagc aaaaggagac ccttccaaat
      901 gcgtcgtggc ccctttaaaa aagctgggca gggctttttt tttttttttt tttttttttt
      961 tttttttttt tttttgccgt atgactatat taggtgacac gaaactgctc atcgctcctg
     1021 tcatcgaggc ccctggccca atggcaggct gagtccccct cctctggcct ggtcccgcct
     1081 ctcctgcccc ttgtgctcag cgctacctgc tgcccggaca catccagagc tggccgacgg
     1141 gtgcgcgggc gggcggcggc accatgcagg gaagctgcca ggggccgtgg gcagcgccgc
     1201 tttctgccgc ccatctggcg ctgtgagact ggcgctgcca ccatggtgag caagggcgag
     1261 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1321 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1381 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1441 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1501 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1561 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1621 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1681 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1741 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1801 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1861 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1921 gccgccggga tcactctcgg catggacgag ctgtacaagg gcagtggaga gggcagagga
     1981 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgttccc cagccctgct
     2041 ctcacgccca ctccgttctc agtcaaagac atcctaaacc tggagcagca gcagcgcagc
     2101 ctggctgccg ccggagagct ctctgcccgc ctggaggcga ccctggcgcc ctcctcctgc
     2161 atgctggccg ccttcaagcc agaggcctac gctgggcccg aggcggctgc gccgggcctc
     2221 ccagagctgc gcgcagagct gggccgcgcg ccttcaccgg ccaagtgtgc gtctgccttt
     2281 cccgccgccc ccgccttcta tccacgtgcc tacagcgacc ccgacccagc caaggaccct
     2341 agagccgaaa agaaaggtga ggaggaaaca caggccccct tctcccctcc tgggtcgctt
     2401 tcgtccccaa gaaactcagg gccaggagga ggagacacgc gcccttgggc cgagggctgg
     2461 gctgcggcgg ggggttcaga atgtaagatg cctggtgttg tcgccaggct cccgcgcccc
     2521 gcgtccaatc ggaggttcag aggaaatgcc ggattgaaag gatcagaagc aagagaccaa
     2581 aaaacgtttc cccccggcct aacaaagccc cgggcggctt cggctctgct cctgggtctg
     2641 gtaggaagtt gagaaatcgg tttatggtag acagaacaga gagacaagca gataatctct
     2701 gtttttaaat ctcctttgga tttacgaatc tttttaaaga tctgatgaga accgctaaac
     2761 agaaattgaa atgttgctca ccagacagct tttgcgtaca atcggaggag ggtcctggac
     2821 cttctttctg cagcccaccc acgacccggg ggatcccggg cccgtcgact gcagaggcct
     2881 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2941 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3001 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3061 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3121 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3241 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3301 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3361 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3421 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3481 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3541 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3601 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3661 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3721 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3781 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3841 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3901 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3961 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4021 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4081 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4141 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4201 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4261 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4321 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4381 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4441 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4501 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4561 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4621 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4681 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4741 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4801 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4861 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4921 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4981 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5041 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5101 taggcgtatc acgaggccct ttcgtc
//
