LOCUS       FB372__seq_CVoxx        8818 bp ds-DNA     circular     01-SEP-2023
DEFINITION  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2450..2453
                     /locus_tag="GTCA"
                     /label="GTCA"
                     /ApEinfo_label="GTCA"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2466..2490
                     /locus_tag="RB"
                     /label="RB"
                     /ApEinfo_label="RB"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3552..4552)
                     /locus_tag="STA\region\from\pVS1\plasmid"
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5145..6145)
                     /locus_tag="pVS1-Rep\ori"
                     /label="pVS1-Rep\ori"
                     /ApEinfo_label="pVS1-Rep\ori"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6555..6815)
                     /locus_tag="pBR322"
                     /label="pBR322"
                     /ApEinfo_label="pBR322"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6955..7235)
                     /locus_tag="pBR322\ori"
                     /label="pBR322\ori"
                     /ApEinfo_label="pBR322\ori"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(7530..8342)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8775..8800
                     /locus_tag="LB"
                     /label="LB"
                     /ApEinfo_label="LB"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1207..2417
                     /locus_tag="3' downstream"
                     /label="3' downstream"
                     /ApEinfo_label="3' downstream"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3..1206
                     /locus_tag="5' upstream"
                     /label="5' upstream"
                     /ApEinfo_label="5' upstream"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 agGATGAAGG ATCCAGAAAA TCCTTCAATC TGAAGTCATC CTCAATGCTC CGAAAGGCCA
       61 ACTATATCAC AACAGTAGAC AAGAAAACCT TGGAAGACCC CGGATCGGCT AAATCGATTG
      121 CACCGACGGG ACGTCGGAAC CTGTGAGCCC CGAACCTCCA AATATGTATA AAGGAGCCCA
      181 GGCATCAGGC TAAATCACAA CACAAAGCCG CTCTCTCACT TCATTTCCCC GGTTAATCTT
      241 CTCATAATGG TCGCATTCAT TGTTCCTGGC AACTACGGGT AGGTTATGCC ACTCCCGGCT
      301 TAACCTTCGC GTTTCCTGTG AAATCCGGGG CTAACATAAC TTCCCAGAGC CGTCATTGCC
      361 GTGGCCCTCG GTGCCATCCC TGTTTTGGGC TTTATTCACG GCAATGTCAC TGGTAGCCTG
      421 CGCAAGGCGG CCAAGGTGCC CTACCCCCAC AGCTATGCCT CCATGGAGCT ATGCAAGGAA
      481 AACGTGCGTA GAAATTTTTT TTCCATCTGT CGGCCTTCAA AAGCCCTTCA GTTCAAGGGA
      541 AACATGTGTA ATCTCTAATG AACACCCCAT TAAACCTGCT AATCTGATTA TAGGCAAAAG
      601 CTGAGCAGTT CAACTGCGCC CAGCGCGCCC ATGCCAACTT CCTCGAGAAC GCCTCGCAGA
      661 CAATGCTCTT TACCTTGGTC GCCGGTCTAA AGTATCCGGA GTGGGCCGCT GGTCTGGGCG
      721 CTCTCTGGGT TTTCTTCCGC GTTCTCTTCC TCTACGGCTA CGTTTACTCG GGCAAGCCGC
      781 AGGGTAAGGG CCGCTTCATG GGCGGGTTCT TCTGGCTTGT GCAGGGTGCT CTTTGGGGAT
      841 TGAGCATCTA CGGTGTTGGA AGGCCTCTGC TTTCTTTCTA AGAGCTTGAA GCCTGGGGAT
      901 CCTCTAAAAT GTCTTGAAAT TGAAGATGTA CTCTGTATTT ATAAGTCCAA TGCAAGCAAC
      961 GTATTAGTTA TGCGCCACAG TTCCAGCCTC GGAAGGTTAT GTTGTAGGCC TGCGGGGCCC
     1021 ACCGTGGCAG AAAAAGTTCC CCGATGGTCT TCAAATCGAA CTTGCTCCTC ATCTTCTGCT
     1081 TTTTGGACCC CAAAAAGCCT ATAGTTCTCT GAAGGCATTT GTCGTTTGAT TCATTTCCAT
     1141 CCTTTCGACC CCTCAAAACA CAAGCTAAAT ACACAACAAT CGCCTCTTCA CCCCGCCAGC
     1201 CCCGCCAATG CCAGCCCGGT ACTTTCTTGC CTCAGATTAT TAGTGAGTCT TTCCAGGGCT
     1261 CTAAAAGGGC TTCCCTGCAT GTGTAAACGA TGCAAGGGCA ATTTCAAGTC TTACGTGTGC
     1321 TCGACGATAT GGTTCACTCC CCCTTCATTG AATCATCAAA TCAAACGCGA ATCTCTGAAT
     1381 ATTCGAATGT TGGGAGTAGC TTGATAAGGC GGCTCCAAAC TTCAGATGTC GAGATACCGG
     1441 ACAACAGATC AGAGTAATAC TGTAATTATG TGTCAACCTC ATCAGAACCA GCAGAACTAG
     1501 AAGCGTAAGC ATGTCGACAT ATAGACCGGA TAATGCCTGG CACTCGAGAT CTTTCCCTTT
     1561 CCGTTTTAGT GCTCACCCCT TGCCGCTTTT TGATCACTGA GTAAAACCGA GCACGTCCCT
     1621 GAGTATCCTG GCTTGATCAC AGGTCATTAC ATAATCAACT GATCTGCTCA CTGAGCATCA
     1681 TCTGAGCACT CTGATTTGAT CACTGAGTGC TCACTGAGTG TAGGGATGAG CGGGCTGAGT
     1741 AAGCTACATC AATAAAATGC GACACGCTAA TCCCATCACG TGCAACCATT CCCATTCAGA
     1801 TAGCCCATCA AAAACGGACT GGCACAATCA ATGTGCCCGT TCGTTCTAGC GGCCTCGATT
     1861 CTATCCAAGT AAAAAAGCCC CACAGTGGGG CTATTAATTG ACCTACCATC TTCTCATTTC
     1921 AGCCTATGCT CCTGGGAATA CCTCACGGAA TACCCTAATC GGGCTTTGGG CTACTACCAG
     1981 GGCGAGGGCG ATTGAATGAG TTTCCTTGAA GATCTAGAAT CATTCCCTGT GTCGGCAACA
     2041 GGAGAAATTA GTGTTCAGTA AGTGACGATG ATGAGCCACT TCTGTCGCCA ATGAGCGCAC
     2101 GACCCAAAGG ATCTCCTGGG CAGATAACGT CATGTCTTTC ACGCCATTGC TAAGTAATCT
     2161 GTAAGCCGAA GACAAACCAT CAATAATAAT ACCCGATGAG CTCCTGGGAG CCTGCCATGC
     2221 ATTCCCATGA GCCCACTAAG CTGTATTACC AGTGTCATAG GGGCCCATAG CTATTTCGTT
     2281 TGAATCCTAG GAACTCTAAA CATTGCCCCC CACCCCTAGC CTGTCTGATT TCTGCCTAAA
     2341 GCGATGCCAT TCCAAAAGGG ATATGTATCT AGCCACTTGC TCGGAAACAA TGGTCTTCAT
     2401 CATTCCTCTG CCTGCTTgct tttgctaacc atttgggaca ccgctcgctg tcatgagacg
     2461 aattctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     2521 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     2581 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     2641 gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa
     2701 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     2761 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     2821 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     2881 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     2941 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3001 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     3061 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     3121 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3181 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     3241 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     3301 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     3361 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     3421 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     3481 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     3541 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg
     3601 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     3661 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     3721 acagcttgcg tcatgcggtc gctgcgtata tgatccgatg agtaaataaa caaatacgca
     3781 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     3841 catcggaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     3901 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     3961 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4021 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     4081 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     4141 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4201 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     4261 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     4321 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     4381 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     4441 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     4501 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     4561 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     4621 ctgctatctg aatagatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     4681 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     4741 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     4801 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     4861 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     4921 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     4981 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     5041 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     5101 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     5161 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     5221 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     5281 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     5341 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     5401 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     5461 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     5521 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     5581 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     5641 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     5701 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     5761 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     5821 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     5881 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     5941 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt
     6001 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     6061 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     6121 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     6181 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     6241 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     6301 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     6361 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6421 tgacacatgc agctcccggt gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6481 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     6541 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     6601 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     6661 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7261 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7501 ggattttggt catgcattct aggtgattag aaaaactcat cgagcatcaa atgaaactgc
     7561 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     7621 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     7681 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     7741 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     7801 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     7861 accaaaccgt tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta
     7921 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     7981 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg
     8041 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     8101 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     8161 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcgg
     8221 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     8281 gcatccatgt tggaatttaa tcgcggcctt gagcaagacg tttcccgttg aatatggctc
     8341 ataacagaac ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     8401 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
     8461 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     8521 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     8581 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     8641 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     8701 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     8761 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca taacgaattc gtctcagg
//
