LOCUS       FB431                   8942 bp ds-DNA     linear       30-MAY-2023
DEFINITION  (GB_UD_2B0D,pUPD2_ergA_(CDS)_(FB414,GB_UD_FB3E)pDGB3_alpha2.
ACCESSION   GB_UA_8FF2
VERSION     GB_UA_8FF2
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5..732
                     /locus_tag="PpcbC"
                     /label="PpcbC"
                     /ApEinfo_label="PpcbC"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    737..2213
                     /locus_tag="ergA_Pc22g15550"
                     /label="ergA_Pc22g15550"
                     /ApEinfo_label="ergA_Pc22g15550"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1450..1450
                     /locus_tag="G-->A for BsaI-site removal"
                     /label="G-->A for BsaI-site removal"
                     /ApEinfo_label="G-->A for BsaI-site removal"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     domest_feature  2218..2569
                     /locus_tag="TamdS"
                     /label="TamdS"
                     /ApEinfo_label="TamdS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2587..2611
                     /vntifkey="21"
                     /locus_tag="RB"
                     /label="RB"
                     /ApEinfo_label="RB"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8896..8921
                     /vntifkey="21"
                     /locus_tag="LB"
                     /label="LB"
                     /ApEinfo_label="LB"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(7651..8463)
                     /vntifkey="4"
                     /note="kanamycin resistance gene"
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(7076..7356)
                     /vntifkey="33"
                     /note="pBR322 origin of replication"
                     /locus_tag="pBR322\ori"
                     /label="pBR322\ori"
                     /ApEinfo_label="pBR322\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6676..6936)
                     /vntifkey="21"
                     /note="bom site from pBR322"
                     /locus_tag="pBR322"
                     /label="pBR322"
                     /ApEinfo_label="pBR322"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3673..4673)
                     /vntifkey="21"
                     /note="STA region from pVS1 plasmid"
                     /locus_tag="STA\region\from\pVS1\plasmid"
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5266..6266)
                     /vntifkey="21"
                     /note="pVS1-REP; replication origin from pVS1"
                     /locus_tag="pVS1-Rep\ori"
                     /label="pVS1-Rep\ori"
                     /ApEinfo_label="pVS1-Rep\ori"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggagtggcgc atgcggacag acggacggac gcagagagaa gggctgagta ataagcgcca
       61 ctgcgccaga cagctctggc ggctctgagg tgcagtggat gattattaat ccgggaccgg
      121 ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa gaatctattg
      181 catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg agaatgtgaa
      241 gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac agaagtccaa
      301 ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta ggtagagcga
      361 gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg tccaaatatc
      421 gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga gctggccagc
      481 ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca ctcgacctgc
      541 tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg tgtgtcggcg
      601 gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct gctctcccca
      661 ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat cttcccatcc
      721 aagaaccttt aaaatgacct tgctcaatgg ccacgcctcc ctctccccgg ccgctcaacg
      781 ccgcatcgaa caccatgaag ctgatattgt aattgtgggt gccggagtcc tcggctgtgc
      841 acttgcagtt gcactgggaa atcaagggcg cagtgtaatc ctgctggaga agtcgttgga
      901 agagcccaac cgtattgttg gtgaactact ccaaccggga ggtgttcagg cgctcgagca
      961 actagggcta cgggattgtc tggaagatat cgatggcatc aatgtgaagg gatactgggt
     1021 tacttacttt ggggagccgg ttctcctcga ataccccaag tccagcccaa cctcgcctac
     1081 accgttggga cgagccttcc accatggccg attcgtgatg aaactacggg cagccgctct
     1141 gtcgtgcccc aatgtcacgg tggtcgaaac caaggttacc ggcctcatca catcctcata
     1201 tactcaggaa gtccttggag tcgagtgtat aaccaaggat gtcaaggact gctacttcgg
     1261 tcaactcacc gtggccgccg acggttataa ctccgttttc cgcaaggaac accaccagta
     1321 tacacccaaa cggcgctcaa agttccatgg cttggagctc atcgatgcta aactgcccgc
     1381 tcccaacact ggtcatgtgc ttctcagcga taatccaccc gtacttatgt accagatcgg
     1441 cacccatgaa acccgaatcc ttatcgatat ccctgataac ctgcctgcgg cctcggtcaa
     1501 gaacggtggt gtcaagggtt acatgcgaaa caacatcctg cccaagctcc cagagggtgt
     1561 ccagcagtcg ttctccgatg ccctagagaa gggccagcta cggtcaatgc cgaactcgtt
     1621 cctgcctgcc tcggccaaca agacacccgg cttgatgatt ctgggcgatg cgcttaacat
     1681 gcggcaccct ctcacaggtg gaggcatgac tgtggcgctc aacgatgttt gtgtcatccg
     1741 cgaattgctc agcccagagc gcgtgcccaa cctctccaat acaggccttg ttctcgagca
     1801 gctcgcagag ttccactgga aacgaaagaa ctcatcttcg gtcatcaata tcctcgcaca
     1861 ggctctctat gcgctcttcg ctgcagacag tacgcacgct cactcttcag gttaatttgc
     1921 aataactaac tgttcattca acagactatt acctcaaagc tcttcagcgt ggctgtttcc
     1981 gctacttcca gattggacca gttggcggcc ctgtcgggtt gttagccggt ctaatcaaga
     2041 aacccctcgt ccttgtcagc cacttcttct ccgttgcctt cctcgccatc tgggtccaca
     2101 tatgcgatac ccctctgtcc aagctattcc tagccccata ctacgccgtc atgatattgt
     2161 acacagcctc tgtcgtgatt ttgccataca tctggaccga gatctggtac tgagcttcta
     2221 ataagtgtca gatagcaatt tgcacaagaa atcaatacca gcaactgtaa ataagcgctg
     2281 aagtgaccat gccatgctac gaaagagcag aaaaaaacct gccgtagaac cgaagagata
     2341 tgacacgctt ccatctctca aaggaagaat cccttcaggg ttgcgtttcc agtctagaca
     2401 cgtataacgg cacaagtgtc tctcaccaaa tgggttatat ctcaaatgtg atctaaggat
     2461 ggaaagccca gaatattggc tgggttgatg gctgcttcga gtgcagtctc atgctgccac
     2521 aggtgactct ggatggcccc ataccactca acccatggta cgagcggtac gcttgagacg
     2581 aagctttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     2641 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     2701 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     2761 agtcggcttc tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc acaagtccta
     2821 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     2881 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     2941 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     3001 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     3061 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     3121 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     3181 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     3241 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     3301 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     3361 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     3421 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     3481 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     3541 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     3601 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     3661 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cctctcaacc
     3721 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     3781 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     3841 aacagcttgc gtcatgcggt cgctgcgtat atgatccgat gagtaaataa acaaatacgc
     3901 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     3961 ccatcggaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     4021 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     4081 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     4141 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     4201 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     4261 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     4321 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     4381 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     4441 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     4501 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     4561 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     4621 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     4681 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     4741 gctgctatct gaatagatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     4801 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     4861 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     4921 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc
     4981 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     5041 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     5101 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     5161 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     5221 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     5281 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     5341 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     5401 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     5461 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     5521 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     5581 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     5641 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     5701 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     5761 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     5821 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     5881 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     5941 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     6001 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     6061 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg
     6121 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     6181 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     6241 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     6301 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     6361 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     6421 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     6481 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     6541 ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag
     6601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     6661 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     6721 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6781 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6961 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7381 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7441 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7501 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7561 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7621 gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg
     7681 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     7741 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     7801 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     7861 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     7921 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     7981 aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt
     8041 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     8101 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg
     8161 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     8221 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     8281 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     8341 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     8401 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct
     8461 cataacagaa cttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     8521 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
     8581 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
     8641 cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     8701 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     8761 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     8821 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     8881 ctgttggctg gctggtggca ggatatattg tggtgtaaac ataacaagct tcgtctcagt
     8941 ca     
//
