LOCUS       FB407                   2787 bp ds-DNA     circular     01-SEP-2023
DEFINITION  Promoter of the elongation factor 1-alpha gene from P. digitatum
            (PDIG_59570)..
ACCESSION   .
VERSION     .
KEYWORDS    FB407
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Marcos Lab
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the
            biotechnological exploitation of filamentous fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2023 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1902..2490
                     /locus_tag="pMB1"
                     /label="pMB1"
                     /ApEinfo_label="pMB1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..660
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_label="CamR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    931..1608
                     /locus_tag="Pef1A"
                     /label="Pef1A"
                     /ApEinfo_label="Pef1A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ttacgccccg ccctgccact cgtcgcagta ctgttgtaat tcattaagca ttctgccgac
       61 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      121 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      181 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgatacga aaaacatatt
      241 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      301 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagtg atgaaaacgt
      361 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      421 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      481 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      541 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      601 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      661 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
      721 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      781 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      841 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      901 cgcttctaga gcgatgagtg gtctcgggag GTGAAAAAAC GGATGGGGAC ATCCAATATT
      961 GCCACAGTAA TTCGTACAGG CCCAAAGAAA GCAGAAAAAA AGCACAAAGT ACGGTGTAAA
     1021 CAGTGCAAAC TGCGTTGTAG AAGTTTCTCA CCCAACCACT CTGCGTTGCT GCCCAGTTTC
     1081 AAGCGTTTAG AGCCACAAAG TGGGCTGGTA CGGAGCAGAT GAAAAAAAAA AAAATTTGCA
     1141 GAGGGTGGAG TGTCATGGCA GGACACAACC AGGGAAACGG AGGGGTGTTT CAAGAACAGA
     1201 AGTAGACAGT ACATCGTAAG GTATGGTTGC CAACATGTTG AAGCAAGTAC ATCTACCTCA
     1261 GCCGACCAAT CCACTTTGCA AAATCTGCGT ACACGGGTAA GGGCTAAAGC CAATCAGGAG
     1321 CCCTACCCAC AGAGGGCTTG GCCCCACCTG CTGACTCGCC TCGCTCGCTT GCCCCTCCAT
     1381 ATAAGGATTT CCCCTCCTGC CCTCCCACAG AGTTTTCTTT CTTCCTCTTG TCTGCTACAG
     1441 TGAGTTTTTT CTCCCCCCCC TACTGCTATT CAGCTCTCTC TTCCGACTAC TTTTGCTGAT
     1501 TACTATTGCT CAGTACCTCT ACGCGTTCTG CCGTAGTCTC TGAGCAAGTT TTTCCACAGA
     1561 TTTTTTCTAG TATCTTACAA AGAACTACAA AAAGTTACAA CCGTCATCaa tgtgagacca
     1621 ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
     1681 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
     1741 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
     1921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2641 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     2701 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2761 aggagtccaa gcgagctcga tatcaaa   
//
