LOCUS       VSVG-mCherry        7453 bp DNA     circular SYN 31-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(213..1073)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1074..1178)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1237..1289
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     enhancer        1444..1823
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1824..2027
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2023..2056
                     /label=5' LTR (truncated) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=18.8"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: RIGHT"
     promoter        2072..2090
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2163..2276
                     /codon_start=1
                     /label=STIM1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.5"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGA"
     CDS             2286..2762
                     /codon_start=1
                     /label=SAV_STRAV (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=86.9"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="DPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYES
                     AVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARI
                     NTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ"
     sig_peptide     2772..2783
                     /label=KDEL
                     /note="color: #cc99b2"
     intron          2855..3084
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
                     /note="color: #------; direction: RIGHT"
     misc_feature    3267..3729
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: RIGHT"
     sig_peptide     3756..3814
                     /label=IL-2 signal sequence
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.3"
                     /note="/other=sig_peptide"
                     /note="color: #cc99b2; direction: RIGHT"
     CDS             3816..3926
                     /label=SBP
                     /note="color: #993366; direction: RIGHT"
     CDS             3936..4640
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             4653..6140
                     /codon_start=1
                     /label=VSVG (without signal peptide)
                     /note="color: #993366"
                     /translation="KFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKM
                     PKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTW
                     LNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHN
                     STTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILDY
                     SLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAPIL
                     SRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDLHLS
                     SKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFFIIGL
                     IIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     5351..5370
                     /label=VSVG-Check
                     /note="color: black; sequence: aggtgtctggttcgagatgg; added:
                     2023-05-24"
     CDS             6175..6259
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="*S*SAIPHL*RFYLL*KTSHTSP*T*NI"
     polyA_signal    6282..6403
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    6414..6465
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    6529..6545
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        6553..6583
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    6598..6619
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     CDS             complement(6632..6724)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(join(6907..7453,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     1261 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     1321 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     1381 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     1441 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1501 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1561 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1621 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1681 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1741 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1801 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     1861 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     1921 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     1981 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     2041 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     2101 cttggtaccg agctcggatc gatatctgcg gcctagctag cgcttaaggc ctgttaaccg
     2161 gtatggatgt atgcgtccgt cttgccctgt ggctcctctg gggactcctc ctgcaccagg
     2221 gccagagcct cagccatagt cacagtgaga aggcgacagg aaccagctcg ggcgccggtt
     2281 taaacgaccc tagcaaagac tcaaaagctc aggtgtccgc tgccgaggct ggcattactg
     2341 gaacatggta caatcagctc gggagcacct ttattgtgac tgctggagcc gatggagccc
     2401 tcaccggaac atacgaatct gctgtgggaa acgccgaatc acggtacgtc ctcactggcc
     2461 gatacgatag tgcccctgcc accgacggat ctgggactgc cctgggatgg actgtcgctt
     2521 ggaaaaacaa ctaccggaat gctcattctg ccacaacatg gagtggacag tacgtgggag
     2581 gcgctgaggc tagaatcaat acacagtggc tgctcacatc tggcacaacc gaggcaaatg
     2641 cttggaaatc caccctggtg ggacatgaca cattcaccaa agtgaaaccc tccgccgctt
     2701 caatcgatgc cgccaaaaaa gccggagtca acaacggcaa tcctctggat gccgtccagc
     2761 agctcggacc gaaagatgaa ctgtgagcgg ccgcatagat aactgatcca gtgtgctgga
     2821 attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc
     2881 atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
     2941 cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg
     3001 ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca
     3061 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg
     3121 catctagggc ggccaattcc gcccctctcc ccccccccct ctccctcccc cccccctaac
     3181 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3241 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3301 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3361 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3421 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3481 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3541 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3601 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3661 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3721 cgatgataag cttgccacaa cccgggaggc gcgccatgta caggatgcaa ctcctgtctt
     3781 gcattgcact aagtcttgca cttgtcacga attccgacga gaagaccact ggttggcgag
     3841 gtggacacgt tgttgaagga ctggctgggg aacttgaaca acttcgtgca cgactggagc
     3901 atcacccaca aggtcaacgt gaaccacctg caggtgtgag caagggcgag gaggataaca
     3961 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     4021 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     4081 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     4141 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     4201 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     4261 tgaccgtgac ccaggactcc tccctacagg acggcgagtt catctacaag gtgaagctgc
     4321 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4381 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     4441 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     4501 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     4561 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     4621 gcatggacga gctgtacaag ggccggccag acaagttcac catagttttt ccacacaacc
     4681 aaaaaggaaa ctggaaaaat gttccttcta attaccatta ttgcccgtca agctcagatt
     4741 taaattggca taatgactta ataggcacag ccttacaagt caaaatgccc aagagtcaca
     4801 aggctattca agcagacggt tggatgtgtc atgcttccaa atgggtcact acttgtgatt
     4861 tccgctggta tggaccgaag tatataacac attccatccg atccttcact ccatctgtag
     4921 aacaatgcaa ggaaagcatt gaacaaacga aacaaggaac ttggctgaat ccaggcttcc
     4981 ctcctcaaag ttgtggatat gcaactgtga cggatgccga agcagtgatt gtccaggtga
     5041 ctcctcacca tgtgctggtt gatgaataca caggagaatg ggttgattca cagttcatca
     5101 acggaaaatg cagcaattac atatgcccca ctgtccataa ctctacaacc tggcattctg
     5161 actataaggt caaagggcta tgtgattcta acctcatttc catggacatc accttcttct
     5221 cagaggacgg agagctatca tccctgggaa aggagggcac agggttcaga agtaactact
     5281 ttgcttatga aactggaggc aaggcctgca aaatgcaata ctgcaagcat tggggagtca
     5341 gactcccatc aggtgtctgg ttcgagatgg ctgataagga tctctttgct gcagccagat
     5401 tccctgaatg cccagaaggg tcaagtatct ctgctccatc tcagacctca gtggatgtaa
     5461 gtctaattca ggacgttgag aggatcttgg attattccct ctgccaagaa acctggagca
     5521 aaatcagagc gggtcttcca atctctccag tggatctcag ctatcttgct cctaaaaacc
     5581 caggaaccgg tcctgctttc accataatca atggtaccct aaaatacttt gagaccagat
     5641 acatcagagt cgatattgct gctccaatcc tctcaagaat ggtcggaatg atcagtggaa
     5701 ctaccacaga aagggaactg tgggatgact gggcaccata tgaagacgtg gaaattggac
     5761 ccaatggagt tctgaggacc agttcaggat ataagtttcc tttatacatg attggacatg
     5821 gtatgttgga ctccgatctt catcttagct caaaggctca ggtgttcgaa catcctcaca
     5881 ttcaagacgc tgcttcgcaa cttcctgatg atgagagttt attttttggt gatactgggc
     5941 tatccaaaaa tccaatcgag cttgtagaag gttggttcag tagttggaaa agctctattg
     6001 cctctttttt ctttatcata gggttaatca ttggactatt cttggttctc cgagttggta
     6061 tccatctttg cattaaatta aagcacacca agaaaagaca gatttataca gacatagaga
     6121 tgaaccgact tggaaagtag ggcctcgagg gccaattaat taactctaga taactgatca
     6181 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6241 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc gagtgcattc
     6421 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6481 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7441 cggtggtttt ttt
//
