LOCUS       Exported                6648 bp ds-DNA     circular SYN 23-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 31, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pLH1545_pRS403-pINO4-NLS-PfV-GS-Sapphire  (6648 bp)
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             399..1058
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1156..1838
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    2178..2200
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     2206..2226
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     2244..2263
                     /locus_tag="T3"
                     /label=T3
     promoter        2305..2446
                     /locus_tag="INO4 promoter"
                     /label=INO4 promoter
     CDS             2453..2455
                     /locus_tag="START"
                     /label=START
     misc_signal     2456..2476
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     CDS             2477..3508
                     /locus_tag="PfV"
                     /label=PfV
     misc_signal     3572..4288
                     /locus_tag="Sapphire"
                     /label=Sapphire
     terminator      4309..4496
                     /locus_tag="ADH1 terminator"
                     /label=ADH1 terminator
     primer_bind     complement(4581..4601)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(4610..4627)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             4698..4766
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      4771..5226
                     /locus_tag="M13 origin"
                     /label=M13 origin
     rep_origin      4788..5094
                     /locus_tag="F1 ori"
                     /label=F1 ori
     terminator      complement(5285..5485)
                     /locus_tag="HIS3 terminator"
                     /label=HIS3 terminator
     CDS             complement(5486..6145)
                     /locus_tag="HIS3"
                     /label=HIS3
     CDS             complement(5486..5731)
                     /locus_tag="C terminus of HIS3"
                     /label=C terminus of HIS3
     CDS             complement(5939..6145)
                     /locus_tag="N-terminus of HIS3"
                     /label=N-terminus of HIS3
     promoter        complement(6146..6332)
                     /locus_tag="HIS3 promoter"
                     /label=HIS3 promoter
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctct
     2281 cttggcttgg tttgtatgag cttgttacgt atcaaacaaa atattcacat gtttttctca
     2341 ccttaaagac agaggcgcga ttgcgtttgc gaaattccaa tgccaacaag ataagcttga
     2401 cgaaggagtt aagagggcgg cttgaactaa aaagagaaaa gcaatatcta gaatgccaaa
     2461 aaagaagaga aaggtcctct caataaatcc aacccttata aatagggata agccatacac
     2521 caaagaagaa ttaatggaaa ttctgagatt agcaataata gctgagcttg atgcaataaa
     2581 tctatatgag cagatggcaa gatattccga agatgaaaac gtaagaaaga ttctccttga
     2641 tgttgcaagg gaggagaagg ctcacgttgg agaattcatg gcactccttc taaatttgga
     2701 tccagaacaa gtaactgagc tgaagggtgg atttgaggaa gtcaaggagc taactggaat
     2761 tgaggctcac ataaatgata acaagaagga ggaatctaac gtggaatatt ttgagaagtt
     2821 gagaagtgct cttttggatg gggtaaataa gggaagatct ttactaaagc acttgccagt
     2881 aacgagaata gaaggacaat cattcagggt tgacatcatt aaatttgaag atggtgttag
     2941 ggtagtaaag caagagtaca agccaattcc attattgaag aagaaattct atgtgggaat
     3001 aagagaactc aacgatggga cttacgatgt atctatagca actaaagctg gggaactcct
     3061 agttaaagac gaagagtcgc tagtaataag agaaattcta tccacagagg gaattaagaa
     3121 aatgaaactt tcctcctggg acaatccaga agaagcactt aatgatttaa tgaacgcact
     3181 gcaggaagcc tcaaatgcgt cagctggacc atttgggctg ataattaacc caaaaagata
     3241 tgcaaagttg ctaaaaattt acgagaagag tggaaagatg ctagttgaag tgctaaaaga
     3301 gatattcagg ggaggaatca ttgttactct aaacattgat gagaataagg taataatatt
     3361 tgccaataca ccagctgttc ttgacgttgt agttggccaa gatgtgactc tacaagagct
     3421 aggacctgaa ggagacgacg ttgcattctt ggttagtgaa gctataggaa taagaataaa
     3481 gaacccagag gcaatagtag tcttagagtg gtctggcagc ggtagtgggt cgggatcagg
     3541 ctccggtcga cggatccccg ggttaattaa gatgtctaaa ggtgaagaat tattcactgg
     3601 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     3661 cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac
     3721 tggtaaattg ccagttccat ggccaacctt agtcactact ttttcttatg gtgttcaatg
     3781 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     3841 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     3901 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     3961 taaagaagat ggtaacattt taggtcacaa attggaatac aactttaact ctcacaatgt
     4021 ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa
     4081 cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga
     4141 tggtccagtc ttgttaccag acaaccatta cttatccatt caatctgcct tatccaaaga
     4201 tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg ctggtattac
     4261 ccatggtatt gatgaattgt acaaataagg cgcgccactt ctaaataagc gaatttctta
     4321 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     4381 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     4441 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
     4501 tccgctaggg ataacagggt aatatagatc tgtttagctt gcctcgtccc cgctcgaggg
     4561 ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     4621 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4681 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4741 agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg
     4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4981 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5101 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     5221 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     5281 tccgtcgagt tcaagagaaa aaaaaagaaa aagcaaaaag aaaaaaggaa agcgcgcctc
     5341 gttcagaatg acacgtatag aatgatgcat taccttgtca tcttcagtat catactgttc
     5401 gtatacatac ttactgacat tcataggtat acatatatac acatgtatat atatcgtatg
     5461 ctgcagcttt aaataatcgg tgtcactaca taagaacacc tttggtggag ggaacatcgt
     5521 tggtaccatt gggcgaggtg gcttctctta tggcaaccgc aagagccttg aacgcactct
     5581 cactacggtg atgatcattc ttgcctcgca gacaatcaac gtggagggta attctgctag
     5641 cctctgcaaa gctttcaaga aaatgcggga tcatctcgca agagagatct cctactttct
     5701 ccctttgcaa accaagttcg acaactgcgt acggcctgtt cgaaagatct accaccgctc
     5761 tggaaagtgc ctcatccaaa ggcgcaaatc ctgatccaaa cctttttact ccacgcgcca
     5821 gtagggcctc tttaaaagct tgaccgagag caatcccgca gtcttcagtg gtgtgatggt
     5881 cgtctatgtg taagtcacca atgcactcaa cgattagcga ccagccggaa tgcttggcca
     5941 gagcatgtat catatggtcc agaaacccta tacctgtgtg gacgttaatc acttgcgatt
     6001 gtgtggcctg ttctgctact gcttctgcct ctttttctgg gaagatcgag tgctctatcg
     6061 ctaggggacc accctttaaa gagatcgcaa tctgaatctt ggtttcattt gtaatacgct
     6121 ttactagggc tttctgctct gtcatctttg ccttcgttta tcttgcctgc tcatttttta
     6181 gtatattctt cgaagaaatc acattacttt atataatgta taattcatta tgtgataatg
     6241 ccaatcgcta agaaaaaaaa agagtcatcc gctaggggaa aaaaaaaaat gaaaatcatt
     6301 accgaggcat aaaaaaatat agagtgtact agaggaggcc aagagtaata gaaaaagaaa
     6361 attgcgggaa aggactgtgt tatgacttcc ctgactaatg ccgtgttcaa acgatacctg
     6421 gcagtgactc ctagcgctca ccaagctctt aaaacgggaa tttatggtgc actctcagta
     6481 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     6541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
