LOCUS       Exported                5536 bp ds-DNA     circular SYN 23-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 31, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pLH1459_pINO4-NLS-Pfv-Sapphire-HO.dna  (5536 bp)
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(232..820)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
     CDS             1115..1975
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(2238..2707)
                     /locus_tag="HO downstream"
                     /label=HO downstream
     promoter        2745..2886
                     /locus_tag="INO4 promoter"
                     /label=INO4 promoter
     CDS             2893..2895
                     /locus_tag="PfV(1)"
                     /label=PfV
                     /label=PfV(1)
     misc_signal     2896..2916
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     CDS             2917..3948
                     /locus_tag="PfV"
                     /label=PfV
     misc_signal     3952..3984
                     /locus_tag="GS linker"
                     /label=GS linker
     misc_signal     4012..4728
                     /locus_tag="Sapphire"
                     /label=Sapphire
     terminator      4749..4936
                     /locus_tag="ADH1 terminator"
                     /label=ADH1 terminator
     CDS             complement(4993..5492)
                     /locus_tag="HO upstream"
                     /label=HO upstream
ORIGIN
        1 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
       61 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
      241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      961 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     1021 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1081 atctatcaac aggagtccaa gcgagctcga tatcatgagt attcaacatt tccgtgtcgc
     1141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1801 tgctgataaa tctggagccg gtgagcgtgg atctcgcggt atcattgcag cactggggcc
     1861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatttct
     1981 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2041 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
     2101 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2161 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     2221 cggccgcgta tccttgccta acttcaatgt caaagttgaa atattctcct ttagagcgct
     2281 ccatttcttc tatgaagcgt tttgcggcaa actcaccttc aactgtcatt gggaatgtct
     2341 tatgatggtt ttttggaatt attattatcc taccatcaag cgtctgacat tgctgcagat
     2401 ttctccatct cactttatat ttggtggcat ttctaccact tttttccaac agtggtttgg
     2461 tagggaccct gactgacaat ttatgacctg cagtacattg taatgcaaga cgctgataaa
     2521 ctgttctacg cctgggatct aacctaccag gttcaccttc aaaagctctg tgtttggttt
     2581 tttgctgtat attatagatt ttctgatagc cctgtgtgac atttatgacg cgggcagcgg
     2641 agccatctgc gcacataacg taagagttag ccgtgacgtt tgcgatgtct ttaatttcac
     2701 cgttagcgaa ttggagctct cttggcttgg tttgtatgag cttgttacgt atcaaacaaa
     2761 atattcacat gtttttctca ccttaaagac agaggcgcga ttgcgtttgc gaaattccaa
     2821 tgccaacaag ataagcttga cgaaggagtt aagagggcgg cttgaactaa aaagagaaaa
     2881 gcaatatcta gaatgccaaa aaagaagaga aaggtcctct caataaatcc aacccttata
     2941 aatagggata agccatacac caaagaagaa ttaatggaaa ttctgagatt agcaataata
     3001 gctgagcttg atgcaataaa tctatatgag cagatggcaa gatattccga agatgaaaac
     3061 gtaagaaaga ttctccttga tgttgcaagg gaggagaagg ctcacgttgg agaattcatg
     3121 gcactccttc taaatttgga tccagaacaa gtaactgagc tgaagggtgg atttgaggaa
     3181 gtcaaggagc taactggaat tgaggctcac ataaatgata acaagaagga ggaatctaac
     3241 gtggaatatt ttgagaagtt gagaagtgct cttttggatg gggtaaataa gggaagatct
     3301 ttactaaagc acttgccagt aacgagaata gaaggacaat cattcagggt tgacatcatt
     3361 aaatttgaag atggtgttag ggtagtaaag caagagtaca agccaattcc attattgaag
     3421 aagaaattct atgtgggaat aagagaactc aacgatggga cttacgatgt atctatagca
     3481 actaaagctg gggaactcct agttaaagac gaagagtcgc tagtaataag agaaattcta
     3541 tccacagagg gaattaagaa aatgaaactt tcctcctggg acaatccaga agaagcactt
     3601 aatgatttaa tgaacgcact gcaggaagcc tcaaatgcgt cagctggacc atttgggctg
     3661 ataattaacc caaaaagata tgcaaagttg ctaaaaattt acgagaagag tggaaagatg
     3721 ctagttgaag tgctaaaaga gatattcagg ggaggaatca ttgttactct aaacattgat
     3781 gagaataagg taataatatt tgccaataca ccagctgttc ttgacgttgt agttggccaa
     3841 gatgtgactc tacaagagct aggacctgaa ggagacgacg ttgcattctt ggttagtgaa
     3901 gctataggaa taagaataaa gaacccagag gcaatagtag tcttagagtg gtctggcagc
     3961 ggtagtgggt cgggatcagg ctccggtcga cggatccccg ggttaattaa gatgtctaaa
     4021 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
     4081 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
     4141 ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
     4201 ttttcttatg gtgttcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt
     4261 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
     4321 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
     4381 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
     4441 aactttaact ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt
     4501 aacttcaaaa ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa
     4561 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttatccatt
     4621 caatctgcct tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
     4681 gttactgctg ctggtattac ccatggtatt gatgaattgt acaaataagg cgcgccactt
     4741 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     4801 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     4861 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg
     4921 accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc tgtttagctt
     4981 gcctcgtcat gctttaaagt atagatagaa ttgattgctg cttatgagga tatggattta
     5041 gagctaatag ttgtaataga aataaaagcc atttagaata ggaattgaat acaattttat
     5101 gagctttaaa tatttaatga tgaatagtgc gtttagaacg cttcatcatg cttcaacaag
     5161 cttctgcaaa cctcatttga gtagcaatag ctgtttactt ttttccgcga ctttattcga
     5221 agttatggtg aacttttcgt ggtttcacac ctaataacgc ccagcggtag tacaattcaa
     5281 agtagtaggt accaatggta gtactagtgg gtgatagtag caaatttctc tatgaaaatg
     5341 aattgttgct ctgcaatgat gttaatgtgg acatgacatg aattttcgcg atatgttgat
     5401 gtaaataata acatgatttt cgtggatcat ttcgtaaacc ataggtttat ttcgtgaatt
     5461 actaagagaa aggcatagaa acaggacttg cggatgggat acgcggccgc tgcagtccgg
     5521 caaaaaaggg caaggt
//
