LOCUS       Exported                7148 bp ds-DNA     circular SYN 04-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAG eGFP-Oct6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   David Rodda
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     http://www.informaxinc.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1715
                     /label=CAG promoter
                     /label=CAG\promoter
                     /note="/vntifkey=29"
     promoter        1..366
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=29"
     primer_bind     1586..1608
                     /label=pCAGGS-5
     primer_bind     1658..1676
                     /label=pCAGseqF
                     /note="/vntifkey=28"
     primer_bind     1671..1694
                     /label=6CAG-F
                     /note="/vntifkey=28"
     CDS             1729..2445
                     /codon_start=1
                     /label=eGFP A206K
                     /label=eGFP\A206K
                     /note="/vntifkey=4"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2426..2445
                     /label=EGFP-terminal-F
     misc_feature    2446..2457
                     /label=Linker
                     /note="/vntifkey=21"
     misc_feature    2458..3813
                     /label=Mm.Oct6
     misc_feature    2562..2567
                     /label=insert from sequencing
     CDS             3772..3789
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3895..3914
                     /label=pCAGseqR2
                     /note="/vntifkey=28"
     polyA_signal    3967..4017
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="/vntifkey=25"
     misc_binding    4064..4519
                     /label=f1 origin
                     /label=f1\origin
                     /note="/vntifkey=20"
     promoter        4693..4995
                     /label=SV40ori/SV40 Promoter
                     /label=SV40ori/SV40\Promoter
                     /note="/vntifkey=30"
     misc_feature    5044..5838
                     /label=Kan/Neo
                     /label=nonstandard type:  misc_marker
                     /note="/vntifkey=22"
     polyA_signal    6074..6092
                     /label=HSVTK polyA
                     /label=HSVTK\polyA
                     /note="/vntifkey=25"
     misc_binding    6423..7066
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=20"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      421 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      481 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      541 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      601 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc
      661 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      721 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      781 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      841 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      901 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      961 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     1021 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1081 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     1141 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1201 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1261 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1321 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1381 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1441 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1501 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1561 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1621 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattgagct ccacaaccat ggtgagcaag
     1741 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1801 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1861 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1921 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1981 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2041 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2101 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2161 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     2221 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2281 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2341 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2401 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg cagcggcatg
     2461 gccaccaccg cgcagtatct gccgcggggc cccggcggcg gagctggggg cacagggccg
     2521 ctcatgcatc ccgatgccgc cgcggcggcg gcagcggcgg cggcggccga gcggctgcac
     2581 gcgggggccg cgtaccgcga agtgcagaag ctgatgcacc acgagtggct gggcgcgggc
     2641 gcgggccacc ccgtgggcct agcgcaccct caatggctac ccacgggagg aggcggcggc
     2701 ggcgactggg cgggcggccc gcacctggaa cacggcaagg caggcggtgg cggtaccggc
     2761 cgagctgacg acggcggcgg tggcggcggt ttccacgccc gcctggtgca ccaaggggcg
     2821 gcccacgcgg gcgcggcatg ggcacaaggc ggcacagcgc accacttggg ccccgccatg
     2881 tcgccgtcgc ccggggccgg cgggggtcac cagccccagc cgctcgggct gtacgctcag
     2941 gcggcctacc ccggtggcgg cggcggcggc ctggccggga tgctggcggc gggaggcggc
     3001 ggcgcgggac ccggcctgca ccacgcactg cacgaggacg gccacgaggc acagctggag
     3061 ccgtcgccac caccgcacct gggcgcacac ggacacgcac acggacatgc acacgcgggc
     3121 ggcctgcacg cggcggcggc gcacctgcac ccgggcgcgg gcggtggtgg ctcgtcggtg
     3181 ggcgagcact cggacgagga tgctcccagc tccgacgacc tggagcagtt cgccaagcag
     3241 ttcaagcaac gacgcatcaa gctgggcttc acccaggccg acgtgggact ggcgctgggc
     3301 accctctacg gtaacgtgtt ctcgcagacc accatctgcc gtttcgaggc cctgcagctg
     3361 agcttcaaga acatgtgcaa gctcaagccg ctgctcaaca agtggctgga ggagaccgac
     3421 tcgtccagcg gcagccccac caacctggac aagatcgcgg cgcagggccg caagcgcaag
     3481 aagcgcacgt ccatcgaggt gggtgtcaaa ggcgcgctcg agagccactt tctcaagtgt
     3541 cccaagccgt ctgcgcacga gatcaccggc ctggccgaca gcctgcaact ggagaaggag
     3601 gtggtgcgtg tctggttctg caaccggcgg cagaaggaga agcgcatgac ccccgcggcc
     3661 ggcgcgggcc acccgcccat ggacgacgtt tatgcgcctg gggagctggg gcctggcggg
     3721 ggcagcgcgt cgccaccttc tgcgcccccg ccacccccgc cggccgcgct gcaccaccac
     3781 caccaccaca cactgcccgg ctctgtgcag tgaagcggcc gcgactctag atcataatca
     3841 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3901 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     4081 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4141 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4201 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4261 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4321 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4381 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4441 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4501 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4621 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     4681 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4801 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4921 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4981 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     5041 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5101 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5161 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5221 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5281 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5341 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5401 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5461 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5521 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     5581 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5641 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5701 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5761 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5821 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5881 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5941 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6001 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     6061 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     6121 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     6181 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     6241 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     6301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7141 ccatgcat
//
