LOCUS       Exported                8183 bp ds-DNA     circular SYN 08-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    15..174
                     /label=5' Tol2  sequence
                     /note="5' Tol2  sequence"
     misc_feature    305..4304
                     /label=4kb wnt3 promoter
     misc_feature    4332..5051
                     /label=EGFP
                     /note="EGFP"
     misc_feature    5061..5140
                     /label=3' Tol2 sequence
                     /note="3' Tol2 sequence"
     misc_feature    complement(5308..5326)
                     /label=SP6 Promoter
                     /note="SP6 Promoter"
     misc_feature    complement(5344..5360)
                     /label=M13 Rev
                     /note="M13 Rev"
     misc_feature    5368..5384
                     /label=Lac operator
                     /note="Lac operator"
     misc_feature    complement(5392..5422)
                     /label=Lac Promoter
                     /note="Lac Promoter"
     misc_feature    5437..5458
                     /label=CAP binding site
                     /note="CAP binding site"
     misc_feature    6505..7365
                     /label=Amp Resistance
                     /note="Amp Resistance"
     misc_feature    8144..8160
                     /label=M13 Forward
                     /note="M13 Forward"
     misc_feature    8167..8183
                     /label=T7 Promoter
                     /note="T7 Promoter"
ORIGIN
        1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
       61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
      121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
      181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
      241 aaccaattga attgcgctga tgcccagttt aatttaaata gatctggcca tctagagcaa
      301 gcttcaataa aagaagtgtc tgttgtgggg ccgagggtcc tggggggctg cggagcttta
      361 ggcattgtcc tggagggtga ttgagagcga gaggagggga gccgagaagc attgtgcgag
      421 gacggagcct ttctaaaggt ggttattagg agagaaatgg tgtgggggtg gctgttgact
      481 cttcatcttg tgtgaacaaa gtatctggcg tatagctttc tctctctctc tctcgctctc
      541 ttgcggcttt cgctttacga cggccatgaa tactctcggc gggctcgccc agacctgcgc
      601 gaaggcttat tgtccggctt aagtgcggtt taattaagcg agctctgtgt cctgagagaa
      661 gagaggagct ttagagcgct cgggtcctgc ggagatgcag aggatgatgc gtaatatcag
      721 ggctattgaa ggggaaagtg tttgcggctc taatgaaaaa ggtttgtttg ggggggatga
      781 gtggggaggg gattgaggag tgtctggcct ttgtctccag cttacaaagt agggacaaaa
      841 gcagtgatat tatacaaatg cacccatttc caggacatga tgcgaggacg ggggcctgga
      901 gatgggtcgg actgctccat ctcagccccg ttggcacctt acaaaatgat tttttccccc
      961 caccacaaag ataggcattg gggataaacc ccgagagctg gggcttctgg gtaccgcaaa
     1021 tttgggggtt gtggacttaa gcggattttc tctgcaactg taagaacaag ctaattcctc
     1081 agacctcctt tatctctaac atggtaataa caaacaatga aattctgaag agaaaggggc
     1141 aaaggaagag gagggaggag agacgcagac acaaaagaag gagagatgga gggaatgaaa
     1201 gagaggagag acgtgtgtga ggagagggtg ggcttgggcg tgttaagaaa ctttctcgct
     1261 ctagtgttat gtgtgaacac tctgctaatc caggttttat tgcttgcctg tgtccggcca
     1321 cactgtccgg attgagtgtg tgtcagggat agtgggggtc tacacatgtc cctctttgag
     1381 gaaagcctga gaaagagccg gacctggtta cggccgtttt ggggtcgcct aagaaacctg
     1441 caccacaggg gtcctctgtg tatgtctctg aattcaacgt gcatgttgct agcaaacaca
     1501 tacggattaa tgctaatgat gaaggtgtgg ctatattagg tacaaaaaaa aaaactttga
     1561 caatagttgt ataaactcct gtcaacagtc catcaatgta tgaagcagaa ctcgctttga
     1621 aaccaaacat tgttgtgtgg gtccatttgg accagtttaa ggaaatcttt tgccctataa
     1681 attccagcct gcatgaattt ttatgattat tgctcataaa catactttta aaaatgtgca
     1741 ttttaactaa ctatcaactt gtgcccgtcc actcaaagga agccaaaagg gtgtctctcc
     1801 cagatcacag ggactacttt gtgggtgtgt agggtgatgt attagctagg cgctaataaa
     1861 atgcaggagt gtccacaaac atgacttaat gagggaggat gagagactcc tgacccagag
     1921 gtcaaagctc cgggcgactc tctttattcc cattgatctg ctggtgcact tcatttccat
     1981 gctctagggc ccacttcagt aaattcatta agtgcgattg atacgagaat caagacttcc
     2041 tccaaaacac aatggctatg ctaggaggga atggtcttgt ttgtgctgca gctatgtcaa
     2101 gcatctctgg cttttgtcct ggtttcgcac cattgcggtg catgctgggg tttgagtcct
     2161 cgagattcta atcgtaagct catttccagc tggttcgttg tgttgcgccc ctatggcctt
     2221 gacctgggaa acgtggtttc agtgttgttg agatgccgac cgcagaccgt tccttgcctc
     2281 cactcgtaaa ttacgctccg atcaggtttt cccagccaat cctggcgagc agatctgcat
     2341 ctgtcactct gcaagaatgc tgaatcaaac cgctgatcta ggcacaatac agctgatgga
     2401 aagcagatct ttattccaac atcaggaacg tagcacgagc cgagtgcttc tggaagctgc
     2461 aatttaacca acaggttcat atctgatgag atcaaggatg catcatcctg gaagaaacac
     2521 tgtgatgaac atttaagatg tcagaatgac ccgtcatgcc tctgagctga gtccccgcac
     2581 agcgcttatt cttttgaaga gggagaataa aagcaaaaaa acagaggcga tggaaatatg
     2641 acatccagac cgcggtcaga actgtgagtg tctctcctgg gctcctatgg ttgtggtgca
     2701 cagtccatgt ccctgctatg agttagatag acagatgcac tggcagatag acagacagac
     2761 aggctttact acttggtcgc tggattgcag gaaagagctt tatccaacca gagtcaagca
     2821 agcaacatgg gacaataaca taattaaata ttctggggaa aagtggcact tatgcaaatg
     2881 ttttttgtaa tgggttggag aaaattggcc ggaacctaca taatttttaa tgacaggatg
     2941 attcatttgt gaaattgtaa ttcaaagaaa ttcagtaaac taactaacaa tctaaaaaat
     3001 tctttgattt gcagtttaat tctacttcct aacattgatt tgtttcaact ttattcaaat
     3061 ggcaaaacta aattagtact caaagctcca aatgatatga ttcctagact gtttaaagtg
     3121 ctaaaagtgc caaaacttac agctacccac atgaacttat atttcattct gcctccattt
     3181 gcaattcctc cagtgtttgt acctgaaact gttgtcagtt ctcacctctg tcctgccgtt
     3241 cattctgaca cagagactct atatttgaat gtattgtcaa cttctaatgc cgtgtctact
     3301 actctgtgct gtctgctctc atcccatccg aatgacaata gtgtttatgt aaaatgtgct
     3361 gcctttgggc cgctggggca cagaacactg catgcctggc cctgccacac caccttttac
     3421 ggctttttat tgtgttgcct ttgtgtcaca aggggaggac gcggccatca aaattctcca
     3481 agacaaaatt cttgaatgta tgtgcacgca cacgcatccg gaacaattag ttcaggagca
     3541 cgggtgctta aaaaacggag acgacacttc tcgccaccac ctcctcgccg agcagcaggc
     3601 ccgagtctgg cacagctggg tttccttgat gctaacgcag acaatataga tcttaataag
     3661 acaaatctac ctctccgaaa ctcagtcctg acaccctttc attcttattc cctcccagcc
     3721 tcctctctct ctctctctct ctttctttct ttctttgcta cattaccagg gggtggtgca
     3781 tgattgacag atgggcagga ataataattt tttttttttt tttgcgtaac ctaaggccat
     3841 gctctgtgat tggcttgcca gctgacatct ccaaacctct cttgtcgttt ttttcccctc
     3901 tctctctctc gctttctctc tctcactctc tctctctaat attggcsctg atgatcctaa
     3961 ggggaaaaaa gtggggagag aaaaagatat gtaccttagg gaagaacctc agtaagaggg
     4021 agggaatcag aggacgagta ggagcgtgtg tgcgagtgtg tgaaagagac acttactctc
     4081 ctaactacta ctggaagaca cacacttttc tcacccaact caaagcagca gcaaaccttg
     4141 gagaattgtt ttatttttgt tttttcgcga accccccyaa aaaaaattcc taattattct
     4201 ctcaagcatc catctttgcc ttgccgggat ttaattttca ccccctctct aggaatccta
     4261 tgtactgatc aatagaccct ttccatccac acaagacata cctccgcgga tccgatccac
     4321 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4381 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4441 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4501 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4561 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4621 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4681 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4741 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4801 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4861 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4921 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4981 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5041 tgtacaagta aagcggccgc aaacaagaat ctctagtttt ctttcttgct tttactttta
     5101 cttccttaat actcaagtac aattttaatg gagtactttt ttacttttac tcaagtaaga
     5161 ttctagccag atacttttac ttttaattga gtaaaatttt ccctaagtac ttgtactttc
     5221 acttgagtaa aatttttgag tactttttac acctctgctc gaccatatgg gagagctccc
     5281 aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc
     5341 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5401 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5461 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5521 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6181 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc
     7501 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     7561 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7621 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7681 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7741 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7801 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7861 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7921 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7981 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     8041 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     8101 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     8161 gaattgtaat acgactcact ata
//
