LOCUS       addgene-plasmid-        9274 bp DNA     circular SYN 03-NOV-2023
DEFINITION  Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter 
            driving the expression of Wnt3-EGFP for zebrafish transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    pminiTol2-4kb wnt3 promoter-Wnt3EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Teh C, Sun G, Shen H, Korzh V, Wohland T
  TITLE     Modulating the expression level of secreted Wnt3 influences 
            cerebellum development in zebrafish transgenics.
  JOURNAL   Development. 2015 Nov 1;142(21):3721-33. doi: 10.1242/dev.127589. 
            Epub 2015 Sep 22.
  PUBMED    26395493
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 3, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.127589"; journalName: "Development"; date: "2015-11-1- 
            1"; volume: "142"; issue: "21"; pages: "3721-33"
FEATURES             Location/Qualifiers
     source          1..9274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..275
                     /label=5'Tol2 DNA
                     /note="5'Tol2 DNA"
                     /note="color: #ff80ff"
     misc_feature    276..298
                     /label=MCS
                     /note="MCS"
                     /note="color: #0000ff"
     misc_feature    297..304
                     /label=Not1
                     /note="Not1"
                     /note="color: #ffff80"
     CDS             complement(309..1025)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 3 segments:
                        1:  309 .. 1019 / #05fc14
                        2: 1020 .. 1022 / #05fc14 / 1a
                        3: 1023 .. 1025 / #05fc14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(351..372)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     700..719
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     959..980
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     regulatory      1022..1031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    1026..2129
                     /label=zf Wnt3
                     /note="color: #ccffcc"
     regulatory      2126..2135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    2148..6145
                     /label=4kb wnt3 promoter
                     /note="color: #a6acb3"
     misc_feature    2148..2197
                     /label=3' 4kb wnt3 pro
                     /note="color: #a6acb3"
     misc_feature    2597..2604
                     /label=TCF BS (GATTGGCT)
                     /note="color: #99cc00"
     misc_feature    2673..2714
                     /label=end of 4kb-secmApple promoter
                     /note="color: #a6acb3"
     misc_feature    2676..2683
                     /label=SP5 BS (GGGGGTGG)
                     /note="color: #993300"
     misc_feature    3083..3090
                     /label=TCF BS (CCTTTGGG)
                     /note="color: #ffcc00"
     misc_feature    3557..3569
                     /label=C/EBP (TTTTGTAATGGGt)
                     /note="color: #a6acb3"
     misc_feature    3687..3700
                     /label=SMAD3-SMAD4 (CAGACAGACAGgCt)
                     /note="color: #cc99ff"
     misc_feature    3695..3708
                     /label=SMAD3-SMAD4 (CAGAtAGACAGACA)
                     /note="color: #cc99ff"
     misc_feature    4131..4138
                     /label=TCF BS (GATTGGCT)
                     /note="color: #99cc00"
     misc_feature    4738..4751
                     
                     /note="color: #cc99ff"
     misc_feature    5063..5076
                     /label=ETF-A(GGCTTTCcTCAaAG)
                     /note="color: #cc99ff"
     misc_feature    5691..5704
                     /label=PDX1 (GGCTCTAATGAaAa)
                     /note="color: #a6acb3"
     misc_feature    5979..5986
                     /label=SP5 BS (GGGGGTGG)
                     /note="color: #993300"
     misc_feature    6088..6145
                     /label=5' 4kb wnt3 pro
                     /note="color: #a6acb3"
     misc_feature    6152..6348
                     /label=3' Tol2 DNA
                     /note="3' Tol2 DNA"
                     /note="color: #80ffff"
     promoter        complement(6399..6417)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6400..6417)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
                     /note="color: black; sequence: ATTTAGGTGACACTATAG"
     primer_bind     complement(6435..6451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6435..6451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(6448..6470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    6459..6475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6483..6513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6483 .. 6489 / #ffffff / -10
                        2: 6490 .. 6507 / #ffffff
                        3: 6508 .. 6513 / #ffffff / -35"
     protein_bind    6528..6549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(6666..6683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(6837..7425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(6917..6936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(7596..8456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7596 .. 8387 / #ccffcc
                        2: 8388 .. 8456 / #ccffcc / signal sequence
                     Cleavage site after base 8387"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8219..8238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(8457..8561)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(8639..9094)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(8776..8797)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     8988..9007
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     9220..9242
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     9234..9251
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     9235..9251
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     join(9258..9274,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        join(9258..9274,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
       61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
      121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
      181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
      241 aaccaattga attgcgctga tgcccagttt aatttaaata gatctggcca tctagagcgg
      301 ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      361 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
      421 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      481 tggtcggcga gctgcacgct gccgtcctcg atgttgtgac ggatcttgaa gttcaccttg
      541 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      601 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      661 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      721 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      781 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
      841 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      901 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
      961 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     1021 accatggtgg cgaccggccg gtggatcccg ggcccgcggt accgtttaca tgtatgtacg
     1081 tcgtagaccc tcacacattc ctggcagcta acgtaacagc accagtggaa gatgcagtgg
     1141 catttctctt tgcgcttttc tgtccgtgtg ttatggccac ggccacagca cagcaggtca
     1201 cagccttcga tgccgtgcga cgacacgttg caggcacggt cacgggtgcc gaatgagccc
     1261 gtctctgggt ttggctcgca gaagttgggt gatccctcat agtataccag gtctcgctca
     1321 gtgggatgct tgaagaaggc gtatttggct cgtagtgttt ccacccagcc tctggactcc
     1381 cggtgtttct ccaccaccat ctcagaggcg ctgtcgtact tgtctttcag gtagtccccc
     1441 aacagtctga agtcaggctg cgcccaccag cacgtcttca cttcacagct gcctgacagc
     1501 ccatggcatt tacagcgcag gtgcatgttc tccaatatgg tcattcgtcc tgcctcattg
     1561 ttgtgcctgt tcatggcact ccgagcatct ggccggttct cacgagcgtc tgcaaactct
     1621 cgagatacca aaaccccaaa ttcagcatct tcactgcagc caccccactt ccatccttct
     1681 cccggtgggc ctttatgatg ggagtcacag ccacacatag tggaggtccc ctctgcacat
     1741 gaacgggtta ctgcaaatgc tactccagct gaggcaatcg catgaacaaa tgcagattcc
     1801 cttgtagctt tatcaagcac tggtccaaat attgctagac tgtccttgat agtggtgcag
     1861 ttccacctgc gaccacggaa ctggtgctga cactcctgga tacccagttt gactccttcc
     1921 gctacactgg gcatgatctc tatgtaattg cggcagaagc gcagctgttt tggcaccagg
     1981 ccaggtatgg agccacacag gatgggttgg gatccaatgg atgagtactg ctgcccaagg
     2041 gccagggacc accagatggg atagcctcca agcacccgag agctggagaa ccacatgcac
     2101 atgagaaatc caaccaggta caaatccatg gtggcgaccg gtggatccga ggtatgtctt
     2161 gtgtggatgg aaagggtcta ttgatcagta cataggattc ctagagaggg ggtgaaaatt
     2221 aaatcccggc aaggcaaaga tggatgcttg agagaataat taggaatttt tttagggggg
     2281 ttcgcgaaaa aacaaaaata aaacaattct ccaaggtttg ctgctgcttt gagttgggtg
     2341 agaaaagtgt gtgtcttcca gtagtagtta ggagagtaag tgtctctttc acacactcgc
     2401 acacacgctc ctactcgtcc tctgattccc tccctcttac tgaggttctt ccctaaggta
     2461 catatctttt tctctcccca cttttttccc cttaggatca tcagggccaa tattagagag
     2521 agagagtgag agagagaaag cgagagagag agaggggaaa aaaacgacaa gagaggtttg
     2581 gagatgtcag ctggcaagcc aatcacagag catggcctta ggttacgcaa aaaaaaaaaa
     2641 aaaattatta ttcctgccca tctgtcaatc atgcaccacc ccctggtaat gtagcaaaga
     2701 aagaaagaaa gagagagaga gagagagagg aggctgggag ggaataagaa tgaaagggtg
     2761 tcaggactga gtttcggaga ggtagatttg tcttattaag atctatattg tctgcgttag
     2821 catcaaggaa acccagctgt gccagactcg ggcctgctgc tcggcgagga ggtggtggcg
     2881 agaagtgtcg tctccgtttt ttaagcaccc gtgctcctga actaattgtt ccggatgcgt
     2941 gtgcgtgcac atacattcaa gaattttgtc ttggagaatt ttgatggccg cgtcctcccc
     3001 ttgtgacaca aaggcaacac aataaaaagc cgtaaaaggt ggtgtggcag ggccaggcat
     3061 gcagtgttct gtgccccagc ggcccaaagg cagcacattt tacataaaca ctattgtcat
     3121 tcggatggga tgagagcaga cagcacagag tagtagacac ggcattagaa gttgacaata
     3181 cattcaaata tagagtctct gtgtcagaat gaacggcagg acagaggtga gaactgacaa
     3241 cagtttcagg tacaaacact ggaggaattg caaatggagg cagaatgaaa tataagttca
     3301 tgtgggtagc tgtaagtttt ggcactttta gcactttaaa cagtctagga atcatatcat
     3361 ttggagcttt gagtactaat ttagttttgc catttgaata aagttgaaac aaatcaatgt
     3421 taggaagtag aattaaactg caaatcaaag aattttttag attgttagtt agtttactga
     3481 atttctttga attacaattt cacaaatgaa tcatcctgtc attaaaaatt atgtaggttc
     3541 cggccaattt tctccaaccc attacaaaaa acatttgcat aagtgccact tttccccaga
     3601 atatttaatt atgttattgt cccatgttgc ttgcttgact ctggttggat aaagctcttt
     3661 cctgcaatcc agcgaccaag tagtaaagcc tgtctgtctg tctatctgcc agtgcatctg
     3721 tctatctaac tcatagcagg gacatggact gtgcaccaca accataggag cccaggagag
     3781 acactcacag ttctgaccgc ggtctggatg tcatatttcc atcgcctctg tttttttgct
     3841 tttattctcc ctcttcaaaa gaataagcgc tgtgcgggga ctcagctcag aggcatgacg
     3901 ggtcattctg acatcttaaa tgttcatcac agtgtttctt ccaggatgat gcatccttga
     3961 tctcatcaga tatgaacctg ttggttaaat tgcagcttcc agaagcactc ggctcgtgct
     4021 acgttcctga tgttggaata aagatctgct ttccatcagc tgtattgtgc ctagatcagc
     4081 ggtttgattc agcattcttg cagagtgaca gatgcagatc tgctcgccag gattggctgg
     4141 gaaaacctga tcggagcgta atttacgagt ggaggcaagg aacggtctgc ggtcggcatc
     4201 tcaacaacac tgaaaccacg tttcccaggt caaggccata ggggcgcaac acaacgaacc
     4261 agctggaaat gagcttacga ttagaatctc gaggactcaa accccagcat gcaccgcaat
     4321 ggtgcgaaac caggacaaaa gccagagatg cttgacatag ctgcagcaca aacaagacca
     4381 ttccctccta gcatagccat tgtgttttgg aggaagtctt gattctcgta tcaatcgcac
     4441 ttaatgaatt tactgaagtg ggccctagag catggaaatg aagtgcacca gcagatcaat
     4501 gggaataaag agagtcgccc ggagctttga cctctgggtc aggagtctct catcctccct
     4561 cattaagtca tgtttgtgga cactcctgca ttttattagc gcctagctaa tacatcaccc
     4621 tacacaccca caaggtagtc cctgtgatct gggagagaca cccttttggc ttcctttgag
     4681 tggacgggca caagttgata gttagttaaa atgcacattt ttaaaagtat gtttatgagc
     4741 aataatcata aaaattcatg caggctggaa tttatagggc aaaagatttc cttaaactgg
     4801 tccaaatgga cccacacaac aatgtttggt ttcaaagcga gttctgcttc atacattgat
     4861 ggactgttga caggagttta tacaactatt gtcaaagttt ttttttgtac ctaatatagc
     4921 cacaccttca tcattagcat taatccgtat gtgtttgcta gcaacatgca cgttgaattc
     4981 agagacatac acagaggacc cctgtggtgc aggtttctta ggcgacccca aaacggccgt
     5041 aaccaggtcc ggctctttct caggctttcc tcaaagaggg acatgtgtag acccccacta
     5101 tccctgacac acactcaatc cggacagtgt ggccggacac aggcaagcaa taaaacctgg
     5161 attagcagag tgttcacaca taacactaga gcgagaaagt ttcttaacac gcccaagccc
     5221 accctctcct cacacacgtc tctcctctct ttcattccct ccatctctcc ttcttttgtg
     5281 tctgcgtctc tcctccctcc tcttcctttg cccctttctc ttcagaattt cattgtttgt
     5341 tattaccatg ttagagataa aggaggtctg aggaattagc ttgttcttac agttgcagag
     5401 aaaatccgct taagtccaca acccccaaat ttgcggtacc cagaagcccc agctctcggg
     5461 gtttatcccc aatgcctatc tttgtggtgg ggggaaaaaa tcattttgta aggtgccaac
     5521 ggggctgaga tggagcagtc cgacccatct ccaggccccc gtcctcgcat catgtcctgg
     5581 aaatgggtgc atttgtataa tatcactgct tttgtcccta ctttgtaagc tggagacaaa
     5641 ggccagacac tcctcaatcc cctccccact catccccccc aaacaaacct ttttcattag
     5701 agccgcaaac actttcccct tcaatagccc tgatattacg catcatcctc tgcatctccg
     5761 caggacccga gcgctctaaa gctcctctct tctctcagga cacagagctc gcttaattaa
     5821 accgcactta agccggacaa taagccttcg cgcaggtctg ggcgagcccg ccgagagtat
     5881 tcatggccgt cgtaaagcga aagccgcaag agagcgagag agagagagag aaagctatac
     5941 gccagatact ttgttcacac aagatgaaga gtcaacagcc acccccacac catttctctc
     6001 ctaataacca cctttagaaa ggctccgtcc tcgcacaatg cttctcggct cccctcctct
     6061 cgctctcaat caccctccag gacaatgcct aaagctccgc agccccccag gaccctcggc
     6121 cccacaacag acacttcttt tattgaagct taaacaagaa tctctagttt tctttcttgc
     6181 ttttactttt acttccttaa tactcaagta caattttaat ggagtacttt tttactttta
     6241 ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt tccctaagta
     6301 cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgct cgaccatatg
     6361 ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc
     6421 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6481 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6541 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7921 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7981 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8041 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8101 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8161 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8221 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8281 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8341 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8401 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8521 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8581 ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     8641 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8701 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8761 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8821 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8881 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8941 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9001 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9061 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     9121 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9181 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     9241 cgacggccag tgaattgtaa tacgactcac tata
//
