LOCUS       Exported                8081 bp ds-DNA     circular SYN 30-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Thorsten Wohland
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..17
                     /label=T7 Promoter
                     /note="T7 Promoter"
     misc_feature    24..40
                     /label=M13 Forward
                     /note="M13 Forward"
     misc_feature    819..1679
                     /label=Amp Resistance
                     /note="Amp Resistance"
     misc_feature    2726..2747
                     /label=CAP binding site
                     /note="CAP binding site"
     misc_feature    2762..2792
                     /label=Lac Promoter
                     /note="Lac Promoter"
     misc_feature    2800..2816
                     /label=Lac operator
                     /note="Lac operator"
     misc_feature    2824..2840
                     /label=M13 Rev
                     /note="M13 Rev"
     misc_feature    2858..2876
                     /label=SP6 Promoter
                     /note="SP6 Promoter"
     misc_feature    3044..3123
                     /label=3' Tol2 sequence
                     /note="3' Tol2 sequence"
     misc_feature    3124..7122
                     /label=4kb Wnt3 promoter
                     /note="4KB Wnt3 promoter in reverse orientation"
     misc_feature    7103..7140
                     /label=Ins_mApp(PMT-spe1)-4kbRP
                     /note="Ins_mApp(PMT-spe1)-4kbRP"
     misc_feature    7103..7122
                     /label=flanking sequence for mApple R primer
                     /note="flanking sequence for mApple R primer"
     misc_feature    7123..7167
                     /label=PMT
                     /note="PMT"
     misc_feature    7168..7173
                     /label=Spe1
                     /note="Spe1"
     misc_feature    7174..7884
                     
                     /note="mApple in reverse orientation"
     misc_feature    7885..7907
                     /label=Flanking vector seqeunce next to 5'Tol2
                     /note=" Flanking vector seqeunce next to 5'Tol2 end"
     misc_feature    7908..8067
                     /label=5' Tol2  sequence
                     /note="5' Tol2  sequence"
     misc_feature    8080..8081
                     /label=T7 Promotercontd..
                     /note="T7 Promotercontd.."
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgagcaga ggtgtaaaaa
     2941 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     3001 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     3061 attgtacttg agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg
     3121 tttcaataaa agaagtgtct gttgtggggc cgagggtcct ggggggctgc ggagctttag
     3181 gcattgtcct ggagggtgat tgagagcgag aggaggggag ccgagaagca ttgtgcgagg
     3241 acggagcctt tctaaaggtg gttattagga gagaaatggt gtgggggtgg ctgttgactc
     3301 ttcatcttgt gtgaacaaag tatctggcgt atagctttct ctctctctct ctcgctctct
     3361 tgcggctttc gctttacgac ggccatgaat actctcggcg ggctcgccca gacctgcgcg
     3421 aaggcttatt gtccggctta agtgcggttt aattaagcga gctctgtgtc ctgagagaag
     3481 agaggagctt tagagcgctc gggtcctgcg gagatgcaga ggatgatgcg taatatcagg
     3541 gctattgaag gggaaagtgt ttgcggctct aatgaaaaag gtttgtttgg gggggatgag
     3601 tggggagggg attgaggagt gtctggcctt tgtctccagc ttacaaagta gggacaaaag
     3661 cagtgatatt atacaaatgc acccatttcc aggacatgat gcgaggacgg gggcctggag
     3721 atgggtcgga ctgctccatc tcagccccgt tggcacctta caaaatgatt ttttcccccc
     3781 accacaaaga taggcattgg ggataaaccc cgagagctgg ggcttctggg taccgcaaat
     3841 ttgggggttg tggacttaag cggattttct ctgcaactgt aagaacaagc taattcctca
     3901 gacctccttt atctctaaca tggtaataac aaacaatgaa attctgaaga gaaaggggca
     3961 aaggaagagg agggaggaga gacgcagaca caaaagaagg agagatggag ggaatgaaag
     4021 agaggagaga cgtgtgtgag gagagggtgg gcttgggcgt gttaagaaac tttctcgctc
     4081 tagtgttatg tgtgaacact ctgctaatcc aggttttatt gcttgcctgt gtccggccac
     4141 actgtccgga ttgagtgtgt gtcagggata gtgggggtct acacatgtcc ctctttgagg
     4201 aaagcctgag aaagagccgg acctggttac ggccgttttg gggtcgccta agaaacctgc
     4261 accacagggg tcctctgtgt atgtctctga attcaacgtg catgttgcta gcaaacacat
     4321 acggattaat gctaatgatg aaggtgtggc tatattaggt acaaaaaaaa aaactttgac
     4381 aatagttgta taaactcctg tcaacagtcc atcaatgtat gaagcagaac tcgctttgaa
     4441 accaaacatt gttgtgtggg tccatttgga ccagtttaag gaaatctttt gccctataaa
     4501 ttccagcctg catgaatttt tatgattatt gctcataaac atacttttaa aaatgtgcat
     4561 tttaactaac tatcaacttg tgcccgtcca ctcaaaggaa gccaaaaggg tgtctctccc
     4621 agatcacagg gactactttg tgggtgtgta gggtgatgta ttagctaggc gctaataaaa
     4681 tgcaggagtg tccacaaaca tgacttaatg agggaggatg agagactcct gacccagagg
     4741 tcaaagctcc gggcgactct ctttattccc attgatctgc tggtgcactt catttccatg
     4801 ctctagggcc cacttcagta aattcattaa gtgcgattga tacgagaatc aagacttcct
     4861 ccaaaacaca atggctatgc taggagggaa tggtcttgtt tgtgctgcag ctatgtcaag
     4921 catctctggc ttttgtcctg gtttcgcacc attgcggtgc atgctggggt ttgagtcctc
     4981 gagattctaa tcgtaagctc atttccagct ggttcgttgt gttgcgcccc tatggccttg
     5041 acctgggaaa cgtggtttca gtgttgttga gatgccgacc gcagaccgtt ccttgcctcc
     5101 actcgtaaat tacgctccga tcaggttttc ccagccaatc ctggcgagca gatctgcatc
     5161 tgtcactctg caagaatgct gaatcaaacc gctgatctag gcacaataca gctgatggaa
     5221 agcagatctt tattccaaca tcaggaacgt agcacgagcc gagtgcttct ggaagctgca
     5281 atttaaccaa caggttcata tctgatgaga tcaaggatgc atcatcctgg aagaaacact
     5341 gtgatgaaca tttaagatgt cagaatgacc cgtcatgcct ctgagctgag tccccgcaca
     5401 gcgcttattc ttttgaagag ggagaataaa agcaaaaaaa cagaggcgat ggaaatatga
     5461 catccagacc gcggtcagaa ctgtgagtgt ctctcctggg ctcctatggt tgtggtgcac
     5521 agtccatgtc cctgctatga gttagataga cagatgcact ggcagataga cagacagaca
     5581 ggctttacta cttggtcgct ggattgcagg aaagagcttt atccaaccag agtcaagcaa
     5641 gcaacatggg acaataacat aattaaatat tctggggaaa agtggcactt atgcaaatgt
     5701 tttttgtaat gggttggaga aaattggccg gaacctacat aatttttaat gacaggatga
     5761 ttcatttgtg aaattgtaat tcaaagaaat tcagtaaact aactaacaat ctaaaaaatt
     5821 ctttgatttg cagtttaatt ctacttccta acattgattt gtttcaactt tattcaaatg
     5881 gcaaaactaa attagtactc aaagctccaa atgatatgat tcctagactg tttaaagtgc
     5941 taaaagtgcc aaaacttaca gctacccaca tgaacttata tttcattctg cctccatttg
     6001 caattcctcc agtgtttgta cctgaaactg ttgtcagttc tcacctctgt cctgccgttc
     6061 attctgacac agagactcta tatttgaatg tattgtcaac ttctaatgcc gtgtctacta
     6121 ctctgtgctg tctgctctca tcccatccga atgacaatag tgtttatgta aaatgtgctg
     6181 cctttgggcc gctggggcac agaacactgc atgcctggcc ctgccacacc accttttacg
     6241 gctttttatt gtgttgcctt tgtgtcacaa ggggaggacg cggccatcaa aattctccaa
     6301 gacaaaattc ttgaatgtat gtgcacgcac acgcatccgg aacaattagt tcaggagcac
     6361 gggtgcttaa aaaacggaga cgacacttct cgccaccacc tcctcgccga gcagcaggcc
     6421 cgagtctggc acagctgggt ttccttgatg ctaacgcaga caatatagat cttaataaga
     6481 caaatctacc tctccgaaac tcagtcctga caccctttca ttcttattcc ctcccagcct
     6541 cctctctctc tctctctctc tttctttctt tctttgctac attaccaggg ggtggtgcat
     6601 gattgacaga tgggcaggaa taataatttt tttttttttt ttgcgtaacc taaggccatg
     6661 ctctgtgatt ggcttgccag ctgacatctc caaacctctc ttgtcgtttt tttcccctct
     6721 ctctctctcg ctttctctct ctcactctct ctctctaata ttggccctga tgatcctaag
     6781 gggaaaaaag tggggagaga aaaagatatg taccttaggg aagaacctca gtaagaggga
     6841 gggaatcaga ggacgagtag gagcgtgtgt gcgagtgtgt gaaagagaca cttactctcc
     6901 taactactac tggaagacac acacttttct cacccaactc aaagcagcag caaaccttgg
     6961 agaattgttt tatttttgtt ttttcgcgaa ccccccaaaa aaattcctaa ttattctctc
     7021 aagcatccat ctttgccttg ccgggattta attttcaccc cctctctagg aatcctatgt
     7081 actgatcaat agaccctttc catccacaca agacatacct cgatgggctg cttcttcagc
     7141 aagcggcgga aggccgacaa ggagagcact agtatggtga gcaagggcga ggagaataac
     7201 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     7261 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
     7321 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     7381 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
     7441 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
     7501 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
     7561 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     7621 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
     7681 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
     7741 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
     7801 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     7861 ggcatggacg agctgtacaa gtaagctcta gatggccaga tctatttaaa ttgtaaggag
     7921 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     7981 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     8041 aaattactca agtactttac acctctgggc ccaattcgcc c
//
