LOCUS       Exported               14917 bp ds-DNA     circular SYN 03-JUN-2023
DEFINITION  Bacterial vector with a CloDF13 (CDF) origin for the co-expression 
            of two genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     source          1469..1568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1469..1568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1469..1568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1469..1568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1469..1568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1469..1568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1475..1502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1475..1502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1503..1534
                     /organism="Corynebacterium glutamicum MB001"
                     /strain="MB001"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1310161"
     source          1535..1562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1535..1562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1535..1562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1535..1562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1614..1636
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1614..1636
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6377..6399
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6377..6399
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(11725..14917,1..11724)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(11725..14917,1..11724)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(304..927)
                     /codon_start=1
                     /label=tetR
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    1318..1468
                     /label=tet promoter
     misc_feature    1475..1502
                     /label=repeat
     gene            complement(1503..1534)
                     /gene="crtYf"
                     /locus_tag="cgp_0718"
                     /label=crtYf
     misc_feature    1503..1534
                     /label=crRNA
     misc_feature    1535..1562
                     /label=repeat
     misc_feature    1569..1613
                     /label=MCS
     misc_feature    1614..1636
                     /label=tet promoter Range
     CDS             1637..2821
                     /codon_start=1
                     /label=TniQ
                     /translation="MFLQRPKPYSDESLESFFIRVANKNGYGDVHRFLEATKRFLQDID
                     HNGYQTFPTDITRINPYSAKNSSSARTASFLKLAQLTFNEPPELLGLAINRTNMKYSPS
                     TSAVVRGAEVFPRSLLRTHSIPCCPLCLRENGYASYLWHFQGYEYCHSHNVPLITTCSC
                     GKEFDYRVSGLKGICCKCKEPITLTSRENGHEAACTVSNWLAGHESKPLPNLPKSYRWG
                     LVHWWMGIKDSEFDHFSFVQFFSNWPRSFHSIIEDEVEFNLEHAVVSTSELRLKDLLGR
                     LFFGSIRLPERNLQHNIILGELLCYLENRLWQDKGLIANLKMNALEATVMLNCSLDQIA
                     SMVEQRILKPNRKSKPNSPLDVTDYLFHFGDIFCLWLAEFQSDEFNRSFYVSRW"
     CDS             2822..4744
                     /codon_start=1
                     /label=Cas8
                     /translation="MQTLKELIASNPDDLTTELKRAFRPLTPHIAIDGNELDALTILVN
                     LTDKTDDQKDLLDRAKCKQKLRDEKWWASCINCVNYRQSHNPKFPDIRSEGVIRTQALG
                     ELPSFLLSSSKIPPYHWSYSHDSKYVNKSAFLTNEFCWDGEISCLGELLKDADHPLWNT
                     LKKLGCSQKTCKAMAKQLADITLTTINVTLAPNYLTQISLPDSDTSYISLSPVASLSMQ
                     SHFHQRLQDENRHSAITRFSRTTNMGVTAMTCGGAFRMLKSGAKFSSPPHHRLNSKRSW
                     LTSEHVQSLKQYQRLNKSLIPENSRIALRRKYKIELQNMVRSWFAMQDHTLDSNILIQH
                     LNHDLSYLGATKRFAYDPAMTKLFTELLKRELSNSINNGEQHTNGSFLVLPNIRVCGAT
                     ALSSPVTVGIPSLTAFFGFVHAFERNINRTTSSFRVESFAICVHQLHVEKRGLTAEFVE
                     KGDGTISAPATRDDWQCDVVFSLILNTNFAQHIDQDTLVTSLPKRLARGSAKIAIDDFK
                     HINSFSTLETAIESLPIEAGRWLSLYAQSNNNLSDLLAAMTEDHQLMASCVGYHLLEEP
                     KDKPNSLRGYKHAIAECIIGLINSITFSSETDPNTIFWSLKNYQNYLVVQPRSINDETT
                     DKSSL"
     CDS             4716..5774
                     /codon_start=1
                     /label=Cas7
                     /translation="MKLPTNLAYERSIDPSDVCFFVVWPDDRKTPLTYNSRTLLGQMEA
                     ASLAYDVSGQPIKSATAEALAQGNPHQVDFCHVPYGASHIECSFSVSFSSELRQPYKCN
                     SSKVKQTLVQLVELYETKIGWTELATRYLMNICNGKWLWKNTRKAYCWNIVLTPWPWNG
                     EKVGFEDIRTNYTSRQDFKNNKNWSAIVEMIKTAFSSTDGLAIFEVRATLHLPTNAMVR
                     PSQVFTEKESGSKSKSKTQNSRVFQSTTIDGERSPILGAFKTGAAIATIDDWYPEATEP
                     LRVGRFGVHREDVTCYRHPSTGKDFFSILQQAEHYIEVLSANKTPAQETINDMHFLMAN
                     LIKGGMFQHKGD"
     CDS             6008..6376
                     /codon_start=1
                     /label=Cas6
                     /translation="MEQLQYFHISNTVLVPEDCTYVSFRRCQSIDKLTAAGLARKIRRL
                     EKRALSRGEQFDPSSFAQKEHTAIAHYHSLGESSKQTNRNFRLNIRMLSEQPREGNSIF
                     SSYGLSNSENSFQPVPLI"
     misc_feature    6377..6399
                     /label=ptet
                     /note="/vntifkey=21"
     CDS             6400..7092
                     /codon_start=1
                     /label=TnsA
                     /translation="MATSLPTPSAITTSALEYAFHTPARNLTKSRGKNIHRYVSVKMSK
                     RITVESTLECDACYHFDFEPSIVRFCAQPIRFLYYLNGQSHSYVPDFLVQFDTNEFVLY
                     EVKSAYAKNKPDFDVEWEAKVKAATELGLELELVEESDIRDTVVLNNLKRMHRYASKDE
                     LNNVHNSLLKIIKYNGAQSARCLGEQLGLKGRTVLPILCDLLSRCLLDTRLDKPLSLES
                     RFELASYG"
     misc_feature    7085..8896
                     /label=TnsB
     CDS             8908..9900
                     /codon_start=1
                     /label=TnsC
                     /translation="MSETREARISRAKRAFVSTPSVRKILSYMDRCRDLSDLESEPTCM
                     MVYGASGVGKTTVIKKYLNQNRRESEAGGDIIPVLHIELPDNAKPVDAARELLVEMGDP
                     LALYETDLARLTKRLTELIPAVGVKLIIIDEFQHLVEERSNRVLTQVGNWLKMILNKTK
                     CPIVIFGMPYSKVVLQANSQLHGRFSIQVELRPFSYQGGRGVFKTFLEYLDKALPFEKQ
                     AGLANESLQKKLYAFSQGNMRSLRNLIYQASIEAIDNQHETITEEDFVFASKLTSGDKP
                     NSWKNPFEEGVEVTEDMLRPPPKDIGWEDYLRHSTPRVSKPGRNKNFFE"
     terminator      9930..9977
                     /label=T7 terminator
     CDS             complement(10217..11011)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    11378..13922
                     /label=pBL1ts
     rep_origin      complement(13970..14708)
                     /direction=LEFT
                     /label=CloDF13 ori
ORIGIN
        1 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
       61 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
      121 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      181 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttaaattct agactgatga
      241 atcccctaat gatttttatc aaaatcatta aggttaccat cacggaaaaa ggttatgctg
      301 cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      481 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      541 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      601 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      661 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      721 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      781 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
      841 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
      901 cactttactt ttatctaatc tagacatgtt gtgtctcctc taaagattgt aggaaatgca
      961 atgtgtcaag cagttaggtc aatcagagca tcggtcactc aagaattctt gagctgacag
     1021 aacccaaggg cgagtatggc gaatttttcc aactcacgca ccaaacctac tcacattgga
     1081 taattcgtcg agtcaactcc gtgtcaacct gtcctcaact ttagcgggga aacaaagatt
     1141 tcgcagcaga tgcaaaatca tacagacaca ttcaaaaatg gacattgacc ccaaacgggg
     1201 gcatttccaa cacttgcgct aaatagtttt tgcagggaac gaacctgaaa tcgttcgcac
     1261 tacgatctgc tcccccgaat ggggttaatt atgaactaca ttacaccccc gtgaatcgaa
     1321 ttccaaaaac taaaaaaaat attgacactc tatcattgat agagtataat taacgggatc
     1381 cactcttcat tgataagtcg ccaagcttgg gatccccagc ttgttgatac actaatgctt
     1441 ttatataggg aaaaggtggt gaactactcc atgggtgaac tgccgagtag gtagctgata
     1501 acaggcaacc atagggcagg aatcagaagt actggtgaac tgccgagtag gtagctgata
     1561 acggatccga attcgagctc ggcgcgcctg caggtcgaca agcttgcggc cgctagggaa
     1621 aaggtggtga actactatgt tcctgcagcg cccaaagcca tactccgatg agtccctcga
     1681 atccttcttc atccgcgtgg ctaacaagaa cggctacggc gacgtccacc gcttcctgga
     1741 ggcaaccaag cgcttcctcc aggatatcga ccacaacggc taccagacct tcccaaccga
     1801 tatcacccgc atcaacccat actccgccaa gaactcctcc tccgctcgca ccgcatcctt
     1861 cctgaagctc gcccagctga ccttcaacga gccaccagaa ctgctcggcc tcgctatcaa
     1921 ccgcaccaac atgaagtact ccccatccac ctccgcagtg gtccgtggtg cagaagtgtt
     1981 cccacgttcc ctgctccgca cccactccat cccatgctgc ccactgtgcc tccgcgagaa
     2041 cggctacgct tcctacctgt ggcacttcca gggctacgaa tactgccact cccacaacgt
     2101 gccactcatc accacctgct cctgcggcaa ggagttcgac taccgcgtct ccggcctgaa
     2161 gggcatctgc tgcaagtgca aggaaccaat caccctcacc tcccgcgaga acggccacga
     2221 agcagcctgc accgtgtcca actggctggc tggccacgag tccaagccac tgccaaacct
     2281 cccaaagtcc taccgttggg gtctggtgca ctggtggatg ggcatcaagg attccgaatt
     2341 cgaccacttc tccttcgtcc agttcttctc caactggcca cgctccttcc actccatcat
     2401 cgaagatgag gtcgaattca acctggagca cgcagtggtc tccacctccg aactgcgtct
     2461 caaggacctg ctcggtcgtc tgttcttcgg ctccatccgc ctgccagaac gcaacctcca
     2521 gcacaacatc atcctcggcg agctgctctg ctacctggaa aaccgcctct ggcaggataa
     2581 gggcctgatc gcaaacctca agatgaacgc cctggaggct accgtgatgc tgaactgctc
     2641 cctcgaccag atcgcctcca tggtcgaaca gcgcatcctg aagccaaacc gcaagtccaa
     2701 gccaaactcc ccactggatg tgaccgacta cctcttccac ttcggcgata tcttctgcct
     2761 gtggctcgca gagttccagt ccgacgaatt caaccgctcc ttctacgtct cgaggtggta
     2821 aatgcagacc ctgaaggagc tcatcgcctc caacccagat gacctgacca ccgaactcaa
     2881 gcgcgctttc cgcccactga ccccacacat cgcaatcgat ggcaacgagc tggacgccct
     2941 caccatcctg gtgaacctca ccgataagac cgatgaccag aaggatctgc tcgaccgcgc
     3001 aaagtgcaag cagaagctgc gcgacgaaaa gtggtgggcc tcctgcatca actgcgtgaa
     3061 ctaccgccag tcccacaacc caaagttccc agatatccgt tccgagggtg tcatccgtac
     3121 ccaggcactg ggcgaactcc catccttcct gctctcctcc tccaagatcc caccatacca
     3181 ctggtcctac tcccacgatt ccaagtacgt caacaagtcc gccttcctga ccaacgaatt
     3241 ctgctgggac ggcgagatct cctgcctggg tgaactgctc aaggatgctg accacccact
     3301 ctggaacacc ctgaagaagc tcggctgctc ccagaagacc tgcaaggcta tggcaaagca
     3361 gctcgctgat atcaccctga ccaccatcaa cgtgaccctc gcaccaaact acctgaccca
     3421 gatctccctc ccagattccg acacctccta tatctccctg tccccagtcg cttccctctc
     3481 catgcagtcc cacttccacc agcgcctgca ggatgaaaac cgccactccg caatcacccg
     3541 cttctcccgc accaccaaca tgggtgtgac cgctatgacc tgcggtggtg cattccgcat
     3601 gctgaagtcc ggcgcaaagt tctcctcccc accacaccac cgcctgaact ccaagcgctc
     3661 ctggctcacc tccgaacacg tgcagtccct gaagcagtac cagcgcctga acaagtccct
     3721 catcccagag aactcccgca tcgcactgcg ccgcaagtac aagatcgaac tccagaacat
     3781 ggtccgctcc tggttcgcca tgcaggatca caccctggac tccaacatcc tgatccagca
     3841 cctcaaccac gatctgtcct acctcggcgc caccaagcgc ttcgcatacg acccagccat
     3901 gaccaagctg ttcaccgaac tgctcaagcg cgagctctcc aactccatca acaacggcga
     3961 gcagcacacc aacggctcct tcctggtgct cccaaacatc cgtgtctgcg gtgcaaccgc
     4021 tctgtcctcc ccagtgaccg tcggcatccc atccctcacc gccttcttcg gcttcgtgca
     4081 cgctttcgaa cgcaacatca accgcaccac ctcctccttc cgcgtcgagt ccttcgctat
     4141 ctgcgtgcac cagctgcacg tcgaaaagcg cggcctcacc gcagaattcg tggagaaggg
     4201 cgatggcacc atctccgcac cagccacccg cgatgactgg cagtgcgacg tggtcttctc
     4261 cctgatcctc aacaccaact tcgcccagca catcgatcag gacaccctgg tcacctccct
     4321 cccaaagcgt ctggctcgtg gttccgcaaa gatcgctatc gatgacttca agcacatcaa
     4381 ctccttctcc accctggaaa ccgctatcga gtccctccca atcgaggcag gtcgttggct
     4441 gtccctctac gcacagtcca acaacaacct gtccgatctg ctcgcagcca tgaccgaaga
     4501 ccaccagctc atggcatcct gcgtgggcta ccacctgctc gaagagccaa aggataagcc
     4561 aaactccctg cgcggctaca agcacgctat cgcagagtgc atcatcggcc tcatcaactc
     4621 catcaccttc tcctccgaaa ccgacccaaa caccatcttc tggtccctga agaactacca
     4681 gaactacctc gtggtccagc caaggagtat caacgatgaa actaccgaca aatctagcct
     4741 atgagcgctc catcgatcca tccgacgtct gcttcttcgt ggtctggcca gatgaccgca
     4801 agaccccact cacctacaac tcccgcaccc tgctcggcca gatggaagca gcctccctgg
     4861 cttacgatgt gtccggccag ccaatcaagt ccgcaaccgc tgaggcactc gcacagggta
     4921 acccacacca ggtggacttc tgccacgtcc catacggcgc atcccacatc gaatgctcct
     4981 tctccgtctc cttctcctcc gagctgcgcc agccatacaa gtgcaactcc tccaaggtga
     5041 agcagaccct ggtgcagctc gtcgaactgt acgagaccaa gatcggctgg accgaactcg
     5101 ccacccgcta cctgatgaac atctgcaacg gcaagtggct ctggaagaac acccgcaagg
     5161 cttactgctg gaacatcgtc ctgaccccat ggccatggaa cggcgaaaaa gtgggcttcg
     5221 aggatatccg caccaactac acctcccgcc aggacttcaa gaacaacaag aactggtccg
     5281 caatcgtcga aatgatcaag accgccttct cctccaccga tggcctggcc atcttcgagg
     5341 tgcgcgctac cctgcacctc ccaaccaacg ccatggtgcg cccatcccag gtcttcaccg
     5401 aaaaggagtc cggctccaag tccaagtcca agacccagaa ctcccgcgtc ttccagtcca
     5461 ccaccatcga cggtgaacgt tccccaatcc tcggtgcttt caagaccggc gctgcaatcg
     5521 caaccatcga tgactggtac ccagaagcaa ccgagccact gcgtgtgggt cgtttcggtg
     5581 tccaccgcga agatgtgacc tgctaccgcc acccatccac cggcaaggac ttcttctcca
     5641 tcctccagca ggcagaacac tacatcgagg tgctgtccgc taacaagacc ccagcacagg
     5701 agaccatcaa cgatatgcac ttcctcatgg ccaacctgat caagggcggc atgttccagc
     5761 acaaaggaga ctgactgtga agtggtacta caagaccatc accttcctcc cagaactgtg
     5821 caacaacgag tccctcgcag caaagtgcct ccgtgtgctg cacggcttca actaccagta
     5881 cgaaacccgc aacatcggcg tctccttccc actctggtgc gatgctaccg tgggcaagaa
     5941 gatctccttc gtctccaaga acaagatcga actggacctg ctcctgaagc agcactactt
     6001 cgtgcagatg gagcagctcc agtacttcca catctccaac accgtgctgg tcccagagga
     6061 ttgcacctac gtctccttcc gccgctgcca gtccatcgac aagctcaccg ctgcaggtct
     6121 ggctcgtaag atccgtcgtc tcgaaaagcg tgcactgtcc cgtggcgagc agttcgatcc
     6181 atcctccttc gcacagaagg aacacaccgc catcgctcac taccactccc tgggcgagtc
     6241 ctccaagcag accaaccgca acttccgcct caacatccgc atgctgtccg aacagccacg
     6301 cgagggcaac tccatcttct cctcctacgg cctctccaac tccgaaaact ccttccagcc
     6361 agtgccactg atctaatagg gaaaaggtgg tgaactacta tggcaacctc cctgccaacc
     6421 ccatccgcaa tcaccacctc cgccctggaa tacgcattcc acaccccagc acgcaacctg
     6481 accaagtccc gcggcaagaa catccaccgc tacgtgtccg tgaagatgtc caagcgcatc
     6541 accgtggaat ccaccctgga atgcgatgca tgctaccact tcgacttcga accatccatc
     6601 gtgcgcttct gcgcacagcc aatccgcttc ctgtactacc tgaacggcca gtcccactcc
     6661 tacgtgccag acttcctggt gcagttcgac accaacgaat ttgtgctgta cgaagtgaag
     6721 tccgcatacg caaagaacaa gccagacttc gatgtggaat gggaagcaaa ggtgaaggcc
     6781 gcaaccgaac tgggcctgga actggaactg gtggaagaat ccgacatccg cgacaccgtg
     6841 gtgctgaaca acctgaagcg catgcaccgc tacgcctcca aggacgaact gaacaacgtg
     6901 cacaactccc tgctgaagat catcaagtac aacggcgcac agtccgcacg ctgcctgggc
     6961 gaacagctgg gcctgaaggg ccgcaccgtg ctgccaatcc tgtgcgacct gctgtcccgc
     7021 tgcctgctgg atacccgcct ggataagcca ctgtccctgg aatcccgctt cgagttggcc
     7081 agttatggct aagaagggct tctcctcctt ccaccgcaag gctgtgtcct cccaggatac
     7141 cctcgaatcc atcgagctgg tgtcctccgc aaactgcctc gaatccgtca cctaccagga
     7201 catctccgct ttcccagaga ccatcgcagt cgaaatcaac ttccgcctgt ccatcctccg
     7261 cttcctggca cgcaagtgcg aaaccatcgt ggccaagtcc atcgagccac accgcgtcga
     7321 actgcagcag aactactccc gcaagatccc atccgccatc accatctacc gctggtggct
     7381 cgctttccgc aagtccgatt acaacccaat ctccctggcc ccaaacatca aggaccgcgg
     7441 caaccgcgaa accaaggtgt ccaccgtggt cgattccatc atggagcagg cagtggaacg
     7501 cgtcatctcc ggccgcaagg tgaacgtctc ctccgcatac aagcgtgtgc gtcgtaaggt
     7561 ccgccagtac aacctgaccc acggcaccaa gtacacctac ccaaagtacg agtccgtgcg
     7621 caagcgcgtg aagaagaaga ccccattcga actgctcgca gcaggcaagg gcgagcgtgt
     7681 ggctaagcgc gaattccgcc gcatgggcaa gaagatcctc acctcctccg tgctggaacg
     7741 cgtcgagatc gatcacaccg tggtcgacct cttcgcagtc cacgaagagt accgtatccc
     7801 actgggtcgt ccatggctca cccagctggt ggattgctac tccaaggccg tcatcggctt
     7861 ctacctgggc ttcgagccac catcctacgt gtccgtctcc ctggcactca agaacgccat
     7921 ccagcgcaag gatgacctca tctcctccta cgaatccatc gaaaacgagt ggctgtgcta
     7981 cggcatccca gatctgctcg tgaccgacaa cggcaaggag ttcctgtcca aggctttcga
     8041 tcaggcatgc gaatccctgc tcatcaacgt gcaccagaac aaggtcgaga ccccagacaa
     8101 caagccacac gtggaacgca actacggcac catcaacacc tccctgctcg atgacctccc
     8161 tggcaagtcc ttctcccagt acctgcagcg cgagggctac gattccgtcg gcgaagcaac
     8221 cctgaccctc aacgaaatcc gcgagatcta cctcatctgg ctggtggaca tctaccacaa
     8281 gaagccaaac cagcgcggca ccaactgccc aaacgtcgct tggaagaagg gctgccagga
     8341 atgggagcca gaagagttct ccggctccaa ggatgaactc gacttcaagt tcgcaatcgt
     8401 ggattacaag cagctgacca aagtgggcat caccgtctac aaggagctct cctactccaa
     8461 cgaccgcctg gccgaatacc gcggcaagaa gggcaaccac aaggtgcagt tcaagtacaa
     8521 cccagagtgc atggctgtga tctgggtcct ggatgaggac atgaacgaat acttcaccgt
     8581 gaacgccatc gattacgaat acgcttcccg cgtctccctc tggcagcaca agtacaacat
     8641 gaagtaccag gccgagctga actccgctga atacgatgag gacaaggaaa tcgatgctga
     8701 aatcaagatc gaagagatcg cagaccgctc catcgtgaag accaacaaga tccgtgcacg
     8761 tcgtcgtggt gcacgtcacc aggaaaactc cgctcgcgca aagtccatct ccaacgccaa
     8821 cccagcttcc atccagaagc acgaagatga gatcgtgtcc gccgacaacg atgactggga
     8881 tatcgactac gtctgagaaa tcgtcaaatg tccgaaaccc gtgaggctcg tatctcccgt
     8941 gctaagcgcg cattcgtgtc caccccatcc gtccgcaaga tcctctccta catggatcgc
     9001 tgccgcgatc tgtccgacct cgaatccgag ccaacctgca tgatggtgta cggtgcatcc
     9061 ggtgtgggca agaccaccgt catcaagaag tacctgaacc agaaccgtcg tgaatccgag
     9121 gcaggtggtg acatcatccc agtcctgcac atcgaactcc cagataacgc taagccagtg
     9181 gacgcagccc gcgaactgct cgtcgagatg ggcgatccac tggctctcta cgaaaccgac
     9241 ctggcacgcc tcaccaagcg tctgaccgag ctcatcccag cagtgggcgt caagctgatc
     9301 atcatcgatg aattccagca cctcgtggaa gagcgttcca accgtgtgct gacccaggtc
     9361 ggcaactggc tgaagatgat cctcaacaag accaagtgcc caatcgtcat cttcggcatg
     9421 ccatactcca aggtggtcct gcaggctaac tcccagctcc acggccgctt ctccatccag
     9481 gtggaactgc gtccattctc ctaccagggt ggtcgtggcg tcttcaagac cttcctggag
     9541 tacctcgaca aggcactgcc attcgaaaag caggccggcc tcgctaacga gtccctgcag
     9601 aagaagctct acgccttctc ccagggcaac atgcgctccc tgcgcaacct catctaccag
     9661 gcatccatcg aagccatcga taaccagcac gagaccatca ccgaagagga cttcgtgttc
     9721 gcatccaagc tgacctccgg cgataagcca aactcctgga agaacccatt cgaagagggc
     9781 gtggaagtca ccgaggacat gctccgccca ccaccaaagg atatcggctg ggaagactac
     9841 ctgcgtcact ccaccccacg tgtgtccaag ccaggtcgca acaagaactt cttcgagtaa
     9901 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     9961 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
    10021 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
    10081 ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg gcacttttcg gggaaatgtg
    10141 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat
    10201 taattcaagc ttcatatcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
    10261 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
    10321 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
    10381 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
    10441 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga
    10501 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
    10561 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
    10621 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
    10681 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
    10741 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
    10801 ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg
    10861 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
    10921 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
    10981 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
    11041 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
    11101 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
    11161 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
    11221 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
    11281 agcaccgttt ctgcggactg gctttctacg tgttccgctt ccggtgaccg cctcgatgat
    11341 cgccgggtgg gcgtggccca aggaggatca tccagccatt cggggtcgtt cactggttcc
    11401 cctttctgat ttctggcata gaagaacccc cgtgaactgt gtggttccgg gggttgctga
    11461 tttttgcgag acttctcgcg caattcccta gcttaggtga aaacaccatg aaacactagg
    11521 gaaacaccca tgaaacaccc attagggcag tagggcggct tcttcgtcta gggcttgcat
    11581 ttgggcggtg atctggtctt tagcgtgtga aagtgtgtcg taggtggcgt gctcaatgca
    11641 ctcgaacgtc acgtcattta ccgggtcacg gtgggcaaag agaactagtg ggttagacat
    11701 tgttttcctc gttgtcggtg gtggtgagct tttctagccg ctcggtaaac gcggcgatca
    11761 tgaactcttg gaggttttca ccgttctgca tgcctgcgcg cttcatgtcc tcacgtagtg
    11821 ccaaaggaac gcgtgcggtg accacgacgg gcttagcctt tgcctgcgct tctagtgctt
    11881 cgatggtggc ttgtgcctgc gcttgctgcg cctgtagtgc ctgttgagct tcttgtagtt
    11941 gctgttctag ctgtgccttg gttgccatgc tttaagactc tagtagcttt cctgcgatat
    12001 gtcatgcgca tgcgtagcaa acattgtcct gcaactcatt cattatgtgc agtgctcctg
    12061 ttactagtcg tacatactca tatttaccta gtctgcatgc agtgcatgca catgcagtca
    12121 tgtcgtgcta atgtgtaaaa catgtacatg cagattgctg ggggtgcagg gggcggagcc
    12181 accctgtcca tgcggggtgt ggggcttgcc ccgccggtac agacagtgag caccggggca
    12241 cctagtcgcg gatacccccc ctaggtatcg gacacgtaac cctcccatgt cgatgcaaat
    12301 ctttaacatt gagtacgggt aagctggcac gcatagccaa gctaggcggc caccaaacac
    12361 cactaaaaat taatagtccc tagacaagac aaacccccgt gcgagctacc aactcatatg
    12421 cacgggggcc acataacccg aaggggtttc aattgacaac catagcacta gctaagacaa
    12481 cgggcacaac acccgcacaa actcgcactg cgcaaccccg cacaacatcg ggtctaggta
    12541 acactgaaat agaagtgaac acctctaagg aaccgcaggt caatgagggt tctaaggtca
    12601 ctcgcgctag ggcgtggcgt aggcaaaacg tcatgtacaa gatcaccaat agtaaggctc
    12661 tggcggggtg ccataggtgg cgcagggacg aagctgttgc ggtgtcctgg tcgtctaacg
    12721 gtgcttcgca gtttgagggt ctgcaaaact ctcactctcg ctgggggtca tctctggctg
    12781 aattggaagt catgggcgaa cgccgcattg agctggctat tgctactaag aatcacttgg
    12841 cggcgggtgg cgcgctcatg atgtttgtgg gcactgttcg acacaaccgc tcacagtcat
    12901 ttgcgcaggt tgaagcgggt attaagactg cgtactcttc gatggtgaaa acatctcagt
    12961 ggaagaaaga acgtgcacgg tacggggtgg agcacaccta tagtgactat gaggtcacag
    13021 actcttgggc gaacggttgg cacttgcacc gcaacatgct gttgttcttg gatcgtccac
    13081 tgtctgacga tgaactcaag gcgtttgagg attccatgtt ttcccgctgg tctgctggtg
    13141 tggttaaggc cggtatggac gcgccactgc gtgagcacgg ggtcaaactt gatcaggtgt
    13201 ctacctgggg tggagacgct gcgaaaatgg caacctacct cgctaagggc atgtctcagg
    13261 aactgactgg ctccgctact aaaaccgcgt ctaaggggtc gtacacgccg tttcagatgt
    13321 tggatatgtt ggccgatcaa agcgacgccg gcgaggatat ggacgctgtt ttggtggctc
    13381 ggtggcgtga gtatgaggtt ggttctaaaa acctgcgttc gtcctggtca cgtggggcta
    13441 agcgtgcttt gggcattgat tacatagacg ctgatgtacg tcgtgaaatg gaagaagaac
    13501 tgtacaagct cgccggtctg gaagcaccgg aacgggtcga atcaacccgc gttgctgttg
    13561 ctttggtgaa gcccgatgat tggaaactga ttcagtctga tttcgcggtt aggcagtacg
    13621 ttctcgattg cgtggataag gctaaggacg tggccgctgc gcaacgtgtc gctaatgagg
    13681 tgctggcaag tctgggtgtg gattccaccc cgtgcatgat cgttatggat gatgtggact
    13741 tggacgcggt tctgcctact catggggacg ctactaagcg tgatctgaat gcggcggtgt
    13801 tcgcgggtaa tgagcagact attcttcgca cccactaaaa gcggcataaa ccccgttcga
    13861 tattttgtgc gatgaattta tggtcaatgt cgcgggggca aactatgatg ggtcttgttg
    13921 tttagctagc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac
    13981 acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac
    14041 agcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata
    14101 aacagacgct tttccggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt
    14161 acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggcgggtc gctccctctt
    14221 gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt ctcccttacg
    14281 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
    14341 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
    14401 gtaactgttc acttgagtcc aacccggaaa agcacggtaa aacgccactg gcagcagcca
    14461 ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg
    14521 tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca
    14581 gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg
    14641 tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc
    14701 ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct tcaaatgtag
    14761 cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc
    14821 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
    14881 ctaatgagtg agctaactta cattaattgc gttgcgc
//
