LOCUS       pGL4.11_Nued2_E1        8155 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pGL4.11_Nued2_E1.gb
FEATURES             Location/Qualifiers
     primer          complement(26..45)
                     /label="OR_pGL4.24_RVseq-3"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="sequence: gttaacgtagccgctcatga"
     primer          378..397
                     /label="OR_pGL4.24_RVseq-6"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="sequence: tggtgttgtgttcgtggacg"
     primer          complement(485..504)
                     /label="OR_pGL4.24_RVseq-5"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: agggaagccgtgagaattca"
     CDS             490..609
                     /label="hPEST"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    661..782
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          804..824
                     /label="OR_pGL4.24-1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="sequence: tttatgtttcaggttcagggg"
     primer          complement(952..971)
                     /label="OR_pGL4.24-2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="sequence: ataagtgcggcgacgatagt"
     rep_origin      complement(1201..1789)
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(1989..2780)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(2781..2849)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     polyA_signal    2954..3002
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3016..3107
                     /label="pause site"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          3056..3075
                     /label="Promega_RVprimer3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: ctagcaaaataggctgtccc"
     primer          3775..3794
                     /label="OR_pGL4.11_Nued2-1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: taattcaggcctagctctgg"
     primer          complement(3858..3877)
                     /label="OR_pGL4.11_Nued-Gibson-RV"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="sequence: gtgtgagacactgtttccag"
     misc_feature    3880..3881
                     /label="Multiple cloning site for enhancers"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     PCR_primer      3888..3907
                     /label="138795315 IS_E18-3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     PCR_primer      3888..3907
                     /label="139204309 IS_E18-7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3888..3907
                     /label="OR_mIgf1_E3_gibson_5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: ttcactctacatcccaccca"
     PCR_primer      3904..3922
                     /label="138795316 IS_E18-4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3953..3976)
                     /label="74471436 OR_mIgf1_E2-16"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="sequence: tggatacctttgctggggctaggg"
     misc_feature    4059..4066
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     variation       4071..4077
                     /label="AP-1 BS, Var2-1 and Var1-1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4087..4109
                     /label="SM756.Igf1.g1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4098..4104
                     /label="AP-1 BS, canonical"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4101..4123
                     /label="SM756.Igf1.g2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4195..4202
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4223..4230
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4260..4282
                     /label="SM757.Igf1.g1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4271..4293)
                     /label="SM757.Igf1.g5"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4283..4289
                     /label="AP-1 BS, canonical"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          4338..4360
                     /label="74471437 OR_mIgf1_E2-17"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: gcctgctgctaccctattcctga"
     primer          complement(4520..4539)
                     /label="OR_mIgf1_E3_gibson_6"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: cctcagtggtttggtctgtt"
     PCR_primer      complement(4520..4539)
                     /label="138795317 IS_E18-5"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4542..4547
                     /label="Multiple cloning site for enhancers"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4628..4648)
                     /label="OR_pGL4.11_Nued2-2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="sequence: gaggacttatgctatttgcag"
     primer          6957..6977
                     /label="OR_pGL4.11-Gibson-FW"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: aagcttggcaatccggtactg"
     regulatory      6986..6995
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             6992..486
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer          complement(7007..7026)
                     /label="OR_pGL4.11-1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: gctgggcccttcttaatgtt"
     primer          7474..7493
                     /label="OR_pGL4.24_RVseq-2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ccaaagcatgtacaccttcg"
     primer          complement(7578..7597)
                     /label="OR_pGL4.24_RVseq-1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: ggtactgccactactgttca"
     primer          8020..8039
                     /label="OR_pGL4.24_RVseq-4"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: agaaacaaccagcgccattc"
ORIGIN
        1 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
       61 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
      121 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
      181 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
      241 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
      301 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
      361 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
      421 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
      481 gccgtgaatt ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
      541 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
      601 atcaacgtct aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
      661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      841 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
      901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
      961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     1981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     2041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     2101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     2161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     2221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     2281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     2341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     2401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     2461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     2521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     2581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     2641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     2701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     2761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     2821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     2941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     3001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     3061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc
     3121 ggtacctgag ctcgctagct tcctggcttg tagtgacctc gtctattgct agaaatcaga
     3181 aggtaccact gagccttcat ttgtttggtg aaatcatgga gttacattaa caagccgttt
     3241 ccccaaacta tggctctcta cccacttgta atacacccgt gcacaggaac attttctgaa
     3301 agtcaatgtt tcctatcact tgctccaaaa ttaaaccaga gatacatcat tcaggaagaa
     3361 gccagatgca ctgaagaaag aaaaacctgc tttatcagaa ggggcaagct tctctcacat
     3421 cccatagcaa tttaaattca tttgccggaa tgttaaatac aaccagtctt ttagttgggt
     3481 cccttcacat aaacaccaag ttagcataaa tgcaaaatag ttcacatgat taaatcttta
     3541 taacaccata atttatctta tgtacacaca cacacacaga gaaagagaga gagagagaga
     3601 gagagagaga tcttttactt gctgatactt ctacattttt tctggagttc tcaactctca
     3661 tcatctctct aaggaagctg gcttgtattg ccccagactc ttagtgtatt tagttctaaa
     3721 ggatagtata cagaataaag ggaaatttta attttgtttt taataggacc tatataattc
     3781 aggcctagct ctggtggaga gaaggggagg agaggtccat tagcatttat tgtgcacctg
     3841 ctttatgcta aacatgtctg gaaacagtgt ctcacacaac cTGCAGGttc actctacatc
     3901 ccacccactg ctcctctcct attcaccctt tcccacaatc tttcccccat ccccctagcc
     3961 ccagcaaagg tatccatcat tggctgaaaa gtatacattt ttcagcatgg agcattactg
     4021 ctgacaaaaa cccactttag gaagtaagat ttcttctctt attcatagaa tgattcatgt
     4081 cacacacact tcttttttga ctcacacggt acgtcacaac aggctttgta aggcttataa
     4141 ttactgaggc tatttttgtg tctctattca ttgctttccc ccacccgcct tctctgcatt
     4201 aataaccagt ccatcccccc cccctcatta ttcagtacgc agcaaaatag aactggtgtc
     4261 agccaatcag cctcttgtat ggtgactcat ttttaggcag aagcttaaaa acattggtcc
     4321 ttgtcaacag aaattaagcc tgctgctacc ctattcctga ggtagatcca ggaaaactct
     4381 gaaatagaat gggaacagat ctcatatcta attagatcct ttctatctct gagaattgta
     4441 ttcagtgcaa tcattactgc cacctgcttg acagtgtgta caatactttg tttaagcttc
     4501 cccactactg gatccattta acagaccaaa ccactgaggG Gcgcgccagc tagtaacagt
     4561 tggcattggg actagaaggc ctcattccta aaccagcaga tttttccatt gtaccaggaa
     4621 aacaaaactg caaatagcat aagtcctctg attttactgt ctccagagaa aatcatatcc
     4681 tcaatgcctg ggtctttcca tctggaattt ggcaactctg ctgatccctt ctcatagtgg
     4741 gaactcttgt aggaagtcta agagcaatta ttttggggat atatgctatt tttatgtctt
     4801 cttgtacatc accttctttg gtcaggagga aattagaatt ctggaaatac gtttaattat
     4861 taaaaggaca agccttggct tcaagtagtt gcaaatatga attattagtc catgtcatga
     4921 aagggctgga gaactacaaa gaaacaatga taatctattt gtcacaccca attaagatct
     4981 gccctggcct gggagtaaaa gagcattaaa tctgtgagtg gtaacttcaa gcagcatgtc
     5041 tatatttggg taattctgaa catgcctgga tttggtacct tacagcatcc tctaaaaatg
     5101 tactacttta gaaagtgttt tgagattttg ttgccttcgt gattgcagtt tgtgtgtgtg
     5161 tgtgtgtgtg gggggtgtta tttgagcttt tgagtttcca tgtagccaaa gaaagaaagt
     5221 ctaggaagaa atggtagact caaggtcaaa ttcaacaagg ggaaacttct ttgtggtgag
     5281 tctagacaac aaagccatta gcccagtgtt cacccccctg cctacaccct accttttcca
     5341 ttgatgaata ttcttacctg agccatcctc gcttctgagt cctgctctgg gtcctgtgga
     5401 gactgaatat cctgtctgta gtttttaatc cttgaagaga tttttcatga atagaacttt
     5461 ccttataatt tagcaacact gtccttgaaa acccccacaa ctatttacat aacagaagct
     5521 attgtttgaa gtgcaaaaca ggtcagaatt tggttcccag agattaatca ctgcagtcta
     5581 ttttgtatta tcataataat ttcatttatt ttgatacaat ttgttttcag aatcaacccc
     5641 agcttctttg cacaagtgac aaatccattt gtttctggta gaatgggtaa tgtgattaat
     5701 gaccttgcaa caggatttct ttaaaaaggg ggaacttttt ttttttcatt tttaattcac
     5761 agtaaaccag ctgagtctgt taagtgtgta actgttctga acttgtaagc catgggtaag
     5821 attttgaaat accccctccc aatcttttgc ggggtttctg ggtattaata ttaggctttc
     5881 tccaagaatc tgatgatact agtctccagg tgtaaagtgg acttgttgga ttcccgcatt
     5941 aaaaagaacg gggccccagt ctttgccatg gccccatcat gtttattccg gctggatgtt
     6001 ctcatcaaac cgaggtcatg cgaaataaat aaggtcatgc ctagaaggat attgtccccg
     6061 cacaagaacc ttcgagtgaa ctcgtgacta ctgcctcctg gtcttctgtg tgggcatccc
     6121 cttctttctt gcagcatcca ggacccactt ctgtccctgc tggtctgttt ctttctttct
     6181 ttctttcttt ctttctttct ttcttgtttt cttccgtttt tccccttgag ttctctgggg
     6241 ttgggcctgg gattttgggt acgcctacag actgtccgcc tctgcaatcg accgtcgtgc
     6301 ttcccgcgcc agaacaagga agcccacgcg agccgcgtag gtggctcgcg tggaagaccc
     6361 gggttcgagt cccgctggga gaattcctcc taggcaagtg gagtcatctc ggtcaggatt
     6421 tctcagccgg aagccgcgcc aacacctctc atcgtgggcg gcaagttgtc cccggggctt
     6481 ctttgaccgt gcggggccag ccgacgcttt ctctgaccgt ttccgaagcc cagtttcctt
     6541 tcctaaggag ccttggggat cctgcactcg cgtctctgaa acccaaccca cgacagaacc
     6601 cagccgcctg ggccagctta gcaatgcgcc cgcgccccgc cctccgcgtg acgcgaacgc
     6661 cggcggacca atcagagagc gcggcgcgag ccccacgcgc ctgtggcccc accctccaag
     6721 cttaagaaag ggcgcgcggg cagagcccgg gccagaacgc cgcgcaaagc ggagagcggg
     6781 tttttgcgac ggcaggcggc gcttgtgctg ccgtttccgt gctgcacgcg gttccctcga
     6841 gtgccgcgct cggacagacg tagccggccg cgaaggcgcc cagacggcaa accagcgacc
     6901 catgctgaag tgagcacaca ggtcagcgag gatatcaaga tctggcctcg gcggccaagc
     6961 ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg
     7021 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
     7081 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     7141 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
     7201 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     7261 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     7321 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     7381 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     7441 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     7501 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     7561 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     7621 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     7681 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     7741 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     7801 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     7861 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     7921 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     7981 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     8041 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     8101 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggc
//