LOCUS       pGL4.11_Nued2_E2        8424 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pGL4.11_Nued2_E2.gb
FEATURES             Location/Qualifiers
     CDS             546..665
                     /label="hPEST"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    717..838
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(1257..1845)
                     /label="ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2045..2836)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(2837..2905)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     polyA_signal    3010..3058
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3072..3163
                     /label="pause site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3993..4783
                     /label="mIgf1 Enhancer 6"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc-feature    complement(4052..4058)
                     /label="AP-1 BS, var 2.2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     iDNA            4246..4584
                     /label="DNAse1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4319..4325
                     /label="AP-1 BS, var 2.1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4319..4325
                     /label="AP-1 BS, var 1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4319..4325)
                     /label="AP-1 BS, var 1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(4319..4325)
                     /label="AP-1 BS, var 2.1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4367..4373
                     /label="AP-1 BS, var 2.5"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4867..4872
                     /label="Multiple cloning site for enhancers"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      7311..7320
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             7317..542
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc
       61 tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
      121 ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg
      181 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
      241 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
      301 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
      361 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
      421 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
      481 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
      541 tgaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga
      601 gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca
      661 acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag
      721 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      781 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      841 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      901 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
      961 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     1021 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     1081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1981 atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca
     2041 gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt
     2101 ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag
     2161 gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa
     2221 tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca
     2281 tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc
     2341 gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt
     2401 cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa
     2461 atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt
     2521 cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct
     2581 tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa
     2641 gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag
     2701 tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga
     2761 gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca
     2821 ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg
     2881 cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga agcatttatc
     2941 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
     3001 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     3061 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     3121 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa ctggccggta
     3181 cctgagctcg ctagcttcct ggcttgtagt gacctcgtct attgctagaa atcagaaggt
     3241 accactgagc cttcatttgt ttggtgaaat catggagtta cattaacaag ccgtttcccc
     3301 aaactatggc tctctaccca cttgtaatac acccgtgcac aggaacattt tctgaaagtc
     3361 aatgtttcct atcacttgct ccaaaattaa accagagata catcattcag gaagaagcca
     3421 gatgcactga agaaagaaaa acctgcttta tcagaagggg caagcttctc tcacatccca
     3481 tagcaattta aattcatttg ccggaatgtt aaatacaacc agtcttttag ttgggtccct
     3541 tcacataaac accaagttag cataaatgca aaatagttca catgattaaa tctttataac
     3601 accataattt atcttatgta cacacacaca cacagagaaa gagagagaga gagagagaga
     3661 gagagatctt ttacttgctg atacttctac attttttctg gagttctcaa ctctcatcat
     3721 ctctctaagg aagctggctt gtattgcccc agactcttag tgtatttagt tctaaaggat
     3781 agtatacaga ataaagggaa attttaattt tgtttttaat aggacctata taattcaggc
     3841 ctagctctgg tggagagaag gggaggagag gtccattagc atttattgtg cacctgcttt
     3901 atgctaaaca tgtctggaaa cagtgtctca cacattccca catcacagaa gggcagagac
     3961 tgcatgcctc agctccacct ccaactctgt gttcaataca ggacaggcaa tacctaccca
     4021 catggtaggt gtgtggaaat tttttattaa atgagtaatt gggagataca tactaatgaa
     4081 aaggtccagg aactgatatt ttctggatcc gtgttccaga aatcagacat atatatctcc
     4141 taagtaaaca caatcagttt caaaattata ataaactgga gggattttct ggattttgaa
     4201 atatcaatat agctacatgt gcatgctatt cagatattat taacatccaa agactttgaa
     4261 gacaataaag tgtatttaat acaggacttc gactaaaagt gagctcaatt ttctttgatg
     4321 attcagaagg tactcatgac caaataatac cctagggcca tcattatgca tcaattaccc
     4381 ttcccagcaa taactgctaa tagaaatttc tggtagttaa atgccaaatt aacaagctgt
     4441 tcctgccttt ggagcccatg tgcacgcaga ctctgccaga gagaaactga acgaagtatt
     4501 ttttgattgt actgctttta gggttttccc agtagagtaa tacaaaatct aattgctttt
     4561 gggtaaattt caactggttt acaacatgac tagttcctat acctgcctcc aaaaaagatg
     4621 tcatttatta tacatcatat attggcaatg ggtagcaaca ggcctgggct ctgctttttg
     4681 tgactgtgtg tggcttagag agagaaaaaa attacttcgc ttgtctcttg agaatttcag
     4741 tctttaactt cagattttta aatattgctg ttataattat catcattaat tatccatcca
     4801 tcagcaatga ggaagggtct tactatgtat cccaagctga cttccaaagc gcgtttaaac
     4861 gtgtGGcgcg ccagctagta acagttggca ttgggactag aaggcctcat tcctaaacca
     4921 gcagattttt ccattgtacc aggaaaacaa aactgcaaat agcataagtc ctctgatttt
     4981 actgtctcca gagaaaatca tatcctcaat gcctgggtct ttccatctgg aatttggcaa
     5041 ctctgctgat cccttctcat agtgggaact cttgtaggaa gtctaagagc aattattttg
     5101 gggatatatg ctatttttat gtcttcttgt acatcacctt ctttggtcag gaggaaatta
     5161 gaattctgga aatacgttta attattaaaa ggacaagcct tggcttcaag tagttgcaaa
     5221 tatgaattat tagtccatgt catgaaaggg ctggagaact acaaagaaac aatgataatc
     5281 tatttgtcac acccaattaa gatctgccct ggcctgggag taaaagagca ttaaatctgt
     5341 gagtggtaac ttcaagcagc atgtctatat ttgggtaatt ctgaacatgc ctggatttgg
     5401 taccttacag catcctctaa aaatgtacta ctttagaaag tgttttgaga ttttgttgcc
     5461 ttcgtgattg cagtttgtgt gtgtgtgtgt gtgtgggggg tgttatttga gcttttgagt
     5521 ttccatgtag ccaaagaaag aaagtctagg aagaaatggt agactcaagg tcaaattcaa
     5581 caaggggaaa cttctttgtg gtgagtctag acaacaaagc cattagccca gtgttcaccc
     5641 ccctgcctac accctacctt ttccattgat gaatattctt acctgagcca tcctcgcttc
     5701 tgagtcctgc tctgggtcct gtggagactg aatatcctgt ctgtagtttt taatccttga
     5761 agagattttt catgaataga actttcctta taatttagca acactgtcct tgaaaacccc
     5821 cacaactatt tacataacag aagctattgt ttgaagtgca aaacaggtca gaatttggtt
     5881 cccagagatt aatcactgca gtctattttg tattatcata ataatttcat ttattttgat
     5941 acaatttgtt ttcagaatca accccagctt ctttgcacaa gtgacaaatc catttgtttc
     6001 tggtagaatg ggtaatgtga ttaatgacct tgcaacagga tttctttaaa aagggggaac
     6061 tttttttttt tcatttttaa ttcacagtaa accagctgag tctgttaagt gtgtaactgt
     6121 tctgaacttg taagccatgg gtaagatttt gaaatacccc ctcccaatct tttgcggggt
     6181 ttctgggtat taatattagg ctttctccaa gaatctgatg atactagtct ccaggtgtaa
     6241 agtggacttg ttggattccc gcattaaaaa gaacggggcc ccagtctttg ccatggcccc
     6301 atcatgttta ttccggctgg atgttctcat caaaccgagg tcatgcgaaa taaataaggt
     6361 catgcctaga aggatattgt ccccgcacaa gaaccttcga gtgaactcgt gactactgcc
     6421 tcctggtctt ctgtgtgggc atccccttct ttcttgcagc atccaggacc cacttctgtc
     6481 cctgctggtc tgtttctttc tttctttctt tctttctttc tttctttctt gttttcttcc
     6541 gtttttcccc ttgagttctc tggggttggg cctgggattt tgggtacgcc tacagactgt
     6601 ccgcctctgc aatcgaccgt cgtgcttccc gcgccagaac aaggaagccc acgcgagccg
     6661 cgtaggtggc tcgcgtggaa gacccgggtt cgagtcccgc tgggagaatt cctcctaggc
     6721 aagtggagtc atctcggtca ggatttctca gccggaagcc gcgccaacac ctctcatcgt
     6781 gggcggcaag ttgtccccgg ggcttctttg accgtgcggg gccagccgac gctttctctg
     6841 accgtttccg aagcccagtt tcctttccta aggagccttg gggatcctgc actcgcgtct
     6901 ctgaaaccca acccacgaca gaacccagcc gcctgggcca gcttagcaat gcgcccgcgc
     6961 cccgccctcc gcgtgacgcg aacgccggcg gaccaatcag agagcgcggc gcgagcccca
     7021 cgcgcctgtg gccccaccct ccaagcttaa gaaagggcgc gcgggcagag cccgggccag
     7081 aacgccgcgc aaagcggaga gcgggttttt gcgacggcag gcggcgcttg tgctgccgtt
     7141 tccgtgctgc acgcggttcc ctcgagtgcc gcgctcggac agacgtagcc ggccgcgaag
     7201 gcgcccagac ggcaaaccag cgacccatgc tgaagtgagc acacaggtca gcgaggatat
     7261 caagatctgg cctcggcggc caagcttggc aatccggtac tgttggtaaa gccaccatgg
     7321 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
     7381 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
     7441 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
     7501 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
     7561 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
     7621 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
     7681 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     7741 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     7801 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     7861 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     7921 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     7981 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     8041 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     8101 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     8161 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     8221 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     8281 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     8341 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     8401 taggcaaggt ggtgcccttc ttcg
//